Production of recombinant 503 antigen of Leishmania infantum chagasi using cultivation in batch and fed-batch
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRN |
Texto Completo: | https://repositorio.ufrn.br/handle/123456789/32541 |
Resumo: | Visceral leishmaniasis is an infecto-parasitarian disease caused by obligatory intracellular protozoa belonging to the genus Leishmania, and might be lethal since there is no precocious diagnosis and proper treatment. Despite the considerable effort, there is no effective and safe vaccine for human use [1]. Genetically modified micro-organisms are used industrially in general for production of hormone, antibiotics and proteins. In the production of heterologous molecules, both the expression and the production step are important [2]. Therefore, the study of cultivation conditions in bioreactor plays an important role for the process viability of batch and fed-batch [3]. Thus, the aim of this work was to study strategies for production of the 503 antigen of Leishmania infantum chagasi using cultivation in batch and fed-batch |
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Santos, Everaldo Silvino dosVaz, Michelle Rossana FerreiraSousa Junior, Francisco Canindé dePadilha, Carlos Eduardo de AraújoMartins, Daniella Regina ArantesMacedo, Gorete Ribeiro2021-05-21T00:05:01Z2021-05-21T00:05:01Z2014-10-10VAZ, Michelle Rossana Ferreira; SOUSA JUNIOR, Francisco Canindé de; PADILHA, Carlos Eduardo de Araújo; MARTINS, Daniella Regina Arantes; SANTOS, Everaldo Silvino; MACEDO, Gorete Ribeiro. Production of recombinant 503 antigen of Leishmania infantum chagasi using cultivation in batch and fed-batch. Bmc Proceedings, [S.L.], v. 8, n. 4, out. 2014. Disponível em: https://bmcproc.biomedcentral.com/articles/10.1186/1753-6561-8-S4-P186. Acesso em: 08 abr 2021. http://dx.doi.org/10.1186/1753-6561-8-s4-p186.1753-6561https://repositorio.ufrn.br/handle/123456789/3254110.1186/1753-6561-8-S4-P186BMC ProceedingsAttribution 3.0 Brazilhttp://creativecommons.org/licenses/by/3.0/br/info:eu-repo/semantics/openAccessRecombinant ProteinLactoseVisceral LeishmaniasisHigh Cell DensityLeishmaniasisProduction of recombinant 503 antigen of Leishmania infantum chagasi using cultivation in batch and fed-batchinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleVisceral leishmaniasis is an infecto-parasitarian disease caused by obligatory intracellular protozoa belonging to the genus Leishmania, and might be lethal since there is no precocious diagnosis and proper treatment. Despite the considerable effort, there is no effective and safe vaccine for human use [1]. Genetically modified micro-organisms are used industrially in general for production of hormone, antibiotics and proteins. In the production of heterologous molecules, both the expression and the production step are important [2]. Therefore, the study of cultivation conditions in bioreactor plays an important role for the process viability of batch and fed-batch [3]. Thus, the aim of this work was to study strategies for production of the 503 antigen of Leishmania infantum chagasi using cultivation in batch and fed-batchengreponame:Repositório Institucional da UFRNinstname:Universidade Federal do Rio Grande do Norte (UFRN)instacron:UFRNORIGINALProductionRecombinant503_Santos_2014.pdfProductionRecombinant503_Santos_2014.pdfapplication/pdf240338https://repositorio.ufrn.br/bitstream/123456789/32541/1/ProductionRecombinant503_Santos_2014.pdfb0e03515366866b8dbb032a536405c6dMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81484https://repositorio.ufrn.br/bitstream/123456789/32541/3/license.txte9597aa2854d128fd968be5edc8a28d9MD53CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8914https://repositorio.ufrn.br/bitstream/123456789/32541/2/license_rdf4d2950bda3d176f570a9f8b328dfbbefMD52123456789/325412021-05-20 21:05:03.692oai:https://repositorio.ufrn.br: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Repositório de PublicaçõesPUBhttp://repositorio.ufrn.br/oai/opendoar:2021-05-21T00:05:03Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)false |
dc.title.pt_BR.fl_str_mv |
Production of recombinant 503 antigen of Leishmania infantum chagasi using cultivation in batch and fed-batch |
title |
Production of recombinant 503 antigen of Leishmania infantum chagasi using cultivation in batch and fed-batch |
spellingShingle |
Production of recombinant 503 antigen of Leishmania infantum chagasi using cultivation in batch and fed-batch Santos, Everaldo Silvino dos Recombinant Protein Lactose Visceral Leishmaniasis High Cell Density Leishmaniasis |
