Sonic hedgehog signaling regulates mode of cell division of early cerebral cortex progenitors and increases
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRN |
Texto Completo: | https://repositorio.ufrn.br/jspui/handle/1/11786 |
Resumo: | The morphogen Sonic Hedgehog (SHH) plays a critical role in the development of different tissues. In the central nervous system, SHH is well known to contribute to the patterning of the spinal cord and separation of the brain hemispheres. In addition, it has recently been shown that SHH signaling also contributes to the patterning of the telencephalon and establishment of adult neurogenic niches. In this work, we investigated whether SHH signaling influences the behavior of neural progenitors isolated from the dorsal telencephalon, which generate excitatory neurons and macroglial cells in vitro. We observed that SHH increases proliferation of cortical progenitors and generation of astrocytes, whereas blocking SHH signaling with cyclopamine has opposite effects. In both cases, generation of neurons did not seem to be affected. However, cell survival was broadly affected by blockade of SHH signaling. SHH effects were related to three different cell phenomena: mode of cell division, cell cycle length and cell growth. Together, our data in vitro demonstrate that SHH signaling controls cell behaviors that are important for proliferation of cerebral cortex progenitors, as well as differentiation and survival of neurons and astroglial cells. |
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Araújo, Geissy L. L.Araújo, Jessica A. M.Shroeder, TimTort, Adriano Bretanha LopesCosta, Marcos Romualdo2014-03-13T16:35:20Z2014-03-13T16:35:20Z2014-03Araújo, Geissy L. L. et. al. Sonic hedgehog signaling regulates mode of cell division of early cerebral cortex progenitors and increases astrogliogenesis - See more at: http://journal.frontiersin.org/Journal/10.3389/fncel.2014.00077/abstract#sthash.R5qHdeXr.dpuf. Frontiers in Neuroscience, mar. 2014.1662-5102https://repositorio.ufrn.br/jspui/handle/1/11786The morphogen Sonic Hedgehog (SHH) plays a critical role in the development of different tissues. In the central nervous system, SHH is well known to contribute to the patterning of the spinal cord and separation of the brain hemispheres. In addition, it has recently been shown that SHH signaling also contributes to the patterning of the telencephalon and establishment of adult neurogenic niches. In this work, we investigated whether SHH signaling influences the behavior of neural progenitors isolated from the dorsal telencephalon, which generate excitatory neurons and macroglial cells in vitro. We observed that SHH increases proliferation of cortical progenitors and generation of astrocytes, whereas blocking SHH signaling with cyclopamine has opposite effects. In both cases, generation of neurons did not seem to be affected. However, cell survival was broadly affected by blockade of SHH signaling. SHH effects were related to three different cell phenomena: mode of cell division, cell cycle length and cell growth. Together, our data in vitro demonstrate that SHH signaling controls cell behaviors that are important for proliferation of cerebral cortex progenitors, as well as differentiation and survival of neurons and astroglial cells.engcerebral cortex developmentprogenitor cellsonic hedgehog (SHH)mode of cell divisionneurogenesisgliogenesiscell survivalastrocytesSonic hedgehog signaling regulates mode of cell division of early cerebral cortex progenitors and increasesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRNinstname:Universidade Federal do Rio Grande do Norte (UFRN)instacron:UFRNORIGINALMarcosCosta_ICE_Sonic_hedgehog.pdfMarcosCosta_ICE_Sonic_hedgehog.pdfapplication/pdf2664454https://repositorio.ufrn.br/bitstream/1/11786/1/MarcosCosta_ICE_Sonic_hedgehog.pdfec78bd1ca600dd899589c003de611fdaMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://repositorio.ufrn.br/bitstream/1/11786/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52TEXTMarcosCosta_ICE_Sonic_hedgehog.pdf.txtMarcosCosta_ICE_Sonic_hedgehog.pdf.txtExtracted texttext/plain44925https://repositorio.ufrn.br/bitstream/1/11786/7/MarcosCosta_ICE_Sonic_hedgehog.pdf.txt6bc5b0f8377d73659266fe88cde6b5f5MD57THUMBNAILMarcosCosta_ICE_Sonic_hedgehog.pdf.jpgMarcosCosta_ICE_Sonic_hedgehog.pdf.jpgIM Thumbnailimage/jpeg12360https://repositorio.ufrn.br/bitstream/1/11786/8/MarcosCosta_ICE_Sonic_hedgehog.pdf.jpgaa0f4c50338b8ba1537b301dfb0bb2f1MD581/117862021-07-09 17:30:43.151oai:https://repositorio.ufrn.br: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Repositório de PublicaçõesPUBhttp://repositorio.ufrn.br/oai/opendoar:2021-07-09T20:30:43Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)false |
