Lactobacillus spp de origem humana probi?ticos potenciais: caracter?sticas funcionais e de seguran?a
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| Data de Publicação: | 2015 |
| Tipo de documento: | Dissertação |
| Idioma: | por |
| Título da fonte: | Biblioteca Digital de Teses e Dissertações da UFRRJ |
| Texto Completo: | https://tede.ufrrj.br/jspui/handle/jspui/3400 |
Resumo: | The dysbiosis or imbalance of the intestinal tract microbiota becomes an increasingly common problem today, resulting in various related diseases. Bifidobacteria and lactobacilli positively influence the host health, especially due to its ability to inhibit pathogens and stimulate the immune system. The main requirements of a probiotic micro-organism is the ability to resist to digestive juices, adhesion to the intestinal epithelium to exert the activity of pathogen competitive exclusion, besides being safe. Many authors also recommend the use of species-specific probiotic to be most adaptable to the human body and therefore better competitors. This study aimed to characterize some probiotic properties (resistance to gastric and intestinal juices and antimicrobial activity) and safety aspects of probiotics (antimicrobials resistance and hemolytic capacity) of lactobacilli isolated from children infants as compared to commercial probiotic lactobacilli. In inhibition assays, no strain was able to inhibit C. albicans when grown concomitantly. However, the Lac 11 and A1 strains have proven to be as or more efficient than commercial strains to inhibit E. coli. However, the Lac 8 strain showed synergism, stimulating the growth of this bacterium. There was no significant difference in relation to antibiotic resistance between commercial and human origin strains, with the exception of strain 24, which was statistically more sensitive to ampicillin. Regarding the simulation passage through the gastrointestinal (GI) tract, there was no significant difference between the strains. Furthermore, all strains were non-hemolytic.Phenotipic identification by API 50 CHL pointed strains 24, Lac Lac 8 and 11 as belonging to the species L. rhamnosus, the strains A1 and Lac X as L. paracasei. The results indicate the potential of these strains as probiotics |
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Luchese, Rosa Helenahttp://lattes.cnpq.br/7341531211426066Cruz, Adriano Gomes daGarcia, Silvia Magalh?es Couto01973024756http://lattes.cnpq.br/7434898304676395Silva, Ivan Paulo Biano da2020-03-17T13:45:00Z2015-07-29Silva, Ivan Paulo Biano da. Lactobacillus spp de origem humana probi?ticos potenciais: caracter?sticas funcionais e de seguran?a. 2015. [56 f.]. Disserta??o( Programa de P?s-Gradua??o em Ci?ncia e Tecnologia de Alimentos) - Universidade Federal Rural do Rio de Janeiro, [Serop?dica-RJ] .https://tede.ufrrj.br/jspui/handle/jspui/3400The dysbiosis or imbalance of the intestinal tract microbiota becomes an increasingly common problem today, resulting in various related diseases. Bifidobacteria and lactobacilli positively influence the host health, especially due to its ability to inhibit pathogens and stimulate the immune system. The main requirements of a probiotic micro-organism is the ability to resist to digestive juices, adhesion to the intestinal epithelium to exert the activity of pathogen competitive exclusion, besides being safe. Many authors also recommend the use of species-specific probiotic to be most adaptable to the human body and therefore better competitors. This study aimed to characterize some probiotic properties (resistance to gastric and intestinal juices and antimicrobial activity) and safety aspects of probiotics (antimicrobials resistance and hemolytic capacity) of lactobacilli isolated from children infants as compared to commercial probiotic lactobacilli. In inhibition assays, no strain was able to inhibit C. albicans when grown concomitantly. However, the Lac 11 and A1 strains have proven to be as or more efficient than commercial strains to inhibit E. coli. However, the Lac 8 strain showed synergism, stimulating the growth of this bacterium. There was no significant difference in relation to antibiotic resistance between commercial and human origin strains, with the exception of strain 24, which was statistically more sensitive to ampicillin. Regarding the simulation passage through the gastrointestinal (GI) tract, there was no significant difference between the strains. Furthermore, all strains were non-hemolytic.Phenotipic identification by API 50 CHL pointed strains 24, Lac Lac 8 and 11 as belonging to the species L. rhamnosus, the strains A1 and Lac X as L. paracasei. The results indicate the potential of these strains as probioticsA disbiose ou desiquil?brio da microbiota intestinal torna-se um problema cada vez mais frequente na atualidade, tendo como consequ?ncia v?rias doen?as relacionadas. Bifidobact?rias e lactobacilos influenciam positivamente a sa?de, especialmente pela sua capacidade de inibir pat?genos e estimular o sistema imune. Os principais requisitos de um microrganismo probi?tico s?o a capacidade de resistir aos sucos digestivos, ades?o ao epit?lio intestinal para que exer?am atividade de exclus?o competitiva de pat?genos, al?m de serem seguros. Muitos autores tamb?m recomendam a utiliza??o de probi?ticos esp?cie-espec?ficos por serem mais adapt?veis ao organismo humano e, portanto, melhor competidores. Esta pesquisa teve como objetivo a caracteriza??o de algumas propriedades probi?ticas (resist?ncia aos sucos g?strico e intestinal, e atividade antimicrobiana) e aspectos de seguran?a dos probi?ticos (resist?ncia aos antimicrobianos e capacidade