A fucan from the brown seaweed Spatoglossum schröederi inhibits Chinese hamster ovary cell adhesion to several extracellular matrix proteins
Autor(a) principal: | |
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Data de Publicação: | 2001 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://dx.doi.org/10.1590/S0100-879X2001000500009 http://repositorio.unifesp.br/handle/11600/1159 |
Resumo: | Fucans, a family of sulfated polysaccharides present in brown seaweed, have several biological activities. Their use as drugs would offer the advantage of no potential risk of contamination with viruses or particles such as prions. A fucan prepared from Spatoglossum schröederi was tested as a possible inhibitor of cell-matrix interactions using wild-type Chinese hamster ovary cells (CHO-K1) and the mutant type deficient in xylosyltransferase (CHO-745). The effect of this polymer on adhesion properties with specific extracellular matrix components was studied using several matrix proteins as substrates for cell attachment. Treatment with the polymer inhibited the adhesion of fibronectin to both CHO-K1 (2 x 10(5))()and CHO-745 (2 x 10(5) and 5 x 10(5)) cells. No effect was detected with laminin, using the two cell types. On the other hand, adhesion to vitronectin was inhibited in CHO-K1 cells and adhesion to type I collagen was inhibited in CHO-745 cells. In spite of this inhibition, the fucan did not affect either cell proliferation or cell cycle. These results demonstrate that this polymer is a new anti-adhesive compound with potential pharmacological applications. |
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A fucan from the brown seaweed Spatoglossum schröederi inhibits Chinese hamster ovary cell adhesion to several extracellular matrix proteinsCHO cellsextracellular matrix proteinsfucanglycosaminoglycanscell adhesionbrown algaeFucans, a family of sulfated polysaccharides present in brown seaweed, have several biological activities. Their use as drugs would offer the advantage of no potential risk of contamination with viruses or particles such as prions. A fucan prepared from Spatoglossum schröederi was tested as a possible inhibitor of cell-matrix interactions using wild-type Chinese hamster ovary cells (CHO-K1) and the mutant type deficient in xylosyltransferase (CHO-745). The effect of this polymer on adhesion properties with specific extracellular matrix components was studied using several matrix proteins as substrates for cell attachment. Treatment with the polymer inhibited the adhesion of fibronectin to both CHO-K1 (2 x 10(5))()and CHO-745 (2 x 10(5) and 5 x 10(5)) cells. No effect was detected with laminin, using the two cell types. On the other hand, adhesion to vitronectin was inhibited in CHO-K1 cells and adhesion to type I collagen was inhibited in CHO-745 cells. In spite of this inhibition, the fucan did not affect either cell proliferation or cell cycle. These results demonstrate that this polymer is a new anti-adhesive compound with potential pharmacological applications.Universidade Federal do Rio Grande do Norte Departamento de BioquímicaUniversidade Federal do Paraná Departamento de Biologia CelularUniversidade Federal de São Paulo (UNIFESP) Escola Paulista de Medicina Departamento de BioquímicaUNIFESP, EPM, Depto. de BioquímicaSciELOAssociação Brasileira de Divulgação CientíficaUniversidade Federal do Rio Grande do Norte Departamento de BioquímicaUniversidade Federal do Paraná Departamento de Biologia CelularUniversidade Federal de São Paulo (UNIFESP)Rocha, Hugo Alexandre Oliveira [UNIFESP]Franco, Celia Regina Cavichlolo [UNIFESP]Trindade, Edvaldo da Silva [UNIFESP]Carvalho, L.c.m.Veiga, Silvio Sanches [UNIFESP]Leite, Edda Lisboa [UNIFESP]Dietrich, Carl Peter [UNIFESP]Nader, Helena Bonciani [UNIFESP]2015-06-14T13:29:23Z2015-06-14T13:29:23Z2001-05-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion621-626application/pdfhttp://dx.doi.org/10.1590/S0100-879X2001000500009Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 34, n. 5, p. 621-626, 2001.10.1590/S0100-879X2001000500009S0100-879X2001000500009.pdf0100-879XS0100-879X2001000500009http://repositorio.unifesp.br/handle/11600/1159WOS:000168735900009engBrazilian Journal of Medical and Biological Researchinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-05T22:46:12Zoai:repositorio.unifesp.br/:11600/1159Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-05T22:46:12Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.none.fl_str_mv |
A fucan from the brown seaweed Spatoglossum schröederi inhibits Chinese hamster ovary cell adhesion to several extracellular matrix proteins |
title |
A fucan from the brown seaweed Spatoglossum schröederi inhibits Chinese hamster ovary cell adhesion to several extracellular matrix proteins |
