Evaluation of styrene-divinylbenzene beads as a support to immobilize lipases

Detalhes bibliográficos
Autor(a) principal: Garcia-Galan, Cristina
Data de Publicação: 2014
Outros Autores: Barbosa, Oveimar, Hernandez, Karel, Santos, José C. S. dos, Rodrigues, Rafael Costa, Fernandez-Lafuente, Roberto
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFRGS
Texto Completo: http://hdl.handle.net/10183/267597
Resumo: A commercial and very hydrophobic styrene-divinylbenzene matrix, MCI GEL® CHP20P, has been compared to octyl-Sepharose® beads as support to immobilize three different enzymes: lipases from Thermomyces lanuginosus (TLL) and from Rhizomucor miehie (RML) and Lecitase® Ultra, a commercial artificial phospholipase. The immobilization mechanism on both supports was similar: interfacial activation of the enzymes versus the hydrophobic surface of the supports. Immobilization rate and loading capacity is much higher using MCI GEL® CHP20P compared to octyl-Sepharose® (87.2 mg protein/g of support using TLL, 310 mg/g using RML and 180 mg/g using Lecitase® Ultra). The thermal stability of all new preparations is much lower than that of the standard octyl-Sepharose® immobilized preparations, while the opposite occurs when the inactivations were performed in the presence of organic co-solvents. Regarding the hydrolytic activities, the results were strongly dependent on the substrate and pH of measurement. Octyl-Sepharose® immobilized enzymes were more active versus p-NPB than the enzymes immobilized on MCI GEL® CHP20P, while RML became 700-fold less active versus methyl phenylacetate. Thus, the immobilization of a lipase on this matrix needs to be empirically evaluated, since it may present very positive effects in some cases while in other cases it may have very negative ones.
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spelling Garcia-Galan, CristinaBarbosa, OveimarHernandez, KarelSantos, José C. S. dosRodrigues, Rafael CostaFernandez-Lafuente, Roberto2023-11-25T03:25:46Z20141420-3049http://hdl.handle.net/10183/267597000987533A commercial and very hydrophobic styrene-divinylbenzene matrix, MCI GEL® CHP20P, has been compared to octyl-Sepharose® beads as support to immobilize three different enzymes: lipases from Thermomyces lanuginosus (TLL) and from Rhizomucor miehie (RML) and Lecitase® Ultra, a commercial artificial phospholipase. The immobilization mechanism on both supports was similar: interfacial activation of the enzymes versus the hydrophobic surface of the supports. Immobilization rate and loading capacity is much higher using MCI GEL® CHP20P compared to octyl-Sepharose® (87.2 mg protein/g of support using TLL, 310 mg/g using RML and 180 mg/g using Lecitase® Ultra). The thermal stability of all new preparations is much lower than that of the standard octyl-Sepharose® immobilized preparations, while the opposite occurs when the inactivations were performed in the presence of organic co-solvents. Regarding the hydrolytic activities, the results were strongly dependent on the substrate and pH of measurement. Octyl-Sepharose® immobilized enzymes were more active versus p-NPB than the enzymes immobilized on MCI GEL® CHP20P, while RML became 700-fold less active versus methyl phenylacetate. Thus, the immobilization of a lipase on this matrix needs to be empirically evaluated, since it may present very positive effects in some cases while in other cases it may have very negative ones.application/pdfengMolecules. Basel, Switzerland. Vol. 19, no. 6 (June 2014), p. 7629-7645LipaseImobilização de lipasesLipase immobilizationModulation of lipase activityInterfacial activationStyrene divinylbencene matrixEvaluation of styrene-divinylbenzene beads as a support to immobilize lipasesEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT000987533.pdf.txt000987533.pdf.txtExtracted Texttext/plain47919http://www.lume.ufrgs.br/bitstream/10183/267597/2/000987533.pdf.txt0a9ef839f0207586be2ba7b7e4a27032MD52ORIGINAL000987533.pdfTexto completo (inglês)application/pdf245764http://www.lume.ufrgs.br/bitstream/10183/267597/1/000987533.pdf383b588b54df9db6ca3feee7b76725c5MD5110183/2675972023-12-06 04:24:19.830006oai:www.lume.ufrgs.br:10183/267597Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2023-12-06T06:24:19Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false
