Modulação da apoptose em células BeWo infectadas por cepas atípicas (Udi1CH-05 e Udi2CH-05) de Toxoplasma gondii
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFU |
Texto Completo: | https://repositorio.ufu.br/handle/123456789/12388 https://doi.org/10.14393/ufu.di.2013.168 |
Resumo: | Toxoplasma gondii is an obligate intracellular parasite that presents different strain types and degrees of virulence. The strains are classificated like clonal or typical and exotic or atypical. This parasite presents several evasion mechanisms, which guarantees the successful replication in the host cell and, between these mechanisms, there is ability to modulate apoptosis of infected cells as well of cells in the microenvironment of infection. The present work aimed to evaluate de ability of two atypical strains of T. gondii (Udi1CH-05 and Udi2CH-05) on modulation of apoptosis in BeWo cells, the susceptibility of these cells by infection, as well as the influence of those strains on the standard of secretion of cytokines by the trophoblastic cells lineage. These parameters were evaluated from the perspective of the immediate origin of the strains derived directly from cell culture or from animals and maintained for a short period in culture (here called Calomys callosus / culture). The Udi1CH-05 culture category upregulated apoptosis from 12 hours post infection, and from 2 hours of infection by Udi2CH-05, culture category, apoptosis was uncontrolled in BeWo cells. The condition C. callosus / culture of both strains didn t control apoptosis when compared to uninfected control. BeWo cells infected with Udi2CH-05, C. callosus / culture category produce high levels of IL-12 in the early stages of infection, suggesting a typical inflammatory response. Levels of TNF-α were higher in the early stages of infection and with a gradual decrease production, coinciding with failure to induce apoptosis when cells were infected BeWo Udi2CH-05, C. callosus / culture category. The levels of IL-10 found during infection of BeWo cells by Udi1CH-05 indicate that other cytokines may be involved in the control of infection, whereas this cytokine acts in controlling the immune response induced by infection of cells by BeWo Udi2CH-05, C. callosus / culture category. The production of TGF-β detected by infection Udi1CH-05 C. callosus / culture or Udi2CH-05 of both categories, shows that this cytokine acts in the immunoregulation of infected cells, whereas infection by Udi1CH-05, culture category, suggests that other anti-inflammatory cytokines can participate in immunoregulation, since the increase the apoptosis rate coincided with the decreased production of TGF-β. |
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2016-06-22T18:31:49Z2013-07-312016-06-22T18:31:49Z2013-03-08MARIA, Janice Buiate Lopes. Modulação da apoptose em células BeWo infectadas por cepas atípicas (Udi1CH-05 e Udi2CH-05) de Toxoplasma gondii. 2013. 75 f. Dissertação (Mestrado em Ciências Biomédicas) - Universidade Federal de Uberlândia, Uberlândia, 2013. DOI https://doi.org/10.14393/ufu.di.2013.168https://repositorio.ufu.br/handle/123456789/12388https://doi.org/10.14393/ufu.di.2013.168Toxoplasma gondii is an obligate intracellular parasite that presents different strain types and degrees of virulence. The strains are classificated like clonal or typical and exotic or atypical. This parasite presents several evasion mechanisms, which guarantees the successful replication in the host cell and, between these mechanisms, there is ability to modulate apoptosis of infected cells as well of cells in the microenvironment of infection. The present work aimed to evaluate de ability of two atypical strains of T. gondii (Udi1CH-05 and Udi2CH-05) on modulation of apoptosis in BeWo cells, the susceptibility of these cells by infection, as well as the influence of those strains on the standard of secretion of cytokines by the trophoblastic cells lineage. These parameters were evaluated from the perspective of the immediate origin of the strains derived directly from cell culture or from animals and maintained for a short period in culture (here called Calomys callosus / culture). The