Immobilization of lipases via interfacial activation on hydrophobic supports: Production of biocatalysts libraries by altering the immobilization conditions

Detalhes bibliográficos
Autor(a) principal: Peña, Sara Arana
Data de Publicação: 2021
Outros Autores: Rios, Nathalia Saraiva, Carballares, Diego, Gonçalves, Luciana Rocha Barros, Lafuente, Roberto Fernandez
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFRN
Texto Completo: https://repositorio.ufrn.br/handle/123456789/45069
Resumo: 2030-12
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spelling Peña, Sara AranaRios, Nathalia SaraivaCarballares, DiegoGonçalves, Luciana Rocha BarrosLafuente, Roberto Fernandez2021-11-29T21:23:24Z2021-02-15ARANA-PEÑA, Sara; RIOS, Nathalia S.; CARBALLARES, Diego; GONÇALVES, Luciana R.B.; FERNANDEZ-LAFUENTE, Roberto. Immobilization of lipases via interfacial activation on hydrophobic supports: production of biocatalysts libraries by altering the immobilization conditions. Catalysis Today, [S.L.], v. 362, p. 130-140, fev. 2021. Disponível em: https://www.sciencedirect.com/science/article/abs/pii/S0920586120301814?via%3Dihub#!. Acesso em: 29 nov. 2021. https://doi.org/10.1016/j.cattod.2020.03.0590920-5861https://repositorio.ufrn.br/handle/123456789/4506910.1016/j.cattod.2020.03.059ElsevierLipase modulationLipase stabilityInterfacial activationLipase immobilizationLipase specificityImmobilization of lipases via interfacial activation on hydrophobic supports: Production of biocatalysts libraries by altering the immobilization conditionsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2030-12Lipases A and B from Candida antarctica (CALA, CALB), and those from Candida rugosa (CRL) and Rhizomucor miehei (RML) have been immobilized on octyl agarose beads under 16 different conditions. The activities of the biocatalysts versus different substrates were analyzed, as well as their thermal stabilities at pH 7.0. All CALB and CRL preparations showed very similar properties, except the CRL biocatalysts prepared at pH 9. Under these conditions the free enzyme was partially inactivated. Immobilized CALA showed some significant differences in activity depending on the immobilization conditions when using esters of mandelic acid as substrates (the activities of the different preparations differed by more than a 2-fold factor), while when using the other substrates the differences were minimal. However, immobilized RML enzyme greatly alters its activity depending on the immobilization conditions, reaching differences up to 4–5 fold in both activity and stability. It is remarkable that the effect of one immobilization variable depends on the substrates, and that there are strong interactions between the different immobilization variables. Thus, this immobilization method was very robust (producing almost identical functional biocatalysts independently from the immobilization conditions) using CRL or CALB, while the immobilization conditions must be carefully controlled using RML to have reproducible resultsengreponame:Repositório Institucional da UFRNinstname:Universidade Federal do Rio Grande do Norte (UFRN)instacron:UFRNinfo:eu-repo/semantics/openAccessCC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8914https://repositorio.ufrn.br/bitstream/123456789/45069/2/license_rdf4d2950bda3d176f570a9f8b328dfbbefMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81569https://repositorio.ufrn.br/bitstream/123456789/45069/3/license.txt6e6f57145bc87daf99079f06b081ff9fMD53123456789/450692024-03-19 01:06:10.765oai:https://repositorio.ufrn.br:123456789/45069TElDRU7Dh0HCoERFIERJU1RSSUJVScOHw4NPIE7Dg08tRVhDTFVTSVZBCgoKQW8gYXNzaW5hciBlIGVudHJlZ2FyIGVzdGHCoGxpY2Vuw6dhLCBvL2EgU3IuL1NyYS4gKGF1dG9yIG91IGRldGVudG9yIGRvcyBkaXJlaXRvcyBkZSBhdXRvcik6CgoKYSkgQ29uY2VkZSDDoCBVbml2ZXJzaWRhZGUgRmVkZXJhbCBkbyBSaW8gR3JhbmRlIGRvIE5vcnRlIG8gZGlyZWl0byBuw6NvLWV4Y2x1c2l2byBkZQpyZXByb2R1emlyLCBjb252ZXJ0ZXIgKGNvbW8gZGVmaW5pZG8gYWJhaXhvKSwgY29tdW5pY2FyIGUvb3UKZGlzdHJpYnVpciBvIGRvY3VtZW50byBlbnRyZWd1ZSAoaW5jbHVpbmRvIG8gcmVzdW1vL2Fic3RyYWN0KSBlbQpmb3JtYXRvIGRpZ2l0YWwgb3UgaW1wcmVzc28gZSBlbSBxdWFscXVlciBtZWlvLgoKYikgRGVjbGFyYSBxdWUgbyBkb2N1bWVudG8gZW50cmVndWUgw6kgc2V1IHRyYWJhbGhvIG9yaWdpbmFsLCBlIHF1ZQpkZXTDqW0gbyBkaXJlaXRvIGRlIGNvbmNlZGVyIG9zIGRpcmVpdG9zIGNvbnRpZG9zIG5lc3RhwqBsaWNlbsOnYS4gRGVjbGFyYQp0YW1iw6ltIHF1ZSBhIGVudHJlZ2EgZG8gZG9jdW1lbnRvIG7Do28gaW5mcmluZ2UsIHRhbnRvIHF1YW50byBsaGUgw6kKcG9zc8OtdmVsIHNhYmVyLCBvcyBkaXJlaXRvcyBkZSBxdWFscXVlciBvdXRyYSBwZXNzb2Egb3UgZW50aWRhZGUuCgpjKSBTZSBvIGRvY3VtZW50byBlbnRyZWd1ZSBjb250w6ltIG1hdGVyaWFsIGRvIHF1YWwgbsOjbyBkZXTDqW0gb3MKZGlyZWl0b3MgZGUgYXV0b3IsIGRlY2xhcmEgcXVlIG9idGV2ZSBhdXRvcml6YcOnw6NvIGRvIGRldGVudG9yIGRvcwpkaXJlaXRvcyBkZSBhdXRvciBwYXJhIGNvbmNlZGVyIMOgIFVuaXZlcnNpZGFkZSBGZWRlcmFsIGRvIFJpbyBHcmFuZGUgZG8gTm9ydGUgb3MgZGlyZWl0b3MgcmVxdWVyaWRvcyBwb3IgZXN0YcKgbGljZW7Dp2EsIGUgcXVlIGVzc2UgbWF0ZXJpYWwgY3Vqb3MgZGlyZWl0b3Mgc8OjbyBkZQp0ZXJjZWlyb3MgZXN0w6EgY2xhcmFtZW50ZSBpZGVudGlmaWNhZG8gZSByZWNvbmhlY2lkbyBubyB0ZXh0byBvdQpjb250ZcO6ZG8gZG8gZG9jdW1lbnRvIGVudHJlZ3VlLgoKU2UgbyBkb2N1bWVudG8gZW50cmVndWUgw6kgYmFzZWFkbyBlbSB0cmFiYWxobyBmaW5hbmNpYWRvIG91IGFwb2lhZG8KcG9yIG91dHJhIGluc3RpdHVpw6fDo28gcXVlIG7Do28gYSBVbml2ZXJzaWRhZGUgRmVkZXJhbCBkbyBSaW8gR3JhbmRlIGRvIE5vcnRlLCBkZWNsYXJhIHF1ZSBjdW1wcml1IHF1YWlzcXVlciBvYnJpZ2HDp8O1ZXMgZXhpZ2lkYXMgcGVsbyByZXNwZWN0aXZvIGNvbnRyYXRvIG91IGFjb3Jkby4KCkEgVW5pdmVyc2lkYWRlIEZlZGVyYWwgZG8gUmlvIEdyYW5kZSBkbyBOb3J0ZSBpZGVudGlmaWNhcsOhIGNsYXJhbWVudGUgbyhzKSBzZXUgKHMpIG5vbWUocykgY29tbyBvIChzKSBhdXRvciAoZXMpIG91IGRldGVudG9yIChlcykgZG9zIGRpcmVpdG9zIGRvIGRvY3VtZW50bwplbnRyZWd1ZSwgZSBuw6NvIGZhcsOhIHF1YWxxdWVyIGFsdGVyYcOnw6NvLCBwYXJhIGFsw6ltIGRhcyBwZXJtaXRpZGFzIHBvcgplc3RhwqBsaWNlbsOnYS4KRepositório de PublicaçõesPUBhttp://repositorio.ufrn.br/oai/opendoar:2024-03-19T04:06:10Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)false
dc.title.pt_BR.fl_str_mv Immobilization of lipases via interfacial activation on hydrophobic supports: Production of biocatalysts libraries by altering the immobilization conditions
title Immobilization of lipases via interfacial activation on hydrophobic supports: Production of biocatalysts libraries by altering the immobilization conditions
spellingShingle Immobilization of lipases via interfacial activation on hydrophobic supports: Production of biocatalysts libraries by altering the immobilization conditions
Peña, Sara Arana
Lipase modulation
Lipase stability
Interfacial activation
Lipase immobilization
Lipase specificity
title_short Immobilization of lipases via interfacial activation on hydrophobic supports: Production of biocatalysts libraries by altering the immobilization conditions
