Análise citogenética de células-tronco derivadas do subendotélio da veia umbilical humana

Detalhes bibliográficos
Autor(a) principal: Duarte, Denise de Medeiros
Data de Publicação: 2009
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Institucional da UFRN
Texto Completo: https://repositorio.ufrn.br/jspui/handle/123456789/16767
Resumo: Mesenchymal stem cells (MSCs) are known as a population of multi-potential cells able to proliferate and differentiate into multiple mesodermal tissues including bone, cartilage, muscle, ligament, tendon, fat and stroma. Several applications of the study of EC can be emphasized the therapeutic techniques such as guided bone regeneration by implantation of EC in the affected site, without the need for bone grafts, using titanium as a vehicle. The process of cryopreservation is essential for the maintenance of cell cultures, since the cell line is frozen, it can be maintained in liquid nitrogen for an indefinite period and then thawed for therapeutic or experimental purposes. The aim of this study was to isolate a population of MSCs derived from the subendothelium of the umbilical vein human (MSCs-SUVH) to assess cytogenetic analysis by the possibility of appearance of chromosomal changes in two different situations: MSCs-SUVH regarding the process of cryopreservation and MSCs-SUVH grown on the surface of titanium. Flow cytometry analysis revealed that, this cell population was positive for the markers CD29, CD73 and CD90, but there was no expression of hematopoietic lineage markers, such as CD14, CD34 and CD45 and demonstrated capacity for osteogenic differentiation. The chromosomes obtained from the primary culture of MSCs-SUVH were analyzed by GTW banding technique, and results are described as guidelines to ISCN 2005. There was not the emergence of clonal chromosomal changes in the MSCs-SUVH in different situations analyzed. However one of the strings presented a balanced paracentric inversion, probably a cytogenetic constitutional alterations, which was present before and after the experimental situations that the MSCs-SUVH was submitted
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spelling Duarte, Denise de Medeiroshttp://lattes.cnpq.br/4683071633811092http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4781004Y8Moura, Carlos Eduardo Bezerra dehttp://lattes.cnpq.br/4717410137206021Santos, Neidehttp://lattes.cnpq.br/2906973201323652Medeiros, Sílvia Regina Batistuzzo de2014-12-17T15:18:10Z2009-06-162014-12-17T15:18:10Z2009-02-26DUARTE, Denise de Medeiros. Análise citogenética de células-tronco derivadas do subendotélio da veia umbilical humana. 2009. 2 f. Dissertação (Mestrado em Genética e Biologia Molecular) - Universidade Federal do Rio Grande do Norte, Natal, 2009.https://repositorio.ufrn.br/jspui/handle/123456789/16767Mesenchymal stem cells (MSCs) are known as a population of multi-potential cells able to proliferate and differentiate into multiple mesodermal tissues including bone, cartilage, muscle, ligament, tendon, fat and stroma. Several applications of the study of EC can be emphasized the therapeutic techniques such as guided bone regeneration by implantation of EC in the affected site, without the need for bone grafts, using titanium as a vehicle. The process of cryopreservation is essential for the maintenance of cell cultures, since the cell line is frozen, it can be maintained in liquid nitrogen for an indefinite period and then thawed for therapeutic or experimental purposes. The aim of this study was to isolate a population of MSCs derived from the subendothelium of the umbilical vein human (MSCs-SUVH) to assess cytogenetic analysis by the possibility of appearance of chromosomal changes in two different situations: MSCs-SUVH regarding the process of cryopreservation and MSCs-SUVH grown on the surface of titanium. Flow cytometry analysis revealed that, this cell population was positive for the markers CD29, CD73 and CD90, but there was no expression of hematopoietic lineage markers, such as CD14, CD34 and CD45 and demonstrated capacity for osteogenic differentiation. The chromosomes obtained from the primary culture of MSCs-SUVH were analyzed by GTW banding technique, and results are described as guidelines to ISCN 2005. There was not the emergence of clonal chromosomal changes in the MSCs-SUVH in different situations analyzed. However one of the strings