title_short |
Production of recombinant 503 antigen of Leishmania infantum chagasi using cultivation in batch and fed-batch |
title_full |
Production of recombinant 503 antigen of Leishmania infantum chagasi using cultivation in batch and fed-batch |
title_fullStr |
Production of recombinant 503 antigen of Leishmania infantum chagasi using cultivation in batch and fed-batch |
title_full_unstemmed |
Production of recombinant 503 antigen of Leishmania infantum chagasi using cultivation in batch and fed-batch |
title_sort |
Production of recombinant 503 antigen of Leishmania infantum chagasi using cultivation in batch and fed-batch |
author |
Santos, Everaldo Silvino dos |
author_facet |
Santos, Everaldo Silvino dos Vaz, Michelle Rossana Ferreira Sousa Junior, Francisco Canindé de Padilha, Carlos Eduardo de Araújo Martins, Daniella Regina Arantes Macedo, Gorete Ribeiro |
author_role |
author |
author2 |
Vaz, Michelle Rossana Ferreira Sousa Junior, Francisco Canindé de Padilha, Carlos Eduardo de Araújo Martins, Daniella Regina Arantes Macedo, Gorete Ribeiro |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Santos, Everaldo Silvino dos Vaz, Michelle Rossana Ferreira Sousa Junior, Francisco Canindé de Padilha, Carlos Eduardo de Araújo Martins, Daniella Regina Arantes Macedo, Gorete Ribeiro |
dc.subject.por.fl_str_mv |
Recombinant Protein Lactose Visceral Leishmaniasis High Cell Density Leishmaniasis |
topic |
Recombinant Protein Lactose Visceral Leishmaniasis High Cell Density Leishmaniasis |
description |
Visceral leishmaniasis is an infecto-parasitarian disease caused by obligatory intracellular protozoa belonging to the genus Leishmania, and might be lethal since there is no precocious diagnosis and proper treatment. Despite the considerable effort, there is no effective and safe vaccine for human use [1]. Genetically modified micro-organisms are used industrially in general for production of hormone, antibiotics and proteins. In the production of heterologous molecules, both the expression and the production step are important [2]. Therefore, the study of cultivation conditions in bioreactor plays an important role for the process viability of batch and fed-batch [3]. Thus, the aim of this work was to study strategies for production of the 503 antigen of Leishmania infantum chagasi using cultivation in batch and fed-batch |
publishDate |
2014 |
dc.date.issued.fl_str_mv |
2014-10-10 |
dc.date.accessioned.fl_str_mv |
2021-05-21T00:05:01Z |
dc.date.available.fl_str_mv |
2021-05-21T00:05:01Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
VAZ, Michelle Rossana Ferreira; SOUSA JUNIOR, Francisco Canindé de; PADILHA, Carlos Eduardo de Araújo; MARTINS, Daniella Regina Arantes; SANTOS, Everaldo Silvino; MACEDO, Gorete Ribeiro. Production of recombinant 503 antigen of Leishmania infantum chagasi using cultivation in batch and fed-batch. Bmc Proceedings, [S.L.], v. 8, n. 4, out. 2014. Disponível em: https://bmcproc.biomedcentral.com/articles/10.1186/1753-6561-8-S4-P186. Acesso em: 08 abr 2021. http://dx.doi.org/10.1186/1753-6561-8-s4-p186. |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufrn.br/handle/123456789/32541 |
dc.identifier.issn.none.fl_str_mv |
1753-6561 |
dc.identifier.doi.none.fl_str_mv |
10.1186/1753-6561-8-S4-P186 |
identifier_str_mv |
VAZ, Michelle Rossana Ferreira; SOUSA JUNIOR, Francisco Canindé de; PADILHA, Carlos Eduardo de Araújo; MARTINS, Daniella Regina Arantes; SANTOS, Everaldo Silvino; MACEDO, Gorete Ribeiro. Production of recombinant 503 antigen of Leishmania infantum chagasi using cultivation in batch and fed-batch. Bmc Proceedings, [S.L.], v. 8, n. 4, out. 2014. Disponível em: https://bmcproc.biomedcentral.com/articles/10.1186/1753-6561-8-S4-P186. Acesso em: 08 abr 2021. http://dx.doi.org/10.1186/1753-6561-8-s4-p186. 1753-6561 10.1186/1753-6561-8-S4-P186 |
url |
https://repositorio.ufrn.br/handle/123456789/32541 |
dc.language.iso.fl_str_mv |
eng |
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eng |
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Attribution 3.0 Brazil http://creativecommons.org/licenses/by/3.0/br/ info:eu-repo/semantics/openAccess |
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Attribution 3.0 Brazil http://creativecommons.org/licenses/by/3.0/br/ |
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openAccess |
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BMC Proceedings |
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BMC Proceedings |
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