Efeito inibitório do extrato hidroetanólico das raízes da Harpalyce brasiliana no envenenamento local induzido pela peçonha da serpente Bothrops leucurus
Autor(a) principal: | |
---|---|
Data de Publicação: | 2022 |
Tipo de documento: | Trabalho de conclusão de curso |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRN |
Texto Completo: | https://repositorio.ufrn.br/handle/123456789/48638 |
Resumo: | In northeastern Brazil the Bothrops leucurus is the main causative agent of snakebites. The symptoms of bothropic envenomation are severe pain, edema, hemorrhage, necrosis, amputations and even death. Antivenom serum is not accessible in many regions and present same limitations, requiring alternative therapies. The hydroethanolic extract from the roots of Harpalyce brasiliana has been used for decades as an alternative therapy for snakebites. Therefore, the aim of this study was to evaluate the ability of the hydroethanolic extract of H. brasiliana to inhibit the local effects of B. leucurus snake venom. For this, the assays of inhibition of the enzymatic activity of the venom were carried out in vitro. The evaluation of the inhibition of edematogenic, hemorrhagic and myotoxic activity was performed using experimental models in vivo. The results showed in the enzyme inhibition assays that the extract was not able to decrease phospholipases activity, however it was efficient in hyaluronidase and proteolytic inhibition activities of the venom. In all the concentrations used (100, 200 and 400 mg/kg) the extract was able to significantly reduce the percentage of edema formed, slightly higher than the antivenom serum. Similarly, there was a reduction in myeloperoxidase and pro-inflammatory interleukins levels. Finally, the extract also significantly inhibited the parameters of hemorrhagic and myotoxic activities. These data indicate a potential antiophidic activity of H. brasiliana in poisoning by B. leucurus, especially to reduce local poison effects. |
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Cavalcanti, Felipe França0000-0003-0971-044Xhttp://lattes.cnpq.br/13054457886449200000-0001-5514-5192http://lattes.cnpq.br/4163879756935913Rêgo, Manoela Torres do0000-0002-8535-1533http://lattes.cnpq.br/0735782214033188Silva, Alessandra Daniele0000-0003-1650-3836http://lattes.cnpq.br/1245062709759151Silva, Juliana Félix da0000-0003-3131-9025http://lattes.cnpq.br/7927574480239375Araujo, Renata Mendonça2022-07-21T20:49:43Z2022-07-21T20:49:43Z2022-07-01CAVALCANTI, Felipe França. Efeito inibitório do extrato hidroetanólico das raízes da Harpalyce brasiliana no envenenamento local induzido pela peçonha da serpente Bothrops leucurus. 2022. 56f. Trabalho de Conclusão de Curso (Graduação em Farmácia), Departamento de Farmácia, Universidade Federal do Rio Grande do Norte, Natal, 2022.https://repositorio.ufrn.br/handle/123456789/48638In northeastern Brazil the Bothrops leucurus is the main causative agent of snakebites. The symptoms of bothropic envenomation are severe pain, edema, hemorrhage, necrosis, amputations and even death. Antivenom serum is not accessible in many regions and present same limitations, requiring alternative therapies. The hydroethanolic extract from the roots of Harpalyce brasiliana has been used for decades as an alternative therapy for snakebites. Therefore, the aim of this study was to evaluate the ability of the hydroethanolic extract of H. brasiliana to inhibit the local effects of B. leucurus snake venom. For this, the assays of inhibition of the enzymatic activity of the venom were carried out in vitro. The evaluation of the inhibition of edematogenic, hemorrhagic and myotoxic activity was performed using experimental models in vivo. The results showed in the enzyme inhibition assays that the extract was not able to decrease phospholipases activity, however it was efficient in hyaluronidase and proteolytic inhibition activities of the venom. In all the concentrations used (100, 200 and 400 mg/kg) the extract was able to significantly reduce the percentage of edema formed, slightly higher than the antivenom serum. Similarly, there was a reduction in myeloperoxidase and pro-inflammatory interleukins levels. Finally, the extract also significantly inhibited the parameters of hemorrhagic and myotoxic activities. These data indicate a potential antiophidic activity of H. brasiliana in poisoning by B. leucurus, especially to reduce local poison effects.In northeastern Brazil the Bothrops leucurus is the main causative agent of snakebites. The symptoms of bothropic envenomation are severe pain, edema, hemorrhage, necrosis, amputations and even death. Antivenom serum is not accessible in many regions and present same limitations, requiring alternative