Detection of HPV DNA and immunohistochemical expression of cell cycle proteins in oral carcinoma in a population of brazilian patients
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of applied oral science (Online) |
Texto Completo: | https://www.revistas.usp.br/jaos/article/view/3593 |
Resumo: | This study investigated the presence of human papillomavirus (HPV) DNA and viral types in 33 cases of oral squamous cells carcinoma (OSCC) and compared the immunohistochemical expression of the cell-cycle markers p21 and pRb between cases of the disease with and without HPV. DNA was extracted from paraffin-embedded tissue and amplified by PCR for the detection of HPV DNA. Viral typing was performed by dot blot hybridization. Immunohistochemistry was performed by the streptavidinbiotin technique. HPV DNA was detected in 11 (33.33%) of the 33 cases. The prevalent viral type was HPV 18 (81.81%). A significant association was observed between the presence of HPV and immunohistochemical expression of pRb, but not between p21 expression and the presence of the virus. The low frequency of detection of HPV DNA in OSCC suggests a possible participation of the virus in the development and progression of only a subgroup of these tumors. |
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Journal of applied oral science (Online) |
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Detection of HPV DNA and immunohistochemical expression of cell cycle proteins in oral carcinoma in a population of brazilian patients DNA ProbesHPVCarcinomaSquamous cell cancerPolymerase Chain ReactionHybridizationCell cycle This study investigated the presence of human papillomavirus (HPV) DNA and viral types in 33 cases of oral squamous cells carcinoma (OSCC) and compared the immunohistochemical expression of the cell-cycle markers p21 and pRb between cases of the disease with and without HPV. DNA was extracted from paraffin-embedded tissue and amplified by PCR for the detection of HPV DNA. Viral typing was performed by dot blot hybridization. Immunohistochemistry was performed by the streptavidinbiotin technique. HPV DNA was detected in 11 (33.33%) of the 33 cases. The prevalent viral type was HPV 18 (81.81%). A significant association was observed between the presence of HPV and immunohistochemical expression of pRb, but not between p21 expression and the presence of the virus. The low frequency of detection of HPV DNA in OSCC suggests a possible participation of the virus in the development and progression of only a subgroup of these tumors. Universidade de São Paulo. Faculdade de Odontologia de Bauru2008-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/jaos/article/view/359310.1590/S1678-77572008000500007Journal of Applied Oral Science; Vol. 16 No. 5 (2008); 340-344 Journal of Applied Oral Science; Vol. 16 Núm. 5 (2008); 340-344 Journal of Applied Oral Science; v. 16 n. 5 (2008); 340-344 1678-77651678-7757reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/jaos/article/view/3593/4283Copyright (c) 2008 Journal of Applied Oral Scienceinfo:eu-repo/semantics/openAccessSoares, Rosilene CalazansOliveira, Márcio CamposSouza, Lélia Batista deCosta, Antônio de Lisboa LopesPinto, Leão Pereira2012-04-27T12:01:41Zoai:revistas.usp.br:article/3593Revistahttp://www.scielo.br/jaosPUBhttps://www.revistas.usp.br/jaos/oai||jaos@usp.br1678-77651678-7757opendoar:2012-04-27T12:01:41Journal of applied oral science (Online) - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Detection of HPV DNA and immunohistochemical expression of cell cycle proteins in oral carcinoma in a population of brazilian patients |
title |
Detection of HPV DNA and immunohistochemical expression of cell cycle proteins in oral carcinoma in a population of brazilian patients |
spellingShingle |
Detection of HPV DNA and immunohistochemical expression of cell cycle proteins in oral carcinoma in a population of brazilian patients Soares, Rosilene Calazans DNA Probes HPV Carcinoma Squamous cell cancer Polymerase Chain Reaction Hybridization Cell cycle |
title_short |
Detection of HPV DNA and immunohistochemical expression of cell cycle proteins in oral carcinoma in a population of brazilian patients |
title_full |
Detection of HPV DNA and immunohistochemical expression of cell cycle proteins in oral carcinoma in a population of brazilian patients |
title_fullStr |
Detection of HPV DNA and immunohistochemical expression of cell cycle proteins in oral carcinoma in a population of brazilian patients |
title_full_unstemmed |
Detection of HPV DNA and immunohistochemical expression of cell cycle proteins in oral carcinoma in a population of brazilian patients |
title_sort |
Detection of HPV DNA and immunohistochemical expression of cell cycle proteins in oral carcinoma in a population of brazilian patients |
author |
Soares, Rosilene Calazans |
author_facet |
Soares, Rosilene Calazans Oliveira, Márcio Campos Souza, Lélia Batista de Costa, Antônio de Lisboa Lopes Pinto, Leão Pereira |
author_role |
author |
author2 |
Oliveira, Márcio Campos Souza, Lélia Batista de Costa, Antônio de Lisboa Lopes Pinto, Leão Pereira |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Soares, Rosilene Calazans Oliveira, Márcio Campos Souza, Lélia Batista de Costa, Antônio de Lisboa Lopes Pinto, Leão Pereira |
dc.subject.por.fl_str_mv |
DNA Probes HPV Carcinoma Squamous cell cancer Polymerase Chain Reaction Hybridization Cell cycle |
topic |
DNA Probes HPV Carcinoma Squamous cell cancer Polymerase Chain Reaction Hybridization Cell cycle |
description |
This study investigated the presence of human papillomavirus (HPV) DNA and viral types in 33 cases of oral squamous cells carcinoma (OSCC) and compared the immunohistochemical expression of the cell-cycle markers p21 and pRb between cases of the disease with and without HPV. DNA was extracted from paraffin-embedded tissue and amplified by PCR for the detection of HPV DNA. Viral typing was performed by dot blot hybridization. Immunohistochemistry was performed by the streptavidinbiotin technique. HPV DNA was detected in 11 (33.33%) of the 33 cases. The prevalent viral type was HPV 18 (81.81%). A significant association was observed between the presence of HPV and immunohistochemical expression of pRb, but not between p21 expression and the presence of the virus. The low frequency of detection of HPV DNA in OSCC suggests a possible participation of the virus in the development and progression of only a subgroup of these tumors. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-10-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/jaos/article/view/3593 10.1590/S1678-77572008000500007 |
url |
https://www.revistas.usp.br/jaos/article/view/3593 |
identifier_str_mv |
10.1590/S1678-77572008000500007 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/jaos/article/view/3593/4283 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2008 Journal of Applied Oral Science info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2008 Journal of Applied Oral Science |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Odontologia de Bauru |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Odontologia de Bauru |
dc.source.none.fl_str_mv |
Journal of Applied Oral Science; Vol. 16 No. 5 (2008); 340-344 Journal of Applied Oral Science; Vol. 16 Núm. 5 (2008); 340-344 Journal of Applied Oral Science; v. 16 n. 5 (2008); 340-344 1678-7765 1678-7757 reponame:Journal of applied oral science (Online) instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Journal of applied oral science (Online) |
collection |
Journal of applied oral science (Online) |
repository.name.fl_str_mv |
Journal of applied oral science (Online) - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
||jaos@usp.br |
_version_ |
1800221673982525440 |