Effect of trypsin inhibitor from Crotalaria pallida seeds on Callosobruchus maculatus (cowpea weevil) and Ceratitis capitata (fruit fly)

Detalhes bibliográficos
Autor(a) principal: Gomes, Carlos E. M.
Data de Publicação: 2005
Outros Autores: Barbosa, Aulus E. A. D., Macedo, Leonardo L. P., Pitanga, Joelma C. M., Moura, Fabiano T., Oliveira, Adeliana S., Moura, Raniere M., Queiroz, Alexandre F. S., Macedo, Francisco P., Andrade, Lúcia B. S., Vidal, Márcia S., Sales, Mauricio P.
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da UFRN
Texto Completo: https://repositorio.ufrn.br/jspui/handle/1/3089
Resumo: GOMES, Carlos E. M. et al. Effect of trypsin inhibitor from Crotalaria pallida seeds on Callosobruchus maculatus (cowpea weevil) and Ceratitis capitata (fruit fly). Plant Physiology and Biochemistry (Paris), v. 43, n. 12, p. 1095-1102, 2005.ISSN 0981-9428. DOI:10.1016/j.plaphy.2005.11.004.
id UFRN_ed4192dc9eb8bc33eb852e55daacdd09
oai_identifier_str oai:https://repositorio.ufrn.br:1/3089
network_acronym_str UFRN
network_name_str Repositório Institucional da UFRN
repository_id_str
spelling Gomes, Carlos E. M.Barbosa, Aulus E. A. D.Macedo, Leonardo L. P.Pitanga, Joelma C. M.Moura, Fabiano T.Oliveira, Adeliana S.Moura, Raniere M.Queiroz, Alexandre F. S.Macedo, Francisco P.Andrade, Lúcia B. S.Vidal, Márcia S.Sales, Mauricio P.2010-09-30T12:26:54Z2010-09-30T12:26:54Z2005-12-07GOMES, C. E. M.; BARBOSA, A. E. A. D.; MACEDO, L. L. P.; PITANGA, J. C. M.; MOURA, F. T.; OLIVEIRA, A. S.; MOURA, R. M.; QUEIROZ, A. F. S.; MACEDO, F. P.; ANDRADE, L. B. S.; VIDAL, M. S.; SALES, M. P. (2005)0981-9428https://repositorio.ufrn.br/jspui/handle/1/3089GOMES, Carlos E. M. et al. Effect of trypsin inhibitor from Crotalaria pallida seeds on Callosobruchus maculatus (cowpea weevil) and Ceratitis capitata (fruit fly). Plant Physiology and Biochemistry (Paris), v. 43, n. 12, p. 1095-1102, 2005.ISSN 0981-9428. DOI:10.1016/j.plaphy.2005.11.004.A proteinaceous trypsin inhibitor was purified from Crotalaria pallida seeds by ammonium sulfate precipitation, affinity chromatography on immobilized trypsin–Sepharose and TCA precipitation. The trypsin inhibitor, named CpaTI, had Mr of 32.5 kDa as determined by SDS-PAGE and was composed of two subunits with 27.7 and 5.6 kDa linked by disulfide bridges. CpaTI was stable at 50 °C and lost 40% of activity at 100 °C. CpaTI was also stable from pH 2 to 12 at 37 °C. CpaTI weakly inhibited chymotrypsin and lastase and its inhibition of papain, a cysteine proteinase, were indicative of its bi-functionality. CpaTI inhibited, in different degrees, digestive enzymes from Spodoptera frugiperda, Alabama argillacea, Plodia interpunctella, Anthonomus grandis and Zabrotes subfasciatus guts. In vitro and in vivo susceptibility of Calloso-bruchus maculatus and Ceratitis capitata to CpaTI was evaluated. C. maculatus and C. capitata enzymes were strongly susceptible, 74.4± 15.8% and 100.0 ± 7.3%, respectively, to CpaTI. When CpaTI was added to artificial diets and offered to both insect larvae, the results showed that C. maculatus was more susceptible to CpaTI with an LD50 of 3.0 and ED50 of 2.17%. C. capitata larvae were more resistant to CpaTI, in disagreement with the