Immunohistochemical expression of vimentin, calponin and HHF-35 in salivary gland tumors
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRN |
Texto Completo: | https://repositorio.ufrn.br/jspui/handle/123456789/23888 http://dx.doi.org/10.1590/S0103-64402007000300003 |
Resumo: | Myoepithelial cells present a complex immunophenotype, with the expression of proteins varying according to the stage of normal or neoplastic differentiation of the cell. In order to evaluate the immunohistochemical markers expressed by these cells, a panel of antibodies composed of vimentin, calponin and HHF-35 was applied to 28 salivary gland tumors. The results demonstrated a higher percent sensitivity of vimentin and calponin compared to HHF-35. However, calponin and HHF-35 presented a focal labeling pattern in contrast with the diffuse distribution of vimentin. The cells predominantly stained by all tested antibodies included nonluminal cells in duct-like and tubular structures, such as those seen in pleomorphic adenomas and adenoid cystic carcinomas, as well as cells in the cords and nests of polymorphous low-grade adenocarcinomas and peripheral cells of sheets and nests of myoepitheliomas. In conclusion, the combination of calponin and vimentin is suggested for the identification of myoepithelial cells in salivary gland tumors. |
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Souza, Lélia Batista deCavalcante, Roberta BarrosoLopes, Fernanda FerreiraSoares, Andréa FerreiraFreitas, Roseana de Almeida2017-09-15T13:44:14Z2017-09-15T13:44:14Z2007SOUZA, Lélia Batista de et al . Immunohistochemical expression of vimentin, calponin and HHF-35 in salivary gland tumors. Brazilian Dental Journal, v. 10, n. 3, p. 38-42, 2007.https://repositorio.ufrn.br/jspui/handle/123456789/23888http://dx.doi.org/10.1590/S0103-64402007000300003engMyoepithelial cellSalivary gland tumorsVimentinCalponinHHF-35Immunohistochemical expression of vimentin, calponin and HHF-35 in salivary gland tumorsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleMyoepithelial cells present a complex immunophenotype, with the expression of proteins varying according to the stage of normal or neoplastic differentiation of the cell. In order to evaluate the immunohistochemical markers expressed by these cells, a panel of antibodies composed of vimentin, calponin and HHF-35 was applied to 28 salivary gland tumors. The results demonstrated a higher percent sensitivity of vimentin and calponin compared to HHF-35. However, calponin and HHF-35 presented a focal labeling pattern in contrast with the diffuse distribution of vimentin. The cells predominantly stained by all tested antibodies included nonluminal cells in duct-like and tubular structures, such as those seen in pleomorphic adenomas and adenoid cystic carcinomas, as well as cells in the cords and nests of polymorphous low-grade adenocarcinomas and peripheral cells of sheets and nests of myoepitheliomas. In conclusion, the combination of calponin and vimentin is suggested for the identification of myoepithelial cells in salivary gland tumors.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRNinstname:Universidade Federal do Rio Grande do Norte (UFRN)instacron:UFRNORIGINALImmunohistochemicalExpressionVimentin_Souza_2007.pdfImmunohistochemicalExpressionVimentin_Souza_2007.pdfhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402007000300003&lng=en&nrm=iso&tlng=enapplication/pdf426692https://repositorio.ufrn.br/bitstream/123456789/23888/1/ImmunohistochemicalExpressionVimentin_Souza_2007.pdf3639f996a61c8306aa8d3db91c2b6b85MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://repositorio.ufrn.br/bitstream/123456789/23888/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52TEXTImmunohistochemical Expression of Vimentin.pdf.txtImmunohistochemical Expression of Vimentin.pdf.txtExtracted texttext/plain22587https://repositorio.ufrn.br/bitstream/123456789/23888/5/Immunohistochemical%20Expression%20of%20Vimentin.pdf.txtfda285e4a0874825c7eabe73fa9f81bcMD55THUMBNAILImmunohistochemical Expression of Vimentin.pdf.jpgImmunohistochemical Expression of Vimentin.pdf.jpgIM Thumbnailimage/jpeg8032https://repositorio.ufrn.br/bitstream/123456789/23888/6/Immunohistochemical%20Expression%20of%20Vimentin.pdf.jpg4455647eef2f095508afc5cd3aae5678MD56123456789/238882021-12-09 12:53:34.962oai:https://repositorio.ufrn.br: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Repositório de PublicaçõesPUBhttp://repositorio.ufrn.br/oai/opendoar:2021-12-09T15:53:34Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)false |