hemol?tica) de lactobacilos isolados de crian?as lactentes. Nos ensaios de inibi??o, nenhuma cepa mostrou-se capaz de reduzir Candida albicans quando cultivadas concomitantemente. Por?m, as cepas Lac 11 e A1 se mostraram t?o ou mais eficientes que as cepas comerciais em inibir Echerichia coli. Todavia, a cepa Lac 8 apresentou sinergismo no crescimento desta bact?ria. N?o houve diferen?a significativa com rela??o ? resist?ncia aos antibi?ticos testados entre as cepas comerciais e as de origem humana, com exce??o da cepa 24, que se mostrou estatisticamente mais sens?vel ? ampicilina. No que tange ? simula??o de passagem pelo trato gastrointestinal (TGI), tamb?m n?o houve diferen?a significativa entre as cepas testadas. Al?m disso, todas as cepas mostraram-se n?o hemol?ticas. A identifica??o fenot?pica pelo API 50 CHL apontou as cepas 24, Lac 8 e Lac 11 como pertencentes ? esp?cie Lactobacillus rhamnosus. J? as cepas A1 e Lac X foram identificadas como Lactobacillus paracasei. Os resultados indicaram o potencial probi?ticos destas cepas de acordo com os quesitos testados.Submitted by Celso Magalhaes (celsomagalhaes@ufrrj.br) on 2020-03-17T13:44:59Z No. of bitstreams: 1 2015 - Ivan Paulo Biano da Silva.pdf: 1417655 bytes, checksum: 103bc409f7f64df41757352fec7367a7 (MD5)Made available in DSpace on 2020-03-17T13:45:00Z (GMT). No. of bitstreams: 1 2015 - Ivan Paulo Biano da Silva.pdf: 1417655 bytes, checksum: 103bc409f7f64df41757352fec7367a7 (MD5) Previous issue date: 2015-07-29application/pdfhttps://tede.ufrrj.br/retrieve/13458/2015%20-%20Ivan%20Paulo%20Biano%20da%20Silva.pdf.jpghttps://tede.ufrrj.br/retrieve/16782/2015%20-%20Ivan%20Paulo%20Biano%20da%20Silva.pdf.jpghttps://tede.ufrrj.br/retrieve/23126/2015%20-%20Ivan%20Paulo%20Biano%20da%20Silva.pdf.jpghttps://tede.ufrrj.br/retrieve/29482/2015%20-%20Ivan%20Paulo%20Biano%20da%20Silva.pdf.jpghttps://tede.ufrrj.br/retrieve/35882/2015%20-%20Ivan%20Paulo%20Biano%20da%20Silva.pdf.jpghttps://tede.ufrrj.br/retrieve/42256/2015%20-%20Ivan%20Paulo%20Biano%20da%20Silva.pdf.jpghttps://tede.ufrrj.br/retrieve/48658/2015%20-%20Ivan%20Paulo%20Biano%20da%20Silva.pdf.jpghttps://tede.ufrrj.br/retrieve/55080/2015%20-%20Ivan%20Paulo%20Biano%20da%20Silva.pdf.jpgporUniversidade Federal Rural do Rio de JaneiroPrograma de P?s-Gradua??o em Ci?ncia e Tecnologia de AlimentosUFRRJBrasilInstituto de TecnologiaADLERBERTH, I.; AHRN?, S.; JOHANSSON, M-L.; MOLIN, G. A mannose-specific adherence mechanism in Lactobacillus plantarum conferring binding to the human colonic cell line HT-29. Applied and Environmental Microbiology. v. 62, n. 7, p. 2244-2251, Jul. 1996. ALMEIDA, L. B.; MARINHO, C. B.; SOUZA, C. S.; CHEIB, V. B. P. Disbiose intestinal. Revista Brasileira de Nutri??o Cl?nica, Belo Horizonte, v. 24, n. 1, p. 58- 65, dez. 2009 AMBADOYIANNIS, G.; HATZIKAMARI, M.; LITOPOULOU-TZANETAKI, E.; TZANETAKIS, N. Probiotic and technological properties of enterococci isolates from infants and cheese. Food Biotechnology, v. 18, n. 3, p. 307-325, 2005. AMMOR, Mohammed Salim; FL?REZ, Ana Bel?n; MAYO, Baltasar. Antibiotic resistance in non-enterococcal lactic acid bacteria and bifidobacteria. Food Microbiology, v. 24, n. 6, p. 559-570, 2007. ANJO, D. F. C. Alimentos funcionais em angiologia e cirurgia vascular. J Vasc Brasil, v. 3, n. 2, p. 145-154, 2004. BELICOV?, A.; MIKUL??OV?, M.; DU?INSK?, R. Probiotic potential and safety properties of Lactobacillus plantarum from Slovak Bryndza cheese. BioMed research international, v. 2013, 2013. BERNARDEAU, M.; VERNOUX, J. P.; HENRI-DUBERNET, S.; GUEGUEN, M. Safety assessment of dairy microorganisms: the Lactobacillus genus. International journal of food microbiology, v. 126, n. 3, p. 278-285, 2008. BERNET-CAMARD M. F.; Lievin V.; Brassart D.; Neeser J. R.; Servin A. L.; Hudault S. The human Lactobacillus acidophilus strain LA1 secretes a non-bacteriocin antibacterial substance(s) active in vitro and in vivo. Applied and environmental microbiology, v. 63, n. 7, p. 2747-2753, 1997. 35 BORIS S.; SUAREZ J.E.; BARB?S C. Characterization of the aggregation promoting factor from Lactobacillus gasseri, a vaginal isolate. Journal of Applied Microbiology. v. 83, p. 413-420, out 1997. BRASIL. Ag?ncia Nacional de Vigil?ncia Sanit?ria (ANVISA). Resolu??o n. 16 de 30 de abril de 1999. Aprova o Regulamento T?cnico de Procedimentos para registro de Alimentos e ou Novos Ingredientes, constante do anexo desta Portaria.. Bras?lia, ANVISA, 1999a. BRASIL. Ag?ncia Nacional de Vigil?ncia Sanit?ria (ANVISA). Resolu??o n. 17 de 30 de abril de 1999b. Regulamento T?cnico que estabelece as Diretrizes B?sicas para a Avalia??o de Risco e Seguran?a dos Alimentos.. Bras?lia, ANVISA, 1999b. BRASIL. Ag?ncia Nacional de Vigil?ncia Sanit?ria (ANVISA). Resolu??o n.18 de 30 de abril de 1999. Aprova regulamento t?cnico que estabelece as diretrizes b?sicas para an?lise e comprova??o de propriedades funcionais e/ou de sa?de alegadas em rotulagem de alimentos. Bras?lia, ANVISA, 1999c. BRASIL. Ag?ncia Nacional de Vigil?ncia Sanit?ria (ANVISA). n.19 de 30 de abril de 1999. Aprova regulamento t?cnico de procedimentos para registro de alimento com alega??o de propriedades funcionais e ou de sa?de em sua rotulagem. Bras?lia, ANVISA, 1999d. BRASIL. Ag?ncia Nacional de Vigil?ncia Sanit?ria (ANVISA). Resolu??o RDC n? 2, de 07 de janeiro de 2002. Aprova o Regulamento T?cnico de Subst?ncias Bioativas e Probi?ticos Isolados com Alega??o de Propriedades Funcional e ou de Sa?de. Bras?lia, ANVISA, 2002. BRASIL. Lista de alega??es de propriedades funcionais aprovada. In: Alimentos com Alega??es de Propriedades Funcionais e ou de Sa?de, Novos Alimentos/Ingredientes, Sust?ncias Bioativas e Probi?ticos. Dispon?vel em: http://portal.anvisa.gov.br/wps/content/Anvisa+Portal/Anvisa/Inicio/Alimentos/Assunto s+de+Interesse/Alimentos+Com+Alegacoes+de+Propriedades+Funcionais+e+ou+de+S aude/Alegacoes+de+propriedade+funcional+aprovadas acessado em 15/ 09/2015 36 BUJ??KOV?, D.; KME?, V. Functional properties of Lactobacillus strains isolated from dairy products. Folia microbiologica, v. 57, n. 4, p. 263-267, 2012. B?RIGO, T.; FAGUNDES, R. L. M.; TRINDADE, E. B. S. M.; VASCONCELOS, H. C. F. F. Efeito bifidog?nico do frutooligossacar?deo na microbiota intestinal de pacientes com neoplasia hematol?gica. Rev. Nutr. 20 (5) Set.