Utilization of agroindustrial residues for producing cellulases by Aspergillus fumigatus on Semi-Solid Fermentation

Detalhes bibliográficos
Autor(a) principal: Santos, Everaldo Silvino dos
Data de Publicação: 2018
Outros Autores: Oliveira, Sérgio Dantas, Padilha, Carlos Eduardo de Araújo, Asevedo, Estefani Alves, Pimentel, Vanessa Carvalho, Araújo, Fernanda Rodrigues de, Macedo, Gorete Ribeiro de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFRN
Texto Completo: https://repositorio.ufrn.br/handle/123456789/32627
Resumo: Agroindustrial biomasses (sugarcane bagasse, green coconut husk and cashew apple bagasse) are used as substrate for producing cellulases by Aspergillus fumigatus on Semi-Solid Fermentation (SSF). The untreated lignocellulosics and fermented sugarcane bagasse are characterized using the National Renewable Energy Laboratory (NREL) protocols analysis as well as through Fourier Transform Infrared Spectroscopy (FTIR), X-ray diffraction (XRD) and scanning electron microscopy (SEM) assays. A two-variable central composite rotatable design (CCRD) coupled to response surface methodology (RSM) with three experiments at center point are used to investigate the influence of initial moisture and pH on carboxymethycellulose and filter paper, i.e., CMCase and FPase activities produced by the Aspergillus fumigatus using all of the three lignocellulosic biomasses. Results show that sugarcane bagasse has the highest cellulose content and the cashew apple bagasse the highest lignin content, approximately 46.0%. Additionally, pH and moisture (linear and quadratic parameters), as well as their combination, play a key role on both CMCase and FPase activities. The highest values for CMCase and FPase are 4.20 U/g and 0.64 U/g for sugarcane bagasse; 4.07 U/g and 0.28 U/g for coconut husk and 0.64 U/g and 0.32 U/g for cashew apple bagasse. The use of SSF for producing cellulases can be an option for reducing energy consumption due to reduction on the steps during biomass pretreatment
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spelling Santos, Everaldo Silvino dosOliveira, Sérgio DantasPadilha, Carlos Eduardo de AraújoAsevedo, Estefani AlvesPimentel, Vanessa CarvalhoAraújo, Fernanda Rodrigues deMacedo, Gorete Ribeiro de2021-06-10T13:54:54Z2021-06-10T13:54:54Z2018-02OLIVEIRA JUNIOR, S. D.; PADILHA, C. E.; ASEVEDO, E. A. ; PIMENTEL, V. C. ; ARAUJO, F. R. ; MACEDO, G. R. ; SANTOS, E. S. . Utilization of agroindustrial residues for producing cellulases by Aspergillus fumigatus on Semi-Solid Fermentation. Journal of Environmental Chemical Engineering, v. 6, p. 937-944, 2018. Disponível em: https://www.sciencedirect.com/science/article/abs/pii/S2213343717306772?via%3Dihub. Acesso em: 20 abr. 2021. https://doi.org/10.1016/j.jece.2017.12.0382213-3437https://repositorio.ufrn.br/handle/123456789/3262710.1016/j.jece.2017.12.038ElsevierAttribution 3.0 Brazilhttp://creativecommons.org/licenses/by/3.0/br/info:eu-repo/semantics/openAccessSemi-solid fermentationAspergillus fumigatusCoconut huskSugarcane bagasseCashew apple bagasseCellulasesUtilization of agroindustrial residues for producing cellulases by Aspergillus fumigatus on Semi-Solid Fermentationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleAgroindustrial biomasses (sugarcane bagasse, green coconut husk and cashew apple bagasse) are used as substrate for producing cellulases by Aspergillus fumigatus on Semi-Solid Fermentation (SSF). The untreated lignocellulosics and fermented sugarcane bagasse are characterized using the National Renewable Energy Laboratory (NREL) protocols analysis as well as through Fourier