Simultaneous saccharification and fermentation of cactus pear biomass—evaluation of using different pretreatments

Detalhes bibliográficos
Autor(a) principal: Santos, Everaldo Silvino dos
Data de Publicação: 2016
Outros Autores: Souza Filho, Pedro Ferreira de, Ribeiro, Vitor Troccoli, Macedo, Gorete Ribeiro de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFRN
Texto Completo: https://repositorio.ufrn.br/handle/123456789/32467
Resumo: Lignocellulosic biomasses of two species of cactus pear (Opuntia ficus indica and Nopalea cochenillifera) were tested for the production of ethanol. Their ability to grow in water-stressed regions makes them eligible for reducing the production cost of biofuels in drought regions. The biomass was pretreated using three different strategies: alkaline hydrogen peroxide, alkaline using NaOH, and acid using H2SO4 followed by alkaline delignification with NaOH. Analyses of the composition, crystallinity, and enzymatic hydrolysis were performed on the material before and after pretreatment. An experimental design was used to evaluate the influence of temperature and initial cellulose concentration on the process of simultaneous saccharification and fermentation (SSF) using the pretreated material, which showed the best performance in previous tests, and two strains of Saccharomyces cerevisiae (PE-2 and LNF CA-11). Biomass characterization showed a content of 31.55% cellulose, 17.12% hemicelluloses, and 10.25% lignins for N. cochenillifera and 34.86% cellulose, 19.97% hemicelluloses, and 15.72% lignins for O. ficus indica. The alkaline pretreatment provided the best enzymatic saccharification yields. The SSF experiments resulted in the greatest cellulose-to-ethanol yields using strain PE-2 with the pretreated N. cochenillifera (93.81%) at 40 ◦C and an initial cellulose concentration of 4%. N. cochenillifera showed better yields than O. ficus indica, and the PE-2 strain performed better than CA-11. These results suggest a good potential for cactus pear cladodes in the production of cellulosic ethanol
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spelling Santos, Everaldo Silvino dosSouza Filho, Pedro Ferreira deRibeiro, Vitor TroccoliMacedo, Gorete Ribeiro de2021-05-10T13:18:08Z2021-05-10T13:18:08Z2016-10-30SOUZA FILHO, P.F.; RIBEIRO, V.T.; SANTOS, E. S.; MACEDO, G. R.. Simultaneous saccharification and fermentation of cactus pear biomass-evaluation of using different pretreatments. Industrial Crops and Products (Print), v. 89, p. 425-433, 2016. Disponivel em: https://www.sciencedirect.com/science/article/abs/pii/S092666901630348X?via%3Dihub. Acesso em: 10 maio 2021. https://doi.org/10.1016/j.indcrop.2016.05.028.0926-6690https://repositorio.ufrn.br/handle/123456789/3246710.1016/j.indcrop.2016.05.028Industrial Crops and ProductsCactus pearEthanolPretreatmentSSFEnzymatic hydrolysisSimultaneous saccharification and fermentation of cactus pear biomass—evaluation of using different pretreatmentsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleLignocellulosic biomasses of two species of cactus pear (Opuntia ficus indica and Nopalea cochenillifera) were tested for the production of ethanol. Their ability to grow in water-stressed regions makes them eligible for reducing the production cost of biofuels in drought regions. The biomass was pretreated using three different strategies: alkaline hydrogen peroxide, alkaline using NaOH, and acid using H2SO4 followed by alkaline delignification with NaOH. Analyses of the composition, crystallinity, and enzymatic hydrolysis were performed on the material before and after pretreatment. An experimental design was used to evaluate the influence of temperature and initial cellulose concentration on the process of simultaneous saccharification and fermentation (SSF) using the pretreated material, which showed the best performance in previous tests, and two strains of Saccharomyces cerevisiae (PE-2 and LNF CA-11). Biomass characterization showed a content of 31.55% cellulose, 17.12% hemicelluloses, and 10.25% lignins for N. cochenillifera and 34.86% cellulose, 19.97% hemicelluloses, and 15.72% lignins for O. ficus indica. The alkaline pretreatment provided the best enzymatic saccharification yields. The SSF experiments resulted in the greatest cellulose-to-ethanol yields using strain PE-2 with the pretreated N. cochenillifera (93.81%) at 40 ◦C and an initial cellulose concentration of 4%. N. cochenillifera showed better yields than O. ficus indica, and the PE-2 strain performed better than CA-11. These results suggest a good potential for cactus pear cladodes in the production of cellulosic ethanolengreponame:Repositório Institucional da UFRNinstname:Universidade Federal do Rio Grande do Norte (UFRN)instacron:UFRNinfo:eu-repo/semantics/openAccessCC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8914https://repositorio.ufrn.br/bitstream/123456789/32467/2/license_rdf4d2950bda3d176f570a9f8b328dfbbefMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81484https://repositorio.ufrn.br/bitstream/123456789/32467/3/license.txte9597aa2854d128fd968be5edc8a28d9MD53123456789/324672023-02-07 16:20:55.19oai:https://repositorio.ufrn.br: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Repositório de PublicaçõesPUBhttp://repositorio.ufrn.br/oai/opendoar:2023-02-07T19:20:55Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)false