Udi1CH-05 culture category upregulated apoptosis from 12 hours post infection, and from 2 hours of infection by Udi2CH-05, culture category, apoptosis was uncontrolled in BeWo cells. The condition C. callosus / culture of both strains didn t control apoptosis when compared to uninfected control. BeWo cells infected with Udi2CH-05, C. callosus / culture category produce high levels of IL-12 in the early stages of infection, suggesting a typical inflammatory response. Levels of TNF-α were higher in the early stages of infection and with a gradual decrease production, coinciding with failure to induce apoptosis when cells were infected BeWo Udi2CH-05, C. callosus / culture category. The levels of IL-10 found during infection of BeWo cells by Udi1CH-05 indicate that other cytokines may be involved in the control of infection, whereas this cytokine acts in controlling the immune response induced by infection of cells by BeWo Udi2CH-05, C. callosus / culture category. The production of TGF-β detected by infection Udi1CH-05 C. callosus / culture or Udi2CH-05 of both categories, shows that this cytokine acts in the immunoregulation of infected cells, whereas infection by Udi1CH-05, culture category, suggests that other anti-inflammatory cytokines can participate in immunoregulation, since the increase the apoptosis rate coincided with the decreased production of TGF-β.Toxoplasma gondii é um parasito intracelular obrigatório que apresenta diferentes tipos de cepas e graus variados de virulência. As cepas são classificadas como clonais e cepas exóticas ou atípicas. Este parasito apresenta diversos mecanismos de evasão, o que garante o sucesso na replicação na célula hospedeira e, entre estes mecanismos, destaca-se habilidade de modular a apoptose tanto de células infectadas quanto de células presentes no microambiente da infecção. O presente trabalho objetivou avaliar a habilidade de duas cepas atípicas de T. gondii (Udi1CH-05 e Udi2CH-05) em modular a apoptose de células BeWo, a susceptibilidade destas células a este parasito, bem como a influência de tais cepas no padrão de secreção de citocinas por esta linhagem de células trofoblásticas. Estes parâmetros foram avaliados sob a perspectiva da procedência imediata das cepas oriundas diretamente de cultura celular ou oriundas de animais e mantidas por curto período em cultura (aqui denominada Calomys callosus/cultura). A categoria cultura de Udi1CH-05 modulou positivamente a apoptose a partir de 12 horas de infecção, e a partir de 2 horas de infecção por Udi2CH-05, categoria cultura a apoptose não foi controlada em células BeWo. A condição C. callosus/cultura de ambas as cepas não controlou a apoptose quando comparado ao controle não infectado. As células BeWo infectadas com Udi2CH-05, categoria C. callosus/cultura produzem altos níveis de IL-12 nos estágios iniciais da infecção, sugerindo o desencadeamento de resposta inflamatória típica. Os níveis de TNF-α foram maiores nos estágios iniciais de infecção e com diminuição gradativa de produção, coincidindo com incapacidade em induzir a apoptose quando células BeWo foram infectadas por Udi2CH-05, categoria C. callosus/cultura. Os níveis de IL-10 encontrados durante a infecção de células BeWo por Udi1CH-05, indicam que outras citocinas podem estar envolvidas no controle da infecção, enquanto que essa citocina atua no controle da resposta imune desencadeada pela infecção de células BeWo por Udi2CH-05, categoria C. callosus/cultura. A produção de TGF-β detectada mediante a infecção por Udi1CH-05 C. callosus/cultura ou por Udi2CH-05 de ambas as categorias, demonstra que essa citocina atua na imunorregulação de células infectadas, enquanto que a infecção por Udi1CH-05, categoria cultura sugere que outras citocinas anti-inflamatórias possam participar da imunorregulação, pois o aumento do índice de apoptose coincidiu com a diminuição da produção de TGF-β.Mestre em Biologia Celular e Estrutural Aplicadasapplication/pdfporUniversidade Federal de UberlândiaPrograma de Pós-graduação em Biologia Celular e Estrutural AplicadasUFUBRCiências BiomédicasToxoplasma gondiiApoptoseCepas atípicasApoptosisAtypical strainsCNPQ::CIENCIAS