Libidibia ferrea presents antiproliferative, apoptotic and antioxidant effects in a colorectal cancer cell line
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFRN |
Texto Completo: | https://repositorio.ufrn.br/handle/123456789/30221 |
Resumo: | Colorectal cancer is noted for being one of the most frequent of tumors, with expressive morbidity and mortality rates. In new drug discovery, plants stand out as a source capable of yielding safe and highefficiency products. Well known in Brazilian popular medicine, Libidibia ferrea (Mart. Ex Tul.) L.P. Queiroz var. ferrea (better known as “ironwood” or “jucá”), has been used to treat a wide spectrum of conditions and to prevent cancer. Using methodologies that involved flow cytometry, spectrophotometry and RT-qPCR assays, crude extracts of the fruits of L. ferrea (20T, 40T, 60T and 80T) were evaluated at 24 h and/or 48 h for: their ability to inhibit cell proliferation; induce apoptosis through Bcl-2, caspase-3 and Apaf-1; their antioxidant activity and effects on important targets related to cell proliferation (EGFR and AKT) in the HT-29 human colorectal cancer lineage. The results revealed high antiproliferative potential as compared to the controls, induction of apoptosis through the intrinsic pathway, and probable tumor inhibition activity under the mediation of important targets in tumorigenesis. In addition, L. ferrea revealed antioxidant, lipid peroxidation and chemoprotective effects in healthy cells. Thus, L. ferrea derivatives have important anticancer effects, and may be considered promising candidate for colorectal cancer therapy. |
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Araújo, Aurigena Antunes deGuerra, Andreza Conceição Véras de AguiarSoares, Luiz Alberto LiraFerreira, Magda Rhayanny AssunçãoRocha, Hugo Alexandre de OliveiraMedeirose, Juliana Silva deCavalcante, Rômulo dos SantosAraújo Júnior, Raimundo Fernandes de2020-09-28T18:09:32Z2020-09-28T18:09:32Z2017-08ARAÚJO, Aurigena Antunes de et al.Libidibia ferrea presents antiproliferative, apoptotic and antioxidant effects in a colorectal cancer cell line. Biomedicine & Pharmacotherapy, v. 92, p. 696-706, 2017. Disponível em: <https://www.sciencedirect.com/science/article/pii/S0753332217310946?via%3Dihub>. Acesso em: 21 mar. 2018. https://doi.org/10.1016/j.biopha.2017.05.1230753-3322https://repositorio.ufrn.br/handle/123456789/3022110.1016/j.biopha.2017.05.123ElsevierLibidibia ferreaColorectal cancerApoptosisAntioxidantLibidibia ferrea presents antiproliferative, apoptotic and antioxidant effects in a colorectal cancer cell lineinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleColorectal cancer is noted for being one of the most frequent of tumors, with expressive morbidity and mortality rates. In new drug discovery, plants stand out as a source capable of yielding safe and highefficiency products. Well known in Brazilian popular medicine, Libidibia ferrea (Mart. Ex Tul.) L.P. Queiroz var. ferrea (better known as “ironwood” or “jucá”), has been used to treat a wide spectrum of conditions and to prevent cancer. Using methodologies that involved flow cytometry, spectrophotometry and RT-qPCR assays, crude extracts of the fruits of L. ferrea (20T, 40T, 60T and 80T) were evaluated at 24 h and/or 48 h for: their ability to inhibit cell proliferation; induce apoptosis through Bcl-2, caspase-3 and Apaf-1; their antioxidant activity and effects on important targets related to cell proliferation (EGFR and AKT) in the HT-29 human colorectal cancer lineage. The results revealed high antiproliferative potential as compared to the controls, induction of apoptosis through the intrinsic pathway, and probable tumor inhibition activity under the mediation of important targets in tumorigenesis. In addition, L. ferrea revealed antioxidant, lipid peroxidation and chemoprotective effects in healthy cells. Thus, L. ferrea derivatives have important anticancer effects, and may be considered promising candidate for colorectal cancer therapy.info:eu-repo/semantics/openAccessporreponame:Repositório Institucional da UFRNinstname:Universidade Federal do Rio Grande do Norte (UFRN)instacron:UFRNTEXTLibidibiaFerreaPresentsAntiproliferative_2017.pdf.txtLibidibiaFerreaPresentsAntiproliferative_2017.pdf.txtExtracted texttext/plain47646https://repositorio.ufrn.br/bitstream/123456789/30221/3/LibidibiaFerreaPresentsAntiproliferative_2017.pdf.txt99d6bf0f28b6d2033525dfe9a68e3c6aMD53THUMBNAILLibidibiaFerreaPresentsAntiproliferative_2017.pdf.jpgLibidibiaFerreaPresentsAntiproliferative_2017.pdf.jpgGenerated Thumbnailimage/jpeg1733https://repositorio.ufrn.br/bitstream/123456789/30221/4/LibidibiaFerreaPresentsAntiproliferative_2017.pdf.jpge1c449bc4f0b409eb8e2b95a2d781317MD54LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://repositorio.ufrn.br/bitstream/123456789/30221/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52123456789/302212023-01-26 15:19:52.672oai:https://repositorio.ufrn.br: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Repositório de PublicaçõesPUBhttp://repositorio.ufrn.br/oai/opendoar:2023-01-26T18:19:52Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)false |
