Identification of non-zein proteins in BR473 maize protein bodies by LC-nanoESI-MS/MS

Detalhes bibliográficos
Autor(a) principal: Bicudo, Tatiana de Campos
Data de Publicação: 2009
Outros Autores: Bicudo, Rogério Campos, Forato, Lucimara A., Titato, Guilherme M., Colnago, Luiz A., Lanças, Fernando M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFRN
Texto Completo: https://repositorio.ufrn.br/handle/123456789/30364
Resumo: The nutritional value of maize seed is limited due to its high content of storage proteins (zeins), which are deficient in essential amino acids such as lysine and tryptophan. In a previous paper, we showed that protein bodies obtained from BR473 maize variety, developed by Embrapa (Brazilian Agricultural Research Corporation), were mainly constituted by Z27 and a smaller quantity of Z50g-zeins. Besides zein proteins, other not identified protein band in the SDS/PAGE was also observed, which could indicate the presence of non-zein proteins additionally tog-zeins. In the present paper, we have demonstrated the presence of non-zein proteins in BR473 maize protein bodies by LCnanoESI-MS/MS and database searching. This fact could be related to the excellent energetic value and higher protein quality of BR473 maize grains, since high lysine concentration in some maize varieties has been related to the presence of cytoskeleton proteins that are non-zeins. We have identified the following proteins: Brittle-1 protein (chloroplast precursor), Legumin-1, glyceroldehyde-3-phosphate dehydrogenase, and elongation factor 1-a
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spelling Bicudo, Tatiana de CamposBicudo, Rogério CamposForato, Lucimara A.Titato, Guilherme M.Colnago, Luiz A.Lanças, Fernando M.2020-10-09T19:36:35Z2020-10-09T19:36:35Z2009-12-22BICUDO, Rogério Campos; BICUDO, Tatiana Campos; FORATO, Lucimara A.; TITATO, Guilherme M.; COLNAGO, Luiz A.; LANÇAS, Fernando M.. Identification of non-zein proteins in BR473 maize protein bodies by LC-nanoESI-MS/MS. Journal Of Separation Science, [S.L.], v. 32, n. 21, p. 3579-3584, 7 out. 2009. Disponível em: https://onlinelibrary.wiley.com/doi/full/10.1002/jssc.200900339. Acesso em: 08 set. 2020. http://dx.doi.org/10.1002/jssc.2009003391615-9314https://repositorio.ufrn.br/handle/123456789/3036410.1002/jssc.200900339WileyAttribution 3.0 Brazilhttp://creativecommons.org/licenses/by/3.0/br/info:eu-repo/semantics/openAccessBR473 maize protein bodiesLC-nanoESI-MS/MSNon-zein proteinsProtein identificationIdentification of non-zein proteins in BR473 maize protein bodies by LC-nanoESI-MS/MSinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleThe nutritional value of maize seed is limited due to its high content of storage proteins (zeins), which are deficient in essential amino acids such as lysine and tryptophan. In a previous paper, we showed that protein bodies obtained from BR473 maize variety, developed by Embrapa (Brazilian Agricultural Research Corporation), were mainly constituted by Z27 and a smaller quantity of Z50g-zeins. Besides zein proteins, other not identified protein band in the SDS/PAGE was also observed, which could indicate the presence of non-zein proteins additionally tog-zeins. In the present paper, we have demonstrated the presence of non-zein proteins in BR473 maize protein bodies by LCnanoESI-MS/MS and database searching. This fact could be related to the excellent energetic value and higher protein quality of BR473 maize grains, since high lysine concentration in some maize varieties has been related to the presence of cytoskeleton proteins that are non-zeins. We have identified the following proteins: Brittle-1 protein (chloroplast precursor), Legumin-1, glyceroldehyde-3-phosphate dehydrogenase, and elongation factor 1-aengreponame:Repositório Institucional da UFRNinstname:Universidade Federal do Rio Grande do Norte (UFRN)instacron:UFRNCC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8914https://repositorio.ufrn.br/bitstream/123456789/30364/2/license_rdf4d2950bda3d176f570a9f8b328dfbbefMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81484https://repositorio.ufrn.br/bitstream/123456789/30364/3/license.txte9597aa2854d128fd968be5edc8a28d9MD53TEXTIdentificationNonzeinProteinsBicudo2009.pdf.txtIdentificationNonzeinProteinsBicudo2009.pdf.txtExtracted texttext/plain24038https://repositorio.ufrn.br/bitstream/123456789/30364/4/IdentificationNonzeinProteinsBicudo2009.pdf.txt89689a020e28be2f25971fd3c30f28ebMD54THUMBNAILIdentificationNonzeinProteinsBicudo2009.pdf.jpgIdentificationNonzeinProteinsBicudo2009.pdf.jpgGenerated Thumbnailimage/jpeg2369https://repositorio.ufrn.br/bitstream/123456789/30364/5/IdentificationNonzeinProteinsBicudo2009.pdf.jpg7bc0adf83e9a3d539a4ae985a9dc3daeMD55123456789/303642022-05-25 15:41:48.294oai:https://repositorio.ufrn.br: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Repositório de PublicaçõesPUBhttp://repositorio.ufrn.br/oai/opendoar:2022-05-25T18:41:48Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)false
dc.title.pt_BR.fl_str_mv Identification of non-zein proteins in BR473 maize protein bodies by LC-nanoESI-MS/MS
title Identification of non-zein proteins in BR473 maize protein bodies by LC-nanoESI-MS/MS
spellingShingle Identification of non-zein proteins in BR473 maize protein bodies by LC-nanoESI-MS/MS
Bicudo, Tatiana de Campos
BR473 maize protein bodies
LC-nanoESI-MS/MS
Non-zein proteins
Protein identification
title_short Identification of non-zein proteins in BR473 maize protein bodies by LC-nanoESI-MS/MS
title_full Identification of non-zein proteins in BR473 maize protein bodies by LC-nanoESI-MS/MS
title_fullStr Identification of non-zein proteins in BR473 maize protein bodies by LC-nanoESI-MS/MS
title_full_unstemmed Identification of non-zein proteins in BR473 maize protein bodies by LC-nanoESI-MS/MS
title_sort Identification of non-zein proteins in BR473 maize protein bodies by LC-nanoESI-MS/MS
author Bicudo, Tatiana de Campos
author_facet Bicudo, Tatiana de Campos
Bicudo, Rogério Campos
Forato, Lucimara A.