in vitro effects. The larvae were more affected at lower concentrations, causing 27% mortality and 44.4% mass decrease. The action was constant at 2–4% (w/w) with 15% mortality and 38% mass decrease.porPlant Physiology and BiochemistryTrypsin inhibitorCrotalaria pallidaCeratitis capitataCallosobruchus maculatusResistanceEffect of trypsin inhibitor from Crotalaria pallida seeds on Callosobruchus maculatus (cowpea weevil) and Ceratitis capitata (fruit fly)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRNinstname:Universidade Federal do Rio Grande do Norte (UFRN)instacron:UFRNORIGINALEffectTrypsinInhibitor_Gomes_2005.pdfEffectTrypsinInhibitor_Gomes_2005.pdfapplication/pdf320839https://repositorio.ufrn.br/bitstream/1/3089/1/EffectTrypsinInhibitor_Gomes_2005.pdfc48d366e80649fb92fef953d11321f24MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://repositorio.ufrn.br/bitstream/1/3089/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52TEXT2005Art_Effect_FranciscoPM.txt2005Art_Effect_FranciscoPM.txtExtracted texttext/plain43708https://repositorio.ufrn.br/bitstream/1/3089/7/2005Art_Effect_FranciscoPM.txtd4d8fde74c23ac8822d5ecd9f9b4be15MD57THUMBNAIL2005Art_Effect_FranciscoPM.jpg2005Art_Effect_FranciscoPM.jpgIM Thumbnailimage/jpeg11164https://repositorio.ufrn.br/bitstream/1/3089/8/2005Art_Effect_FranciscoPM.jpgbff14cb998029ee29cc3a9ec20e9a043MD581/30892021-11-08 16:57:09.356oai:https://repositorio.ufrn.br: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Repositório de PublicaçõesPUBhttp://repositorio.ufrn.br/oai/opendoar:2021-11-08T19:57:09Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)false
dc.title.pt_BR.fl_str_mv Effect of trypsin inhibitor from Crotalaria pallida seeds on Callosobruchus maculatus (cowpea weevil) and Ceratitis capitata (fruit fly)
title Effect of trypsin inhibitor from Crotalaria pallida seeds on Callosobruchus maculatus (cowpea weevil) and Ceratitis capitata (fruit fly)
spellingShingle Effect of trypsin inhibitor from Crotalaria pallida seeds on Callosobruchus maculatus (cowpea weevil) and Ceratitis capitata (fruit fly)
Gomes, Carlos E. M.
Trypsin inhibitor
Crotalaria pallida
Ceratitis capitata
Callosobruchus maculatus
Resistance
title_short Effect of trypsin inhibitor from Crotalaria pallida seeds on Callosobruchus maculatus (cowpea weevil) and Ceratitis capitata (fruit fly)
title_full Effect of trypsin inhibitor from Crotalaria pallida seeds on Callosobruchus maculatus (cowpea weevil) and Ceratitis capitata (fruit fly)
title_fullStr Effect of trypsin inhibitor from Crotalaria pallida seeds on Callosobruchus maculatus (cowpea weevil) and Ceratitis capitata (fruit fly)
title_full_unstemmed Effect of trypsin inhibitor from Crotalaria pallida seeds on Callosobruchus maculatus (cowpea weevil) and Ceratitis capitata (fruit fly)
title_sort Effect of trypsin inhibitor from Crotalaria pallida seeds on Callosobruchus maculatus (cowpea weevil) and Ceratitis capitata (fruit fly)
author Gomes, Carlos E. M.
author_facet Gomes, Carlos E. M.
Barbosa, Aulus E. A. D.
Macedo, Leonardo L. P.
Pitanga, Joelma C. M.
Moura, Fabiano T.
Oliveira, Adeliana S.
Moura, Raniere M.
Queiroz, Alexandre F. S.
Macedo, Francisco P.
Andrade, Lúcia B. S.
Vidal, Márcia S.
