Influence of process parameters on the immobilization of commercial porcine pancreatic lipase using three low-cost supports
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRN |
Texto Completo: | https://repositorio.ufrn.br/jspui/handle/123456789/29186 |
Resumo: | The use of lipases as biocatalysts has attracted much attention recently, but industrial application has been hindered mainly due to the high production cost, determined by the production yield and enzyme stability. Immobilization of biocatalysts in inert supports can ensure their use for several batches, making possible to build cost-effective processes. The objective of this work was to investigate the influence of immobilization time and enzyme to support mass ratio on the yield of immobilization and esterification activity of porcine pancreatic lipase. Higher immobilization yields (38.2%) were obtained for pillared montmorillonite, 120 min of immobilization and enzyme to support mass ratio of 2:0.5. The highest esterification activity (1403 U/g) was achieved using the montmorillonite, after 180 min of immobilization and enzyme to support ratio of 2:1. The characterization of the supports, free and immobilized enzyme on different supports made possible to elucidate the immobilization results through the knowledge of support features. |
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Scherer, Robison P.Dallago, Rogério L.Penna, Fábio G.Bertella, FrancileOliveira, Débora deOliveira, Jose Vladimir dePergher, Sibele Berenice Castellã2020-06-09T01:40:50Z2020-06-09T01:40:50Z2012-10SCHERER, Robison P.; DALLAGO, Rogério L.; PENNA, Fábio G.; BERTELLA, Francile; OLIVEIRA, Débora de; OLIVEIRA, Jose Vladimir de; PERGHER, Sibele Berenice Castellã. Influence of process parameters on the immobilization of commercial porcine pancreatic lipase using three low-cost supports. Biocatalysis And Agricultural Biotechnology, [s. l.], v. 1, n. 4, p. 290-294, out. 2012. ISSN 1878-8181. DOI https://doi.org/10.1016/j.bcab.2012.06.003. Disponível em: https://www.sciencedirect.com/science/article/abs/pii/S1878818112001004. Acesso em: 08 jun. 2020.1878-8181https://repositorio.ufrn.br/jspui/handle/123456789/2918610.1016/j.bcab.2012.06.003ElsevierPorcine pancreatic lipaseImmobilizationKSFNatural montmorillonitePillared montmorilloniteInfluence of process parameters on the immobilization of commercial porcine pancreatic lipase using three low-cost supportsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleThe use of lipases as biocatalysts has attracted much attention recently, but industrial application has been hindered mainly due to the high production cost, determined by the production yield and enzyme stability. Immobilization of biocatalysts in inert supports can ensure their use for several batches, making possible to build cost-effective processes. The objective of this work was to investigate the influence of immobilization time and enzyme to support mass ratio on the yield of immobilization and esterification activity of porcine pancreatic lipase. Higher immobilization yields (38.2%) were obtained for pillared montmorillonite, 120 min of immobilization and enzyme to support mass ratio of 2:0.5. The highest esterification activity (1403 U/g) was achieved using the montmorillonite, after 180 min of immobilization and enzyme to support ratio of 2:1. The characterization of the supports, free and immobilized enzyme on different supports made possible to elucidate the immobilization results through the knowledge of support features.engreponame:Repositório Institucional da UFRNinstname:Universidade Federal do Rio Grande do Norte (UFRN)instacron:UFRNinfo:eu-repo/semantics/openAccessCC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8914https://repositorio.ufrn.br/bitstream/123456789/29186/2/license_rdf4d2950bda3d176f570a9f8b328dfbbefMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81484https://repositorio.ufrn.br/bitstream/123456789/29186/3/license.txte9597aa2854d128fd968be5edc8a28d9MD53TEXTInfluenceProcessParametersImmobilization_Pergher_2012.pdf.txtInfluenceProcessParametersImmobilization_Pergher_2012.pdf.txtExtracted texttext/plain26949https://repositorio.ufrn.br/bitstream/123456789/29186/4/InfluenceProcessParametersImmobilization_Pergher_2012.pdf.txt86a054a634665a5818ea8065f22c15aaMD54THUMBNAILInfluenceProcessParametersImmobilization_Pergher_2012.pdf.jpgInfluenceProcessParametersImmobilization_Pergher_2012.pdf.jpgGenerated Thumbnailimage/jpeg1804https://repositorio.ufrn.br/bitstream/123456789/29186/5/InfluenceProcessParametersImmobilization_Pergher_2012.pdf.jpg566b26e92a0630f01c1606b44caba77eMD55123456789/291862023-01-26 14:50:24.937oai:https://repositorio.ufrn.br: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Repositório de PublicaçõesPUBhttp://repositorio.ufrn.br/oai/opendoar:2023-01-26T17:50:24Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)false |
dc.title.pt_BR.fl_str_mv |
Influence of process parameters on the immobilization of commercial porcine pancreatic lipase using three low-cost supports |
