Effect of trypsin inhibitor from Crotalaria pallida seeds on Callosobruchus maculatus (cowpea weevil) and Ceratitis capitata (fruit fly)

Detalhes bibliográficos
Autor(a) principal: Gomes, Carlos E. M.
Data de Publicação: 2005
Outros Autores: Barbosa, Aulus E. A. D., Macedo, Leonardo L. P., Pitanga, Joelma C. M., Moura, Fabiano T., Oliveira, Adeliana S., Moura, Raniere M., Queiroz, Alexandre F. S., Macedo, Francisco P., Andrade, Lúcia B. S., Vidal, Márcia S., Sales, Mauricio P.
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da UFRN
Texto Completo: https://repositorio.ufrn.br/jspui/handle/1/3089
Resumo: GOMES, Carlos E. M. et al. Effect of trypsin inhibitor from Crotalaria pallida seeds on Callosobruchus maculatus (cowpea weevil) and Ceratitis capitata (fruit fly). Plant Physiology and Biochemistry (Paris), v. 43, n. 12, p. 1095-1102, 2005.ISSN 0981-9428. DOI:10.1016/j.plaphy.2005.11.004.
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spelling Gomes, Carlos E. M.Barbosa, Aulus E. A. D.Macedo, Leonardo L. P.Pitanga, Joelma C. M.Moura, Fabiano T.Oliveira, Adeliana S.Moura, Raniere M.Queiroz, Alexandre F. S.Macedo, Francisco P.Andrade, Lúcia B. S.Vidal, Márcia S.Sales, Mauricio P.2010-09-30T12:26:54Z2010-09-30T12:26:54Z2005-12-07GOMES, C. E. M.; BARBOSA, A. E. A. D.; MACEDO, L. L. P.; PITANGA, J. C. M.; MOURA, F. T.; OLIVEIRA, A. S.; MOURA, R. M.; QUEIROZ, A. F. S.; MACEDO, F. P.; ANDRADE, L. B. S.; VIDAL, M. S.; SALES, M. P. (2005)0981-9428https://repositorio.ufrn.br/jspui/handle/1/3089GOMES, Carlos E. M. et al. Effect of trypsin inhibitor from Crotalaria pallida seeds on Callosobruchus maculatus (cowpea weevil) and Ceratitis capitata (fruit fly). Plant Physiology and Biochemistry (Paris), v. 43, n. 12, p. 1095-1102, 2005.ISSN 0981-9428. DOI:10.1016/j.plaphy.2005.11.004.A proteinaceous trypsin inhibitor was purified from Crotalaria pallida seeds by ammonium sulfate precipitation, affinity chromatography on immobilized trypsin–Sepharose and TCA precipitation. The trypsin inhibitor, named CpaTI, had Mr of 32.5 kDa as determined by SDS-PAGE and was composed of two subunits with 27.7 and 5.6 kDa linked by disulfide bridges. CpaTI was stable at 50 °C and lost 40% of activity at 100 °C. CpaTI was also stable from pH 2 to 12 at 37 °C. CpaTI weakly inhibited chymotrypsin and lastase and its inhibition of papain, a cysteine proteinase, were indicative of its bi-functionality. CpaTI inhibited, in different degrees, digestive enzymes from Spodoptera frugiperda, Alabama argillacea, Plodia interpunctella, Anthonomus grandis and Zabrotes subfasciatus guts. In vitro and in vivo susceptibility of Calloso-bruchus maculatus and Ceratitis capitata to CpaTI was evaluated. C. maculatus and C. capitata enzymes were strongly susceptible, 74.4± 15.8% and 100.0 ± 7.3%, respectively, to CpaTI. When CpaTI was added to artificial diets and offered to both insect larvae, the results showed that C. maculatus was more susceptible to CpaTI with an LD50 of 3.0 and ED50 of 2.17%. C. capitata larvae were more resistant to CpaTI, in disagreement with the in vitro effects. The larvae were more affected at lower concentrations, causing 27% mortality and 44.4% mass decrease. The action was constant at 2–4% (w/w) with 15% mortality and 38% mass decrease.porPlant Physiology and BiochemistryTrypsin inhibitorCrotalaria pallidaCeratitis capitataCallosobruchus maculatusResistanceEffect of trypsin inhibitor from Crotalaria pallida seeds on Callosobruchus maculatus (cowpea weevil) and Ceratitis capitata (fruit fly)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRNinstname:Universidade Federal do Rio Grande do Norte (UFRN)instacron:UFRNORIGINALEffectTrypsinInhibitor_Gomes_2005.pdfEffectTrypsinInhibitor_Gomes_2005.pdfapplication/pdf320839https://repositorio.ufrn.br/bitstream/1/3089/1/EffectTrypsinInhibitor_Gomes_2005.pdfc48d366e80649fb92fef953d11321f24MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://repositorio.ufrn.br/bitstream/1/3089/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52TEXT2005Art_Effect_FranciscoPM.txt2005Art_Effect_FranciscoPM.txtExtracted texttext/plain43708https://repositorio.ufrn.br/bitstream/1/3089/7/2005Art_Effect_FranciscoPM.txtd4d8fde74c23ac8822d5ecd9f9b4be15MD57THUMBNAIL2005Art_Effect_FranciscoPM.jpg2005Art_Effect_FranciscoPM.jpgIM Thumbnailimage/jpeg11164https://repositorio.ufrn.br/bitstream/1/3089/8/2005Art_Effect_FranciscoPM.jpgbff14cb998029ee29cc3a9ec20e9a043MD581/30892021-11-08 16:57:09.356oai:https://repositorio.ufrn.br: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Repositório de PublicaçõesPUBhttp://repositorio.ufrn.br/oai/opendoar:2021-11-08T19:57:09Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)false
dc.title.pt_BR.fl_str_mv Effect of trypsin inhibitor from Crotalaria pallida seeds on Callosobruchus maculatus (cowpea weevil) and Ceratitis capitata (fruit fly)
title Effect of trypsin inhibitor from Crotalaria pallida seeds on Callosobruchus maculatus (cowpea weevil) and Ceratitis capitata (fruit fly)
spellingShingle Effect of trypsin inhibitor from Crotalaria pallida seeds on Callosobruchus maculatus (cowpea weevil) and Ceratitis capitata (fruit fly)
Gomes, Carlos E. M.
