Detection of HPV DNA and immunohistochemical expression of cell cycle proteins in oral carcinoma in a population of brazilian patients

Detalhes bibliográficos
Autor(a) principal: Soares, Rosilene Calazans
Data de Publicação: 2008
Outros Autores: Oliveira, Márcio Campos de, Souza, Lélia Batista de, Costa, Antonio de Lisboa Lopes, Pinto, Leão Pereira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFRN
Texto Completo: https://repositorio.ufrn.br/jspui/handle/123456789/23862
Resumo: This study investigated the presence of human papillomavirus (HPV) DNA and viral types in 33 cases of oral squamous cells carcinoma (OSCC) and compared the immunohistochemical expression of the cell-cycle markers p21 and pRb between cases of the disease with and without HPV. DNA was extracted from paraffin-embedded tissue and amplified by PCR for the detection of HPV DNA. Viral typing was performed by dot blot hybridization. Immunohistochemistry was performed by the streptavidin-biotin technique. HPV DNA was detected in 11 (33.33%) of the 33 cases. The prevalent viral type was HPV 18 (81.81%). A significant association was observed between the presence of HPV and immunohistochemical expression of pRb, but not between p21 expression and the presence of the virus. The low frequency of detection of HPV DNA in OSCC suggests a possible participation of the virus in the development and progression of only a subgroup of these tumors.
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spelling Soares, Rosilene CalazansOliveira, Márcio Campos deSouza, Lélia Batista deCosta, Antonio de Lisboa LopesPinto, Leão Pereira2017-09-13T15:25:53Z2017-09-13T15:25:53Z2008SOARES, Rosilene Calazans et al . Detection of HPV DNA and immunohistochemical expression of cell cycle proteins in oral carcinoma in a population of brazilian patients. Journal of Applied Oral Science, v. 16, n. 5, p. 340-344, 2008.https://repositorio.ufrn.br/jspui/handle/123456789/2386210.1590/S1678-77572008000500007engDNA ProbesHPVCarcinomaSquamous cell cancerPolymerase Chain ReactionHybridizationCell cycleDetection of HPV DNA and immunohistochemical expression of cell cycle proteins in oral carcinoma in a population of brazilian patientsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleThis study investigated the presence of human papillomavirus (HPV) DNA and viral types in 33 cases of oral squamous cells carcinoma (OSCC) and compared the immunohistochemical expression of the cell-cycle markers p21 and pRb between cases of the disease with and without HPV. DNA was extracted from paraffin-embedded tissue and amplified by PCR for the detection of HPV DNA. Viral typing was performed by dot blot hybridization. Immunohistochemistry was performed by the streptavidin-biotin technique. HPV DNA was detected in 11 (33.33%) of the 33 cases. The prevalent viral type was HPV 18 (81.81%). A significant association was observed between the presence of HPV and immunohistochemical expression of pRb, but not between p21 expression and the presence of the virus. The low frequency of detection of HPV DNA in OSCC suggests a possible participation of the virus in the development and progression of only a subgroup of these tumors.