therapies. The hydroethanolic extract from the roots of Harpalyce brasiliana has been used for decades as an alternative therapy for snakebites. Therefore, the aim of this study was to evaluate the ability of the hydroethanolic extract of H. brasiliana to inhibit the local effects of B. leucurus snake venom. For this, the assays of inhibition of the enzymatic activity of the venom were carried out in vitro. The evaluation of the inhibition of edematogenic, hemorrhagic and myotoxic activity was performed using experimental models in vivo. The results showed in the enzyme inhibition assays that the extract was not able to decrease phospholipases activity, however it was efficient in hyaluronidase and proteolytic inhibition activities of the venom. In all the concentrations used (100, 200 and 400 mg/kg) the extract was able to significantly reduce the percentage of edema formed, slightly higher than the antivenom serum. Similarly, there was a reduction in myeloperoxidase and pro-inflammatory interleukins levels. Finally, the extract also significantly inhibited the parameters of hemorrhagic and myotoxic activities. These data indicate a potential antiophidic activity of H. brasiliana in poisoning by B. leucurus, especially to reduce local poison effects.CNPqUniversidade Federal do Rio Grande do NorteFarmáciaUFRNBrasilDepartamento de FarmáciaAttribution-NonCommercial-NoDerivs 3.0 Brazilhttp://creativecommons.org/licenses/by-nc-nd/3.0/br/info:eu-repo/semantics/openAccessBothrops leucurusHarpalyce brasilianaSnakebiteFolk medicineEfeito inibitório do extrato hidroetanólico das raízes da Harpalyce brasiliana no envenenamento local induzido pela peçonha da serpente Bothrops leucurusInhibitory effect of hydroethanol extract from Harpalyce brasiliana roots on local envenomation induced by Bothrops leucurus snake venominfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/bachelorThesisengreponame:Repositório Institucional da UFRNinstname:Universidade Federal do Rio Grande do Norte (UFRN)instacron:UFRNCC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8811https://repositorio.ufrn.br/bitstream/123456789/48638/2/license_rdfe39d27027a6cc9cb039ad269a5db8e34MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81484https://repositorio.ufrn.br/bitstream/123456789/48638/3/license.txte9597aa2854d128fd968be5edc8a28d9MD53ORIGINALCavalcanti(artigo).pdfCavalcanti(artigo).pdfapplication/pdf1701111https://repositorio.ufrn.br/bitstream/123456789/48638/1/Cavalcanti%28artigo%29.pdf27ea72de1ec67ee1a6cfc6571350b547MD51123456789/486382022-07-21 17:49:43.582oai:https://repositorio.ufrn.br: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Repositório de PublicaçõesPUBhttp://repositorio.ufrn.br/oai/opendoar:2022-07-21T20:49:43Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)false |
dc.title.pt_BR.fl_str_mv |
Efeito inibitório do extrato hidroetanólico das raízes da Harpalyce brasiliana no envenenamento local induzido pela peçonha da serpente Bothrops leucurus |
dc.title.alternative.pt_BR.fl_str_mv |
Inhibitory effect of hydroethanol extract from Harpalyce brasiliana roots on local envenomation induced by Bothrops leucurus snake venom |
title |
Efeito inibitório do extrato hidroetanólico das raízes da Harpalyce brasiliana no envenenamento local induzido pela peçonha da serpente Bothrops leucurus |
spellingShingle |
Efeito inibitório do extrato hidroetanólico das raízes da Harpalyce brasiliana no envenenamento local induzido pela peçonha da serpente Bothrops leucurus Cavalcanti, Felipe França Bothrops leucurus Harpalyce brasiliana Snakebite Folk medicine |
title_short |
Efeito inibitório do extrato hidroetanólico das raízes da Harpalyce brasiliana no envenenamento local induzido pela peçonha da serpente Bothrops leucurus |
title_full |
Efeito inibitório do extrato hidroetanólico das raízes da Harpalyce brasiliana no envenenamento local induzido pela peçonha da serpente Bothrops leucurus |
title_fullStr |
Efeito inibitório do extrato hidroetanólico das raízes da Harpalyce brasiliana no envenenamento local induzido pela peçonha da serpente Bothrops leucurus |
title_full_unstemmed |
Efeito inibitório do extrato hidroetanólico das raízes da Harpalyce brasiliana no envenenamento local induzido pela peçonha da serpente Bothrops leucurus |
title_sort |
Efeito inibitório do extrato hidroetanólico das raízes da Harpalyce brasiliana no envenenamento local induzido pela peçonha da serpente Bothrops leucurus |
author |
Cavalcanti, Felipe França |
author_facet |
Cavalcanti, Felipe França |
author_role |
author |
dc.contributor.authorID.pt_BR.fl_str_mv |
0000-0003-0971-044X |
dc.contributor.authorLattes.pt_BR.fl_str_mv |
http://lattes.cnpq.br/1305445788644920 |
dc.contributor.advisorID.pt_BR.fl_str_mv |