Transform Infrared Spectroscopy (FTIR), X-ray diffraction (XRD) and scanning electron microscopy (SEM) assays. A two-variable central composite rotatable design (CCRD) coupled to response surface methodology (RSM) with three experiments at center point are used to investigate the influence of initial moisture and pH on carboxymethycellulose and filter paper, i.e., CMCase and FPase activities produced by the Aspergillus fumigatus using all of the three lignocellulosic biomasses. Results show that sugarcane bagasse has the highest cellulose content and the cashew apple bagasse the highest lignin content, approximately 46.0%. Additionally, pH and moisture (linear and quadratic parameters), as well as their combination, play a key role on both CMCase and FPase activities. The highest values for CMCase and FPase are 4.20 U/g and 0.64 U/g for sugarcane bagasse; 4.07 U/g and 0.28 U/g for coconut husk and 0.64 U/g and 0.32 U/g for cashew apple bagasse. The use of SSF for producing cellulases can be an option for reducing energy consumption due to reduction on the steps during biomass pretreatmentengreponame:Repositório Institucional da UFRNinstname:Universidade Federal do Rio Grande do Norte (UFRN)instacron:UFRNORIGINALUtilizationOAgroindustrialResidues_Santos_2018.pdfUtilizationOAgroindustrialResidues_Santos_2018.pdfapplication/pdf3891073https://repositorio.ufrn.br/bitstream/123456789/32627/1/UtilizationOAgroindustrialResidues_Santos_2018.pdfeb2aef7680c726b22bc64065011f95ffMD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8914https://repositorio.ufrn.br/bitstream/123456789/32627/2/license_rdf4d2950bda3d176f570a9f8b328dfbbefMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81484https://repositorio.ufrn.br/bitstream/123456789/32627/3/license.txte9597aa2854d128fd968be5edc8a28d9MD53123456789/326272021-06-10 10:54:55.563oai:https://repositorio.ufrn.br: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Repositório de PublicaçõesPUBhttp://repositorio.ufrn.br/oai/opendoar:2021-06-10T13:54:55Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)false
dc.title.pt_BR.fl_str_mv Utilization of agroindustrial residues for producing cellulases by Aspergillus fumigatus on Semi-Solid Fermentation
title Utilization of agroindustrial residues for producing cellulases by Aspergillus fumigatus on Semi-Solid Fermentation
spellingShingle Utilization of agroindustrial residues for producing cellulases by Aspergillus fumigatus on Semi-Solid Fermentation
Santos, Everaldo Silvino dos
Semi-solid fermentation
Aspergillus fumigatus
Coconut husk
Sugarcane bagasse
Cashew apple bagasse
Cellulases
title_short Utilization of agroindustrial residues for producing cellulases by Aspergillus fumigatus on Semi-Solid Fermentation
title_full Utilization of agroindustrial residues for producing cellulases by Aspergillus fumigatus on Semi-Solid Fermentation
title_fullStr Utilization of agroindustrial residues for producing cellulases by Aspergillus fumigatus on Semi-Solid Fermentation
title_full_unstemmed Utilization of agroindustrial residues for producing cellulases by Aspergillus fumigatus on Semi-Solid Fermentation
title_sort Utilization of agroindustrial residues for producing cellulases by Aspergillus fumigatus on Semi-Solid Fermentation
author Santos, Everaldo Silvino dos
author_facet Santos, Everaldo Silvino dos
Oliveira, Sérgio Dantas
Padilha, Carlos Eduardo de Araújo
Asevedo, Estefani Alves
Pimentel, Vanessa Carvalho
Araújo, Fernanda Rodrigues de
Macedo, Gorete Ribeiro de
author_role author
author2 Oliveira, Sérgio Dantas
Padilha, Carlos Eduardo de Araújo
Asevedo, Estefani Alves