-Out. 2007. CABALLERO-FRANCO, C.; Keller, K.; De Simone, C.; Chadee, K. The VSL#3 probiotic formula induces mucin gene expression and secretion in colonic epithelial cells. Mucosal Biology. v. 292(1), p. 315-322. 2007. CLEMENTI, F.; AQUILANTI, L. Recent investigations and updated criteria for the assessment of antibiotic resistance in food lactic acid bacteria. Anaerobe, v. 17, n. 6, p. 394-398, 2011. COLLINS, F. M.; CARTER, P. B. Growth of salmonellae in orally infected germfree mice. Infection and immunity, v. 21, n. 1, p. 41-47, 1978. CYPRIANO, V. H. N. Propriedades Probi?ticas de Lactobacillus spp. de Origem Humana e Intera??o com Leveduras Potencialmente Patog?nicas do G?nero Candida. 2013. 40 f. Disserta??o ( Mestrado em Ci?ncia e Tecnologia de Alimentos) ? Departamento de T?cnologia de Alimentos, Universidade Federal Rural do Rio de Janeiro, Serop?dica, 2013. DALY, C.; DAVIS, R. The biotechnology of lactic acid bacteria with emphasis on applications in food safety and human health. Agricultural and Food Science, v. 7, n. 2, p. 251-265, 2008. DE BOEVER, P.; VERSTRAETE, W. Bile salt deconjugation by Lactobacillus plantarum 80 and its implication for bacterial toxicity. Journal of applied microbiology, v. 87, n. 3, p. 345-352, 1999. DUNNE, C.; O?Mahony L.; Murphy, L.; Thornton, G.; Morrissey, D.; O?Halloran, S.; Feeney, M.; Flynn, S.; Fitzgerald, G.; Daly, C.; Kiely, B.; O?Sullivan, G. C.; Shanahan, 37 F.; Collins, J. K. In vitro selection criteria for probiotic bacteria of human origin: correlation with in vivo findings. Am J Clin Nutr 73:386S?392S, 2001. DRAGO, L.; GISTMONDO, M. R.; LOMBARDI, A.; HAEN, C.; GOZZINI, L. Inhibition of in vitro growth of enteropathogens by new Lactobacillus isolates of human intestinal origin. Federation of European Microbiological Societies. Microbiology Letters, v. 153, n. 2, p. 455-463, ago. 1997. DU?KOV?, M.; REN?TA, K. Antimicrobial resistance of lactobacilli isolated from food. Czech J Food Sci, v. 31, p. 27-32, 2013. WHO, FAO. Evaluation of health and nutritional properties of powder milk and live lactic acid bacteria. Food and Agriculture Organization of the United Nations and World Health Organization Expert Consultation Report, p. 1-34, 2001. Dispon?vel em www.fao.org/es/esn/food/prebioreport_en.pdf. Acesso em 06.09.2015 FAVARIN, L.; LAUREANO-MELO, R.; LUCHESE, R. H. Survival of free and microencapsulated Bifidobacterium: effect of honey addition. Journal of microencapsulation, n. 0, p. 1-7, 2015. FEY, P. D.; Safranek, T. J.; Rupp, M. E.; Dunne, E. F.; Ribot, E.; Iwen, P. C.; Hinrichs, S. H. Ceftriaxone-resistant Salmonella infection acquired by a child from cattle. New England Journal of Medicine, v. 342, n. 17, p. 1242-1249, 2000 FONTANA, L.; BERMUDEZ-BRITO, M.; PLAZA-DIAZ, J.; MUNOZ-QUEZADA, S.; Gil, A. Sources, isolation, characterisation and evaluation of probiotics. British journal of nutrition, v. 109, n. S2, p. S35-S50, 2013. FOOKS, L.J.; FULLER, R.; GIBSON, G.R. Probiotics, prebiotics and human gut microbiology. Int Dairy J v. 9, p. 53?61, 1999. FULLER, R. Probiotics in man and animals., v. 66, p. 365-78, 1989. 38 GIBSON, G.R.; BEATTY, E.R.; WANG, X.; COMMINGS, J.H. Selective stimulation of bifidobacteria in the human colon by oligofructose and inulin. Gastroenterology. v. 108, n. 4, p. 975-982, abr. 1995. HAENEL, H.; BENDIG, J. Intestinal flora in health and disease. Progr. Food Nutr. Sci. v. 1, p. 21- 64, 1975. HORO?OV?, K.; BUJ??KOV?, D.; KME?, V. Effect of lactobacilli on E. coli adhesion to caco-2 cellsin Vitro. Folia microbiologica, v. 51, n. 4, p. 281-282, 2006. HUMMEL, A. S.; HERTEL, C.; HOLZAPFEL, W. H.; FRANZ, C. M. Antibiotic resistances of starter and probiotic strains of lactic acid bacteria. Applied and environmental microbiology, v. 73, n. 3, p. 730-739, 2007. HYACINTA, M?jekov? et al. Bile tolerance and its effect on antibiotic susceptibility of probiotic Lactobacillus candidates. Folia microbiologica, p. 1-5, 2014. LEBEER, S.; VANDERLEYDEN, J.; DE KEERSMAECKER, S. C. J. Genes and molecules of lactobacilli supporting probiotic action.Microbiology and Molecular Biology Reviews, v. 72, n. 4, p. 728-764, 2008. LUCHESE, R. H. MICROBIAL INTERACTIONS IN THE GUT: THE ROLE OF BIOACTIVE COMPONENTS IN MILK AND HONEY. Probiotics. v.2, p. 400-416, 2012. link para acessowww.intechopen.com/articles/show/title/microbial-interactionsin-the-gut-the-role-of-bioactive-components-in-milk-and-honey LUCHESE, R.H.; MACEDO, L. N.; OLIVEIRA, G.S. Alimentos funcionais microbianos. In: Tend?ncias e inova??es em ci?ncia, tecnologia e engenharia de alimentos. Ed. Kurozawa, L.E & Costa, S.R.R editora Atheneu, S?o Paulo. Se??o lV, Cap. 11, p. 199-213, 2014. MACEDO, L. N.; LUCHESE, R. H.; GUERRA, A. F.; BARBOSA, C. G. Efeito prebi?tico do mel sobre o crescimento e Viabilidade de Bifidobacterium spp e 39 Lactobacillus spp em leite Ciencia e Tecnol de Aliment, v. 28, n.4., Campinas, S. P., oct/dez, 2008 MARTEAU, P.; GERHARDT, M. F.; MYARA, A.; BOUVIER, E.; TRIVIN, F.; RAMBAUD, J.C. Metabolism of bile salts by alimentary bacteria during transit in the human small intestine. Microb. Ecol. Health Dis. v. 8, p. 151 ? 