Titato, Guilherme M.
Colnago, Luiz A.
Lanças, Fernando M.
author_role author
author2 Bicudo, Rogério Campos
Forato, Lucimara A.
Titato, Guilherme M.
Colnago, Luiz A.
Lanças, Fernando M.
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Bicudo, Tatiana de Campos
Bicudo, Rogério Campos
Forato, Lucimara A.
Titato, Guilherme M.
Colnago, Luiz A.
Lanças, Fernando M.
dc.subject.por.fl_str_mv BR473 maize protein bodies
LC-nanoESI-MS/MS
Non-zein proteins
Protein identification
topic BR473 maize protein bodies
LC-nanoESI-MS/MS
Non-zein proteins
Protein identification
description The nutritional value of maize seed is limited due to its high content of storage proteins (zeins), which are deficient in essential amino acids such as lysine and tryptophan. In a previous paper, we showed that protein bodies obtained from BR473 maize variety, developed by Embrapa (Brazilian Agricultural Research Corporation), were mainly constituted by Z27 and a smaller quantity of Z50g-zeins. Besides zein proteins, other not identified protein band in the SDS/PAGE was also observed, which could indicate the presence of non-zein proteins additionally tog-zeins. In the present paper, we have demonstrated the presence of non-zein proteins in BR473 maize protein bodies by LCnanoESI-MS/MS and database searching. This fact could be related to the excellent energetic value and higher protein quality of BR473 maize grains, since high lysine concentration in some maize varieties has been related to the presence of cytoskeleton proteins that are non-zeins. We have identified the following proteins: Brittle-1 protein (chloroplast precursor), Legumin-1, glyceroldehyde-3-phosphate dehydrogenase, and elongation factor 1-a
publishDate 2009
dc.date.issued.fl_str_mv 2009-12-22
dc.date.accessioned.fl_str_mv 2020-10-09T19:36:35Z
dc.date.available.fl_str_mv 2020-10-09T19:36:35Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.citation.fl_str_mv BICUDO, Rogério Campos; BICUDO, Tatiana Campos; FORATO, Lucimara A.; TITATO, Guilherme M.; COLNAGO, Luiz A.; LANÇAS, Fernando M.. Identification of non-zein proteins in BR473 maize protein bodies by LC-nanoESI-MS/MS. Journal Of Separation Science, [S.L.], v. 32, n. 21, p. 3579-3584, 7 out. 2009. Disponível em: https://onlinelibrary.wiley.com/doi/full/10.1002/jssc.200900339. Acesso em: 08 set. 2020. http://dx.doi.org/10.1002/jssc.200900339
dc.identifier.uri.fl_str_mv https://repositorio.ufrn.br/handle/123456789/30364
dc.identifier.issn.none.fl_str_mv 1615-9314
dc.identifier.doi.none.fl_str_mv 10.1002/jssc.200900339
identifier_str_mv BICUDO, Rogério Campos; BICUDO, Tatiana Campos; FORATO, Lucimara A.; TITATO, Guilherme M.; COLNAGO, Luiz A.; LANÇAS, Fernando M.. Identification of non-zein proteins in BR473 maize protein bodies by LC-nanoESI-MS/MS. Journal Of Separation Science, [S.L.], v. 32, n. 21, p. 3579-3584, 7 out. 2009. Disponível em: https://onlinelibrary.wiley.com/doi/full/10.1002/jssc.200900339. Acesso em: 08 set. 2020. http://dx.doi.org/10.1002/jssc.200900339
1615-9314
10.1002/jssc.200900339
url https://repositorio.ufrn.br/handle/123456789/30364
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http://creativecommons.org/licenses/by/3.0/br/
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rights_invalid_str_mv Attribution 3.0 Brazil
http://creativecommons.org/licenses/by/3.0/br/
eu_rights_str_mv openAccess
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