Utilization of agroindustrial residues for producing cellulases by Aspergillus fumigatus on Semi-Solid Fermentation
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRN |
Texto Completo: | https://repositorio.ufrn.br/handle/123456789/32627 |
Resumo: | Agroindustrial biomasses (sugarcane bagasse, green coconut husk and cashew apple bagasse) are used as substrate for producing cellulases by Aspergillus fumigatus on Semi-Solid Fermentation (SSF). The untreated lignocellulosics and fermented sugarcane bagasse are characterized using the National Renewable Energy Laboratory (NREL) protocols analysis as well as through Fourier Transform Infrared Spectroscopy (FTIR), X-ray diffraction (XRD) and scanning electron microscopy (SEM) assays. A two-variable central composite rotatable design (CCRD) coupled to response surface methodology (RSM) with three experiments at center point are used to investigate the influence of initial moisture and pH on carboxymethycellulose and filter paper, i.e., CMCase and FPase activities produced by the Aspergillus fumigatus using all of the three lignocellulosic biomasses. Results show that sugarcane bagasse has the highest cellulose content and the cashew apple bagasse the highest lignin content, approximately 46.0%. Additionally, pH and moisture (linear and quadratic parameters), as well as their combination, play a key role on both CMCase and FPase activities. The highest values for CMCase and FPase are 4.20 U/g and 0.64 U/g for sugarcane bagasse; 4.07 U/g and 0.28 U/g for coconut husk and 0.64 U/g and 0.32 U/g for cashew apple bagasse. The use of SSF for producing cellulases can be an option for reducing energy consumption due to reduction on the steps during biomass pretreatment |
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Santos, Everaldo Silvino dosOliveira, Sérgio DantasPadilha, Carlos Eduardo de AraújoAsevedo, Estefani AlvesPimentel, Vanessa CarvalhoAraújo, Fernanda Rodrigues deMacedo, Gorete Ribeiro de2021-06-10T13:54:54Z2021-06-10T13:54:54Z2018-02OLIVEIRA JUNIOR, S. D.; PADILHA, C. E.; ASEVEDO, E. A. ; PIMENTEL, V. C. ; ARAUJO, F. R. ; MACEDO, G. R. ; SANTOS, E. S. . Utilization of agroindustrial residues for producing cellulases by Aspergillus fumigatus on Semi-Solid Fermentation. Journal of Environmental Chemical Engineering, v. 6, p. 937-944, 2018. Disponível em: https://www.sciencedirect.com/science/article/abs/pii/S2213343717306772?via%3Dihub. Acesso em: 20 abr. 2021. https://doi.org/10.1016/j.jece.2017.12.0382213-3437https://repositorio.ufrn.br/handle/123456789/3262710.1016/j.jece.2017.12.038ElsevierAttribution 3.0 Brazilhttp://creativecommons.org/licenses/by/3.0/br/info:eu-repo/semantics/openAccessSemi-solid fermentationAspergillus fumigatusCoconut huskSugarcane bagasseCashew apple bagasseCellulasesUtilization of agroindustrial residues for producing cellulases by Aspergillus fumigatus on Semi-Solid Fermentationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleAgroindustrial biomasses (sugarcane bagasse, green coconut husk and cashew apple bagasse) are used as substrate for producing cellulases by Aspergillus fumigatus on Semi-Solid Fermentation (SSF). The untreated lignocellulosics and fermented sugarcane bagasse are characterized using the National Renewable Energy Laboratory (NREL) protocols analysis as well as through Fourier Transform Infrared Spectroscopy (FTIR), X-ray diffraction (XRD) and scanning electron microscopy (SEM) assays. A two-variable central composite rotatable design (CCRD) coupled to response surface methodology (RSM) with three experiments at center point are used to investigate the influence of initial moisture and pH on carboxymethycellulose and filter paper, i.e., CMCase and FPase activities produced by the Aspergillus fumigatus using all of the three lignocellulosic biomasses. Results show that sugarcane bagasse has the highest cellulose content and the cashew apple bagasse the highest lignin content, approximately 46.0%. Additionally, pH and moisture (linear and quadratic parameters), as well as their combination, play a key role on both CMCase and FPase activities. The highest values for CMCase and FPase are 4.20 U/g and 0.64 U/g for sugarcane bagasse; 4.07 U/g and 0.28 U/g for coconut husk and 0.64 U/g and 0.32 U/g for cashew apple bagasse. The use of SSF for producing cellulases can be an option for reducing energy consumption due to reduction on the steps during biomass pretreatmentengreponame:Repositório Institucional da UFRNinstname:Universidade Federal do Rio Grande do Norte (UFRN)instacron:UFRNORIGINALUtilizationOAgroindustrialResidues_Santos_2018.pdfUtilizationOAgroindustrialResidues_Santos_2018.pdfapplication/pdf3891073https://repositorio.ufrn.br/bitstream/123456789/32627/1/UtilizationOAgroindustrialResidues_Santos_2018.pdfeb2aef7680c726b22bc64065011f95ffMD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8914https://repositorio.ufrn.br/bitstream/123456789/32627/2/license_rdf4d2950bda3d176f570a9f8b328dfbbefMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81484https://repositorio.ufrn.br/bitstream/123456789/32627/3/license.txte9597aa2854d128fd968be5edc8a28d9MD53123456789/326272021-06-10 10:54:55.563oai:https://repositorio.ufrn.br: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Repositório de PublicaçõesPUBhttp://repositorio.ufrn.br/oai/opendoar:2021-06-10T13:54:55Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)false |
dc.title.pt_BR.fl_str_mv |