157, 1995. Mattila-Sandholm, T.; Myll?rinen, P.; Crittenden, R.; Mogensen, G.; Fond?n, R.; Saarela, M. Technological challenges for future probiotic foods. International Dairy Journal, v. 12, n. 2, p. 173-182, 2002. METCHNIKOFF, I. The prolongation of life. Revised ed. of 1907. Heinemann, London. (s.n). 1900. MITSUOKA, T. Recent trends in research on intestinal flora. Bifidobacteria Microflora v. 1, p. 3 ? 24, 1982. MORAES, F. P.; COLLA, L. M.; Alimentos funcionais e nutrac?uticos: defini??es, legisla??o e benef?cios ? sa?de. Revista Eletr?nica de Farm?cia, v. 3, n. 2, p. 109-122, 2006. MORI M.; SAKAGAMI Y.; NARITA M. Isolation and structure of the bacterial sex pherormone CADI, that induces plasmid transfer in Streptococcus faecalis. FEBS Letters. v. 178 p. 97-100, 1985. MORRILL, J. L.; MORRILL, J. M.; FEYERHERM, A. M.; LASTER, J. F. Plasma proteins and a probiotic as ingredients in milk replacer. Journal of dairy science, v. 78, n. 4, p. 902-907, 1995. MORO, G. E.; ARSLANOGLU, S. Reproducing the bifidogenic effect of human milk in formula-fed infants: Why and how? Acta Paediatrica, v. 94, n. Supplement 449, p. 14- 17, 2005. 40 MOZZI, F.; GERBINO, E. ; FONT DE VALDEZ, G.; TORINO, M.I. Functionality of exopolysaccharides produced by lactic acid bacteria in an in vitro gastric system. Journal of Applied Microbiology, v. 107, p. 56-64, 2009. NAHAISI, M.H. Lactobacillus acidophillus: therapeutic propieties, products and enumeration. Chapte 6. Em Robinson, R.K. (ED), Developments in Foods Microbiology, pp. 153-178. Elsevier. Applied Science Publishers, Londres, RU. 1986. NOUSIAINEN, J.; SET?L?, J. Lactic acid bacterial as animal probiotics. In Lactic Acid Bacteria - Microbiology and Functional Aspects, 3rd ed. Salminen S. e Wright A. von (eds), New York: Marcel Dekker, Inc., p. 437-473, 1998. O'FLAHERTY, S.; KLAENHAMMER, T.R. The role and potential of probiotic bacteria in the gut, and the communication between gut microflora and gut/host Review Article. International Dairy Journal, v.20, n. 4, p. 262-268, 2010. OLIVEIRA, G. S. Potencial prebi?tico do leite humano comparado ao leite em p? modificado na modula??o da microbiota col?nica e sanidade de lactentes. 2011. Tese (Doutorado em Ci?ncias) ? Instituto de Tecnologia, Universidade Federal Rural do Rio de Janeiro, Serop?dica, 2011. PARKER, R.B. Probiotics, the other half of the antibiotic story. Anim. Nutr. Health v. 29, p. 4-8, 1974. PEREIRA, V. G.; G?MEZ, R. J. H. C. Atividade antimicrobiana de Lactobacillus acidophilus, contra microrganismos patog?nicos veiculados por alimentos. Semina: Ci?ncias Agr?rias, Londrina, v. 28, n. 2, p. 229-240, abr.-jun. 2007. PICOT, A.; LACROIX, C. Encapasulation of bifidobacteria in whey protein-based microcapsules and survival in simulated gastrointestinal condition and in yoghurt.International Dairy journal, v. 14, n.6, p. 505-515, 2004. 41 REDONDO-LOPEZ, V.; COOK, R.L.; SOBEL, J. D. Emerging role of lactobacilli in the control and maintenance of the vaginal bacterial microflora. Reviews of Infectious Diseases. v.12, p. 856-872, 1990. REID, G. Probiotics and prebiotics ? Progress and challenges. International Dairy Journal, v. 18 p.10-11, 969-75, 2008. RENIERO R.; COCCONCELLI, P.; BOTAZZI, V.; MORELLI, L. High frequency of conjugation in Lactobacillus mediated by an aggregation-promoting factor. Journal of General Microbiology. 138: 763-68, 1991. RUIZ, L.; MARGOLLES, A.; S?NCHEZ, B. Bile resistance mechanisms in Lactobacillus and Bifidobacterium. Frontiers in microbiology, v. 4, 2013. SAAD, S. M. I. Probi?ticos e prebi?ticos: o estado da arte. Brazilian Journal of Pharmaceutical Sciences, S?o Paulo. v. 42, n.1, p. 1-16, 2006. SAARELA, M.; MONGENSEN, G.; FOND?N, R.; MATTO, J.; MATTILASANDHOHOLN, T. Probiotic bacteria: safety, functional and technological properties. Journal of Biotechnology. v. 84 p. 197 ? 215, 2000. SALMINEN, S.; OUWEHAND, A.; BENNO, Y.; LEE, Y.K. Probiotics: how should they be defined? Trends Food Sci.Technol. v. 10, p. 107 ? 110. 1999. SALMINEN, S.; OUWEHAND, A. C.; ISOLAURI, E. Clinical applications of probiotic bacteria. International Dairy Journal, v. 8, n. 5, p. 563-572, 1998a. SALMINEN, S.; VON WRIGHT, A.; MORELLI, L.; MARTEAU, P.; BRASSART, D.; DE VOS, W.M.; FONDE?N, R.; SAXELIN, M.; COLLINS, K.; MOGENSEN, G.; 42 BIRKELAND, S.-E.; MATTILA-SANDHOLM, T. Demonstration of safety of probiotics ? a review. Int. J. Food Microbiol. 44, 93?106. 1998b. SALMINEN, S.; ISOLAURI, E.; SALMINEN, E. Probiotics and stabilisation of the gut mucosal barrier. Asia Pacific J. Clin. Nutr. V. 5, p. 53 ? 56, 1996a. SANDERS, M. E. Effect of consumption of lactic cultures on human health. In: Advances in Food and Nutrition Research. v. 37, p. 67-130, 1993. SANDERS, M. E. Probiotics: considerations for human health. Nutrition Review. v.61, n.3, p. 91-100, 2003. SAVAGE, D. C. Growth phase, cellular hydrophobicity, and adhesion in vitro of lactobacilli colonizing the keratinizing gastric epithelium in the mouse. Applied and environmental microbiology, v. 58, n. 6, 1992. SEPP, E.; ?T?EPETOVA, J.; SMIDT, I.; R?TSEP, M.; K?LJALG, S.; L?IVUKENE, K.; M?NDAR, R.; JAANIM?E, L.; L?HR, HI.; NAT?S, OB.; NAABER, P. Intestinal lactoflora in Estonian and Norwegian patients with antibiotic associated diarrhea. Anaerobe, v. 17, n. 6, p. 407-409, 2011. SICILIANO, R. A.; MAZZEO, M. F. Molecular mechanisms of probiotic action: a proteomic perspective. Current opinion in microbiology, v. 15, n. 3, p. 390-396, 2012. STEFE, C. A.; ALVES, M. A. R.; RIBEIRO, R. L. Probi?ticos, prebi?ticos e simbi?ticos Artigo de revis?o. Sa?de & Ambiente em Revista. Duque de Caxias, v. 3, n.1, p. 16-33, jan.