BIOLOGICAS::MORFOLOGIA::CITOLOGIA E BIOLOGIA CELULARModulação da apoptose em células BeWo infectadas por cepas atípicas (Udi1CH-05 e Udi2CH-05) de Toxoplasma gondiiinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisMineo, Jose Robertohttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4780327P9Ferro, Eloisa Amália Vieirahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4784232Z5Barbosa, Bellisa de Freitashttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4736727J6Costa, Idessania Nazareth dahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4766013A0http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4274326Y5Maria, Janice Buiate Lopes81761369info:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFUinstname:Universidade Federal de Uberlândia (UFU)instacron:UFUTHUMBNAILJanice Buiate.pdf.jpgJanice Buiate.pdf.jpgGenerated Thumbnailimage/jpeg1155https://repositorio.ufu.br/bitstream/123456789/12388/3/Janice%20Buiate.pdf.jpg4d942d62df47d757f25f0c9a6442f84dMD53ORIGINALJanice Buiate.pdfapplication/pdf1874603https://repositorio.ufu.br/bitstream/123456789/12388/1/Janice%20Buiate.pdf1991b71efa8d504a6259ecbdc12003bdMD51TEXTJanice Buiate.pdf.txtJanice Buiate.pdf.txtExtracted texttext/plain160013https://repositorio.ufu.br/bitstream/123456789/12388/2/Janice%20Buiate.pdf.txt560adf00ed19e959d28de4fd01860a39MD52123456789/123882022-09-06 15:32:48.919oai:repositorio.ufu.br:123456789/12388Repositório InstitucionalONGhttp://repositorio.ufu.br/oai/requestdiinf@dirbi.ufu.bropendoar:2022-09-06T18:32:48Repositório Institucional da UFU - Universidade Federal de Uberlândia (UFU)false |
dc.title.por.fl_str_mv |
Modulação da apoptose em células BeWo infectadas por cepas atípicas (Udi1CH-05 e Udi2CH-05) de Toxoplasma gondii |
title |
Modulação da apoptose em células BeWo infectadas por cepas atípicas (Udi1CH-05 e Udi2CH-05) de Toxoplasma gondii |
spellingShingle |
Modulação da apoptose em células BeWo infectadas por cepas atípicas (Udi1CH-05 e Udi2CH-05) de Toxoplasma gondii Maria, Janice Buiate Lopes Toxoplasma gondii Apoptose Cepas atípicas Apoptosis Atypical strains CNPQ::CIENCIAS BIOLOGICAS::MORFOLOGIA::CITOLOGIA E BIOLOGIA CELULAR |
title_short |
Modulação da apoptose em células BeWo infectadas por cepas atípicas (Udi1CH-05 e Udi2CH-05) de Toxoplasma gondii |
title_full |
Modulação da apoptose em células BeWo infectadas por cepas atípicas (Udi1CH-05 e Udi2CH-05) de Toxoplasma gondii |
title_fullStr |
Modulação da apoptose em células BeWo infectadas por cepas atípicas (Udi1CH-05 e Udi2CH-05) de Toxoplasma gondii |
title_full_unstemmed |
Modulação da apoptose em células BeWo infectadas por cepas atípicas (Udi1CH-05 e Udi2CH-05) de Toxoplasma gondii |
title_sort |
Modulação da apoptose em células BeWo infectadas por cepas atípicas (Udi1CH-05 e Udi2CH-05) de Toxoplasma gondii |
author |
Maria, Janice Buiate Lopes |
author_facet |
Maria, Janice Buiate Lopes |
author_role |
author |
dc.contributor.advisor-co1.fl_str_mv |
Mineo, Jose Roberto |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4780327P9 |
dc.contributor.advisor1.fl_str_mv |
Ferro, Eloisa Amália Vieira |
dc.contributor.advisor1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4784232Z5 |
dc.contributor.referee1.fl_str_mv |
Barbosa, Bellisa de Freitas |
dc.contributor.referee1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4736727J6 |
dc.contributor.referee2.fl_str_mv |
Costa, Idessania Nazareth da |
dc.contributor.referee2Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4766013A0 |
dc.contributor.authorLattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4274326Y5 |
dc.contributor.author.fl_str_mv |
Maria, Janice Buiate Lopes |
contributor_str_mv |
Mineo, Jose Roberto Ferro, Eloisa Amália Vieira Barbosa, Bellisa de Freitas Costa, Idessania Nazareth da |
dc.subject.por.fl_str_mv |
Toxoplasma gondii Apoptose Cepas atípicas |
topic |
Toxoplasma gondii Apoptose Cepas atípicas Apoptosis Atypical strains CNPQ::CIENCIAS BIOLOGICAS::MORFOLOGIA::CITOLOGIA E BIOLOGIA CELULAR |
dc.subject.eng.fl_str_mv |
Apoptosis Atypical strains |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS BIOLOGICAS::MORFOLOGIA::CITOLOGIA E BIOLOGIA CELULAR |
