Patogenicidade de Pratylenchus brachyurus e severidade da murcha bacteriana quando associada a este nemat?ide em fumo
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UFRRJ |
Texto Completo: | https://tede.ufrrj.br/jspui/handle/tede/503 |
Resumo: | ?The pathogenicity of Pratylenchus brachyurus on tobacco (Nicotiana tabacum) cultivar K326 was evaluated in experiments testing different initial population densities of the nematode (Pi = 0; 1,000; 3,000; 9,000 and 27,000 eggs and larvae per plant), in eight replications. The isolate was monoxenically multiplied on carrot discs. At the end of the experiment (70 days after inoculation), the above ground part of each plant was weighed and measured and, the respective root system was processed. The reproduction rate of Pratylenchus brachyurus was estimated by extracting the nematodes of the roots and of the soil. Necroses on the roots of the tobacco plants were observed. Pratylenchus brachyurus caused well-delimited root lesions on tobacco root. None of the analyzed plants died; however plant growth was reduced. Also, it was observed a decrease of the fresh mass of roots and of the dry top weight, mainly under the highest population density (27,000 nematodes per plant). The reproduction rate was low. Besides the interaction between Pratylenchus brachyurus and Ralstonia solanacearum in two tobacco varieties, Virg?nia K326 and Burley By21 was evaluated. Test plants were inoculated with the nematode in the initial population density of 3,000 individuals (eggs and larvae) by plant and, 15 days later, the plants were inoculated with a sample of soil infested by the bacterium, in estimated population density of 106 CFU.g-1 of soil. Besides the mixed inoculation with Pratylenchus brachyurus and Ralstonia solanacearum, tobacco plants were single inoculated. The plants were monitored daily. Dates of the beginning of symptom expression were registered, as well as symptom evolution. When the plants were inoculated with Pratylenchus brachyurus and Ralstonia solanacearum, symptom of wilt that is caused by the bacterium Ralstonia solanacearum began earlier than in single inoculated plants, and they also evolved quicker, inclusive in the variety Virg?nia K326 that was considered more tolerant. In this case, the presence of Pratylenchus brachyurus, in the root system of these plants, probably favored the infection by Ralstonia solanacearum, even before the symptoms caused by the nematodes could be detected. This study was done in greenhouse and under laboratory conditions. |
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Pimentel, Joao Pedro189.414.869-04http://lattes.cnpq.br/2320010050208269069.307.317-90http://lattes.cnpq.br/9976919983025740Fernandes, Ana Rosa de Figueredo2016-04-28T14:57:35Z2010-06-102009-10-29FERNANDES, Ana Rosa de Figueredo. Patogenicidade de Pratylenchus brachyurus e severidade da murcha bacteriana quando associada a este nemat?ide em fumo. 2009. 58 f. Disserta??o (Mestrado em Fitossanidade e Biotecnologia Aplicada) - Instituto de Ci?ncias Biol?gicas e da Sa?de, Universidade Federal Rural do Rio de Janeiro, Serop?dica - RJ, 2009.https://tede.ufrrj.br/jspui/handle/tede/503?The pathogenicity of Pratylenchus brachyurus on tobacco (Nicotiana tabacum) cultivar K326 was evaluated in experiments testing different initial population densities of the nematode (Pi = 0; 1,000; 3,000; 9,000 and 27,000 eggs and larvae per plant), in eight replications. The isolate was monoxenically multiplied on carrot discs. At the end of the experiment (70 days after inoculation), the above ground part of each plant was weighed and measured and, the respective root system was processed. The reproduction rate of Pratylenchus brachyurus was estimated by extracting the nematodes of the roots and of the soil. Necroses on the roots of the tobacco plants were observed. Pratylenchus brachyurus caused well-delimited root lesions on tobacco root. None of the analyzed plants died; however plant growth was reduced. Also, it was observed a decrease of the fresh mass of roots and of the dry top weight, mainly under the highest population