dc.title.pt_BR.fl_str_mv Evaluation of styrene-divinylbenzene beads as a support to immobilize lipases
title Evaluation of styrene-divinylbenzene beads as a support to immobilize lipases
spellingShingle Evaluation of styrene-divinylbenzene beads as a support to immobilize lipases
Garcia-Galan, Cristina
Lipase
Imobilização de lipases
Lipase immobilization
Modulation of lipase activity
Interfacial activation
Styrene divinylbencene matrix
title_short Evaluation of styrene-divinylbenzene beads as a support to immobilize lipases
title_full Evaluation of styrene-divinylbenzene beads as a support to immobilize lipases
title_fullStr Evaluation of styrene-divinylbenzene beads as a support to immobilize lipases
title_full_unstemmed Evaluation of styrene-divinylbenzene beads as a support to immobilize lipases
title_sort Evaluation of styrene-divinylbenzene beads as a support to immobilize lipases
author Garcia-Galan, Cristina
author_facet Garcia-Galan, Cristina
Barbosa, Oveimar
Hernandez, Karel
Santos, José C. S. dos
Rodrigues, Rafael Costa
Fernandez-Lafuente, Roberto
author_role author
author2 Barbosa, Oveimar
Hernandez, Karel
Santos, José C. S. dos
Rodrigues, Rafael Costa
Fernandez-Lafuente, Roberto
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Garcia-Galan, Cristina
Barbosa, Oveimar
Hernandez, Karel
Santos, José C. S. dos
Rodrigues, Rafael Costa
Fernandez-Lafuente, Roberto
dc.subject.por.fl_str_mv Lipase
Imobilização de lipases
topic Lipase
Imobilização de lipases
Lipase immobilization
Modulation of lipase activity
Interfacial activation
Styrene divinylbencene matrix
dc.subject.eng.fl_str_mv Lipase immobilization
Modulation of lipase activity
Interfacial activation
Styrene divinylbencene matrix
description A commercial and very hydrophobic styrene-divinylbenzene matrix, MCI GEL® CHP20P, has been compared to octyl-Sepharose® beads as support to immobilize three different enzymes: lipases from Thermomyces lanuginosus (TLL) and from Rhizomucor miehie (RML) and Lecitase® Ultra, a commercial artificial phospholipase. The immobilization mechanism on both supports was similar: interfacial activation of the enzymes versus the hydrophobic surface of the supports. Immobilization rate and loading capacity is much higher using MCI GEL® CHP20P compared to octyl-Sepharose® (87.2 mg protein/g of support using TLL, 310 mg/g using RML and 180 mg/g using Lecitase® Ultra). The thermal stability of all new preparations is much lower than that of the standard octyl-Sepharose® immobilized preparations, while the opposite occurs when the inactivations were performed in the presence of organic co-solvents. Regarding the hydrolytic activities, the results were strongly dependent on the substrate and pH of measurement. Octyl-Sepharose® immobilized enzymes were more active versus p-NPB than the enzymes immobilized on MCI GEL® CHP20P, while RML became 700-fold less active versus methyl phenylacetate. Thus, the immobilization of a lipase on this matrix needs to be empirically evaluated, since it may present very positive effects in some cases while in other cases it may have very negative ones.
publishDate 2014
dc.date.issued.fl_str_mv 2014
dc.date.accessioned.fl_str_mv 2023-11-25T03:25:46Z
dc.type.driver.fl_str_mv Estrangeiro
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dc.identifier.issn.pt_BR.fl_str_mv 1420-3049
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dc.relation.ispartof.pt_BR.fl_str_mv Molecules. Basel, Switzerland. Vol. 19, no. 6 (June 2014), p. 7629-7645
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