Perfil fenotípico de virulência e resistência à sulfadiazina da cepa atípica Ck1, de Toxoplasma gondii, isoladas no RN: estudo do comportamento cognitivo em roedores

• Autor(a) principal: Andrade, Joelma Maria de Araújo
• Data de Publicação: 2016
• Tipo de documento: Trabalho de conclusão de curso
• Idioma: por
• Título da fonte: Repositório Institucional da UFRN
• Texto Completo: https://repositorio.ufrn.br/handle/123456789/43251
• topic: Toxoplasma gondii; Toxoplasma gondii; Manipulação Comportamental; Behavior manipulation; Isolados atípicos; Atypical strains

Toxoplasma gondii (T. gondii) is a neurotrophic protozoan that causes toxoplasmosis, a highly worldwide prevalent disease. The parasite strains show high diversity within a few clonal lineages. However, in the last years, several authors detected atypical strains of T. gondii, especially in the southern hemisphere. In this study, we checked the virulence profile of atypical isolate of T. gondii TgCkBrRN1 (Ck1) obtained from chickens of extensive poultry production system in the Rio Grande do Norte, Brazil. We observed the virulence profile after inoculating five cysts from three different strains of T. gondii (VEG, ME49, and Ck1) into twenty-five male mice. We compare all infected groups with a saline control group (CRTL). For resistance protocol, we administered sulfadiazine for six days in all infected mice groups (treated - T), called of VEG-T, ME49-T, and Ck1-T groups. Instead, in the virulence protocol (nontreated - NT), we infected all twenty-five male mice with both three strains (VEG-NT, ME49-NT, and Ck1-NT) but any animal received treatment. For both protocols, we quantified the brain cysts and mortality. Anxiety-like and memory performance were assessed in plus-maze discriminative avoidance task. Our results demonstrated similar virulence profile between atypical strains with classical clonal strains. However, the behavioral performance of the atypical straininfected mice suggested a singular adaptation of T. gondii in the local definitive hosts, since this strain does not induce anxiety-like and memory impairment in the animals. Thus, it is possible to suggest that the atypical strain Ck1 has low virulence, low morbidity and do not modulate the behavior of their hosts. Although, more studies are necessary to make clear the mechanistic response of the atypical strains of T. gondii.