density (27,000 nematodes per plant). The reproduction rate was low. Besides the interaction between Pratylenchus brachyurus and Ralstonia solanacearum in two tobacco varieties, Virg?nia K326 and Burley By21 was evaluated. Test plants were inoculated with the nematode in the initial population density of 3,000 individuals (eggs and larvae) by plant and, 15 days later, the plants were inoculated with a sample of soil infested by the bacterium, in estimated population density of 106 CFU.g-1 of soil. Besides the mixed inoculation with Pratylenchus brachyurus and Ralstonia solanacearum, tobacco plants were single inoculated. The plants were monitored daily. Dates of the beginning of symptom expression were registered, as well as symptom evolution. When the plants were inoculated with Pratylenchus brachyurus and Ralstonia solanacearum, symptom of wilt that is caused by the bacterium Ralstonia solanacearum began earlier than in single inoculated plants, and they also evolved quicker, inclusive in the variety Virg?nia K326 that was considered more tolerant. In this case, the presence of Pratylenchus brachyurus, in the root system of these plants, probably favored the infection by Ralstonia solanacearum, even before the symptoms caused by the nematodes could be detected. This study was done in greenhouse and under laboratory conditions.?A patogenicidade de Pratylenchus brachyurus em fumo Virg?nia K326 foi avaliada em experimento no qual foram testadas diferentes densidades populacionais iniciais do nemat?ide (Pi = 0; 1.000; 3.000; 9.000 e 27.000 ovos e larvas por planta), em oito repeti??es. O isolado foi multiplicado axenicamente em discos de cenoura. Ao final do experimento (85 dias), as plantas tiveram sua parte a?rea pesada e medida e foi processado o sistema radicular de cada uma. O fator de reprodu??o foi estimado por extra??o dos nemat?ides das ra?zes e do solo. Necroses nas ra?zes das plantas foram observadas. Pratylenchus brachyurus causou les?es radiculares delimitadas nas ra?zes de fumo. N?o houve morte de nenhuma planta analisada, por?m a altura das plantas foi afetada e tamb?m a massa seca da parte a?rea, principalmente sob a densidade populacional mais alta, de 27.000 nemat?ides por planta. O fator de reprodu??o foi baixo. Al?m disto, avaliou-se a intera??o entre Pratylenchus brachyurus e Ralstonia solanacearum em fumo Virg?nia variedade K326 e fumo Burley variedade BY21. As plantas foram inicialmente inoculadas com o nemat?ide na densidade populacional de 3.000 ovos e larvas por planta e 15 dias ap?s, foram inoculadas com uma amostra de solo infestado pela bact?ria, na densidade populacional estimada de 106 UFC.g-1 de solo. Al?m da inocula??o mista com Pratylenchus brachyurus e Ralstonia solanacearum, testemunhas foram inoculadas isoladamente. As plantas foram monitoradas diariamente, anotando-se a data de in?cio e da evolu??o dos sintomas. Quando as plantas foram inoculadas com os Pratylenchus brachyurus e Ralstonia solanacearum, os sintomas de murcha, que s?o causados pela bact?ria se manifestaram mais cedo e tamb?m evolu?ram mais r?pido, inclusive na variedade K326 que foi considerada mais tolerante. Neste caso, provavelmente a presen?a de Pratylenchus brachyurus, no sistema radicular dessas plantas, favoreceu a infec??o por Ralstonia solanacearum, antes mesmo que os sintomas causados pelo nemat?ide pudessem ser observados. Este trabalho foi realizado em casa de vegeta??o e em laborat?rio.Made available in DSpace on 2016-04-28T14:57:35Z (GMT). No. of bitstreams: 1 Ana Rosa de Figueiredo Fernandes.pdf: 5291957 bytes, checksum: e9ba6c76361eb4a2e901846a5bb5c2dd (MD5) Previous issue date: 2009-10-29Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gicoapplication/pdfhttps://tede.ufrrj.br/retrieve/14287/2009%20-%20Ana%20Rosa%20de%20Figueiredo%20Fernandes.pdf.jpghttps://tede.ufrrj.br/retrieve/18580/2009%20-%20Ana%20Rosa%20de%20Figueiredo%20Fernandes.pdf.jpghttps://tede.ufrrj.br/retrieve/24918/2009%20-%20Ana%20Rosa%20de%20Figueiredo%20Fernandes.pdf.jpghttps://tede.ufrrj.br/retrieve/31277/2009%20-%20Ana%20Rosa%20de%20Figueiredo%20Fernandes.pdf.jpghttps://tede.ufrrj.br/retrieve/37673/2009%20-%20Ana%20Rosa%20de%20Figueiredo%20Fernandes.pdf.jpghttps://tede.ufrrj.br/retrieve/44021/2009%20-%20Ana%20Rosa%20de%20Figueiredo%20Fernandes.pdf.jpghttps://tede.ufrrj.br/retrieve/50415/2009%20-%20Ana%20Rosa%20de%20Figueiredo%20Fernandes.pdf.jpghttps://tede.ufrrj.br/retrieve/56889/2009%20-%20Ana%20Rosa%20de%20Figueiredo%20Fernandes.pdf.jpgporUniversidade