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRNinstname:Universidade Federal do Rio Grande do Norte (UFRN)instacron:UFRNORIGINALDetectionHPVDNAImmunohistochemical_Soares_2008.pdfDetectionHPVDNAImmunohistochemical_Soares_2008.pdfhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4327600/application/pdf252969https://repositorio.ufrn.br/bitstream/123456789/23862/1/DetectionHPVDNAImmunohistochemical_Soares_2008.pdf5642b03c7fb7d070bea0ac863bca65feMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://repositorio.ufrn.br/bitstream/123456789/23862/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52TEXTDETECTION OF HPV DNA AND IMMUNOHISTOCHEMICAL.pdf.txtDETECTION OF HPV DNA AND IMMUNOHISTOCHEMICAL.pdf.txtExtracted texttext/plain20969https://repositorio.ufrn.br/bitstream/123456789/23862/5/DETECTION%20OF%20HPV%20DNA%20AND%20IMMUNOHISTOCHEMICAL.pdf.txt51e79ce52445b3f6de92aecbc03ca91bMD55THUMBNAILDETECTION OF HPV DNA AND IMMUNOHISTOCHEMICAL.pdf.jpgDETECTION OF HPV DNA AND IMMUNOHISTOCHEMICAL.pdf.jpgIM Thumbnailimage/jpeg7644https://repositorio.ufrn.br/bitstream/123456789/23862/6/DETECTION%20OF%20HPV%20DNA%20AND%20IMMUNOHISTOCHEMICAL.pdf.jpgca8bf5c7e1b1a4976221da12dff18c57MD56123456789/238622021-12-09 13:10:39.6oai:https://repositorio.ufrn.br:123456789/23862Tk9URTogUExBQ0UgWU9VUiBPV04gTElDRU5TRSBIRVJFClRoaXMgc2FtcGxlIGxpY2Vuc2UgaXMgcHJvdmlkZWQgZm9yIGluZm9ybWF0aW9uYWwgcHVycG9zZXMgb25seS4KCk5PTi1FWENMVVNJVkUgRElTVFJJQlVUSU9OIExJQ0VOU0UKCkJ5IHNpZ25pbmcgYW5kIHN1Ym1pdHRpbmcgdGhpcyBsaWNlbnNlLCB5b3UgKHRoZSBhdXRob3Iocykgb3IgY29weXJpZ2h0Cm93bmVyKSBncmFudHMgdG8gRFNwYWNlIFVuaXZlcnNpdHkgKERTVSkgdGhlIG5vbi1leGNsdXNpdmUgcmlnaHQgdG8gcmVwcm9kdWNlLAp0cmFuc2xhdGUgKGFzIGRlZmluZWQgYmVsb3cpLCBhbmQvb3IgZGlzdHJpYnV0ZSB5b3VyIHN1Ym1pc3Npb24gKGluY2x1ZGluZwp0aGUgYWJzdHJhY3QpIHdvcmxkd2lkZSBpbiBwcmludCBhbmQgZWxlY3Ryb25pYyBmb3JtYXQgYW5kIGluIGFueSBtZWRpdW0sCmluY2x1ZGluZyBidXQgbm90IGxpbWl0ZWQgdG8gYXVkaW8gb3IgdmlkZW8uCgpZb3UgYWdyZWUgdGhhdCBEU1UgbWF5LCB3aXRob3V0IGNoYW5naW5nIHRoZSBjb250ZW50LCB0cmFuc2xhdGUgdGhlCnN1Ym1pc3Npb24gdG8gYW55IG1lZGl1bSBvciBmb3JtYXQgZm9yIHRoZSBwdXJwb3NlIG9mIHByZXNlcnZhdGlvbi4KCllvdSBhbHNvIGFncmVlIHRoYXQgRFNVIG1heSBrZWVwIG1vcmUgdGhhbiBvbmUgY29weSBvZiB0aGlzIHN1Ym1pc3Npb24gZm9yCnB1cnBvc2VzIG9mIHNlY3VyaXR5LCBiYWNrLXVwIGFuZCBwcmVzZXJ2YXRpb24uCgpZb3UgcmVwcmVzZW50IHRoYXQgdGhlIHN1Ym1pc3Npb24gaXMgeW91ciBvcmlnaW5hbCB3b3JrLCBhbmQgdGhhdCB5b3UgaGF2ZQp0aGUgcmlnaHQgdG8gZ3JhbnQgdGhlIHJpZ2h0cyBjb250YWluZWQgaW4gdGhpcyBsaWNlbnNlLiBZb3UgYWxzbyByZXByZXNlbnQKdGhhdCB5b3VyIHN1Ym1pc3Npb24gZG9lcyBub3QsIHRvIHRoZSBiZXN0IG9mIHlvdXIga25vd2xlZGdlLCBpbmZyaW5nZSB1cG9uCmFueW9uZSdzIGNvcHlyaWdodC4KCklmIHRoZSBzdWJtaXNzaW9uIGNvbnRhaW5zIG1hdGVyaWFsIGZvciB3aGljaCB5b3UgZG8gbm90IGhvbGQgY29weXJpZ2h0LAp5b3UgcmVwcmVzZW50IHRoYXQgeW91IGhhdmUgb2J0YWluZWQgdGhlIHVucmVzdHJpY3RlZCBwZXJtaXNzaW9uIG9mIHRoZQpjb3B5cmlnaHQgb3duZXIgdG8gZ3JhbnQgRFNVIHRoZSByaWdodHMgcmVxdWlyZWQgYnkgdGhpcyBsaWNlbnNlLCBhbmQgdGhhdApzdWNoIHRoaXJkLXBhcnR5IG93bmVkIG1hdGVyaWFsIGlzIGNsZWFybHkgaWRlbnRpZmllZCBhbmQgYWNrbm93bGVkZ2VkCndpdGhpbiB0aGUgdGV4dCBvciBjb250ZW50IG9mIHRoZSBzdWJtaXNzaW9uLgoKSUYgVEhFIFNVQk1JU1NJT04gSVMgQkFTRUQgVVBPTiBXT1JLIFRIQVQgSEFTIEJFRU4gU1BPTlNPUkVEIE9SIFNVUFBPUlRFRApCWSBBTiBBR0VOQ1kgT1IgT1JHQU5JWkFUSU9OIE9USEVSIFRIQU4gRFNVLCBZT1UgUkVQUkVTRU5UIFRIQVQgWU9VIEhBVkUKRlVMRklMTEVEIEFOWSBSSUdIVCBPRiBSRVZJRVcgT1IgT1RIRVIgT0JMSUdBVElPTlMgUkVRVUlSRUQgQlkgU1VDSApDT05UUkFDVCBPUiBBR1JFRU1FTlQuCgpEU1Ugd2lsbCBjbGVhcmx5IGlkZW50aWZ5IHlvdXIgbmFtZShzKSBhcyB0aGUgYXV0aG9yKHMpIG9yIG93bmVyKHMpIG9mIHRoZQpzdWJtaXNzaW9uLCBhbmQgd2lsbCBub3QgbWFrZSBhbnkgYWx0ZXJhdGlvbiwgb3RoZXIgdGhhbiBhcyBhbGxvd2VkIGJ5IHRoaXMKbGljZW5zZSwgdG8geW91ciBzdWJtaXNzaW9uLgo=Repositório de PublicaçõesPUBhttp://repositorio.ufrn.br/oai/opendoar:2021-12-09T16:10:39Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)false
dc.title.pt_BR.fl_str_mv Detection of HPV DNA and immunohistochemical expression of cell cycle proteins in oral carcinoma in a population of brazilian patients
title Detection of HPV DNA and immunohistochemical expression of cell cycle proteins in oral carcinoma in a population of brazilian patients
