Coimmobilization of different lipases: Simple layer by layer enzyme spatial ordering
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRN |
Texto Completo: | https://repositorio.ufrn.br/handle/123456789/45068 |
Resumo: | 2030-12 |
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Peña, Sara AranaRios, Nathalia SaraivaSanchez, Carmen MendezLokha, YuliyaCarballares, DiegoGonçalves, Luciana Rocha BarrosLafuente, Roberto Fernandez2021-11-29T21:16:03Z2020-02-15ARANA-PEÑA, Sara; RIOS, Nathalia S.; MENDEZ-SANCHEZ, Carmen; LOKHA, Yuliya; CARBALLARES, Diego; GONÇALVES, Luciana R.B.; FERNANDEZ-LAFUENTE, Roberto. Coimmobilization of different lipases: simple layer by layer enzyme spatial ordering. International Journal Of Biological Macromolecules, [S.L.], v. 145, p. 856-864, fev. 2020. Disponível em: https://www.sciencedirect.com/science/article/abs/pii/S0141813019371466?via%3Dihub#!. Acesso em: 29 nov. 2021. https://doi.org/10.1016/j.ijbiomac.2019.10.087.0141-8130https://repositorio.ufrn.br/handle/123456789/4506810.1016/j.ijbiomac.2019.10.087ElsevierEnzyme coimmobilizationEnzyme orderingCombilipasesCoimmobilization of different lipases: Simple layer by layer enzyme spatial orderinginfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2030-12This paper shows the step by step coimmobilization of up to five different enzymes following two different orders in the coimmobilization to alter the effect of substrate diffusion limitations. The enzymes were the lipases A and B from Candida antarctica, the lipases from Rhizomocur miehei and, Themomyces lanuginosus and the phospholipase Lecitase Ultra. The utilized strategy was a layer by layer immobilization, coating the immobilized enzymes with polyethylenimine followed by the crosslinking of the enzyme and PEI with glutaraldehyde to prevent enzyme release, and them adding a new lipase layer. The use of previously inactivated biocatalysts (using diethyl p-nitrophenylphosphate) permitted to visualize the immobilization of each enzyme layer, which was later confirmed by SDS-PAGE. This also confirmed the successful and complete covalent crosslinking of the glutaraldehyde treated enzyme layers. Activity of the combibiocatalysts was followed using diverse substrates. The protocol was successful and permitted to immobilize in an ordered way the 5 different enzymes in a down-up distributionengreponame:Repositório Institucional da UFRNinstname:Universidade Federal do Rio Grande do Norte (UFRN)instacron:UFRNinfo:eu-repo/semantics/openAccessCC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8914https://repositorio.ufrn.br/bitstream/123456789/45068/2/license_rdf4d2950bda3d176f570a9f8b328dfbbefMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81569https://repositorio.ufrn.br/bitstream/123456789/45068/3/license.txt6e6f57145bc87daf99079f06b081ff9fMD53123456789/450682024-03-19 01:02:00.312oai:https://repositorio.ufrn.br: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ório de PublicaçõesPUBhttp://repositorio.ufrn.br/oai/opendoar:2024-03-19T04:02Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)false |
dc.title.pt_BR.fl_str_mv |
Coimmobilization of different lipases: Simple layer by layer enzyme spatial ordering |
title |
Coimmobilization of different lipases: Simple layer by layer enzyme spatial ordering |
spellingShingle |
Coimmobilization of different lipases: Simple layer by layer enzyme spatial ordering Peña, Sara Arana Enzyme coimmobilization Enzyme ordering Combilipases |
title_short |
Coimmobilization of different lipases: Simple layer by layer enzyme spatial ordering |
title_full |
Coimmobilization of different lipases: Simple layer by layer enzyme spatial ordering |
title_fullStr |
Coimmobilization of different lipases: Simple layer by layer enzyme spatial ordering |
title_full_unstemmed |
Coimmobilization of different lipases: Simple layer by layer enzyme spatial ordering |
title_sort |
Coimmobilization of different lipases: Simple layer by layer enzyme spatial ordering |
author |
Peña, Sara Arana |
author_facet |
Peña, Sara Arana Rios, Nathalia Saraiva Sanchez, Carmen Mendez Lokha, Yuliya Carballares, Diego Gonçalves, Luciana Rocha Barros Lafuente, Roberto Fernandez |
author_role |
author |
author2 |
Rios, Nathalia Saraiva Sanchez, Carmen Mendez Lokha, Yuliya Carballares, Diego Gonçalves, Luciana Rocha Barros Lafuente, Roberto Fernandez |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Peña, Sara Arana Rios, Nathalia Saraiva Sanchez, Carmen Mendez Lokha, Yuliya Carballares, Diego Gonçalves, Luciana Rocha Barros Lafuente, Roberto Fernandez |
dc.subject.por.fl_str_mv |
Enzyme coimmobilization Enzyme ordering Combilipases |
topic |
Enzyme coimmobilization Enzyme ordering Combilipases |
description |
2030-12 |
publishDate |
2020 |
dc.date.issued.fl_str_mv |
2020-02-15 |
dc.date.accessioned.fl_str_mv |
2021-11-29T21:16:03Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
ARANA-PEÑA, Sara; RIOS, Nathalia S.; MENDEZ-SANCHEZ, Carmen; LOKHA, Yuliya; CARBALLARES, Diego; GONÇALVES, Luciana R.B.; FERNANDEZ-LAFUENTE, Roberto. Coimmobilization of different lipases: simple layer by layer enzyme spatial ordering. International Journal Of Biological Macromolecules, [S.L.], v. 145, p. 856-864, fev. 2020. Disponível em: https://www.sciencedirect.com/science/article/abs/pii/S0141813019371466?via%3Dihub#!. Acesso em: 29 nov. 2021. https://doi.org/10.1016/j.ijbiomac.2019.10.087. |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufrn.br/handle/123456789/45068 |
dc.identifier.issn.none.fl_str_mv |
0141-8130 |
dc.identifier.doi.none.fl_str_mv |
10.1016/j.ijbiomac.2019.10.087 |
identifier_str_mv |
ARANA-PEÑA, Sara; RIOS, Nathalia S.; MENDEZ-SANCHEZ, Carmen; LOKHA, Yuliya; CARBALLARES, Diego; GONÇALVES, Luciana R.B.; FERNANDEZ-LAFUENTE, Roberto. Coimmobilization of different lipases: simple layer by layer enzyme spatial ordering. International Journal Of Biological Macromolecules, [S.L.], v. 145, p. 856-864, fev. 2020. Disponível em: https://www.sciencedirect.com/science/article/abs/pii/S0141813019371466?via%3Dihub#!. Acesso em: 29 nov. 2021. https://doi.org/10.1016/j.ijbiomac.2019.10.087. 0141-8130 10.1016/j.ijbiomac.2019.10.087 |
url |
https://repositorio.ufrn.br/handle/123456789/45068 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFRN instname:Universidade Federal do Rio Grande do Norte (UFRN) instacron:UFRN |
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Universidade Federal do Rio Grande do Norte (UFRN) |
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UFRN |
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UFRN |
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Repositório Institucional da UFRN |
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Repositório Institucional da UFRN |
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