dc.title.pt_BR.fl_str_mv Simultaneous saccharification and fermentation of cactus pear biomass—evaluation of using different pretreatments
title Simultaneous saccharification and fermentation of cactus pear biomass—evaluation of using different pretreatments
spellingShingle Simultaneous saccharification and fermentation of cactus pear biomass—evaluation of using different pretreatments
Santos, Everaldo Silvino dos
Cactus pear
Ethanol
Pretreatment
SSF
Enzymatic hydrolysis
title_short Simultaneous saccharification and fermentation of cactus pear biomass—evaluation of using different pretreatments
title_full Simultaneous saccharification and fermentation of cactus pear biomass—evaluation of using different pretreatments
title_fullStr Simultaneous saccharification and fermentation of cactus pear biomass—evaluation of using different pretreatments
title_full_unstemmed Simultaneous saccharification and fermentation of cactus pear biomass—evaluation of using different pretreatments
title_sort Simultaneous saccharification and fermentation of cactus pear biomass—evaluation of using different pretreatments
author Santos, Everaldo Silvino dos
author_facet Santos, Everaldo Silvino dos
Souza Filho, Pedro Ferreira de
Ribeiro, Vitor Troccoli
Macedo, Gorete Ribeiro de
author_role author
author2 Souza Filho, Pedro Ferreira de
Ribeiro, Vitor Troccoli
Macedo, Gorete Ribeiro de
author2_role author
author
author
dc.contributor.author.fl_str_mv Santos, Everaldo Silvino dos
Souza Filho, Pedro Ferreira de
Ribeiro, Vitor Troccoli
Macedo, Gorete Ribeiro de
dc.subject.por.fl_str_mv Cactus pear
Ethanol
Pretreatment
SSF
Enzymatic hydrolysis
topic Cactus pear
Ethanol
Pretreatment
SSF
Enzymatic hydrolysis
description Lignocellulosic biomasses of two species of cactus pear (Opuntia ficus indica and Nopalea cochenillifera) were tested for the production of ethanol. Their ability to grow in water-stressed regions makes them eligible for reducing the production cost of biofuels in drought regions. The biomass was pretreated using three different strategies: alkaline hydrogen peroxide, alkaline using NaOH, and acid using H2SO4 followed by alkaline delignification with NaOH. Analyses of the composition, crystallinity, and enzymatic hydrolysis were performed on the material before and after pretreatment. An experimental design was used to evaluate the influence of temperature and initial cellulose concentration on the process of simultaneous saccharification and fermentation (SSF) using the pretreated material, which showed the best performance in previous tests, and two strains of Saccharomyces cerevisiae (PE-2 and LNF CA-11). Biomass characterization showed a content of 31.55% cellulose, 17.12% hemicelluloses, and 10.25% lignins for N. cochenillifera and 34.86% cellulose, 19.97% hemicelluloses, and 15.72% lignins for O. ficus indica. The alkaline pretreatment provided the best enzymatic saccharification yields. The SSF experiments resulted in the greatest cellulose-to-ethanol yields using strain PE-2 with the pretreated N. cochenillifera (93.81%) at 40 ◦C and an initial cellulose concentration of 4%. N. cochenillifera showed better yields than O. ficus indica, and the PE-2 strain performed better than CA-11. These results suggest a good potential for cactus pear cladodes in the production of cellulosic ethanol
publishDate 2016
dc.date.issued.fl_str_mv 2016-10-30
dc.date.accessioned.fl_str_mv 2021-05-10T13:18:08Z
dc.date.available.fl_str_mv 2021-05-10T13:18:08Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv SOUZA FILHO, P.F.; RIBEIRO, V.T.; SANTOS, E. S.; MACEDO, G. R.. Simultaneous saccharification and fermentation of cactus pear biomass-evaluation of using different pretreatments. Industrial Crops and Products (Print), v. 89, p. 425-433, 2016. Disponivel em: https://www.sciencedirect.com/science/article/abs/pii/S092666901630348X?via%3Dihub. Acesso em: 10 maio 2021. https://doi.org/10.1016/j.indcrop.2016.05.028.
dc.identifier.uri.fl_str_mv https://repositorio.ufrn.br/handle/123456789/32467
dc.identifier.issn.none.fl_str_mv 0926-6690
dc.identifier.doi.none.fl_str_mv 10.1016/j.indcrop.2016.05.028
identifier_str_mv SOUZA FILHO, P.F.; RIBEIRO, V.T.; SANTOS, E. S.; MACEDO, G. R.. Simultaneous saccharification and fermentation of cactus pear biomass-evaluation of using different pretreatments. Industrial Crops and Products (Print), v. 89, p. 425-433, 2016. Disponivel em: https://www.sciencedirect.com/science/article/abs/pii/S092666901630348X?via%3Dihub. Acesso em: 10 maio 2021. https://doi.org/10.1016/j.indcrop.2016.05.028.
0926-6690
10.1016/j.indcrop.2016.05.028
url https://repositorio.ufrn.br/handle/123456789/32467
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dc.publisher.none.fl_str_mv Industrial Crops and Products
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