presented a balanced paracentric inversion, probably a cytogenetic constitutional alterations, which was present before and after the experimental situations that the MSCs-SUVH was submittedCélulas-tronco mesenquimais (CTMs) são conhecidas como uma população de células capazes de se proliferar e diferenciar em múltiplos tecidos de origem mesodérmico incluindo osso, cartilagem, músculo, ligamento, tendão, gordura e estroma. Das diversas aplicações do estudo das CTMs podem-se ressaltar as técnicas terapêuticas como a regeneração óssea guiada através da implantação de CTMs no local afetado, sem a necessidade de enxertos ósseos, usando o titânio como veículo. O processo de criopreservação é essencial para a manutenção de cultivos celulares, uma vez que a linhagem celular é congelada, a mesma pode ser mantida em nitrogênio líquido por tempo indeterminado e depois descongelado para fins experimentais ou terapêuticos. Deste modo, o objetivo desse trabalho foi isolar uma população de células-tronco mesenquimais derivadas do subendotélio da veia umbilical humana (CTMs-SVUH) para avaliar através da análise citogenética a possibilidade de surgimento de alterações cromossômicas em duas diferentes situações: CTMs-SVUH frente ao processo de criopreservação e CTMs-SVUH cultivadas sobre a superfície de titânio. Análise por citometria de fluxo revelou que, esta população celular foi positiva para os marcadores CD29, CD73 e CD90, mas foram negativas para marcadores de linhagem hematopoiético como, CD14, CD34 e CD45 e demonstraram capacidade de diferenciação osteogênica. Os cromossomos obtidos a partir da cultura primária de CTMs-SVUH foram analisados através da técnica de bandeamento GTW, e os resultados descritos conforme orientações do ISCN 2005. Não houve o aparecimento de alterações cromossômicas clonais nas CTMs-SVUH nas diferentes situações analisadas. Entretanto um dos cordões apresentou uma inversão paracêntrica balanceada, provavelmente uma alteração citogenética constitucional, que esteve presente antes e depois das situações experimentais a que as CTM-SVUH foram submetidasCoordenação de Aperfeiçoamento de Pessoal de Nível Superiorapplication/pdfporUniversidade Federal do Rio Grande do NortePrograma de Pós-Graduação em Genética e Biologia MolecularUFRNBRGenética e Biologia MolecularCélula-troncoCitogenéticaCriopreservaçãoTitânioStem cellsCytogeneticsCryopreservationTitaniumCNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA::BIOLOGIA MOLECULARAnálise citogenética de células-tronco derivadas do subendotélio da veia umbilical humanainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRNinstname:Universidade Federal do Rio Grande do Norte (UFRN)instacron:UFRNORIGINALDeniseMD.pdfapplication/pdf54846https://repositorio.ufrn.br/bitstream/123456789/16767/1/DeniseMD.pdf61beb5b55055ae5d403c739177202529MD51TEXTDeniseMD.pdf.txtDeniseMD.pdf.txtExtracted texttext/plain3995https://repositorio.ufrn.br/bitstream/123456789/16767/6/DeniseMD.pdf.txt02754e1ddc04a1bd13ae56afe32ecd50MD56THUMBNAILDeniseMD.pdf.jpgDeniseMD.pdf.jpgIM Thumbnailimage/jpeg6543https://repositorio.ufrn.br/bitstream/123456789/16767/7/DeniseMD.pdf.jpg1476b30b3433f60411a81c58d8184100MD57123456789/167672020-02-05 11:21:37.66oai:https://repositorio.ufrn.br:123456789/16767Repositório de PublicaçõesPUBhttp://repositorio.ufrn.br/oai/opendoar:2020-02-05T14:21:37Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)false
dc.title.por.fl_str_mv Análise citogenética de células-tronco derivadas do subendotélio da veia umbilical humana
title Análise citogenética de células-tronco derivadas do subendotélio da veia umbilical humana
spellingShingle Análise citogenética de células-tronco derivadas do subendotélio da veia umbilical humana
Duarte, Denise de Medeiros
Célula-tronco
Citogenética
Criopreservação
Titânio
Stem cells
Cytogenetics
Cryopreservation
Titanium
CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA::BIOLOGIA MOLECULAR
title_short Análise citogenética de células-tronco derivadas do subendotélio da veia umbilical humana
title_full Análise citogenética de células-tronco derivadas do subendotélio da veia umbilical humana
title_fullStr Análise citogenética de células-tronco derivadas do subendotélio da veia umbilical humana
title_full_unstemmed Análise citogenética de células-tronco derivadas do subendotélio da veia umbilical humana
