Simultaneous saccharification and fermentation of cactus pear biomass—evaluation of using different pretreatments
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRN |
Texto Completo: | https://repositorio.ufrn.br/handle/123456789/32467 |
Resumo: | Lignocellulosic biomasses of two species of cactus pear (Opuntia ficus indica and Nopalea cochenillifera) were tested for the production of ethanol. Their ability to grow in water-stressed regions makes them eligible for reducing the production cost of biofuels in drought regions. The biomass was pretreated using three different strategies: alkaline hydrogen peroxide, alkaline using NaOH, and acid using H2SO4 followed by alkaline delignification with NaOH. Analyses of the composition, crystallinity, and enzymatic hydrolysis were performed on the material before and after pretreatment. An experimental design was used to evaluate the influence of temperature and initial cellulose concentration on the process of simultaneous saccharification and fermentation (SSF) using the pretreated material, which showed the best performance in previous tests, and two strains of Saccharomyces cerevisiae (PE-2 and LNF CA-11). Biomass characterization showed a content of 31.55% cellulose, 17.12% hemicelluloses, and 10.25% lignins for N. cochenillifera and 34.86% cellulose, 19.97% hemicelluloses, and 15.72% lignins for O. ficus indica. The alkaline pretreatment provided the best enzymatic saccharification yields. The SSF experiments resulted in the greatest cellulose-to-ethanol yields using strain PE-2 with the pretreated N. cochenillifera (93.81%) at 40 ◦C and an initial cellulose concentration of 4%. N. cochenillifera showed better yields than O. ficus indica, and the PE-2 strain performed better than CA-11. These results suggest a good potential for cactus pear cladodes in the production of cellulosic ethanol |
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Santos, Everaldo Silvino dosSouza Filho, Pedro Ferreira deRibeiro, Vitor TroccoliMacedo, Gorete Ribeiro de2021-05-10T13:18:08Z2021-05-10T13:18:08Z2016-10-30SOUZA FILHO, P.F.; RIBEIRO, V.T.; SANTOS, E. S.; MACEDO, G. R.. Simultaneous saccharification and fermentation of cactus pear biomass-evaluation of using different pretreatments. Industrial Crops and Products (Print), v. 89, p. 425-433, 2016. Disponivel em: https://www.sciencedirect.com/science/article/abs/pii/S092666901630348X?via%3Dihub. Acesso em: 10 maio 2021. https://doi.org/10.1016/j.indcrop.2016.05.028.0926-6690https://repositorio.ufrn.br/handle/123456789/3246710.1016/j.indcrop.2016.05.028Industrial Crops and ProductsCactus pearEthanolPretreatmentSSFEnzymatic hydrolysisSimultaneous saccharification and fermentation of cactus pear biomass—evaluation of using different pretreatmentsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleLignocellulosic biomasses of two species of cactus pear (Opuntia ficus indica and Nopalea cochenillifera) were tested for the production of ethanol. Their ability to grow in water-stressed regions makes them eligible for reducing the production cost of biofuels in drought regions. The biomass was pretreated using three different strategies: alkaline hydrogen peroxide, alkaline using NaOH, and acid using H2SO4 followed by alkaline delignification with NaOH. Analyses of the composition, crystallinity, and enzymatic hydrolysis were performed on the material before and after pretreatment. An experimental design was used to evaluate the influence of temperature and initial cellulose concentration on the process of simultaneous saccharification and fermentation (SSF) using the pretreated material, which showed the best performance in previous tests, and two strains of Saccharomyces cerevisiae (PE-2 and LNF CA-11). Biomass characterization showed a content of 31.55% cellulose, 17.12% hemicelluloses, and 10.25% lignins for N. cochenillifera and 34.86% cellulose, 19.97% hemicelluloses, and 15.72% lignins for O. ficus indica. The alkaline pretreatment provided the best enzymatic saccharification yields. The SSF experiments resulted in the greatest cellulose-to-ethanol yields using strain PE-2 with the pretreated N. cochenillifera (93.81%) at 40 ◦C and an initial cellulose concentration of 4%. N. cochenillifera showed better yields than O. ficus indica, and the PE-2 strain performed better than CA-11. These results suggest a good potential for cactus pear cladodes in the production of cellulosic ethanolengreponame:Repositório Institucional da UFRNinstname:Universidade Federal do Rio Grande do Norte (UFRN)instacron:UFRNinfo:eu-repo/semantics/openAccessCC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8914https://repositorio.ufrn.br/bitstream/123456789/32467/2/license_rdf4d2950bda3d176f570a9f8b328dfbbefMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81484https://repositorio.ufrn.br/bitstream/123456789/32467/3/license.txte9597aa2854d128fd968be5edc8a28d9MD53123456789/324672023-02-07 16:20:55.19oai:https://repositorio.ufrn.br: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Repositório de PublicaçõesPUBhttp://repositorio.ufrn.br/oai/opendoar:2023-02-07T19:20:55Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)false |