Sales, Mauricio P.
author_role author
author2 Barbosa, Aulus E. A. D.
Macedo, Leonardo L. P.
Pitanga, Joelma C. M.
Moura, Fabiano T.
Oliveira, Adeliana S.
Moura, Raniere M.
Queiroz, Alexandre F. S.
Macedo, Francisco P.
Andrade, Lúcia B. S.
Vidal, Márcia S.
Sales, Mauricio P.
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Gomes, Carlos E. M.
Barbosa, Aulus E. A. D.
Macedo, Leonardo L. P.
Pitanga, Joelma C. M.
Moura, Fabiano T.
Oliveira, Adeliana S.
Moura, Raniere M.
Queiroz, Alexandre F. S.
Macedo, Francisco P.
Andrade, Lúcia B. S.
Vidal, Márcia S.
Sales, Mauricio P.
dc.subject.por.fl_str_mv Trypsin inhibitor
Crotalaria pallida
Ceratitis capitata
Callosobruchus maculatus
Resistance
topic Trypsin inhibitor
Crotalaria pallida
Ceratitis capitata
Callosobruchus maculatus
Resistance
description GOMES, Carlos E. M. et al. Effect of trypsin inhibitor from Crotalaria pallida seeds on Callosobruchus maculatus (cowpea weevil) and Ceratitis capitata (fruit fly). Plant Physiology and Biochemistry (Paris), v. 43, n. 12, p. 1095-1102, 2005.ISSN 0981-9428. DOI:10.1016/j.plaphy.2005.11.004.
publishDate 2005
dc.date.issued.fl_str_mv 2005-12-07
dc.date.accessioned.fl_str_mv 2010-09-30T12:26:54Z
dc.date.available.fl_str_mv 2010-09-30T12:26:54Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv GOMES, C. E. M.; BARBOSA, A. E. A. D.; MACEDO, L. L. P.; PITANGA, J. C. M.; MOURA, F. T.; OLIVEIRA, A. S.; MOURA, R. M.; QUEIROZ, A. F. S.; MACEDO, F. P.; ANDRADE, L. B. S.; VIDAL, M. S.; SALES, M. P. (2005)
dc.identifier.uri.fl_str_mv https://repositorio.ufrn.br/jspui/handle/1/3089
dc.identifier.issn.none.fl_str_mv 0981-9428
identifier_str_mv GOMES, C. E. M.; BARBOSA, A. E. A. D.; MACEDO, L. L. P.; PITANGA, J. C. M.; MOURA, F. T.; OLIVEIRA, A. S.; MOURA, R. M.; QUEIROZ, A. F. S.; MACEDO, F. P.; ANDRADE, L. B. S.; VIDAL, M. S.; SALES, M. P. (2005)
0981-9428
url https://repositorio.ufrn.br/jspui/handle/1/3089
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Plant Physiology and Biochemistry
publisher.none.fl_str_mv Plant Physiology and Biochemistry
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFRN
instname:Universidade Federal do Rio Grande do Norte (UFRN)
instacron:UFRN
instname_str Universidade Federal do Rio Grande do Norte (UFRN)
instacron_str UFRN
institution UFRN
reponame_str Repositório Institucional da UFRN
collection Repositório Institucional da UFRN
bitstream.url.fl_str_mv https://repositorio.ufrn.br/bitstream/1/3089/1/EffectTrypsinInhibitor_Gomes_2005.pdf
https://repositorio.ufrn.br/bitstream/1/3089/2/license.txt
https://repositorio.ufrn.br/bitstream/1/3089/7/2005Art_Effect_FranciscoPM.txt
https://repositorio.ufrn.br/bitstream/1/3089/8/2005Art_Effect_FranciscoPM.jpg
bitstream.checksum.fl_str_mv c48d366e80649fb92fef953d11321f24
8a4605be74aa9ea9d79846c1fba20a33
d4d8fde74c23ac8822d5ecd9f9b4be15
bff14cb998029ee29cc3a9ec20e9a043
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)
repository.mail.fl_str_mv
_version_ 1802117675924586496

Registros relacionados