title_sort Análise citogenética de células-tronco derivadas do subendotélio da veia umbilical humana
author Duarte, Denise de Medeiros
author_facet Duarte, Denise de Medeiros
author_role author
dc.contributor.authorID.por.fl_str_mv
dc.contributor.authorLattes.por.fl_str_mv http://lattes.cnpq.br/4683071633811092
dc.contributor.advisorID.por.fl_str_mv
dc.contributor.advisorLattes.por.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4781004Y8
dc.contributor.referees1.pt_BR.fl_str_mv Moura, Carlos Eduardo Bezerra de
dc.contributor.referees1ID.por.fl_str_mv
dc.contributor.referees1Lattes.por.fl_str_mv http://lattes.cnpq.br/4717410137206021
dc.contributor.referees2.pt_BR.fl_str_mv Santos, Neide
dc.contributor.referees2ID.por.fl_str_mv
dc.contributor.referees2Lattes.por.fl_str_mv http://lattes.cnpq.br/2906973201323652
dc.contributor.author.fl_str_mv Duarte, Denise de Medeiros
dc.contributor.advisor1.fl_str_mv Medeiros, Sílvia Regina Batistuzzo de
contributor_str_mv Medeiros, Sílvia Regina Batistuzzo de
dc.subject.por.fl_str_mv Célula-tronco
Citogenética
Criopreservação
Titânio
topic Célula-tronco
Citogenética
Criopreservação
Titânio
Stem cells
Cytogenetics
Cryopreservation
Titanium
CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA::BIOLOGIA MOLECULAR
dc.subject.eng.fl_str_mv Stem cells
Cytogenetics
Cryopreservation
Titanium
dc.subject.cnpq.fl_str_mv CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA::BIOLOGIA MOLECULAR
description Mesenchymal stem cells (MSCs) are known as a population of multi-potential cells able to proliferate and differentiate into multiple mesodermal tissues including bone, cartilage, muscle, ligament, tendon, fat and stroma. Several applications of the study of EC can be emphasized the therapeutic techniques such as guided bone regeneration by implantation of EC in the affected site, without the need for bone grafts, using titanium as a vehicle. The process of cryopreservation is essential for the maintenance of cell cultures, since the cell line is frozen, it can be maintained in liquid nitrogen for an indefinite period and then thawed for therapeutic or experimental purposes. The aim of this study was to isolate a population of MSCs derived from the subendothelium of the umbilical vein human (MSCs-SUVH) to assess cytogenetic analysis by the possibility of appearance of chromosomal changes in two different situations: MSCs-SUVH regarding the process of cryopreservation and MSCs-SUVH grown on the surface of titanium. Flow cytometry analysis revealed that, this cell population was positive for the markers CD29, CD73 and CD90, but there was no expression of hematopoietic lineage markers, such as CD14, CD34 and CD45 and demonstrated capacity for osteogenic differentiation. The chromosomes obtained from the primary culture of MSCs-SUVH were analyzed by GTW banding technique, and results are described as guidelines to ISCN 2005. There was not the emergence of clonal chromosomal changes in the MSCs-SUVH in different situations analyzed. However one of the strings presented a balanced paracentric inversion, probably a cytogenetic constitutional alterations, which was present before and after the experimental situations that the MSCs-SUVH was submitted
publishDate 2009
dc.date.available.fl_str_mv 2009-06-16
2014-12-17T15:18:10Z
dc.date.issued.fl_str_mv 2009-02-26
dc.date.accessioned.fl_str_mv 2014-12-17T15:18:10Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv DUARTE, Denise de Medeiros. Análise citogenética de células-tronco derivadas do subendotélio da veia umbilical humana. 2009. 2 f. Dissertação (Mestrado em Genética e Biologia Molecular) - Universidade Federal do Rio Grande do Norte, Natal, 2009.
dc.identifier.uri.fl_str_mv https://repositorio.ufrn.br/jspui/handle/123456789/16767
identifier_str_mv DUARTE, Denise de Medeiros. Análise citogenética de células-tronco derivadas do subendotélio da veia umbilical humana. 2009. 2 f. Dissertação (Mestrado em Genética e Biologia Molecular) - Universidade Federal do Rio Grande do Norte, Natal, 2009.
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dc.publisher.none.fl_str_mv Universidade Federal do Rio Grande do Norte
dc.publisher.program.fl_str_mv Programa de Pós-Graduação em Genética e Biologia Molecular
dc.publisher.initials.fl_str_mv UFRN
dc.publisher.country.fl_str_mv BR
dc.publisher.department.fl_str_mv Genética e Biologia Molecular
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