title_full Immobilization of lipases via interfacial activation on hydrophobic supports: Production of biocatalysts libraries by altering the immobilization conditions
title_fullStr Immobilization of lipases via interfacial activation on hydrophobic supports: Production of biocatalysts libraries by altering the immobilization conditions
title_full_unstemmed Immobilization of lipases via interfacial activation on hydrophobic supports: Production of biocatalysts libraries by altering the immobilization conditions
title_sort Immobilization of lipases via interfacial activation on hydrophobic supports: Production of biocatalysts libraries by altering the immobilization conditions
author Peña, Sara Arana
author_facet Peña, Sara Arana
Rios, Nathalia Saraiva
Carballares, Diego
Gonçalves, Luciana Rocha Barros
Lafuente, Roberto Fernandez
author_role author
author2 Rios, Nathalia Saraiva
Carballares, Diego
Gonçalves, Luciana Rocha Barros
Lafuente, Roberto Fernandez
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Peña, Sara Arana
Rios, Nathalia Saraiva
Carballares, Diego
Gonçalves, Luciana Rocha Barros
Lafuente, Roberto Fernandez
dc.subject.por.fl_str_mv Lipase modulation
Lipase stability
Interfacial activation
Lipase immobilization
Lipase specificity
topic Lipase modulation
Lipase stability
Interfacial activation
Lipase immobilization
Lipase specificity
description 2030-12
publishDate 2021
dc.date.accessioned.fl_str_mv 2021-11-29T21:23:24Z
dc.date.issued.fl_str_mv 2021-02-15
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv ARANA-PEÑA, Sara; RIOS, Nathalia S.; CARBALLARES, Diego; GONÇALVES, Luciana R.B.; FERNANDEZ-LAFUENTE, Roberto. Immobilization of lipases via interfacial activation on hydrophobic supports: production of biocatalysts libraries by altering the immobilization conditions. Catalysis Today, [S.L.], v. 362, p. 130-140, fev. 2021. Disponível em: https://www.sciencedirect.com/science/article/abs/pii/S0920586120301814?via%3Dihub#!. Acesso em: 29 nov. 2021. https://doi.org/10.1016/j.cattod.2020.03.059
dc.identifier.uri.fl_str_mv https://repositorio.ufrn.br/handle/123456789/45069
dc.identifier.issn.none.fl_str_mv 0920-5861
dc.identifier.doi.none.fl_str_mv 10.1016/j.cattod.2020.03.059
identifier_str_mv ARANA-PEÑA, Sara; RIOS, Nathalia S.; CARBALLARES, Diego; GONÇALVES, Luciana R.B.; FERNANDEZ-LAFUENTE, Roberto. Immobilization of lipases via interfacial activation on hydrophobic supports: production of biocatalysts libraries by altering the immobilization conditions. Catalysis Today, [S.L.], v. 362, p. 130-140, fev. 2021. Disponível em: https://www.sciencedirect.com/science/article/abs/pii/S0920586120301814?via%3Dihub#!. Acesso em: 29 nov. 2021. https://doi.org/10.1016/j.cattod.2020.03.059
0920-5861
10.1016/j.cattod.2020.03.059
url https://repositorio.ufrn.br/handle/123456789/45069
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFRN
instname:Universidade Federal do Rio Grande do Norte (UFRN)
instacron:UFRN
instname_str Universidade Federal do Rio Grande do Norte (UFRN)
instacron_str UFRN
institution UFRN
reponame_str Repositório Institucional da UFRN
collection Repositório Institucional da UFRN
bitstream.url.fl_str_mv https://repositorio.ufrn.br/bitstream/123456789/45069/2/license_rdf
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