-jun,. 2008. 43 STRINGHETA, P. C.; OLIVEIRA, T. T. D.; GOMES, R. C.; AMARAL, M. D. P. H. D.; CARVALHO, A. F. D.; VILELA, M. A. P. RBCF, Rev. bras. ci?nc. farm.(Impr.), v. 43, n. 2, p. 181-194, 2007. SUGITA, T.; TOGAWA, M. Efficacy of Lactobacillus preparation Biolactis powder in children with rotavirus enteritis. Jpn J Pediatr, v. 47, p. 2755-2762, 1994. VLKOV?, E.; RADA, V.; POPEL??OV?, P.; TROJANOV?, I.; KILLER, J. Antimicrobial susceptibility of bifidobacteria isolated from gastrointestinal tract of calves. Livestock Science, v. 105, n. 1, p. 253-259, 2006. WITTE, W. Selective pressure by antibiotic use in livestock.International Journal of Antimicrobial Agents, v. 16, p. 19-24, 2000. WYNNE, A. G.; MCCARTNEYA, A. L.; BROSTOFF, J.; HUDSPITHB, B. N.; GIBSON, G. R. An in vitro assessment of the effects of broad-spectrum antibiotics on the human gut microflora and concomitant isolation of a Lactobacillus plantarum with anti-Candida activities. Anaerobe. v. 10, n. 3, p. 165-169, jun. 2004. ZIEMER, C.J.; GIBSON, G.R. An overview of probiotics, prebiotics and synbiotics in the functional food concept: perspectives and future strategies. Int. 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| dc.title.por.fl_str_mv |
Lactobacillus spp de origem humana probi?ticos potenciais: caracter?sticas funcionais e de seguran?a |
| dc.title.alternative.eng.fl_str_mv |
Lactobacillus spp potential probiotics human origin: functional and safety features |
| title |
Lactobacillus spp de origem humana probi?ticos potenciais: caracter?sticas funcionais e de seguran?a |
| spellingShingle |
Lactobacillus spp de origem humana probi?ticos potenciais: caracter?sticas funcionais e de seguran?a Silva, Ivan Paulo Biano da Disbiose Alimentos Funcionais Lactobacillus sp Exclus?o Competitiva Dysbiosis Functional Foods Lactobacillus sp Competitive Exclusion Ci?ncia e Tecnologia de Alimentos |
| title_short |
Lactobacillus spp de origem humana probi?ticos potenciais: caracter?sticas funcionais e de seguran?a |
| title_full |
Lactobacillus spp de origem humana probi?ticos potenciais: caracter?sticas funcionais e de seguran?a |
| title_fullStr |
Lactobacillus spp de origem humana probi?ticos potenciais: caracter?sticas funcionais e de seguran?a |
| title_full_unstemmed |
Lactobacillus spp de origem humana probi?ticos potenciais: caracter?sticas funcionais e de seguran?a |
| title_sort |
Lactobacillus spp de origem humana probi?ticos potenciais: caracter?sticas funcionais e de seguran?a |
| author |
Silva, Ivan Paulo Biano da |
| author_facet |
Silva, Ivan Paulo Biano da |
| author_role |
author |
| dc.contributor.advisor1.fl_str_mv |
Luchese, Rosa Helena |
| dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/7341531211426066 |
| dc.contributor.referee1.fl_str_mv |
Cruz, Adriano Gomes da |
| dc.contributor.referee2.fl_str_mv |
Garcia, Silvia Magalh?es Couto |
| dc.contributor.authorID.fl_str_mv |
01973024756 |
| dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/7434898304676395 |
| dc.contributor.author.fl_str_mv |
Silva, Ivan Paulo Biano da |
| contributor_str_mv |
Luchese, Rosa Helena Cruz, Adriano Gomes da Garcia, Silvia Magalh?es Couto |
| dc.subject.por.fl_str_mv |
Disbiose Alimentos Funcionais Lactobacillus sp Exclus?o Competitiva |
| topic |
Disbiose Alimentos Funcionais Lactobacillus sp Exclus?o Competitiva Dysbiosis Functional Foods Lactobacillus sp Competitive Exclusion Ci?ncia e Tecnologia de Alimentos |
| dc.subject.eng.fl_str_mv |
Dysbiosis Functional Foods Lactobacillus sp Competitive Exclusion |
| dc.subject.cnpq.fl_str_mv |
Ci?ncia e Tecnologia de Alimentos |
| description |
The dysbiosis or imbalance of the intestinal tract microbiota becomes an increasingly common problem today, resulting in various related diseases. Bifidobacteria and lactobacilli positively influence the host health, especially due to its ability to inhibit pathogens and stimulate the immune system. The main requirements of a probiotic micro-organism is the ability to resist to digestive juices, adhesion to the intestinal epithelium to exert the activity of pathogen competitive exclusion, besides being safe. Many authors also recommend the use of species-specific probiotic to be most adaptable to the human body and therefore better competitors. This study aimed to characterize some probiotic properties (resistance to gastric and intestinal juices and antimicrobial activity) and safety aspects of probiotics (antimicrobials resistance and hemolytic capacity) of lactobacilli isolated from children infants as compared to commercial probiotic lactobacilli. In inhibition assays, no strain was able to inhibit C. albicans when grown concomitantly. However, the Lac 11 and A1 strains have proven to be as or more efficient than commercial strains to inhibit E. coli. However, the Lac 8 strain showed synergism, stimulating the growth of this bacterium. There was no significant difference in relation to antibiotic resistance between commercial and human origin strains, with the exception of strain 24, which was statistically more sensitive to ampicillin. Regarding the simulation passage through the gastrointestinal (GI) tract, there was no significant difference between the strains. Furthermore, all strains were non-hemolytic.Phenotipic identification by API 50 CHL pointed strains 24, Lac Lac 8 and 11 as belonging to the species L. rhamnosus, the strains A1 and Lac X as L. paracasei. The results indicate the potential of these strains as probiotics |
| publishDate |
2015 |
| dc.date.issued.fl_str_mv |
2015-07-29 |