Trypsin inhibitor
Crotalaria pallida
Ceratitis capitata
Callosobruchus maculatus
Resistance
title_short Effect of trypsin inhibitor from Crotalaria pallida seeds on Callosobruchus maculatus (cowpea weevil) and Ceratitis capitata (fruit fly)
title_full Effect of trypsin inhibitor from Crotalaria pallida seeds on Callosobruchus maculatus (cowpea weevil) and Ceratitis capitata (fruit fly)
title_fullStr Effect of trypsin inhibitor from Crotalaria pallida seeds on Callosobruchus maculatus (cowpea weevil) and Ceratitis capitata (fruit fly)
title_full_unstemmed Effect of trypsin inhibitor from Crotalaria pallida seeds on Callosobruchus maculatus (cowpea weevil) and Ceratitis capitata (fruit fly)
title_sort Effect of trypsin inhibitor from Crotalaria pallida seeds on Callosobruchus maculatus (cowpea weevil) and Ceratitis capitata (fruit fly)
author Gomes, Carlos E. M.
author_facet Gomes, Carlos E. M.
Barbosa, Aulus E. A. D.
Macedo, Leonardo L. P.
Pitanga, Joelma C. M.
Moura, Fabiano T.
Oliveira, Adeliana S.
Moura, Raniere M.
Queiroz, Alexandre F. S.
Macedo, Francisco P.
Andrade, Lúcia B. S.
Vidal, Márcia S.
Sales, Mauricio P.
author_role author
author2 Barbosa, Aulus E. A. D.
Macedo, Leonardo L. P.
Pitanga, Joelma C. M.
Moura, Fabiano T.
Oliveira, Adeliana S.
Moura, Raniere M.
Queiroz, Alexandre F. S.
Macedo, Francisco P.
Andrade, Lúcia B. S.
Vidal, Márcia S.
Sales, Mauricio P.
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Gomes, Carlos E. M.
Barbosa, Aulus E. A. D.
Macedo, Leonardo L. P.
Pitanga, Joelma C. M.
Moura, Fabiano T.
Oliveira, Adeliana S.
Moura, Raniere M.
Queiroz, Alexandre F. S.
Macedo, Francisco P.
Andrade, Lúcia B. S.
Vidal, Márcia S.
Sales, Mauricio P.
dc.subject.por.fl_str_mv Trypsin inhibitor
Crotalaria pallida
Ceratitis capitata
Callosobruchus maculatus
Resistance
topic Trypsin inhibitor
Crotalaria pallida
Ceratitis capitata
Callosobruchus maculatus
Resistance
description GOMES, Carlos E. M. et al. Effect of trypsin inhibitor from Crotalaria pallida seeds on Callosobruchus maculatus (cowpea weevil) and Ceratitis capitata (fruit fly). Plant Physiology and Biochemistry (Paris), v. 43, n. 12, p. 1095-1102, 2005.ISSN 0981-9428. DOI:10.1016/j.plaphy.2005.11.004.
publishDate 2005
dc.date.issued.fl_str_mv 2005-12-07
dc.date.accessioned.fl_str_mv 2010-09-30T12:26:54Z
dc.date.available.fl_str_mv 2010-09-30T12:26:54Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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dc.identifier.citation.fl_str_mv GOMES, C. E. M.; BARBOSA, A. E. A. D.; MACEDO, L. L. P.; PITANGA, J. C. M.; MOURA, F. T.; OLIVEIRA, A. S.; MOURA, R. M.; QUEIROZ, A. F. S.; MACEDO, F. P.; ANDRADE, L. B. S.; VIDAL, M. S.; SALES, M. P. (2005)
dc.identifier.uri.fl_str_mv https://repositorio.ufrn.br/jspui/handle/1/3089
dc.identifier.issn.none.fl_str_mv 0981-9428
identifier_str_mv GOMES, C. E. M.; BARBOSA, A. E. A. D.; MACEDO, L. L. P.; PITANGA, J. C. M.; MOURA, F. T.; OLIVEIRA, A. S.; MOURA, R. M.; QUEIROZ, A. F. S.; MACEDO, F. P.; ANDRADE, L. B. S.; VIDAL, M. S.; SALES, M. P. (2005)
0981-9428
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dc.publisher.none.fl_str_mv Plant Physiology and Biochemistry
publisher.none.fl_str_mv Plant Physiology and Biochemistry
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