dc.title.pt_BR.fl_str_mv |
Immunohistochemical expression of vimentin, calponin and HHF-35 in salivary gland tumors |
title |
Immunohistochemical expression of vimentin, calponin and HHF-35 in salivary gland tumors |
spellingShingle |
Immunohistochemical expression of vimentin, calponin and HHF-35 in salivary gland tumors Souza, Lélia Batista de Myoepithelial cell Salivary gland tumors Vimentin Calponin HHF-35 |
title_short |
Immunohistochemical expression of vimentin, calponin and HHF-35 in salivary gland tumors |
title_full |
Immunohistochemical expression of vimentin, calponin and HHF-35 in salivary gland tumors |
title_fullStr |
Immunohistochemical expression of vimentin, calponin and HHF-35 in salivary gland tumors |
title_full_unstemmed |
Immunohistochemical expression of vimentin, calponin and HHF-35 in salivary gland tumors |
title_sort |
Immunohistochemical expression of vimentin, calponin and HHF-35 in salivary gland tumors |
author |
Souza, Lélia Batista de |
author_facet |
Souza, Lélia Batista de Cavalcante, Roberta Barroso Lopes, Fernanda Ferreira Soares, Andréa Ferreira Freitas, Roseana de Almeida |
author_role |
author |
author2 |
Cavalcante, Roberta Barroso Lopes, Fernanda Ferreira Soares, Andréa Ferreira Freitas, Roseana de Almeida |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Souza, Lélia Batista de Cavalcante, Roberta Barroso Lopes, Fernanda Ferreira Soares, Andréa Ferreira Freitas, Roseana de Almeida |
dc.subject.por.fl_str_mv |
Myoepithelial cell Salivary gland tumors Vimentin Calponin HHF-35 |
topic |
Myoepithelial cell Salivary gland tumors Vimentin Calponin HHF-35 |
description |
Myoepithelial cells present a complex immunophenotype, with the expression of proteins varying according to the stage of normal or neoplastic differentiation of the cell. In order to evaluate the immunohistochemical markers expressed by these cells, a panel of antibodies composed of vimentin, calponin and HHF-35 was applied to 28 salivary gland tumors. The results demonstrated a higher percent sensitivity of vimentin and calponin compared to HHF-35. However, calponin and HHF-35 presented a focal labeling pattern in contrast with the diffuse distribution of vimentin. The cells predominantly stained by all tested antibodies included nonluminal cells in duct-like and tubular structures, such as those seen in pleomorphic adenomas and adenoid cystic carcinomas, as well as cells in the cords and nests of polymorphous low-grade adenocarcinomas and peripheral cells of sheets and nests of myoepitheliomas. In conclusion, the combination of calponin and vimentin is suggested for the identification of myoepithelial cells in salivary gland tumors. |
publishDate |
2007 |
dc.date.issued.fl_str_mv |
2007 |
dc.date.accessioned.fl_str_mv |
2017-09-15T13:44:14Z |
dc.date.available.fl_str_mv |
2017-09-15T13:44:14Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
SOUZA, Lélia Batista de et al . Immunohistochemical expression of vimentin, calponin and HHF-35 in salivary gland tumors. Brazilian Dental Journal, v. 10, n. 3, p. 38-42, 2007. |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufrn.br/jspui/handle/123456789/23888 |
dc.identifier.doi.none.fl_str_mv |
http://dx.doi.org/10.1590/S0103-64402007000300003 |
identifier_str_mv |
SOUZA, Lélia Batista de et al . Immunohistochemical expression of vimentin, calponin and HHF-35 in salivary gland tumors. Brazilian Dental Journal, v. 10, n. 3, p. 38-42, 2007. |
url |
https://repositorio.ufrn.br/jspui/handle/123456789/23888 http://dx.doi.org/10.1590/S0103-64402007000300003 |
dc.language.iso.fl_str_mv |
eng |
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eng |
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info:eu-repo/semantics/openAccess |
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openAccess |
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