| dc.date.accessioned.fl_str_mv |
2020-03-17T13:45:00Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
| format |
masterThesis |
| status_str |
publishedVersion |
| dc.identifier.citation.fl_str_mv |
Silva, Ivan Paulo Biano da. Lactobacillus spp de origem humana probi?ticos potenciais: caracter?sticas funcionais e de seguran?a. 2015. [56 f.]. Disserta??o( Programa de P?s-Gradua??o em Ci?ncia e Tecnologia de Alimentos) - Universidade Federal Rural do Rio de Janeiro, [Serop?dica-RJ] . |
| dc.identifier.uri.fl_str_mv |
https://tede.ufrrj.br/jspui/handle/jspui/3400 |
| identifier_str_mv |
Silva, Ivan Paulo Biano da. Lactobacillus spp de origem humana probi?ticos potenciais: caracter?sticas funcionais e de seguran?a. 2015. [56 f.]. Disserta??o( Programa de P?s-Gradua??o em Ci?ncia e Tecnologia de Alimentos) - Universidade Federal Rural do Rio de Janeiro, [Serop?dica-RJ] . |
| url |
https://tede.ufrrj.br/jspui/handle/jspui/3400 |
| dc.language.iso.fl_str_mv |
por |
| language |
por |
| dc.relation.references.por.fl_str_mv |
ADLERBERTH, I.; AHRN?, S.; JOHANSSON, M-L.; MOLIN, G. A mannose-specific adherence mechanism in Lactobacillus plantarum conferring binding to the human colonic cell line HT-29. Applied and Environmental Microbiology. v. 62, n. 7, p. 2244-2251, Jul. 1996. ALMEIDA, L. B.; MARINHO, C. B.; SOUZA, C. S.; CHEIB, V. B. P. Disbiose intestinal. Revista Brasileira de Nutri??o Cl?nica, Belo Horizonte, v. 24, n. 1, p. 58- 65, dez. 2009 AMBADOYIANNIS, G.; HATZIKAMARI, M.; LITOPOULOU-TZANETAKI, E.; TZANETAKIS, N. Probiotic and technological properties of enterococci isolates from infants and cheese. Food Biotechnology, v. 18, n. 3, p. 307-325, 2005. AMMOR, Mohammed Salim; FL?REZ, Ana Bel?n; MAYO, Baltasar. Antibiotic resistance in non-enterococcal lactic acid bacteria and bifidobacteria. Food Microbiology, v. 24, n. 6, p. 559-570, 2007. ANJO, D. F. C. Alimentos funcionais em angiologia e cirurgia vascular. J Vasc Brasil, v. 3, n. 2, p. 145-154, 2004. BELICOV?, A.; MIKUL??OV?, M.; DU?INSK?, R. Probiotic potential and safety properties of Lactobacillus plantarum from Slovak Bryndza cheese. BioMed research international, v. 2013, 2013. BERNARDEAU, M.; VERNOUX, J. P.; HENRI-DUBERNET, S.; GUEGUEN, M. Safety assessment of dairy microorganisms: the Lactobacillus genus. International journal of food microbiology, v. 126, n. 3, p. 278-285, 2008. BERNET-CAMARD M. F.; Lievin V.; Brassart D.; Neeser J. R.; Servin A. L.; Hudault S. The human Lactobacillus acidophilus strain LA1 secretes a non-bacteriocin antibacterial substance(s) active in vitro and in vivo. Applied and environmental microbiology, v. 63, n. 7, p. 2747-2753, 1997. 35 BORIS S.; SUAREZ J.E.; BARB?S C. Characterization of the aggregation promoting factor from Lactobacillus gasseri, a vaginal isolate. Journal of Applied Microbiology. v. 83, p. 413-420, out 1997. BRASIL. Ag?ncia Nacional de Vigil?ncia Sanit?ria (ANVISA). Resolu??o n. 16 de 30 de abril de 1999. Aprova o Regulamento T?cnico de Procedimentos para registro de Alimentos e ou Novos Ingredientes, constante do anexo desta Portaria.. Bras?lia, ANVISA, 1999a. BRASIL. Ag?ncia Nacional de Vigil?ncia Sanit?ria (ANVISA). Resolu??o n. 17 de 30 de abril de 1999b. Regulamento T?cnico que estabelece as Diretrizes B?sicas para a Avalia??o de Risco e Seguran?a dos Alimentos.. Bras?lia, ANVISA, 1999b. BRASIL. Ag?ncia Nacional de Vigil?ncia Sanit?ria (ANVISA). Resolu??o n.18 de 30 de abril de 1999. Aprova regulamento t?cnico que estabelece as diretrizes b?sicas para an?lise e comprova??o de propriedades funcionais e/ou de sa?de alegadas em rotulagem de alimentos. Bras?lia, ANVISA, 1999c. BRASIL. Ag?ncia Nacional de Vigil?ncia Sanit?ria (ANVISA). n.19 de 30 de abril de 1999. Aprova regulamento t?cnico de procedimentos para registro de alimento com alega??o de propriedades funcionais e ou de sa?de em sua rotulagem. Bras?lia, ANVISA, 1999d. BRASIL. Ag?ncia Nacional de Vigil?ncia Sanit?ria (ANVISA). Resolu??o RDC n? 2, de 07 de janeiro de 2002. Aprova o Regulamento T?cnico de Subst?ncias Bioativas e Probi?ticos Isolados com Alega??o de Propriedades Funcional e ou de Sa?de. Bras?lia, ANVISA, 2002. BRASIL. Lista de alega??es de propriedades funcionais aprovada. In: Alimentos com Alega??es de Propriedades Funcionais e ou de Sa?de, Novos Alimentos/Ingredientes, Sust?ncias Bioativas e Probi?ticos. Dispon?vel em: http://portal.anvisa.gov.br/wps/content/Anvisa+Portal/Anvisa/Inicio/Alimentos/Assunto s+de+Interesse/Alimentos+Com+Alegacoes+de+Propriedades+Funcionais+e+ou+de+S aude/Alegacoes+de+propriedade+funcional+aprovadas acessado em 15/ 09/2015 36 BUJ??KOV?, D.; KME?, V. Functional properties of Lactobacillus strains isolated from dairy products. Folia microbiologica, v. 57, n. 4, p. 263-267, 2012. B?RIGO, T.; FAGUNDES, R. L. M.; TRINDADE, E. B. S. M.; VASCONCELOS, H. C. F. F. Efeito bifidog?nico do frutooligossacar?deo na microbiota intestinal de pacientes com neoplasia hematol?gica. Rev. Nutr. 20 (5) Set.-Out. 2007. CABALLERO-FRANCO, C.; Keller, K.; De Simone, C.; Chadee, K. The VSL#3 probiotic formula induces mucin gene expression and secretion in colonic epithelial cells. Mucosal Biology. v. 292(1), p. 315-322. 2007. CLEMENTI, F.; AQUILANTI, L. Recent investigations and updated criteria for the assessment of antibiotic resistance in food lactic acid bacteria. Anaerobe, v. 17, n. 6, p. 394-398, 2011. COLLINS, F. M.; CARTER, P. B. Growth of salmonellae in orally infected germfree mice. Infection and immunity, v. 21, n. 1, p. 41-47, 1978. CYPRIANO, V. H. N. Propriedades Probi?ticas de Lactobacillus spp. de Origem Humana e Intera??o com Leveduras Potencialmente Patog?nicas do G?nero Candida. 2013. 