spellingShingle Detection of HPV DNA and immunohistochemical expression of cell cycle proteins in oral carcinoma in a population of brazilian patients
Soares, Rosilene Calazans
DNA Probes
HPV
Carcinoma
Squamous cell cancer
Polymerase Chain Reaction
Hybridization
Cell cycle
title_short Detection of HPV DNA and immunohistochemical expression of cell cycle proteins in oral carcinoma in a population of brazilian patients
title_full Detection of HPV DNA and immunohistochemical expression of cell cycle proteins in oral carcinoma in a population of brazilian patients
title_fullStr Detection of HPV DNA and immunohistochemical expression of cell cycle proteins in oral carcinoma in a population of brazilian patients
title_full_unstemmed Detection of HPV DNA and immunohistochemical expression of cell cycle proteins in oral carcinoma in a population of brazilian patients
title_sort Detection of HPV DNA and immunohistochemical expression of cell cycle proteins in oral carcinoma in a population of brazilian patients
author Soares, Rosilene Calazans
author_facet Soares, Rosilene Calazans
Oliveira, Márcio Campos de
Souza, Lélia Batista de
Costa, Antonio de Lisboa Lopes
Pinto, Leão Pereira
author_role author
author2 Oliveira, Márcio Campos de
Souza, Lélia Batista de
Costa, Antonio de Lisboa Lopes
Pinto, Leão Pereira
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Soares, Rosilene Calazans
Oliveira, Márcio Campos de
Souza, Lélia Batista de
Costa, Antonio de Lisboa Lopes
Pinto, Leão Pereira
dc.subject.por.fl_str_mv DNA Probes
HPV
Carcinoma
Squamous cell cancer
Polymerase Chain Reaction
Hybridization
Cell cycle
topic DNA Probes
HPV
Carcinoma
Squamous cell cancer
Polymerase Chain Reaction
Hybridization
Cell cycle
description This study investigated the presence of human papillomavirus (HPV) DNA and viral types in 33 cases of oral squamous cells carcinoma (OSCC) and compared the immunohistochemical expression of the cell-cycle markers p21 and pRb between cases of the disease with and without HPV. DNA was extracted from paraffin-embedded tissue and amplified by PCR for the detection of HPV DNA. Viral typing was performed by dot blot hybridization. Immunohistochemistry was performed by the streptavidin-biotin technique. HPV DNA was detected in 11 (33.33%) of the 33 cases. The prevalent viral type was HPV 18 (81.81%). A significant association was observed between the presence of HPV and immunohistochemical expression of pRb, but not between p21 expression and the presence of the virus. The low frequency of detection of HPV DNA in OSCC suggests a possible participation of the virus in the development and progression of only a subgroup of these tumors.
publishDate 2008
dc.date.issued.fl_str_mv 2008
dc.date.accessioned.fl_str_mv 2017-09-13T15:25:53Z
dc.date.available.fl_str_mv 2017-09-13T15:25:53Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SOARES, Rosilene Calazans et al . Detection of HPV DNA and immunohistochemical expression of cell cycle proteins in oral carcinoma in a population of brazilian patients. Journal of Applied Oral Science, v. 16, n. 5, p. 340-344, 2008.
dc.identifier.uri.fl_str_mv https://repositorio.ufrn.br/jspui/handle/123456789/23862
dc.identifier.doi.none.fl_str_mv 10.1590/S1678-77572008000500007
identifier_str_mv SOARES, Rosilene Calazans et al . Detection of HPV DNA and immunohistochemical expression of cell cycle proteins in oral carcinoma in a population of brazilian patients. Journal of Applied Oral Science, v. 16, n. 5, p. 340-344, 2008.
10.1590/S1678-77572008000500007
url https://repositorio.ufrn.br/jspui/handle/123456789/23862
dc.language.iso.fl_str_mv eng
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