Utilization of agroindustrial residues for producing cellulases by Aspergillus fumigatus on Semi-Solid Fermentation |
title |
Utilization of agroindustrial residues for producing cellulases by Aspergillus fumigatus on Semi-Solid Fermentation |
spellingShingle |
Utilization of agroindustrial residues for producing cellulases by Aspergillus fumigatus on Semi-Solid Fermentation Santos, Everaldo Silvino dos Semi-solid fermentation Aspergillus fumigatus Coconut husk Sugarcane bagasse Cashew apple bagasse Cellulases |
title_short |
Utilization of agroindustrial residues for producing cellulases by Aspergillus fumigatus on Semi-Solid Fermentation |
title_full |
Utilization of agroindustrial residues for producing cellulases by Aspergillus fumigatus on Semi-Solid Fermentation |
title_fullStr |
Utilization of agroindustrial residues for producing cellulases by Aspergillus fumigatus on Semi-Solid Fermentation |
title_full_unstemmed |
Utilization of agroindustrial residues for producing cellulases by Aspergillus fumigatus on Semi-Solid Fermentation |
title_sort |
Utilization of agroindustrial residues for producing cellulases by Aspergillus fumigatus on Semi-Solid Fermentation |
author |
Santos, Everaldo Silvino dos |
author_facet |
Santos, Everaldo Silvino dos Oliveira, Sérgio Dantas Padilha, Carlos Eduardo de Araújo Asevedo, Estefani Alves Pimentel, Vanessa Carvalho Araújo, Fernanda Rodrigues de Macedo, Gorete Ribeiro de |
author_role |
author |
author2 |
Oliveira, Sérgio Dantas Padilha, Carlos Eduardo de Araújo Asevedo, Estefani Alves Pimentel, Vanessa Carvalho Araújo, Fernanda Rodrigues de Macedo, Gorete Ribeiro de |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Santos, Everaldo Silvino dos Oliveira, Sérgio Dantas Padilha, Carlos Eduardo de Araújo Asevedo, Estefani Alves Pimentel, Vanessa Carvalho Araújo, Fernanda Rodrigues de Macedo, Gorete Ribeiro de |
dc.subject.por.fl_str_mv |
Semi-solid fermentation Aspergillus fumigatus Coconut husk Sugarcane bagasse Cashew apple bagasse Cellulases |
topic |
Semi-solid fermentation Aspergillus fumigatus Coconut husk Sugarcane bagasse Cashew apple bagasse Cellulases |
description |
Agroindustrial biomasses (sugarcane bagasse, green coconut husk and cashew apple bagasse) are used as substrate for producing cellulases by Aspergillus fumigatus on Semi-Solid Fermentation (SSF). The untreated lignocellulosics and fermented sugarcane bagasse are characterized using the National Renewable Energy Laboratory (NREL) protocols analysis as well as through Fourier Transform Infrared Spectroscopy (FTIR), X-ray diffraction (XRD) and scanning electron microscopy (SEM) assays. A two-variable central composite rotatable design (CCRD) coupled to response surface methodology (RSM) with three experiments at center point are used to investigate the influence of initial moisture and pH on carboxymethycellulose and filter paper, i.e., CMCase and FPase activities produced by the Aspergillus fumigatus using all of the three lignocellulosic biomasses. Results show that sugarcane bagasse has the highest cellulose content and the cashew apple bagasse the highest lignin content, approximately 46.0%. Additionally, pH and moisture (linear and quadratic parameters), as well as their combination, play a key role on both CMCase and FPase activities. The highest values for CMCase and FPase are 4.20 U/g and 0.64 U/g for sugarcane bagasse; 4.07 U/g and 0.28 U/g for coconut husk and 0.64 U/g and 0.32 U/g for cashew apple bagasse. The use of SSF for producing cellulases can be an option for reducing energy consumption due to reduction on the steps during biomass pretreatment |
publishDate |
2018 |
dc.date.issued.fl_str_mv |
2018-02 |
dc.date.accessioned.fl_str_mv |
2021-06-10T13:54:54Z |
dc.date.available.fl_str_mv |
2021-06-10T13:54:54Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
OLIVEIRA JUNIOR, S. D.; PADILHA, C. E.; ASEVEDO, E. A. ; PIMENTEL, V. C. ; ARAUJO, F. R. ; MACEDO, G. R. ; SANTOS, E. S. . Utilization of agroindustrial residues for producing cellulases by Aspergillus fumigatus on Semi-Solid Fermentation. Journal of Environmental Chemical Engineering, v. 6, p. 937-944, 2018. Disponível em: https://www.sciencedirect.com/science/article/abs/pii/S2213343717306772?via%3Dihub. Acesso em: 20 abr. 2021. https://doi.org/10.1016/j.jece.2017.12.038 |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufrn.br/handle/123456789/32627 |
dc.identifier.issn.none.fl_str_mv |
2213-3437 |
dc.identifier.doi.none.fl_str_mv |
10.1016/j.jece.2017.12.038 |
identifier_str_mv |
OLIVEIRA JUNIOR, S. D.; PADILHA, C. E.; ASEVEDO, E. A. ; PIMENTEL, V. C. ; ARAUJO, F. R. ; MACEDO, G. R. ; SANTOS, E. S. . Utilization of agroindustrial residues for producing cellulases by Aspergillus fumigatus on Semi-Solid Fermentation. Journal of Environmental Chemical Engineering, v. 6, p. 937-944, 2018. Disponível em: https://www.sciencedirect.com/science/article/abs/pii/S2213343717306772?via%3Dihub. Acesso em: 20 abr. 2021. https://doi.org/10.1016/j.jece.2017.12.038 2213-3437 10.1016/j.jece.2017.12.038 |
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https://repositorio.ufrn.br/handle/123456789/32627 |
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Attribution 3.0 Brazil http://creativecommons.org/licenses/by/3.0/br/ |
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openAccess |
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Elsevier |
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Elsevier |
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