Detection of HPV DNA and immunohistochemical expression of cell cycle proteins in oral carcinoma in a population of brazilian patients

Detalhes bibliográficos
Autor(a) principal: Soares, Rosilene Calazans
Data de Publicação: 2008
Outros Autores: Oliveira, Márcio Campos, Souza, Lélia Batista de, Costa, Antônio de Lisboa Lopes, Pinto, Leão Pereira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Journal of applied oral science (Online)
Texto Completo: https://www.revistas.usp.br/jaos/article/view/3593
Resumo: This study investigated the presence of human papillomavirus (HPV) DNA and viral types in 33 cases of oral squamous cells carcinoma (OSCC) and compared the immunohistochemical expression of the cell-cycle markers p21 and pRb between cases of the disease with and without HPV. DNA was extracted from paraffin-embedded tissue and amplified by PCR for the detection of HPV DNA. Viral typing was performed by dot blot hybridization. Immunohistochemistry was performed by the streptavidinbiotin technique. HPV DNA was detected in 11 (33.33%) of the 33 cases. The prevalent viral type was HPV 18 (81.81%). A significant association was observed between the presence of HPV and immunohistochemical expression of pRb, but not between p21 expression and the presence of the virus. The low frequency of detection of HPV DNA in OSCC suggests a possible participation of the virus in the development and progression of only a subgroup of these tumors.
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spelling Detection of HPV DNA and immunohistochemical expression of cell cycle proteins in oral carcinoma in a population of brazilian patients DNA ProbesHPVCarcinomaSquamous cell cancerPolymerase Chain ReactionHybridizationCell cycle This study investigated the presence of human papillomavirus (HPV) DNA and viral types in 33 cases of oral squamous cells carcinoma (OSCC) and compared the immunohistochemical expression of the cell-cycle markers p21 and pRb between cases of the disease with and without HPV. DNA was extracted from paraffin-embedded tissue and amplified by PCR for the detection of HPV DNA. Viral typing was performed by dot blot hybridization. Immunohistochemistry was performed by the streptavidinbiotin technique. HPV DNA was detected in 11 (33.33%) of the 33 cases. The prevalent viral type was HPV 18 (81.81%). A significant association was observed between the presence of HPV and immunohistochemical expression of pRb, but not between p21 expression and the presence of the virus. The low frequency of detection of HPV DNA in OSCC suggests a possible participation of the virus in the development and progression of only a subgroup of these tumors. Universidade de São Paulo. Faculdade de Odontologia de Bauru2008-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/jaos/article/view/359310.1590/S1678-77572008000500007Journal of Applied Oral Science; Vol. 16 No. 5 (2008); 340-344 Journal of Applied Oral Science; Vol. 16 Núm. 5 (2008); 340-344 Journal of Applied Oral Science; v. 16 n. 5 (2008); 340-344 1678-77651678-7757reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/jaos/article/view/3593/4283Copyright (c) 2008 Journal of Applied Oral Scienceinfo:eu-repo/semantics/openAccessSoares, Rosilene CalazansOliveira, Márcio CamposSouza, Lélia Batista deCosta, Antônio de Lisboa LopesPinto, Leão Pereira2012-04-27T12:01:41Zoai:revistas.usp.br:article/3593Revistahttp://www.scielo.br/jaosPUBhttps://www.revistas.usp.br/jaos/oai||jaos@usp.br1678-77651678-7757opendoar:2012-04-27T12:01:41Journal of applied oral science (Online) - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Detection of HPV DNA and immunohistochemical expression of cell cycle proteins in oral carcinoma in a population of brazilian patients
title Detection of HPV DNA and immunohistochemical expression of cell cycle proteins in oral carcinoma in a population of brazilian patients
spellingShingle Detection of HPV DNA and immunohistochemical expression of cell cycle proteins in oral carcinoma in a population of brazilian patients
Soares, Rosilene Calazans
DNA Probes
HPV
Carcinoma
Squamous cell cancer
Polymerase Chain Reaction
Hybridization
Cell cycle
title_short Detection of HPV DNA and immunohistochemical expression of cell cycle proteins in oral carcinoma in a population of brazilian patients
title_full Detection of HPV DNA and immunohistochemical expression of cell cycle proteins in oral carcinoma in a population of brazilian patients
title_fullStr Detection of HPV DNA and immunohistochemical expression of cell cycle proteins in oral carcinoma in a population of brazilian patients
title_full_unstemmed Detection of HPV DNA and immunohistochemical expression of cell cycle proteins in oral carcinoma in a population of brazilian patients
title_sort Detection of HPV DNA and immunohistochemical expression of cell cycle proteins in oral carcinoma in a population of brazilian patients
author Soares, Rosilene Calazans
author_facet Soares, Rosilene Calazans
Oliveira, Márcio Campos
Souza, Lélia Batista de
Costa, Antônio de Lisboa Lopes
Pinto, Leão Pereira
author_role author
author2 Oliveira, Márcio Campos
Souza, Lélia Batista de
Costa, Antônio de Lisboa Lopes
Pinto, Leão Pereira
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Soares, Rosilene Calazans
Oliveira, Márcio Campos
Souza, Lélia Batista de
Costa, Antônio de Lisboa Lopes
Pinto, Leão Pereira
dc.subject.por.fl_str_mv DNA Probes
HPV
Carcinoma
Squamous cell cancer
Polymerase Chain Reaction
Hybridization
Cell cycle
topic DNA Probes
HPV
Carcinoma
Squamous cell cancer
Polymerase Chain Reaction
Hybridization
Cell cycle
description This study investigated the presence of human papillomavirus (HPV) DNA and viral types in 33 cases of oral squamous cells carcinoma (OSCC) and compared the immunohistochemical expression of the cell-cycle markers p21 and pRb between cases of the disease with and without HPV. DNA was extracted from paraffin-embedded tissue and amplified by PCR for the detection of HPV DNA. Viral typing was performed by dot blot hybridization. Immunohistochemistry was performed by the streptavidinbiotin technique. HPV DNA was detected in 11 (33.33%) of the 33 cases. The prevalent viral type was HPV 18 (81.81%). A significant association was observed between the presence of HPV and immunohistochemical expression of pRb, but not between p21 expression and the presence of the virus. The low frequency of detection of HPV DNA in OSCC suggests a possible participation of the virus in the development and progression of only a subgroup of these tumors.
publishDate 2008
dc.date.none.fl_str_mv 2008-10-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/jaos/article/view/3593
10.1590/S1678-77572008000500007
url https://www.revistas.usp.br/jaos/article/view/3593
identifier_str_mv 10.1590/S1678-77572008000500007
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/jaos/article/view/3593/4283
dc.rights.driver.fl_str_mv Copyright (c) 2008 Journal of Applied Oral Science
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2008 Journal of Applied Oral Science
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Odontologia de Bauru
publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Odontologia de Bauru
dc.source.none.fl_str_mv Journal of Applied Oral Science; Vol. 16 No. 5 (2008); 340-344
Journal of Applied Oral Science; Vol. 16 Núm. 5 (2008); 340-344
Journal of Applied Oral Science; v. 16 n. 5 (2008); 340-344
1678-7765
1678-7757
reponame:Journal of applied oral science (Online)
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Journal of applied oral science (Online)
collection Journal of applied oral science (Online)
repository.name.fl_str_mv Journal of applied oral science (Online) - Universidade de São Paulo (USP)
repository.mail.fl_str_mv ||jaos@usp.br
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