Coimmobilization of different lipases: Simple layer by layer enzyme spatial ordering

Detalhes bibliográficos
Autor(a) principal: Peña, Sara Arana
Data de Publicação: 2020
Outros Autores: Rios, Nathalia Saraiva, Sanchez, Carmen Mendez, Lokha, Yuliya, Carballares, Diego, Gonçalves, Luciana Rocha Barros, Lafuente, Roberto Fernandez
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFRN
Texto Completo: https://repositorio.ufrn.br/handle/123456789/45068
Resumo: 2030-12
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spelling Peña, Sara AranaRios, Nathalia SaraivaSanchez, Carmen MendezLokha, YuliyaCarballares, DiegoGonçalves, Luciana Rocha BarrosLafuente, Roberto Fernandez2021-11-29T21:16:03Z2020-02-15ARANA-PEÑA, Sara; RIOS, Nathalia S.; MENDEZ-SANCHEZ, Carmen; LOKHA, Yuliya; CARBALLARES, Diego; GONÇALVES, Luciana R.B.; FERNANDEZ-LAFUENTE, Roberto. Coimmobilization of different lipases: simple layer by layer enzyme spatial ordering. International Journal Of Biological Macromolecules, [S.L.], v. 145, p. 856-864, fev. 2020. Disponível em: https://www.sciencedirect.com/science/article/abs/pii/S0141813019371466?via%3Dihub#!. Acesso em: 29 nov. 2021. https://doi.org/10.1016/j.ijbiomac.2019.10.087.0141-8130https://repositorio.ufrn.br/handle/123456789/4506810.1016/j.ijbiomac.2019.10.087ElsevierEnzyme coimmobilizationEnzyme orderingCombilipasesCoimmobilization of different lipases: Simple layer by layer enzyme spatial orderinginfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2030-12This paper shows the step by step coimmobilization of up to five different enzymes following two different orders in the coimmobilization to alter the effect of substrate diffusion limitations. The enzymes were the lipases A and B from Candida antarctica, the lipases from Rhizomocur miehei and, Themomyces lanuginosus and the phospholipase Lecitase Ultra. The utilized strategy was a layer by layer immobilization, coating the immobilized enzymes with polyethylenimine followed by the crosslinking of the enzyme and PEI with glutaraldehyde to prevent enzyme release, and them adding a new lipase layer. The use of previously inactivated biocatalysts (using diethyl p-nitrophenylphosphate) permitted to visualize the immobilization of each enzyme layer, which was later confirmed by SDS-PAGE. This also confirmed the successful and complete covalent crosslinking of the glutaraldehyde treated enzyme layers. Activity of the combibiocatalysts was followed using diverse substrates. The protocol was successful and permitted to immobilize in an ordered way the 5 different enzymes in a down-up distributionengreponame:Repositório Institucional da UFRNinstname:Universidade Federal do Rio Grande do Norte (UFRN)instacron:UFRNinfo:eu-repo/semantics/openAccessCC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8914https://repositorio.ufrn.br/bitstream/123456789/45068/2/license_rdf4d2950bda3d176f570a9f8b328dfbbefMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81569https://repositorio.ufrn.br/bitstream/123456789/45068/3/license.txt6e6f57145bc87daf99079f06b081ff9fMD53123456789/450682024-03-19 01:02:00.312oai:https://repositorio.ufrn.br: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ório de PublicaçõesPUBhttp://repositorio.ufrn.br/oai/opendoar:2024-03-19T04:02Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)false
dc.title.pt_BR.fl_str_mv Coimmobilization of different lipases: Simple layer by layer enzyme spatial ordering
title Coimmobilization of different lipases: Simple layer by layer enzyme spatial ordering
spellingShingle Coimmobilization of different lipases: Simple layer by layer enzyme spatial ordering
Peña, Sara Arana
Enzyme coimmobilization
Enzyme ordering
Combilipases
title_short Coimmobilization of different lipases: Simple layer by layer enzyme spatial ordering
title_full Coimmobilization of different lipases: Simple layer by layer enzyme spatial ordering
title_fullStr Coimmobilization of different lipases: Simple layer by layer enzyme spatial ordering
title_full_unstemmed Coimmobilization of different lipases: Simple layer by layer enzyme spatial ordering
title_sort Coimmobilization of different lipases: Simple layer by layer enzyme spatial ordering
author Peña, Sara Arana
author_facet Peña, Sara Arana
Rios, Nathalia Saraiva
Sanchez, Carmen Mendez
Lokha, Yuliya
Carballares, Diego
Gonçalves, Luciana Rocha Barros
Lafuente, Roberto Fernandez
author_role author
author2 Rios, Nathalia Saraiva
Sanchez, Carmen Mendez
Lokha, Yuliya
Carballares, Diego
Gonçalves, Luciana Rocha Barros
Lafuente, Roberto Fernandez
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Peña, Sara Arana
Rios, Nathalia Saraiva
Sanchez, Carmen Mendez
Lokha, Yuliya
Carballares, Diego
Gonçalves, Luciana Rocha Barros
Lafuente, Roberto Fernandez
dc.subject.por.fl_str_mv Enzyme coimmobilization
Enzyme ordering
Combilipases
topic Enzyme coimmobilization
Enzyme ordering
Combilipases
description 2030-12
publishDate 2020
dc.date.issued.fl_str_mv 2020-02-15
dc.date.accessioned.fl_str_mv 2021-11-29T21:16:03Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv ARANA-PEÑA, Sara; RIOS, Nathalia S.; MENDEZ-SANCHEZ, Carmen; LOKHA, Yuliya; CARBALLARES, Diego; GONÇALVES, Luciana R.B.; FERNANDEZ-LAFUENTE, Roberto. Coimmobilization of different lipases: simple layer by layer enzyme spatial ordering. International Journal Of Biological Macromolecules, [S.L.], v. 145, p. 856-864, fev. 2020. Disponível em: https://www.sciencedirect.com/science/article/abs/pii/S0141813019371466?via%3Dihub#!. Acesso em: 29 nov. 2021. https://doi.org/10.1016/j.ijbiomac.2019.10.087.
dc.identifier.uri.fl_str_mv https://repositorio.ufrn.br/handle/123456789/45068
dc.identifier.issn.none.fl_str_mv 0141-8130
dc.identifier.doi.none.fl_str_mv 10.1016/j.ijbiomac.2019.10.087
identifier_str_mv ARANA-PEÑA, Sara; RIOS, Nathalia S.; MENDEZ-SANCHEZ, Carmen; LOKHA, Yuliya; CARBALLARES, Diego; GONÇALVES, Luciana R.B.; FERNANDEZ-LAFUENTE, Roberto. Coimmobilization of different lipases: simple layer by layer enzyme spatial ordering. International Journal Of Biological Macromolecules, [S.L.], v. 145, p. 856-864, fev. 2020. Disponível em: https://www.sciencedirect.com/science/article/abs/pii/S0141813019371466?via%3Dihub#!. Acesso em: 29 nov. 2021. https://doi.org/10.1016/j.ijbiomac.2019.10.087.
0141-8130
10.1016/j.ijbiomac.2019.10.087
url https://repositorio.ufrn.br/handle/123456789/45068
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFRN
instname:Universidade Federal do Rio Grande do Norte (UFRN)
instacron:UFRN
instname_str Universidade Federal do Rio Grande do Norte (UFRN)
instacron_str UFRN
institution UFRN
reponame_str Repositório Institucional da UFRN
collection Repositório Institucional da UFRN
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