dc.title.pt_BR.fl_str_mv |
Sonic hedgehog signaling regulates mode of cell division of early cerebral cortex progenitors and increases |
title |
Sonic hedgehog signaling regulates mode of cell division of early cerebral cortex progenitors and increases |
spellingShingle |
Sonic hedgehog signaling regulates mode of cell division of early cerebral cortex progenitors and increases Araújo, Geissy L. L. cerebral cortex development progenitor cell sonic hedgehog (SHH) mode of cell division neurogenesis gliogenesis cell survival astrocytes |
title_short |
Sonic hedgehog signaling regulates mode of cell division of early cerebral cortex progenitors and increases |
title_full |
Sonic hedgehog signaling regulates mode of cell division of early cerebral cortex progenitors and increases |
title_fullStr |
Sonic hedgehog signaling regulates mode of cell division of early cerebral cortex progenitors and increases |
title_full_unstemmed |
Sonic hedgehog signaling regulates mode of cell division of early cerebral cortex progenitors and increases |
title_sort |
Sonic hedgehog signaling regulates mode of cell division of early cerebral cortex progenitors and increases |
author |
Araújo, Geissy L. L. |
author_facet |
Araújo, Geissy L. L. Araújo, Jessica A. M. Shroeder, Tim Tort, Adriano Bretanha Lopes Costa, Marcos Romualdo |
author_role |
author |
author2 |
Araújo, Jessica A. M. Shroeder, Tim Tort, Adriano Bretanha Lopes Costa, Marcos Romualdo |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Araújo, Geissy L. L. Araújo, Jessica A. M. Shroeder, Tim Tort, Adriano Bretanha Lopes Costa, Marcos Romualdo |
dc.subject.por.fl_str_mv |
cerebral cortex development progenitor cell sonic hedgehog (SHH) mode of cell division neurogenesis gliogenesis cell survival astrocytes |
topic |
cerebral cortex development progenitor cell sonic hedgehog (SHH) mode of cell division neurogenesis gliogenesis cell survival astrocytes |
description |
The morphogen Sonic Hedgehog (SHH) plays a critical role in the development of different tissues. In the central nervous system, SHH is well known to contribute to the patterning of the spinal cord and separation of the brain hemispheres. In addition, it has recently been shown that SHH signaling also contributes to the patterning of the telencephalon and establishment of adult neurogenic niches. In this work, we investigated whether SHH signaling influences the behavior of neural progenitors isolated from the dorsal telencephalon, which generate excitatory neurons and macroglial cells in vitro. We observed that SHH increases proliferation of cortical progenitors and generation of astrocytes, whereas blocking SHH signaling with cyclopamine has opposite effects. In both cases, generation of neurons did not seem to be affected. However, cell survival was broadly affected by blockade of SHH signaling. SHH effects were related to three different cell phenomena: mode of cell division, cell cycle length and cell growth. Together, our data in vitro demonstrate that SHH signaling controls cell behaviors that are important for proliferation of cerebral cortex progenitors, as well as differentiation and survival of neurons and astroglial cells. |
publishDate |
2014 |
dc.date.accessioned.fl_str_mv |
2014-03-13T16:35:20Z |
dc.date.available.fl_str_mv |
2014-03-13T16:35:20Z |
dc.date.issued.fl_str_mv |
2014-03 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Araújo, Geissy L. L. et. al. Sonic hedgehog signaling regulates mode of cell division of early cerebral cortex progenitors and increases astrogliogenesis - See more at: http://journal.frontiersin.org/Journal/10.3389/fncel.2014.00077/abstract#sthash.R5qHdeXr.dpuf. Frontiers in Neuroscience, mar. 2014. |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufrn.br/jspui/handle/1/11786 |
dc.identifier.issn.none.fl_str_mv |
1662-5102 |
identifier_str_mv |
Araújo, Geissy L. L. et. al. Sonic hedgehog signaling regulates mode of cell division of early cerebral cortex progenitors and increases astrogliogenesis - See more at: http://journal.frontiersin.org/Journal/10.3389/fncel.2014.00077/abstract#sthash.R5qHdeXr.dpuf. Frontiers in Neuroscience, mar. 2014. 1662-5102 |
url |
https://repositorio.ufrn.br/jspui/handle/1/11786 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
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