Pimentel, Vanessa Carvalho
Araújo, Fernanda Rodrigues de
Macedo, Gorete Ribeiro de
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Santos, Everaldo Silvino dos
Oliveira, Sérgio Dantas
Padilha, Carlos Eduardo de Araújo
Asevedo, Estefani Alves
Pimentel, Vanessa Carvalho
Araújo, Fernanda Rodrigues de
Macedo, Gorete Ribeiro de
dc.subject.por.fl_str_mv Semi-solid fermentation
Aspergillus fumigatus
Coconut husk
Sugarcane bagasse
Cashew apple bagasse
Cellulases
topic Semi-solid fermentation
Aspergillus fumigatus
Coconut husk
Sugarcane bagasse
Cashew apple bagasse
Cellulases
description Agroindustrial biomasses (sugarcane bagasse, green coconut husk and cashew apple bagasse) are used as substrate for producing cellulases by Aspergillus fumigatus on Semi-Solid Fermentation (SSF). The untreated lignocellulosics and fermented sugarcane bagasse are characterized using the National Renewable Energy Laboratory (NREL) protocols analysis as well as through Fourier Transform Infrared Spectroscopy (FTIR), X-ray diffraction (XRD) and scanning electron microscopy (SEM) assays. A two-variable central composite rotatable design (CCRD) coupled to response surface methodology (RSM) with three experiments at center point are used to investigate the influence of initial moisture and pH on carboxymethycellulose and filter paper, i.e., CMCase and FPase activities produced by the Aspergillus fumigatus using all of the three lignocellulosic biomasses. Results show that sugarcane bagasse has the highest cellulose content and the cashew apple bagasse the highest lignin content, approximately 46.0%. Additionally, pH and moisture (linear and quadratic parameters), as well as their combination, play a key role on both CMCase and FPase activities. The highest values for CMCase and FPase are 4.20 U/g and 0.64 U/g for sugarcane bagasse; 4.07 U/g and 0.28 U/g for coconut husk and 0.64 U/g and 0.32 U/g for cashew apple bagasse. The use of SSF for producing cellulases can be an option for reducing energy consumption due to reduction on the steps during biomass pretreatment
publishDate 2018
dc.date.issued.fl_str_mv 2018-02
dc.date.accessioned.fl_str_mv 2021-06-10T13:54:54Z
dc.date.available.fl_str_mv 2021-06-10T13:54:54Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.citation.fl_str_mv OLIVEIRA JUNIOR, S. D.; PADILHA, C. E.; ASEVEDO, E. A. ; PIMENTEL, V. C. ; ARAUJO, F. R. ; MACEDO, G. R. ; SANTOS, E. S. . Utilization of agroindustrial residues for producing cellulases by Aspergillus fumigatus on Semi-Solid Fermentation. Journal of Environmental Chemical Engineering, v. 6, p. 937-944, 2018. Disponível em: https://www.sciencedirect.com/science/article/abs/pii/S2213343717306772?via%3Dihub. Acesso em: 20 abr. 2021. https://doi.org/10.1016/j.jece.2017.12.038
dc.identifier.uri.fl_str_mv https://repositorio.ufrn.br/handle/123456789/32627
dc.identifier.issn.none.fl_str_mv 2213-3437
dc.identifier.doi.none.fl_str_mv 10.1016/j.jece.2017.12.038
identifier_str_mv OLIVEIRA JUNIOR, S. D.; PADILHA, C. E.; ASEVEDO, E. A. ; PIMENTEL, V. C. ; ARAUJO, F. R. ; MACEDO, G. R. ; SANTOS, E. S. . Utilization of agroindustrial residues for producing cellulases by Aspergillus fumigatus on Semi-Solid Fermentation. Journal of Environmental Chemical Engineering, v. 6, p. 937-944, 2018. Disponível em: https://www.sciencedirect.com/science/article/abs/pii/S2213343717306772?via%3Dihub. Acesso em: 20 abr. 2021. https://doi.org/10.1016/j.jece.2017.12.038
2213-3437
10.1016/j.jece.2017.12.038
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http://creativecommons.org/licenses/by/3.0/br/
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