Aplica??o da biotecnologia ao estudo de lignifica??o em esp?cies lenhosas

• Autor(a) principal: Deus, Desiane Amaral de
• Data de Publicação: 2010
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Biblioteca Digital de Teses e Dissertações da UFRRJ
• Texto Completo: https://tede.ufrrj.br/jspui/handle/tede/486
• topic: lophanthera lactescens; Eeucalyptus spp; lignifica??o; cultura de tecido; infravermelho; lophanthera lactescens; eucalyptus; lignification, tissue culture; infrared; Recursos Florestais e Engenharia Florestal

Wood is the fifth most consumed product on the world market and has the expectation of consumption growth of 20% for the next decade. Their structural constitution is formed by cellulose, hemicellulose and lignin. Woody plants, in general, have a high lignin content, reaching values above 35% compared to its dry weight. This research aimed to explore the process of lignification in callus culture obtained from Eucalyptus grandis, E. urophylla hybrid E. urograndis and species Lophanthera lactescens Ducke and monitor this process in plants of L. lactescens cultured in vitro. The work is divided in two chapters. The first is related to the species L. lactescens and deals with the determination of a protocol for callus induction and evaluation of the process of lignification in callus and in roots and stems of plants grown in vitro. The process of lignification in plants was accompanied by anatomical characterization. The second chapter, referring to two species of Eucalyptus and the hybrid is the establishment of protocol for callus induction and assessment of the production of lignin in callus produced from different types of explants. To evaluate the process of lignification histochemical tests were made and studies of bright field microscopy, fluorescence microscopy and infrared spectrometry. The best protocol for callus induction in L. lactescens was achieved on MS medium + 9.06 mM 2,4 D. Callus from different explant sources evaluated by infrared spectroscopy showed lignin guaiacyl and syringyl type. Through the microscope and infrared spectroscopy was possible to verify that the lignification in plant L. lactescens in vitro starts at the corners of cells, tissues from young and that plants whose growth was limited by the container germination showed the occurrence of gelatinous fibers. For species of Eucalyptus, the protocol that induced a higher production of callus was MS protocol + 10 mM of 2,4-D + 2.5 mM kinetin + 20 gL-1 sucrose. The species E. urophylla callus showed larger and more friable. Callus from different sources of explants were also evaluated at the infrared signals indicative of the presence of lignin guaiacyl and syringyl type.