GluN2B and GluN2A containing NMDAR are differentially involved in extinction memory destabilization and restabilization during reconsolidation
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRN |
Texto Completo: | https://repositorio.ufrn.br/handle/123456789/31478 |
Resumo: | Extinction memory destabilized by recall is restabilized through mTOR-dependent reconsolidation in the hippocampus, but the upstream pathways controlling these processes remain unknown. Hippocampal NMDARs drive local protein synthesis via mTOR signaling and may control active memory maintenance. We found that in adult male Wistar rats, intra dorsal-CA1 administration of the non-subunit selective NMDAR antagonist AP5 or of the GluN2A subunit-containing NMDAR antagonist TCN201 after step down inhibitory avoidance (SDIA) extinction memory recall impaired extinction memory retention and caused SDIA memory recovery. On the contrary, pre-recall administration of AP5 or of the GluN2B subunit-containing NMDAR antagonist RO25-6981 had no effect on extinction memory recall or retention per se but hindered the recovery of the avoidance response induced by post-recall intra-CA1 infusion of the mTOR inhibitor rapamycin. Our results indicate that GluN2B-containing NMDARs are necessary for extinction memory destabilization whereas GluN2A-containing NMDARs are involved in its restabilization, and suggest that pharmacological modulation of the relative activation state of these receptor subtypes around the moment of extinction memory recall may regulate the dominance of extinction memory over the original memory trace |
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Radiske, AndressaGonzalez, Maria CarolinaFerreira, Diana Aline Nôga MoraisRossato, Janine InezBevilaqua, Lia Rejane MüllerCammarota, Martín Pablo2021-02-11T16:01:16Z2021-02-11T16:01:16Z2021-01-08RADISKE, Andressa; GONZALEZ, Maria Carolina; NÔGA, Diana A.; ROSSATO, Janine I.; BEVILAQUA, Lia R. M.; CAMMAROTA, Martín. GluN2B and GluN2A-containing NMDAR are differentially involved in extinction memory destabilization and restabilization during reconsolidation. Scientific Reports, [S. l.], v. 11, n. 1, p. 178-186, 8 jan. 2021. doi:10.1038/s41598-020-80674-7. Disponível em: https://www.nature.com/articles/s41598-020-80674-7. Acesso em: 11 fev. 2021.https://repositorio.ufrn.br/handle/123456789/3147810.1038/s41598-020-80674-7Springer Science and Business Media LLCAttribution 3.0 Brazilhttp://creativecommons.org/licenses/by/3.0/br/info:eu-repo/semantics/openAccessGluN2BGluN2ANMDARExtinction memoryMemory consolidationMemoryGluN2B and GluN2A containing NMDAR are differentially involved in extinction memory destabilization and restabilization during reconsolidationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleExtinction memory destabilized by recall is restabilized through mTOR-dependent reconsolidation in the hippocampus, but the upstream pathways controlling these processes remain unknown. Hippocampal NMDARs drive local protein synthesis via mTOR signaling and may control active memory maintenance. We found that in adult male Wistar rats, intra dorsal-CA1 administration of the non-subunit selective NMDAR antagonist AP5 or of the GluN2A subunit-containing NMDAR antagonist TCN201 after step down inhibitory avoidance (SDIA) extinction memory recall impaired extinction memory retention and caused SDIA memory recovery. On the contrary, pre-recall administration of AP5 or of the GluN2B subunit-containing NMDAR antagonist RO25-6981 had no effect on extinction memory recall or retention per se but hindered the recovery of the avoidance response induced by post-recall intra-CA1 infusion of the mTOR inhibitor rapamycin. Our results indicate that GluN2B-containing NMDARs are necessary for extinction memory destabilization whereas GluN2A-containing NMDARs are involved in its restabilization, and suggest that pharmacological modulation of the relative activation state of these receptor subtypes around the moment of extinction memory recall may regulate the dominance of extinction memory over the original memory traceengreponame:Repositório Institucional da UFRNinstname:Universidade Federal do Rio Grande do Norte (UFRN)instacron:UFRNORIGINALGluN2BGluN2A-containingNMDAR_Cammarota_2021.pdfGluN2BGluN2A-containingNMDAR_Cammarota_2021.pdfGluN2BGluN2A-containingNMDAR_Cammarota_2021application/pdf2394919https://repositorio.ufrn.br/bitstream/123456789/31478/1/GluN2BGluN2A-containingNMDAR_Cammarota_2021.pdf0c0a365e49f7dd1850d09e20a1d31c33MD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8914https://repositorio.ufrn.br/bitstream/123456789/31478/2/license_rdf4d2950bda3d176f570a9f8b328dfbbefMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81484https://repositorio.ufrn.br/bitstream/123456789/31478/3/license.txte9597aa2854d128fd968be5edc8a28d9MD53TEXTGluN2BGluN2A-containingNMDAR_Cammarota_2021.pdf.txtGluN2BGluN2A-containingNMDAR_Cammarota_2021.pdf.txtExtracted texttext/plain38472https://repositorio.ufrn.br/bitstream/123456789/31478/4/GluN2BGluN2A-containingNMDAR_Cammarota_2021.pdf.txtf4ddf9e887b0fd4651d91f4bfb5df681MD54THUMBNAILGluN2BGluN2A-containingNMDAR_Cammarota_2021.pdf.jpgGluN2BGluN2A-containingNMDAR_Cammarota_2021.pdf.jpgGenerated Thumbnailimage/jpeg1865https://repositorio.ufrn.br/bitstream/123456789/31478/5/GluN2BGluN2A-containingNMDAR_Cammarota_2021.pdf.jpg4923a03d51dbd13c046fa106ca7b546eMD55123456789/314782021-07-08 23:39:00.975oai:https://repositorio.ufrn.br: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Repositório de PublicaçõesPUBhttp://repositorio.ufrn.br/oai/opendoar:2021-07-09T02:39Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)false |
