Evaluation of styrene-divinylbenzene beads as a support to immobilize lipases
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/267597 |
Resumo: | A commercial and very hydrophobic styrene-divinylbenzene matrix, MCI GEL® CHP20P, has been compared to octyl-Sepharose® beads as support to immobilize three different enzymes: lipases from Thermomyces lanuginosus (TLL) and from Rhizomucor miehie (RML) and Lecitase® Ultra, a commercial artificial phospholipase. The immobilization mechanism on both supports was similar: interfacial activation of the enzymes versus the hydrophobic surface of the supports. Immobilization rate and loading capacity is much higher using MCI GEL® CHP20P compared to octyl-Sepharose® (87.2 mg protein/g of support using TLL, 310 mg/g using RML and 180 mg/g using Lecitase® Ultra). The thermal stability of all new preparations is much lower than that of the standard octyl-Sepharose® immobilized preparations, while the opposite occurs when the inactivations were performed in the presence of organic co-solvents. Regarding the hydrolytic activities, the results were strongly dependent on the substrate and pH of measurement. Octyl-Sepharose® immobilized enzymes were more active versus p-NPB than the enzymes immobilized on MCI GEL® CHP20P, while RML became 700-fold less active versus methyl phenylacetate. Thus, the immobilization of a lipase on this matrix needs to be empirically evaluated, since it may present very positive effects in some cases while in other cases it may have very negative ones. |
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Garcia-Galan, CristinaBarbosa, OveimarHernandez, KarelSantos, José C. S. dosRodrigues, Rafael CostaFernandez-Lafuente, Roberto2023-11-25T03:25:46Z20141420-3049http://hdl.handle.net/10183/267597000987533A commercial and very hydrophobic styrene-divinylbenzene matrix, MCI GEL® CHP20P, has been compared to octyl-Sepharose® beads as support to immobilize three different enzymes: lipases from Thermomyces lanuginosus (TLL) and from Rhizomucor miehie (RML) and Lecitase® Ultra, a commercial artificial phospholipase. The immobilization mechanism on both supports was similar: interfacial activation of the enzymes versus the hydrophobic surface of the supports. Immobilization rate and loading capacity is much higher using MCI GEL® CHP20P compared to octyl-Sepharose® (87.2 mg protein/g of support using TLL, 310 mg/g using RML and 180 mg/g using Lecitase® Ultra). The thermal stability of all new preparations is much lower than that of the standard octyl-Sepharose® immobilized preparations, while the opposite occurs when the inactivations were performed in the presence of organic co-solvents. Regarding the hydrolytic activities, the results were strongly dependent on the substrate and pH of measurement. Octyl-Sepharose® immobilized enzymes were more active versus p-NPB than the enzymes immobilized on MCI GEL® CHP20P, while RML became 700-fold less active versus methyl phenylacetate. Thus, the immobilization of a lipase on this matrix needs to be empirically evaluated, since it may present very positive effects in some cases while in other cases it may have very negative ones.application/pdfengMolecules. Basel, Switzerland. Vol. 19, no. 6 (June 2014), p. 7629-7645LipaseImobilização de lipasesLipase immobilizationModulation of lipase activityInterfacial activationStyrene divinylbencene matrixEvaluation of styrene-divinylbenzene beads as a support to immobilize lipasesEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT000987533.pdf.txt000987533.pdf.txtExtracted Texttext/plain47919http://www.lume.ufrgs.br/bitstream/10183/267597/2/000987533.pdf.txt0a9ef839f0207586be2ba7b7e4a27032MD52ORIGINAL000987533.pdfTexto completo (inglês)application/pdf245764http://www.lume.ufrgs.br/bitstream/10183/267597/1/000987533.pdf383b588b54df9db6ca3feee7b76725c5MD5110183/2675972023-12-06 04:24:19.830006oai:www.lume.ufrgs.br:10183/267597Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2023-12-06T06:24:19Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Evaluation of styrene-divinylbenzene beads as a support to immobilize lipases |
title |
Evaluation of styrene-divinylbenzene beads as a support to immobilize lipases |
spellingShingle |
Evaluation of styrene-divinylbenzene beads as a support to immobilize lipases Garcia-Galan, Cristina Lipase Imobilização de lipases Lipase immobilization Modulation of lipase activity Interfacial activation Styrene divinylbencene matrix |
title_short |
Evaluation of styrene-divinylbenzene beads as a support to immobilize lipases |
title_full |
Evaluation of styrene-divinylbenzene beads as a support to immobilize lipases |
title_fullStr |
Evaluation of styrene-divinylbenzene beads as a support to immobilize lipases |
title_full_unstemmed |
Evaluation of styrene-divinylbenzene beads as a support to immobilize lipases |
title_sort |
Evaluation of styrene-divinylbenzene beads as a support to immobilize lipases |
author |
Garcia-Galan, Cristina |
author_facet |
Garcia-Galan, Cristina Barbosa, Oveimar Hernandez, Karel Santos, José C. S. dos Rodrigues, Rafael Costa Fernandez-Lafuente, Roberto |
author_role |
author |
author2 |
Barbosa, Oveimar Hernandez, Karel Santos, José C. S. dos Rodrigues, Rafael Costa Fernandez-Lafuente, Roberto |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Garcia-Galan, Cristina Barbosa, Oveimar Hernandez, Karel Santos, José C. S. dos Rodrigues, Rafael Costa Fernandez-Lafuente, Roberto |
dc.subject.por.fl_str_mv |
Lipase Imobilização de lipases |
topic |
Lipase Imobilização de lipases Lipase immobilization Modulation of lipase activity Interfacial activation Styrene divinylbencene matrix |
dc.subject.eng.fl_str_mv |
Lipase immobilization Modulation of lipase activity Interfacial activation Styrene divinylbencene matrix |
description |
A commercial and very hydrophobic styrene-divinylbenzene matrix, MCI GEL® CHP20P, has been compared to octyl-Sepharose® beads as support to immobilize three different enzymes: lipases from Thermomyces lanuginosus (TLL) and from Rhizomucor miehie (RML) and Lecitase® Ultra, a commercial artificial phospholipase. The immobilization mechanism on both supports was similar: interfacial activation of the enzymes versus the hydrophobic surface of the supports. Immobilization rate and loading capacity is much higher using MCI GEL® CHP20P compared to octyl-Sepharose® (87.2 mg protein/g of support using TLL, 310 mg/g using RML and 180 mg/g using Lecitase® Ultra). The thermal stability of all new preparations is much lower than that of the standard octyl-Sepharose® immobilized preparations, while the opposite occurs when the inactivations were performed in the presence of organic co-solvents. Regarding the hydrolytic activities, the results were strongly dependent on the substrate and pH of measurement. Octyl-Sepharose® immobilized enzymes were more active versus p-NPB than the enzymes immobilized on MCI GEL® CHP20P, while RML became 700-fold less active versus methyl phenylacetate. Thus, the immobilization of a lipase on this matrix needs to be empirically evaluated, since it may present very positive effects in some cases while in other cases it may have very negative ones. |
publishDate |
2014 |
dc.date.issued.fl_str_mv |
2014 |
dc.date.accessioned.fl_str_mv |
2023-11-25T03:25:46Z |
dc.type.driver.fl_str_mv |
Estrangeiro info:eu-repo/semantics/article |
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info:eu-repo/semantics/publishedVersion |
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publishedVersion |
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http://hdl.handle.net/10183/267597 |
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1420-3049 |
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000987533 |
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http://hdl.handle.net/10183/267597 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Molecules. Basel, Switzerland. Vol. 19, no. 6 (June 2014), p. 7629-7645 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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