Influence of process parameters on the immobilization of commercial porcine pancreatic lipase using three low-cost supports

Detalhes bibliográficos
Autor(a) principal: Scherer, Robison P.
Data de Publicação: 2012
Outros Autores: Dallago, Rogério L., Penna, Fábio G., Bertella, Francile, Oliveira, Débora de, Oliveira, Jose Vladimir de, Pergher, Sibele Berenice Castellã
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFRN
Texto Completo: https://repositorio.ufrn.br/jspui/handle/123456789/29186
Resumo: The use of lipases as biocatalysts has attracted much attention recently, but industrial application has been hindered mainly due to the high production cost, determined by the production yield and enzyme stability. Immobilization of biocatalysts in inert supports can ensure their use for several batches, making possible to build cost-effective processes. The objective of this work was to investigate the influence of immobilization time and enzyme to support mass ratio on the yield of immobilization and esterification activity of porcine pancreatic lipase. Higher immobilization yields (38.2%) were obtained for pillared montmorillonite, 120 min of immobilization and enzyme to support mass ratio of 2:0.5. The highest esterification activity (1403 U/g) was achieved using the montmorillonite, after 180 min of immobilization and enzyme to support ratio of 2:1. The characterization of the supports, free and immobilized enzyme on different supports made possible to elucidate the immobilization results through the knowledge of support features.
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spelling Scherer, Robison P.Dallago, Rogério L.Penna, Fábio G.Bertella, FrancileOliveira, Débora deOliveira, Jose Vladimir dePergher, Sibele Berenice Castellã2020-06-09T01:40:50Z2020-06-09T01:40:50Z2012-10SCHERER, Robison P.; DALLAGO, Rogério L.; PENNA, Fábio G.; BERTELLA, Francile; OLIVEIRA, Débora de; OLIVEIRA, Jose Vladimir de; PERGHER, Sibele Berenice Castellã. Influence of process parameters on the immobilization of commercial porcine pancreatic lipase using three low-cost supports. Biocatalysis And Agricultural Biotechnology, [s. l.], v. 1, n. 4, p. 290-294, out. 2012. ISSN 1878-8181. DOI https://doi.org/10.1016/j.bcab.2012.06.003. Disponível em: https://www.sciencedirect.com/science/article/abs/pii/S1878818112001004. Acesso em: 08 jun. 2020.1878-8181https://repositorio.ufrn.br/jspui/handle/123456789/2918610.1016/j.bcab.2012.06.003ElsevierPorcine pancreatic lipaseImmobilizationKSFNatural montmorillonitePillared montmorilloniteInfluence of process parameters on the immobilization of commercial porcine pancreatic lipase using three low-cost supportsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleThe use of lipases as biocatalysts has attracted much attention recently, but industrial application has been hindered mainly due to the high production cost, determined by the production yield and enzyme stability. Immobilization of biocatalysts in inert supports can ensure their use for several batches, making possible to build cost-effective processes. The objective of this work was to investigate the influence of immobilization time and enzyme to support mass ratio on the yield of immobilization and esterification activity of porcine pancreatic lipase. Higher immobilization yields (38.2%) were obtained for pillared montmorillonite, 120 min of immobilization and enzyme to support mass ratio of 2:0.5. The highest esterification activity (1403 U/g) was achieved using the montmorillonite, after 180 min of immobilization and enzyme to support ratio of 2:1. The characterization of the supports, free and immobilized enzyme on different supports made possible to elucidate the immobilization results through the knowledge of support features.engreponame:Repositório Institucional da UFRNinstname:Universidade Federal do Rio Grande do Norte (UFRN)instacron:UFRNinfo:eu-repo/semantics/openAccessCC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8914https://repositorio.ufrn.br/bitstream/123456789/29186/2/license_rdf4d2950bda3d176f570a9f8b328dfbbefMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81484https://repositorio.ufrn.br/bitstream/123456789/29186/3/license.txte9597aa2854d128fd968be5edc8a28d9MD53TEXTInfluenceProcessParametersImmobilization_Pergher_2012.pdf.txtInfluenceProcessParametersImmobilization_Pergher_2012.pdf.txtExtracted texttext/plain26949https://repositorio.ufrn.br/bitstream/123456789/29186/4/InfluenceProcessParametersImmobilization_Pergher_2012.pdf.txt86a054a634665a5818ea8065f22c15aaMD54THUMBNAILInfluenceProcessParametersImmobilization_Pergher_2012.pdf.jpgInfluenceProcessParametersImmobilization_Pergher_2012.pdf.jpgGenerated Thumbnailimage/jpeg1804https://repositorio.ufrn.br/bitstream/123456789/29186/5/InfluenceProcessParametersImmobilization_Pergher_2012.pdf.jpg566b26e92a0630f01c1606b44caba77eMD55123456789/291862023-01-26 14:50:24.937oai:https://repositorio.ufrn.br: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Repositório de PublicaçõesPUBhttp://repositorio.ufrn.br/oai/opendoar:2023-01-26T17:50:24Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)false
dc.title.pt_BR.fl_str_mv Influence of process parameters on the immobilization of commercial porcine pancreatic lipase using three low-cost supports
title Influence of process parameters on the immobilization of commercial porcine pancreatic lipase using three low-cost supports
spellingShingle Influence of process parameters on the immobilization of commercial porcine pancreatic lipase using three low-cost supports
Scherer, Robison P.