40 f. Disserta??o ( Mestrado em Ci?ncia e Tecnologia de Alimentos) ? Departamento de T?cnologia de Alimentos, Universidade Federal Rural do Rio de Janeiro, Serop?dica, 2013. DALY, C.; DAVIS, R. The biotechnology of lactic acid bacteria with emphasis on applications in food safety and human health. Agricultural and Food Science, v. 7, n. 2, p. 251-265, 2008. DE BOEVER, P.; VERSTRAETE, W. Bile salt deconjugation by Lactobacillus plantarum 80 and its implication for bacterial toxicity. Journal of applied microbiology, v. 87, n. 3, p. 345-352, 1999. DUNNE, C.; O?Mahony L.; Murphy, L.; Thornton, G.; Morrissey, D.; O?Halloran, S.; Feeney, M.; Flynn, S.; Fitzgerald, G.; Daly, C.; Kiely, B.; O?Sullivan, G. C.; Shanahan, 37 F.; Collins, J. K. In vitro selection criteria for probiotic bacteria of human origin: correlation with in vivo findings. Am J Clin Nutr 73:386S?392S, 2001. DRAGO, L.; GISTMONDO, M. R.; LOMBARDI, A.; HAEN, C.; GOZZINI, L. Inhibition of in vitro growth of enteropathogens by new Lactobacillus isolates of human intestinal origin. Federation of European Microbiological Societies. Microbiology Letters, v. 153, n. 2, p. 455-463, ago. 1997. DU?KOV?, M.; REN?TA, K. Antimicrobial resistance of lactobacilli isolated from food. Czech J Food Sci, v. 31, p. 27-32, 2013. WHO, FAO. Evaluation of health and nutritional properties of powder milk and live lactic acid bacteria. Food and Agriculture Organization of the United Nations and World Health Organization Expert Consultation Report, p. 1-34, 2001. Dispon?vel em www.fao.org/es/esn/food/prebioreport_en.pdf. Acesso em 06.09.2015 FAVARIN, L.; LAUREANO-MELO, R.; LUCHESE, R. H. Survival of free and microencapsulated Bifidobacterium: effect of honey addition. Journal of microencapsulation, n. 0, p. 1-7, 2015. FEY, P. D.; Safranek, T. J.; Rupp, M. E.; Dunne, E. F.; Ribot, E.; Iwen, P. C.; Hinrichs, S. H. Ceftriaxone-resistant Salmonella infection acquired by a child from cattle. New England Journal of Medicine, v. 342, n. 17, p. 1242-1249, 2000 FONTANA, L.; BERMUDEZ-BRITO, M.; PLAZA-DIAZ, J.; MUNOZ-QUEZADA, S.; Gil, A. Sources, isolation, characterisation and evaluation of probiotics. British journal of nutrition, v. 109, n. S2, p. S35-S50, 2013. FOOKS, L.J.; FULLER, R.; GIBSON, G.R. Probiotics, prebiotics and human gut microbiology. Int Dairy J v. 9, p. 53?61, 1999. FULLER, R. Probiotics in man and animals., v. 66, p. 365-78, 1989. 38 GIBSON, G.R.; BEATTY, E.R.; WANG, X.; COMMINGS, J.H. Selective stimulation of bifidobacteria in the human colon by oligofructose and inulin. Gastroenterology. v. 108, n. 4, p. 975-982, abr. 1995. HAENEL, H.; BENDIG, J. Intestinal flora in health and disease. Progr. Food Nutr. Sci. v. 1, p. 21- 64, 1975. HORO?OV?, K.; BUJ??KOV?, D.; KME?, V. Effect of lactobacilli on E. coli adhesion to caco-2 cellsin Vitro. Folia microbiologica, v. 51, n. 4, p. 281-282, 2006. HUMMEL, A. S.; HERTEL, C.; HOLZAPFEL, W. H.; FRANZ, C. M. Antibiotic resistances of starter and probiotic strains of lactic acid bacteria. Applied and environmental microbiology, v. 73, n. 3, p. 730-739, 2007. HYACINTA, M?jekov? et al. Bile tolerance and its effect on antibiotic susceptibility of probiotic Lactobacillus candidates. Folia microbiologica, p. 1-5, 2014. LEBEER, S.; VANDERLEYDEN, J.; DE KEERSMAECKER, S. C. J. Genes and molecules of lactobacilli supporting probiotic action.Microbiology and Molecular Biology Reviews, v. 72, n. 4, p. 728-764, 2008. LUCHESE, R. H. MICROBIAL INTERACTIONS IN THE GUT: THE ROLE OF BIOACTIVE COMPONENTS IN MILK AND HONEY. Probiotics. v.2, p. 400-416, 2012. link para acessowww.intechopen.com/articles/show/title/microbial-interactionsin-the-gut-the-role-of-bioactive-components-in-milk-and-honey LUCHESE, R.H.; MACEDO, L. N.; OLIVEIRA, G.S. Alimentos funcionais microbianos. In: Tend?ncias e inova??es em ci?ncia, tecnologia e engenharia de alimentos. Ed. Kurozawa, L.E & Costa, S.R.R editora Atheneu, S?o Paulo. Se??o lV, Cap. 11, p. 199-213, 2014. MACEDO, L. N.; LUCHESE, R. H.; GUERRA, A. F.; BARBOSA, C. G. Efeito prebi?tico do mel sobre o crescimento e Viabilidade de Bifidobacterium spp e 39 Lactobacillus spp em leite Ciencia e Tecnol de Aliment, v. 28, n.4., Campinas, S. P., oct/dez, 2008 MARTEAU, P.; GERHARDT, M. F.; MYARA, A.; BOUVIER, E.; TRIVIN, F.; RAMBAUD, J.C. Metabolism of bile salts by alimentary bacteria during transit in the human small intestine. Microb. Ecol. Health Dis. v. 8, p. 151 ? 157, 1995. Mattila-Sandholm, T.; Myll?rinen, P.; Crittenden, R.; Mogensen, G.; Fond?n, R.; Saarela, M. Technological challenges for future probiotic foods. International Dairy Journal, v. 12, n. 2, p. 173-182, 2002. METCHNIKOFF, I. The prolongation of life. Revised ed. of 1907. Heinemann, London. (s.n). 1900. MITSUOKA, T. Recent trends in research on intestinal flora. Bifidobacteria Microflora v. 1, p. 3 ? 24, 1982. MORAES, F. P.; COLLA, L. M.; Alimentos funcionais e nutrac?uticos: defini??es, legisla??o e benef?cios ? sa?de. Revista Eletr?nica de Farm?cia, v. 3, n. 2, p. 109-122, 2006. MORI M.; SAKAGAMI Y.; NARITA M. Isolation and structure of the bacterial sex pherormone CADI, that induces plasmid transfer in Streptococcus faecalis. FEBS Letters. v. 178 p. 97-100, 1985. MORRILL, J. L.; MORRILL, J. M.; FEYERHERM, A. M.; LASTER, J. F. Plasma proteins and a probiotic as ingredients in milk replacer. Journal of dairy science, v. 78, n. 4, p. 902-907, 1995. MORO, G. E.; ARSLANOGLU, S. Reproducing the bifidogenic effect of human milk in formula-fed infants: Why and how? Acta Paediatrica, v. 94, n. Supplement 449, p. 14- 17, 2005. 