description |
Toxoplasma gondii is an obligate intracellular parasite that presents different strain types and degrees of virulence. The strains are classificated like clonal or typical and exotic or atypical. This parasite presents several evasion mechanisms, which guarantees the successful replication in the host cell and, between these mechanisms, there is ability to modulate apoptosis of infected cells as well of cells in the microenvironment of infection. The present work aimed to evaluate de ability of two atypical strains of T. gondii (Udi1CH-05 and Udi2CH-05) on modulation of apoptosis in BeWo cells, the susceptibility of these cells by infection, as well as the influence of those strains on the standard of secretion of cytokines by the trophoblastic cells lineage. These parameters were evaluated from the perspective of the immediate origin of the strains derived directly from cell culture or from animals and maintained for a short period in culture (here called Calomys callosus / culture). The Udi1CH-05 culture category upregulated apoptosis from 12 hours post infection, and from 2 hours of infection by Udi2CH-05, culture category, apoptosis was uncontrolled in BeWo cells. The condition C. callosus / culture of both strains didn t control apoptosis when compared to uninfected control. BeWo cells infected with Udi2CH-05, C. callosus / culture category produce high levels of IL-12 in the early stages of infection, suggesting a typical inflammatory response. Levels of TNF-α were higher in the early stages of infection and with a gradual decrease production, coinciding with failure to induce apoptosis when cells were infected BeWo Udi2CH-05, C. callosus / culture category. The levels of IL-10 found during infection of BeWo cells by Udi1CH-05 indicate that other cytokines may be involved in the control of infection, whereas this cytokine acts in controlling the immune response induced by infection of cells by BeWo Udi2CH-05, C. callosus / culture category. The production of TGF-β detected by infection Udi1CH-05 C. callosus / culture or Udi2CH-05 of both categories, shows that this cytokine acts in the immunoregulation of infected cells, whereas infection by Udi1CH-05, culture category, suggests that other anti-inflammatory cytokines can participate in immunoregulation, since the increase the apoptosis rate coincided with the decreased production of TGF-β. |
publishDate |
2013 |
dc.date.available.fl_str_mv |
2013-07-31 2016-06-22T18:31:49Z |
dc.date.issued.fl_str_mv |
2013-03-08 |
dc.date.accessioned.fl_str_mv |
2016-06-22T18:31:49Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/masterThesis |
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masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
MARIA, Janice Buiate Lopes. Modulação da apoptose em células BeWo infectadas por cepas atípicas (Udi1CH-05 e Udi2CH-05) de Toxoplasma gondii. 2013. 75 f. Dissertação (Mestrado em Ciências Biomédicas) - Universidade Federal de Uberlândia, Uberlândia, 2013. DOI https://doi.org/10.14393/ufu.di.2013.168 |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufu.br/handle/123456789/12388 |
dc.identifier.doi.none.fl_str_mv |
https://doi.org/10.14393/ufu.di.2013.168 |
identifier_str_mv |
MARIA, Janice Buiate Lopes. Modulação da apoptose em células BeWo infectadas por cepas atípicas (Udi1CH-05 e Udi2CH-05) de Toxoplasma gondii. 2013. 75 f. Dissertação (Mestrado em Ciências Biomédicas) - Universidade Federal de Uberlândia, Uberlândia, 2013. DOI https://doi.org/10.14393/ufu.di.2013.168 |
url |
https://repositorio.ufu.br/handle/123456789/12388 https://doi.org/10.14393/ufu.di.2013.168 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Universidade Federal de Uberlândia |
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Programa de Pós-graduação em Biologia Celular e Estrutural Aplicadas |
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UFU |
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BR |
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Ciências Biomédicas |
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Universidade Federal de Uberlândia |
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