Porcine pancreatic lipase
Immobilization
KSF
Natural montmorillonite
Pillared montmorillonite
title_short Influence of process parameters on the immobilization of commercial porcine pancreatic lipase using three low-cost supports
title_full Influence of process parameters on the immobilization of commercial porcine pancreatic lipase using three low-cost supports
title_fullStr Influence of process parameters on the immobilization of commercial porcine pancreatic lipase using three low-cost supports
title_full_unstemmed Influence of process parameters on the immobilization of commercial porcine pancreatic lipase using three low-cost supports
title_sort Influence of process parameters on the immobilization of commercial porcine pancreatic lipase using three low-cost supports
author Scherer, Robison P.
author_facet Scherer, Robison P.
Dallago, Rogério L.
Penna, Fábio G.
Bertella, Francile
Oliveira, Débora de
Oliveira, Jose Vladimir de
Pergher, Sibele Berenice Castellã
author_role author
author2 Dallago, Rogério L.
Penna, Fábio G.
Bertella, Francile
Oliveira, Débora de
Oliveira, Jose Vladimir de
Pergher, Sibele Berenice Castellã
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Scherer, Robison P.
Dallago, Rogério L.
Penna, Fábio G.
Bertella, Francile
Oliveira, Débora de
Oliveira, Jose Vladimir de
Pergher, Sibele Berenice Castellã
dc.subject.por.fl_str_mv Porcine pancreatic lipase
Immobilization
KSF
Natural montmorillonite
Pillared montmorillonite
topic Porcine pancreatic lipase
Immobilization
KSF
Natural montmorillonite
Pillared montmorillonite
description The use of lipases as biocatalysts has attracted much attention recently, but industrial application has been hindered mainly due to the high production cost, determined by the production yield and enzyme stability. Immobilization of biocatalysts in inert supports can ensure their use for several batches, making possible to build cost-effective processes. The objective of this work was to investigate the influence of immobilization time and enzyme to support mass ratio on the yield of immobilization and esterification activity of porcine pancreatic lipase. Higher immobilization yields (38.2%) were obtained for pillared montmorillonite, 120 min of immobilization and enzyme to support mass ratio of 2:0.5. The highest esterification activity (1403 U/g) was achieved using the montmorillonite, after 180 min of immobilization and enzyme to support ratio of 2:1. The characterization of the supports, free and immobilized enzyme on different supports made possible to elucidate the immobilization results through the knowledge of support features.
publishDate 2012
dc.date.issued.fl_str_mv 2012-10
dc.date.accessioned.fl_str_mv 2020-06-09T01:40:50Z
dc.date.available.fl_str_mv 2020-06-09T01:40:50Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.citation.fl_str_mv SCHERER, Robison P.; DALLAGO, Rogério L.; PENNA, Fábio G.; BERTELLA, Francile; OLIVEIRA, Débora de; OLIVEIRA, Jose Vladimir de; PERGHER, Sibele Berenice Castellã. Influence of process parameters on the immobilization of commercial porcine pancreatic lipase using three low-cost supports. Biocatalysis And Agricultural Biotechnology, [s. l.], v. 1, n. 4, p. 290-294, out. 2012. ISSN 1878-8181. DOI https://doi.org/10.1016/j.bcab.2012.06.003. Disponível em: https://www.sciencedirect.com/science/article/abs/pii/S1878818112001004. Acesso em: 08 jun. 2020.
dc.identifier.uri.fl_str_mv https://repositorio.ufrn.br/jspui/handle/123456789/29186
dc.identifier.issn.none.fl_str_mv 1878-8181
dc.identifier.doi.none.fl_str_mv 10.1016/j.bcab.2012.06.003
identifier_str_mv SCHERER, Robison P.; DALLAGO, Rogério L.; PENNA, Fábio G.; BERTELLA, Francile; OLIVEIRA, Débora de; OLIVEIRA, Jose Vladimir de; PERGHER, Sibele Berenice Castellã. Influence of process parameters on the immobilization of commercial porcine pancreatic lipase using three low-cost supports. Biocatalysis And Agricultural Biotechnology, [s. l.], v. 1, n. 4, p. 290-294, out. 2012. ISSN 1878-8181. DOI https://doi.org/10.1016/j.bcab.2012.06.003. Disponível em: https://www.sciencedirect.com/science/article/abs/pii/S1878818112001004. Acesso em: 08 jun. 2020.
1878-8181
10.1016/j.bcab.2012.06.003
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dc.publisher.none.fl_str_mv Elsevier
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