dc.title.pt_BR.fl_str_mv |
Libidibia ferrea presents antiproliferative, apoptotic and antioxidant effects in a colorectal cancer cell line |
title |
Libidibia ferrea presents antiproliferative, apoptotic and antioxidant effects in a colorectal cancer cell line |
spellingShingle |
Libidibia ferrea presents antiproliferative, apoptotic and antioxidant effects in a colorectal cancer cell line Araújo, Aurigena Antunes de Libidibia ferrea Colorectal cancer Apoptosis Antioxidant |
title_short |
Libidibia ferrea presents antiproliferative, apoptotic and antioxidant effects in a colorectal cancer cell line |
title_full |
Libidibia ferrea presents antiproliferative, apoptotic and antioxidant effects in a colorectal cancer cell line |
title_fullStr |
Libidibia ferrea presents antiproliferative, apoptotic and antioxidant effects in a colorectal cancer cell line |
title_full_unstemmed |
Libidibia ferrea presents antiproliferative, apoptotic and antioxidant effects in a colorectal cancer cell line |
title_sort |
Libidibia ferrea presents antiproliferative, apoptotic and antioxidant effects in a colorectal cancer cell line |
author |
Araújo, Aurigena Antunes de |
author_facet |
Araújo, Aurigena Antunes de Guerra, Andreza Conceição Véras de Aguiar Soares, Luiz Alberto Lira Ferreira, Magda Rhayanny Assunção Rocha, Hugo Alexandre de Oliveira Medeirose, Juliana Silva de Cavalcante, Rômulo dos Santos Araújo Júnior, Raimundo Fernandes de |
author_role |
author |
author2 |
Guerra, Andreza Conceição Véras de Aguiar Soares, Luiz Alberto Lira Ferreira, Magda Rhayanny Assunção Rocha, Hugo Alexandre de Oliveira Medeirose, Juliana Silva de Cavalcante, Rômulo dos Santos Araújo Júnior, Raimundo Fernandes de |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Araújo, Aurigena Antunes de Guerra, Andreza Conceição Véras de Aguiar Soares, Luiz Alberto Lira Ferreira, Magda Rhayanny Assunção Rocha, Hugo Alexandre de Oliveira Medeirose, Juliana Silva de Cavalcante, Rômulo dos Santos Araújo Júnior, Raimundo Fernandes de |
dc.subject.por.fl_str_mv |
Libidibia ferrea Colorectal cancer Apoptosis Antioxidant |
topic |
Libidibia ferrea Colorectal cancer Apoptosis Antioxidant |
description |
Colorectal cancer is noted for being one of the most frequent of tumors, with expressive morbidity and mortality rates. In new drug discovery, plants stand out as a source capable of yielding safe and highefficiency products. Well known in Brazilian popular medicine, Libidibia ferrea (Mart. Ex Tul.) L.P. Queiroz var. ferrea (better known as “ironwood” or “jucá”), has been used to treat a wide spectrum of conditions and to prevent cancer. Using methodologies that involved flow cytometry, spectrophotometry and RT-qPCR assays, crude extracts of the fruits of L. ferrea (20T, 40T, 60T and 80T) were evaluated at 24 h and/or 48 h for: their ability to inhibit cell proliferation; induce apoptosis through Bcl-2, caspase-3 and Apaf-1; their antioxidant activity and effects on important targets related to cell proliferation (EGFR and AKT) in the HT-29 human colorectal cancer lineage. The results revealed high antiproliferative potential as compared to the controls, induction of apoptosis through the intrinsic pathway, and probable tumor inhibition activity under the mediation of important targets in tumorigenesis. In addition, L. ferrea revealed antioxidant, lipid peroxidation and chemoprotective effects in healthy cells. Thus, L. ferrea derivatives have important anticancer effects, and may be considered promising candidate for colorectal cancer therapy. |
publishDate |
2017 |
dc.date.issued.fl_str_mv |
2017-08 |
dc.date.accessioned.fl_str_mv |
2020-09-28T18:09:32Z |
dc.date.available.fl_str_mv |
2020-09-28T18:09:32Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
ARAÚJO, Aurigena Antunes de et al.Libidibia ferrea presents antiproliferative, apoptotic and antioxidant effects in a colorectal cancer cell line. Biomedicine & Pharmacotherapy, v. 92, p. 696-706, 2017. Disponível em: <https://www.sciencedirect.com/science/article/pii/S0753332217310946?via%3Dihub>. Acesso em: 21 mar. 2018. https://doi.org/10.1016/j.biopha.2017.05.123 |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufrn.br/handle/123456789/30221 |
dc.identifier.issn.none.fl_str_mv |
0753-3322 |
dc.identifier.doi.none.fl_str_mv |
10.1016/j.biopha.2017.05.123 |
identifier_str_mv |
ARAÚJO, Aurigena Antunes de et al.Libidibia ferrea presents antiproliferative, apoptotic and antioxidant effects in a colorectal cancer cell line. Biomedicine & Pharmacotherapy, v. 92, p. 696-706, 2017. Disponível em: <https://www.sciencedirect.com/science/article/pii/S0753332217310946?via%3Dihub>. Acesso em: 21 mar. 2018. https://doi.org/10.1016/j.biopha.2017.05.123 0753-3322 10.1016/j.biopha.2017.05.123 |
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https://repositorio.ufrn.br/handle/123456789/30221 |
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por |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Elsevier |
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Elsevier |
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