Validação de biomarcadores de senescência em Células-Tronco Mesenquimais humanas

• Autor(a) principal: Silva, Kamilla Karla da
• Data de Publicação: 2018
• Tipo de documento: Trabalho de conclusão de curso
• Idioma: por
• Título da fonte: Repositório Institucional da UFRN
• Texto Completo: https://repositorio.ufrn.br/handle/123456789/47255
• topic: Senescência; Instabilidade genômica; SASP; Células-tronco mesenquimais humanas; Biomarcadores; CNPQ::CIENCIAS BIOLOGICAS::GENETICA::GENETICA HUMANA E MEDICA

Human mesenchymal stem cells (hMSCs) are cells with multipotent potential, capable of differentiating in vitro in osteocyte, adipocyte and chondrocyte line, adhesion capacity on plastic surfaces and the presence of distinct specific markers. These cells have been extensively studied, both associated with biomaterials, and in studies that seek to understand important processes such as cellular senescence that has been gaining ground in research. The interest is mainly due to the strong relationship between aging and the emergence of complex diseases such as cancer and degenerative diseases, the important role of cellular senescence in the physiological and morphological changes of cells cultured in vitro and the possibility of comparison with senescence in vivo. Previous results from our research group have found the differential expression of genes throughout a culture of hMSCs extracted from umbilical cord eleven genes were chosen and the kinetics of expression of all were assessed via RT-qPCR analysis . For this, CTMh of three donors were cultured from passage 6 to 20 with P8, P12, P16 and P20 being used for morphological analysis of cells and gene expression. Among the genes studied, four (THBS1, ANKRD1, IGFBP5 and LAMA5) were significantly more expressed in P20 and were therefore indicated as new biomarkers of cellular senescence in umbilical cord of hMSCs. Four genes (ANKRD1, IGFBP5, THBS1 and LAMA5) were validated as new senescence biomarkers, with the first presenting differential expression at passage 16 and the other three being expressed only at passage 20. There was no significant difference between the kinetics of expression of none of the four genes mentioned above, pointing, in turn, to the change of passage as responsible for the observed differences. It was also possible to elucidate the association of each one with the presented morphological characteristics and their relations with the profile produced by the secretory phenotype associated with senescence (SASP).