dc.title.pt_BR.fl_str_mv |
GluN2B and GluN2A containing NMDAR are differentially involved in extinction memory destabilization and restabilization during reconsolidation |
title |
GluN2B and GluN2A containing NMDAR are differentially involved in extinction memory destabilization and restabilization during reconsolidation |
spellingShingle |
GluN2B and GluN2A containing NMDAR are differentially involved in extinction memory destabilization and restabilization during reconsolidation Radiske, Andressa GluN2B GluN2A NMDAR Extinction memory Memory consolidation Memory |
title_short |
GluN2B and GluN2A containing NMDAR are differentially involved in extinction memory destabilization and restabilization during reconsolidation |
title_full |
GluN2B and GluN2A containing NMDAR are differentially involved in extinction memory destabilization and restabilization during reconsolidation |
title_fullStr |
GluN2B and GluN2A containing NMDAR are differentially involved in extinction memory destabilization and restabilization during reconsolidation |
title_full_unstemmed |
GluN2B and GluN2A containing NMDAR are differentially involved in extinction memory destabilization and restabilization during reconsolidation |
title_sort |
GluN2B and GluN2A containing NMDAR are differentially involved in extinction memory destabilization and restabilization during reconsolidation |
author |
Radiske, Andressa |
author_facet |
Radiske, Andressa Gonzalez, Maria Carolina Ferreira, Diana Aline Nôga Morais Rossato, Janine Inez Bevilaqua, Lia Rejane Müller Cammarota, Martín Pablo |
author_role |
author |
author2 |
Gonzalez, Maria Carolina Ferreira, Diana Aline Nôga Morais Rossato, Janine Inez Bevilaqua, Lia Rejane Müller Cammarota, Martín Pablo |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Radiske, Andressa Gonzalez, Maria Carolina Ferreira, Diana Aline Nôga Morais Rossato, Janine Inez Bevilaqua, Lia Rejane Müller Cammarota, Martín Pablo |
dc.subject.por.fl_str_mv |
GluN2B GluN2A NMDAR Extinction memory Memory consolidation Memory |
topic |
GluN2B GluN2A NMDAR Extinction memory Memory consolidation Memory |
description |
Extinction memory destabilized by recall is restabilized through mTOR-dependent reconsolidation in the hippocampus, but the upstream pathways controlling these processes remain unknown. Hippocampal NMDARs drive local protein synthesis via mTOR signaling and may control active memory maintenance. We found that in adult male Wistar rats, intra dorsal-CA1 administration of the non-subunit selective NMDAR antagonist AP5 or of the GluN2A subunit-containing NMDAR antagonist TCN201 after step down inhibitory avoidance (SDIA) extinction memory recall impaired extinction memory retention and caused SDIA memory recovery. On the contrary, pre-recall administration of AP5 or of the GluN2B subunit-containing NMDAR antagonist RO25-6981 had no effect on extinction memory recall or retention per se but hindered the recovery of the avoidance response induced by post-recall intra-CA1 infusion of the mTOR inhibitor rapamycin. Our results indicate that GluN2B-containing NMDARs are necessary for extinction memory destabilization whereas GluN2A-containing NMDARs are involved in its restabilization, and suggest that pharmacological modulation of the relative activation state of these receptor subtypes around the moment of extinction memory recall may regulate the dominance of extinction memory over the original memory trace |
publishDate |
2021 |
dc.date.accessioned.fl_str_mv |
2021-02-11T16:01:16Z |
dc.date.available.fl_str_mv |
2021-02-11T16:01:16Z |
dc.date.issued.fl_str_mv |
2021-01-08 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
RADISKE, Andressa; GONZALEZ, Maria Carolina; NÔGA, Diana A.; ROSSATO, Janine I.; BEVILAQUA, Lia R. M.; CAMMAROTA, Martín. GluN2B and GluN2A-containing NMDAR are differentially involved in extinction memory destabilization and restabilization during reconsolidation. Scientific Reports, [S. l.], v. 11, n. 1, p. 178-186, 8 jan. 2021. doi:10.1038/s41598-020-80674-7. Disponível em: https://www.nature.com/articles/s41598-020-80674-7. Acesso em: 11 fev. 2021. |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufrn.br/handle/123456789/31478 |
dc.identifier.doi.none.fl_str_mv |
10.1038/s41598-020-80674-7 |
identifier_str_mv |
RADISKE, Andressa; GONZALEZ, Maria Carolina; NÔGA, Diana A.; ROSSATO, Janine I.; BEVILAQUA, Lia R. M.; CAMMAROTA, Martín. GluN2B and GluN2A-containing NMDAR are differentially involved in extinction memory destabilization and restabilization during reconsolidation. Scientific Reports, [S. l.], v. 11, n. 1, p. 178-186, 8 jan. 2021. doi:10.1038/s41598-020-80674-7. Disponível em: https://www.nature.com/articles/s41598-020-80674-7. Acesso em: 11 fev. 2021. 10.1038/s41598-020-80674-7 |
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https://repositorio.ufrn.br/handle/123456789/31478 |
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eng |
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eng |
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Attribution 3.0 Brazil http://creativecommons.org/licenses/by/3.0/br/ info:eu-repo/semantics/openAccess |
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Attribution 3.0 Brazil http://creativecommons.org/licenses/by/3.0/br/ |
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openAccess |
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Springer Science and Business Media LLC |
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Springer Science and Business Media LLC |
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