40 MOZZI, F.; GERBINO, E. ; FONT DE VALDEZ, G.; TORINO, M.I. Functionality of exopolysaccharides produced by lactic acid bacteria in an in vitro gastric system. Journal of Applied Microbiology, v. 107, p. 56-64, 2009. NAHAISI, M.H. Lactobacillus acidophillus: therapeutic propieties, products and enumeration. Chapte 6. Em Robinson, R.K. (ED), Developments in Foods Microbiology, pp. 153-178. Elsevier. Applied Science Publishers, Londres, RU. 1986. NOUSIAINEN, J.; SET?L?, J. Lactic acid bacterial as animal probiotics. In Lactic Acid Bacteria - Microbiology and Functional Aspects, 3rd ed. Salminen S. e Wright A. von (eds), New York: Marcel Dekker, Inc., p. 437-473, 1998. O'FLAHERTY, S.; KLAENHAMMER, T.R. The role and potential of probiotic bacteria in the gut, and the communication between gut microflora and gut/host Review Article. International Dairy Journal, v.20, n. 4, p. 262-268, 2010. OLIVEIRA, G. S. Potencial prebi?tico do leite humano comparado ao leite em p? modificado na modula??o da microbiota col?nica e sanidade de lactentes. 2011. Tese (Doutorado em Ci?ncias) ? Instituto de Tecnologia, Universidade Federal Rural do Rio de Janeiro, Serop?dica, 2011. PARKER, R.B. Probiotics, the other half of the antibiotic story. Anim. Nutr. Health v. 29, p. 4-8, 1974. PEREIRA, V. G.; G?MEZ, R. J. H. C. Atividade antimicrobiana de Lactobacillus acidophilus, contra microrganismos patog?nicos veiculados por alimentos. Semina: Ci?ncias Agr?rias, Londrina, v. 28, n. 2, p. 229-240, abr.-jun. 2007. PICOT, A.; LACROIX, C. Encapasulation of bifidobacteria in whey protein-based microcapsules and survival in simulated gastrointestinal condition and in yoghurt.International Dairy journal, v. 14, n.6, p. 505-515, 2004. 41 REDONDO-LOPEZ, V.; COOK, R.L.; SOBEL, J. D. Emerging role of lactobacilli in the control and maintenance of the vaginal bacterial microflora. Reviews of Infectious Diseases. v.12, p. 856-872, 1990. REID, G. Probiotics and prebiotics ? Progress and challenges. International Dairy Journal, v. 18 p.10-11, 969-75, 2008. RENIERO R.; COCCONCELLI, P.; BOTAZZI, V.; MORELLI, L. High frequency of conjugation in Lactobacillus mediated by an aggregation-promoting factor. Journal of General Microbiology. 138: 763-68, 1991. RUIZ, L.; MARGOLLES, A.; S?NCHEZ, B. Bile resistance mechanisms in Lactobacillus and Bifidobacterium. Frontiers in microbiology, v. 4, 2013. SAAD, S. M. I. Probi?ticos e prebi?ticos: o estado da arte. Brazilian Journal of Pharmaceutical Sciences, S?o Paulo. v. 42, n.1, p. 1-16, 2006. SAARELA, M.; MONGENSEN, G.; FOND?N, R.; MATTO, J.; MATTILASANDHOHOLN, T. Probiotic bacteria: safety, functional and technological properties. Journal of Biotechnology. v. 84 p. 197 ? 215, 2000. SALMINEN, S.; OUWEHAND, A.; BENNO, Y.; LEE, Y.K. Probiotics: how should they be defined? Trends Food Sci.Technol. v. 10, p. 107 ? 110. 1999. SALMINEN, S.; OUWEHAND, A. C.; ISOLAURI, E. Clinical applications of probiotic bacteria. International Dairy Journal, v. 8, n. 5, p. 563-572, 1998a. SALMINEN, S.; VON WRIGHT, A.; MORELLI, L.; MARTEAU, P.; BRASSART, D.; DE VOS, W.M.; FONDE?N, R.; SAXELIN, M.; COLLINS, K.; MOGENSEN, G.; 42 BIRKELAND, S.-E.; MATTILA-SANDHOLM, T. Demonstration of safety of probiotics ? a review. Int. J. Food Microbiol. 44, 93?106. 1998b. SALMINEN, S.; ISOLAURI, E.; SALMINEN, E. Probiotics and stabilisation of the gut mucosal barrier. Asia Pacific J. Clin. Nutr. V. 5, p. 53 ? 56, 1996a. SANDERS, M. E. Effect of consumption of lactic cultures on human health. In: Advances in Food and Nutrition Research. v. 37, p. 67-130, 1993. SANDERS, M. E. Probiotics: considerations for human health. Nutrition Review. v.61, n.3, p. 91-100, 2003. SAVAGE, D. C. Growth phase, cellular hydrophobicity, and adhesion in vitro of lactobacilli colonizing the keratinizing gastric epithelium in the mouse. Applied and environmental microbiology, v. 58, n. 6, 1992. SEPP, E.; ?T?EPETOVA, J.; SMIDT, I.; R?TSEP, M.; K?LJALG, S.; L?IVUKENE, K.; M?NDAR, R.; JAANIM?E, L.; L?HR, HI.; NAT?S, OB.; NAABER, P. Intestinal lactoflora in Estonian and Norwegian patients with antibiotic associated diarrhea. Anaerobe, v. 17, n. 6, p. 407-409, 2011. SICILIANO, R. A.; MAZZEO, M. F. Molecular mechanisms of probiotic action: a proteomic perspective. Current opinion in microbiology, v. 15, n. 3, p. 390-396, 2012. STEFE, C. A.; ALVES, M. A. R.; RIBEIRO, R. L. Probi?ticos, prebi?ticos e simbi?ticos Artigo de revis?o. Sa?de & Ambiente em Revista. Duque de Caxias, v. 3, n.1, p. 16-33, jan.-jun,. 2008. 43 STRINGHETA, P. C.; OLIVEIRA, T. T. D.; GOMES, R. C.; AMARAL, M. D. P. H. D.; CARVALHO, A. F. D.; VILELA, M. A. P. RBCF, Rev. bras. ci?nc. farm.(Impr.), v. 43, n. 2, p. 181-194, 2007. SUGITA, T.; TOGAWA, M. Efficacy of Lactobacillus preparation Biolactis powder in children with rotavirus enteritis. Jpn J Pediatr, v. 47, p. 2755-2762, 1994. VLKOV?, E.; RADA, V.; POPEL??OV?, P.; TROJANOV?, I.; KILLER, J. Antimicrobial susceptibility of bifidobacteria isolated from gastrointestinal tract of calves. Livestock Science, v. 105, n. 1, p. 253-259, 2006. WITTE, W. Selective pressure by antibiotic use in livestock.International Journal of Antimicrobial Agents, v. 16, p. 19-24, 2000. WYNNE, A. G.; MCCARTNEYA, A. L.; BROSTOFF, J.; HUDSPITHB, B. N.; GIBSON, G. R. An in vitro assessment of the effects of broad-spectrum antibiotics on the human gut microflora and concomitant isolation of a Lactobacillus plantarum with anti-Candida activities. Anaerobe. v. 10, n. 3, p. 165-169, jun. 2004. ZIEMER, C.J.; GIBSON, G.R. An overview of probiotics, prebiotics and synbiotics in the functional food concept: perspectives and future strategies. Int. Dairy J., Amsterdam, v.8, p.473-479, 1998 |
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