spellingShingle |
A fucan from the brown seaweed Spatoglossum schröederi inhibits Chinese hamster ovary cell adhesion to several extracellular matrix proteins Rocha, Hugo Alexandre Oliveira [UNIFESP] CHO cells extracellular matrix proteins fucan glycosaminoglycans cell adhesion brown algae |
title_short |
A fucan from the brown seaweed Spatoglossum schröederi inhibits Chinese hamster ovary cell adhesion to several extracellular matrix proteins |
title_full |
A fucan from the brown seaweed Spatoglossum schröederi inhibits Chinese hamster ovary cell adhesion to several extracellular matrix proteins |
title_fullStr |
A fucan from the brown seaweed Spatoglossum schröederi inhibits Chinese hamster ovary cell adhesion to several extracellular matrix proteins |
title_full_unstemmed |
A fucan from the brown seaweed Spatoglossum schröederi inhibits Chinese hamster ovary cell adhesion to several extracellular matrix proteins |
title_sort |
A fucan from the brown seaweed Spatoglossum schröederi inhibits Chinese hamster ovary cell adhesion to several extracellular matrix proteins |
author |
Rocha, Hugo Alexandre Oliveira [UNIFESP] |
author_facet |
Rocha, Hugo Alexandre Oliveira [UNIFESP] Franco, Celia Regina Cavichlolo [UNIFESP] Trindade, Edvaldo da Silva [UNIFESP] Carvalho, L.c.m. Veiga, Silvio Sanches [UNIFESP] Leite, Edda Lisboa [UNIFESP] Dietrich, Carl Peter [UNIFESP] Nader, Helena Bonciani [UNIFESP] |
author_role |
author |
author2 |
Franco, Celia Regina Cavichlolo [UNIFESP] Trindade, Edvaldo da Silva [UNIFESP] Carvalho, L.c.m. Veiga, Silvio Sanches [UNIFESP] Leite, Edda Lisboa [UNIFESP] Dietrich, Carl Peter [UNIFESP] Nader, Helena Bonciani [UNIFESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal do Rio Grande do Norte Departamento de Bioquímica Universidade Federal do Paraná Departamento de Biologia Celular Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Rocha, Hugo Alexandre Oliveira [UNIFESP] Franco, Celia Regina Cavichlolo [UNIFESP] Trindade, Edvaldo da Silva [UNIFESP] Carvalho, L.c.m. Veiga, Silvio Sanches [UNIFESP] Leite, Edda Lisboa [UNIFESP] Dietrich, Carl Peter [UNIFESP] Nader, Helena Bonciani [UNIFESP] |
dc.subject.por.fl_str_mv |
CHO cells extracellular matrix proteins fucan glycosaminoglycans cell adhesion brown algae |
topic |
CHO cells extracellular matrix proteins fucan glycosaminoglycans cell adhesion brown algae |
description |
Fucans, a family of sulfated polysaccharides present in brown seaweed, have several biological activities. Their use as drugs would offer the advantage of no potential risk of contamination with viruses or particles such as prions. A fucan prepared from Spatoglossum schröederi was tested as a possible inhibitor of cell-matrix interactions using wild-type Chinese hamster ovary cells (CHO-K1) and the mutant type deficient in xylosyltransferase (CHO-745). The effect of this polymer on adhesion properties with specific extracellular matrix components was studied using several matrix proteins as substrates for cell attachment. Treatment with the polymer inhibited the adhesion of fibronectin to both CHO-K1 (2 x 10(5))()and CHO-745 (2 x 10(5) and 5 x 10(5)) cells. No effect was detected with laminin, using the two cell types. On the other hand, adhesion to vitronectin was inhibited in CHO-K1 cells and adhesion to type I collagen was inhibited in CHO-745 cells. In spite of this inhibition, the fucan did not affect either cell proliferation or cell cycle. These results demonstrate that this polymer is a new anti-adhesive compound with potential pharmacological applications. |
publishDate |
2001 |
dc.date.none.fl_str_mv |
2001-05-01 2015-06-14T13:29:23Z 2015-06-14T13:29:23Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S0100-879X2001000500009 Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 34, n. 5, p. 621-626, 2001. 10.1590/S0100-879X2001000500009 S0100-879X2001000500009.pdf 0100-879X S0100-879X2001000500009 http://repositorio.unifesp.br/handle/11600/1159 WOS:000168735900009 |
url |
http://dx.doi.org/10.1590/S0100-879X2001000500009 http://repositorio.unifesp.br/handle/11600/1159 |
identifier_str_mv |
Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 34, n. 5, p. 621-626, 2001. 10.1590/S0100-879X2001000500009 S0100-879X2001000500009.pdf 0100-879X S0100-879X2001000500009 WOS:000168735900009 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Journal of Medical and Biological Research |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
621-626 application/pdf |
dc.publisher.none.fl_str_mv |
Associação Brasileira de Divulgação Científica |
publisher.none.fl_str_mv |
Associação Brasileira de Divulgação Científica |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
_version_ |
1814268384029704192 |