A low-molecular-weight galactofucan from the seaweed, Spatoglossum schröederi, binds fibronectin and inhibits capillary-like tube formation in vitro

• Autor(a) principal: Menezes, Maira Maria
• Data de Publicação: 2018
• Outros Autores: Nobre, Leonardo Thiago Duarte Barreto, Rossi, Gustavo Rodrigues, Almeida-Lima, Jailma, Melo-Silveira, Raniere Fagundes, Franco, Celia Regina Cavichiolo, Trindade, Edvaldo Silva, Nader, Helena Bonciani, Rocha, Hugo Alexandre de Oliveira
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UFRN
• Texto Completo: https://repositorio.ufrn.br/handle/123456789/31159
• topic: Fucoidan; Brown seaweed, endothelial cells; Anti-angiogenic

A low-molecular-weight (LMW) heterofucan (designated fucan B) was obtained from the brown seaweed, Spatoglossum schröederi, and its activity as an inhibitor of capillary-like tube formation by endothelial cells (ECs) was analyzed. Chemical, infrared and electrophoretic analyses confirmed the identity of fucan B. In contrast to other LMW fucans, fucan B (0.012–0.1 mg/mL) inhibited ECs capillary-like tube formation in a concentrationdependent manner. In addition, fucan B (0.01–0.05 mg/mL) did not affect ECs proliferation. Fucan B also inhibited ECs migration on a fibronectin-coated surface, but not on laminin- or collagen-coated surfaces. Biotinylated fucan B was used as a probe to identify its localization. Confocal microscopy experiments revealed that biotinylated fucan did not bind to the cell surface, but rather only to fibronectin. Our findings suggest that fucan B inhibits ECs capillary-like tube formation and migration by binding directly to fibronectin and blocking fibronectin sites recognized by cell surface ligands. However, further studies are needed to evaluate the in vivo effects of fucan B