dc.title.pt_BR.fl_str_mv |
Simultaneous saccharification and fermentation of cactus pear biomass—evaluation of using different pretreatments |
title |
Simultaneous saccharification and fermentation of cactus pear biomass—evaluation of using different pretreatments |
spellingShingle |
Simultaneous saccharification and fermentation of cactus pear biomass—evaluation of using different pretreatments Santos, Everaldo Silvino dos Cactus pear Ethanol Pretreatment SSF Enzymatic hydrolysis |
title_short |
Simultaneous saccharification and fermentation of cactus pear biomass—evaluation of using different pretreatments |
title_full |
Simultaneous saccharification and fermentation of cactus pear biomass—evaluation of using different pretreatments |
title_fullStr |
Simultaneous saccharification and fermentation of cactus pear biomass—evaluation of using different pretreatments |
title_full_unstemmed |
Simultaneous saccharification and fermentation of cactus pear biomass—evaluation of using different pretreatments |
title_sort |
Simultaneous saccharification and fermentation of cactus pear biomass—evaluation of using different pretreatments |
author |
Santos, Everaldo Silvino dos |
author_facet |
Santos, Everaldo Silvino dos Souza Filho, Pedro Ferreira de Ribeiro, Vitor Troccoli Macedo, Gorete Ribeiro de |
author_role |
author |
author2 |
Souza Filho, Pedro Ferreira de Ribeiro, Vitor Troccoli Macedo, Gorete Ribeiro de |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Santos, Everaldo Silvino dos Souza Filho, Pedro Ferreira de Ribeiro, Vitor Troccoli Macedo, Gorete Ribeiro de |
dc.subject.por.fl_str_mv |
Cactus pear Ethanol Pretreatment SSF Enzymatic hydrolysis |
topic |
Cactus pear Ethanol Pretreatment SSF Enzymatic hydrolysis |
description |
Lignocellulosic biomasses of two species of cactus pear (Opuntia ficus indica and Nopalea cochenillifera) were tested for the production of ethanol. Their ability to grow in water-stressed regions makes them eligible for reducing the production cost of biofuels in drought regions. The biomass was pretreated using three different strategies: alkaline hydrogen peroxide, alkaline using NaOH, and acid using H2SO4 followed by alkaline delignification with NaOH. Analyses of the composition, crystallinity, and enzymatic hydrolysis were performed on the material before and after pretreatment. An experimental design was used to evaluate the influence of temperature and initial cellulose concentration on the process of simultaneous saccharification and fermentation (SSF) using the pretreated material, which showed the best performance in previous tests, and two strains of Saccharomyces cerevisiae (PE-2 and LNF CA-11). Biomass characterization showed a content of 31.55% cellulose, 17.12% hemicelluloses, and 10.25% lignins for N. cochenillifera and 34.86% cellulose, 19.97% hemicelluloses, and 15.72% lignins for O. ficus indica. The alkaline pretreatment provided the best enzymatic saccharification yields. The SSF experiments resulted in the greatest cellulose-to-ethanol yields using strain PE-2 with the pretreated N. cochenillifera (93.81%) at 40 ◦C and an initial cellulose concentration of 4%. N. cochenillifera showed better yields than O. ficus indica, and the PE-2 strain performed better than CA-11. These results suggest a good potential for cactus pear cladodes in the production of cellulosic ethanol |
publishDate |
2016 |
dc.date.issued.fl_str_mv |
2016-10-30 |
dc.date.accessioned.fl_str_mv |
2021-05-10T13:18:08Z |
dc.date.available.fl_str_mv |
2021-05-10T13:18:08Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
SOUZA FILHO, P.F.; RIBEIRO, V.T.; SANTOS, E. S.; MACEDO, G. R.. Simultaneous saccharification and fermentation of cactus pear biomass-evaluation of using different pretreatments. Industrial Crops and Products (Print), v. 89, p. 425-433, 2016. Disponivel em: https://www.sciencedirect.com/science/article/abs/pii/S092666901630348X?via%3Dihub. Acesso em: 10 maio 2021. https://doi.org/10.1016/j.indcrop.2016.05.028. |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufrn.br/handle/123456789/32467 |
dc.identifier.issn.none.fl_str_mv |
0926-6690 |
dc.identifier.doi.none.fl_str_mv |
10.1016/j.indcrop.2016.05.028 |
identifier_str_mv |
SOUZA FILHO, P.F.; RIBEIRO, V.T.; SANTOS, E. S.; MACEDO, G. R.. Simultaneous saccharification and fermentation of cactus pear biomass-evaluation of using different pretreatments. Industrial Crops and Products (Print), v. 89, p. 425-433, 2016. Disponivel em: https://www.sciencedirect.com/science/article/abs/pii/S092666901630348X?via%3Dihub. Acesso em: 10 maio 2021. https://doi.org/10.1016/j.indcrop.2016.05.028. 0926-6690 10.1016/j.indcrop.2016.05.028 |
url |
https://repositorio.ufrn.br/handle/123456789/32467 |
dc.language.iso.fl_str_mv |
eng |
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eng |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Industrial Crops and Products |
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Industrial Crops and Products |
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