0000-0001-5514-5192 |
dc.contributor.advisorLattes.pt_BR.fl_str_mv |
http://lattes.cnpq.br/4163879756935913 |
dc.contributor.referees1.none.fl_str_mv |
Silva, Alessandra Daniele |
dc.contributor.referees1ID.pt_BR.fl_str_mv |
0000-0003-1650-3836 |
dc.contributor.referees1Lattes.pt_BR.fl_str_mv |
http://lattes.cnpq.br/1245062709759151 |
dc.contributor.referees2.none.fl_str_mv |
Silva, Juliana Félix da |
dc.contributor.referees2ID.pt_BR.fl_str_mv |
0000-0003-3131-9025 |
dc.contributor.referees2Lattes.pt_BR.fl_str_mv |
http://lattes.cnpq.br/7927574480239375 |
dc.contributor.author.fl_str_mv |
Cavalcanti, Felipe França |
dc.contributor.advisor-co1.fl_str_mv |
Rêgo, Manoela Torres do |
dc.contributor.advisor-co1ID.fl_str_mv |
0000-0002-8535-1533 |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://lattes.cnpq.br/0735782214033188 |
dc.contributor.advisor1.fl_str_mv |
Araujo, Renata Mendonça |
contributor_str_mv |
Rêgo, Manoela Torres do Araujo, Renata Mendonça |
dc.subject.por.fl_str_mv |
Bothrops leucurus Harpalyce brasiliana Snakebite Folk medicine |
topic |
Bothrops leucurus Harpalyce brasiliana Snakebite Folk medicine |
description |
In northeastern Brazil the Bothrops leucurus is the main causative agent of snakebites. The symptoms of bothropic envenomation are severe pain, edema, hemorrhage, necrosis, amputations and even death. Antivenom serum is not accessible in many regions and present same limitations, requiring alternative therapies. The hydroethanolic extract from the roots of Harpalyce brasiliana has been used for decades as an alternative therapy for snakebites. Therefore, the aim of this study was to evaluate the ability of the hydroethanolic extract of H. brasiliana to inhibit the local effects of B. leucurus snake venom. For this, the assays of inhibition of the enzymatic activity of the venom were carried out in vitro. The evaluation of the inhibition of edematogenic, hemorrhagic and myotoxic activity was performed using experimental models in vivo. The results showed in the enzyme inhibition assays that the extract was not able to decrease phospholipases activity, however it was efficient in hyaluronidase and proteolytic inhibition activities of the venom. In all the concentrations used (100, 200 and 400 mg/kg) the extract was able to significantly reduce the percentage of edema formed, slightly higher than the antivenom serum. Similarly, there was a reduction in myeloperoxidase and pro-inflammatory interleukins levels. Finally, the extract also significantly inhibited the parameters of hemorrhagic and myotoxic activities. These data indicate a potential antiophidic activity of H. brasiliana in poisoning by B. leucurus, especially to reduce local poison effects. |
publishDate |
2022 |
dc.date.accessioned.fl_str_mv |
2022-07-21T20:49:43Z |
dc.date.available.fl_str_mv |
2022-07-21T20:49:43Z |
dc.date.issued.fl_str_mv |
2022-07-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/bachelorThesis |
format |
bachelorThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
CAVALCANTI, Felipe França. Efeito inibitório do extrato hidroetanólico das raízes da Harpalyce brasiliana no envenenamento local induzido pela peçonha da serpente Bothrops leucurus. 2022. 56f. Trabalho de Conclusão de Curso (Graduação em Farmácia), Departamento de Farmácia, Universidade Federal do Rio Grande do Norte, Natal, 2022. |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufrn.br/handle/123456789/48638 |
identifier_str_mv |
CAVALCANTI, Felipe França. Efeito inibitório do extrato hidroetanólico das raízes da Harpalyce brasiliana no envenenamento local induzido pela peçonha da serpente Bothrops leucurus. 2022. 56f. Trabalho de Conclusão de Curso (Graduação em Farmácia), Departamento de Farmácia, Universidade Federal do Rio Grande do Norte, Natal, 2022. |
url |
https://repositorio.ufrn.br/handle/123456789/48638 |
dc.language.iso.fl_str_mv |
eng |
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eng |
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Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ info:eu-repo/semantics/openAccess |
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Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ |
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openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal do Rio Grande do Norte |
dc.publisher.program.fl_str_mv |
Farmácia |
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UFRN |
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Brasil |
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Departamento de Farmácia |
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Universidade Federal do Rio Grande do Norte |
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