title |
Influence of process parameters on the immobilization of commercial porcine pancreatic lipase using three low-cost supports |
spellingShingle |
Influence of process parameters on the immobilization of commercial porcine pancreatic lipase using three low-cost supports Scherer, Robison P. Porcine pancreatic lipase Immobilization KSF Natural montmorillonite Pillared montmorillonite |
title_short |
Influence of process parameters on the immobilization of commercial porcine pancreatic lipase using three low-cost supports |
title_full |
Influence of process parameters on the immobilization of commercial porcine pancreatic lipase using three low-cost supports |
title_fullStr |
Influence of process parameters on the immobilization of commercial porcine pancreatic lipase using three low-cost supports |
title_full_unstemmed |
Influence of process parameters on the immobilization of commercial porcine pancreatic lipase using three low-cost supports |
title_sort |
Influence of process parameters on the immobilization of commercial porcine pancreatic lipase using three low-cost supports |
author |
Scherer, Robison P. |
author_facet |
Scherer, Robison P. Dallago, Rogério L. Penna, Fábio G. Bertella, Francile Oliveira, Débora de Oliveira, Jose Vladimir de Pergher, Sibele Berenice Castellã |
author_role |
author |
author2 |
Dallago, Rogério L. Penna, Fábio G. Bertella, Francile Oliveira, Débora de Oliveira, Jose Vladimir de Pergher, Sibele Berenice Castellã |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Scherer, Robison P. Dallago, Rogério L. Penna, Fábio G. Bertella, Francile Oliveira, Débora de Oliveira, Jose Vladimir de Pergher, Sibele Berenice Castellã |
dc.subject.por.fl_str_mv |
Porcine pancreatic lipase Immobilization KSF Natural montmorillonite Pillared montmorillonite |
topic |
Porcine pancreatic lipase Immobilization KSF Natural montmorillonite Pillared montmorillonite |
description |
The use of lipases as biocatalysts has attracted much attention recently, but industrial application has been hindered mainly due to the high production cost, determined by the production yield and enzyme stability. Immobilization of biocatalysts in inert supports can ensure their use for several batches, making possible to build cost-effective processes. The objective of this work was to investigate the influence of immobilization time and enzyme to support mass ratio on the yield of immobilization and esterification activity of porcine pancreatic lipase. Higher immobilization yields (38.2%) were obtained for pillared montmorillonite, 120 min of immobilization and enzyme to support mass ratio of 2:0.5. The highest esterification activity (1403 U/g) was achieved using the montmorillonite, after 180 min of immobilization and enzyme to support ratio of 2:1. The characterization of the supports, free and immobilized enzyme on different supports made possible to elucidate the immobilization results through the knowledge of support features. |
publishDate |
2012 |
dc.date.issued.fl_str_mv |
2012-10 |
dc.date.accessioned.fl_str_mv |
2020-06-09T01:40:50Z |
dc.date.available.fl_str_mv |
2020-06-09T01:40:50Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
SCHERER, Robison P.; DALLAGO, Rogério L.; PENNA, Fábio G.; BERTELLA, Francile; OLIVEIRA, Débora de; OLIVEIRA, Jose Vladimir de; PERGHER, Sibele Berenice Castellã. Influence of process parameters on the immobilization of commercial porcine pancreatic lipase using three low-cost supports. Biocatalysis And Agricultural Biotechnology, [s. l.], v. 1, n. 4, p. 290-294, out. 2012. ISSN 1878-8181. DOI https://doi.org/10.1016/j.bcab.2012.06.003. Disponível em: https://www.sciencedirect.com/science/article/abs/pii/S1878818112001004. Acesso em: 08 jun. 2020. |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufrn.br/jspui/handle/123456789/29186 |
dc.identifier.issn.none.fl_str_mv |
1878-8181 |
dc.identifier.doi.none.fl_str_mv |
10.1016/j.bcab.2012.06.003 |
identifier_str_mv |
SCHERER, Robison P.; DALLAGO, Rogério L.; PENNA, Fábio G.; BERTELLA, Francile; OLIVEIRA, Débora de; OLIVEIRA, Jose Vladimir de; PERGHER, Sibele Berenice Castellã. Influence of process parameters on the immobilization of commercial porcine pancreatic lipase using three low-cost supports. Biocatalysis And Agricultural Biotechnology, [s. l.], v. 1, n. 4, p. 290-294, out. 2012. ISSN 1878-8181. DOI https://doi.org/10.1016/j.bcab.2012.06.003. Disponível em: https://www.sciencedirect.com/science/article/abs/pii/S1878818112001004. Acesso em: 08 jun. 2020. 1878-8181 10.1016/j.bcab.2012.06.003 |
url |
https://repositorio.ufrn.br/jspui/handle/123456789/29186 |
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eng |
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eng |
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info:eu-repo/semantics/openAccess |
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Elsevier |
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Elsevier |
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