Federal Rural do Rio de JaneiroPrograma de P?s-Gradua??o em Fitossanidade e Biotecnologia AplicadaUFRRJBrasilInstituto de Ci?ncias Biol?gicas e da Sa?deNicotiana tabacumnemat?ideralstonia solanacearumnicotiana tabacumroot-Lesionralstonia solanacearumParasitologiaPatogenicidade de Pratylenchus brachyurus e severidade da murcha bacteriana quando associada a este nemat?ide em fumoPathogenicity of Pratylenchus brachyurus and severity of bacterial wilt in association with this nematode in tobaccoinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UFRRJinstname:Universidade Federal Rural do Rio de Janeiro (UFRRJ)instacron:UFRRJTHUMBNAIL2009 - Ana Rosa de Figueiredo Fernandes.pdf.jpg2009 - Ana Rosa de Figueiredo Fernandes.pdf.jpgimage/jpeg2104http://localhost:8080/tede/bitstream/tede/503/17/2009+-+Ana+Rosa+de+Figueiredo+Fernandes.pdf.jpgc4715912a635b5fbde63d2a9b070733fMD517TEXT2009 - Ana Rosa de Figueiredo Fernandes.pdf.txt2009 - Ana Rosa de Figueiredo Fernandes.pdf.txttext/plain96188http://localhost:8080/tede/bitstream/tede/503/16/2009+-+Ana+Rosa+de+Figueiredo+Fernandes.pdf.txt0a7563f46625b27681d5e87947c7d272MD516ORIGINAL2009 - Ana Rosa de Figueiredo Fernandes.pdf2009 - Ana Rosa de Figueiredo Fernandes.pdfapplication/pdf5292090http://localhost:8080/tede/bitstream/tede/503/1/2009+-+Ana+Rosa+de+Figueiredo+Fernandes.pdf41fa500e15d8f94da1da1de0b59d8993MD51tede/5032020-05-19 02:55:07.756oai:localhost:tede/503Biblioteca Digital de Teses e Dissertaçõeshttps://tede.ufrrj.br/PUBhttps://tede.ufrrj.br/oai/requestbibliot@ufrrj.br||bibliot@ufrrj.bropendoar:2020-05-19T05:55:07Biblioteca Digital de Teses e Dissertações da UFRRJ - Universidade Federal Rural do Rio de Janeiro (UFRRJ)false |
dc.title.por.fl_str_mv |
Patogenicidade de Pratylenchus brachyurus e severidade da murcha bacteriana quando associada a este nemat?ide em fumo |
dc.title.alternative.eng.fl_str_mv |
Pathogenicity of Pratylenchus brachyurus and severity of bacterial wilt in association with this nematode in tobacco |
title |
Patogenicidade de Pratylenchus brachyurus e severidade da murcha bacteriana quando associada a este nemat?ide em fumo |
spellingShingle |
Patogenicidade de Pratylenchus brachyurus e severidade da murcha bacteriana quando associada a este nemat?ide em fumo Fernandes, Ana Rosa de Figueredo Nicotiana tabacum nemat?ide ralstonia solanacearum nicotiana tabacum root-Lesion ralstonia solanacearum Parasitologia |
title_short |
Patogenicidade de Pratylenchus brachyurus e severidade da murcha bacteriana quando associada a este nemat?ide em fumo |
title_full |
Patogenicidade de Pratylenchus brachyurus e severidade da murcha bacteriana quando associada a este nemat?ide em fumo |
title_fullStr |
Patogenicidade de Pratylenchus brachyurus e severidade da murcha bacteriana quando associada a este nemat?ide em fumo |
title_full_unstemmed |
Patogenicidade de Pratylenchus brachyurus e severidade da murcha bacteriana quando associada a este nemat?ide em fumo |
title_sort |
Patogenicidade de Pratylenchus brachyurus e severidade da murcha bacteriana quando associada a este nemat?ide em fumo |
author |
Fernandes, Ana Rosa de Figueredo |
author_facet |
Fernandes, Ana Rosa de Figueredo |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Pimentel, Joao Pedro |
dc.contributor.advisor1ID.fl_str_mv |
189.414.869-04 |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/2320010050208269 |
dc.contributor.authorID.fl_str_mv |
069.307.317-90 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/9976919983025740 |
dc.contributor.author.fl_str_mv |
Fernandes, Ana Rosa de Figueredo |
contributor_str_mv |
Pimentel, Joao Pedro |
dc.subject.por.fl_str_mv |
Nicotiana tabacum nemat?ide ralstonia solanacearum |
topic |
Nicotiana tabacum nemat?ide ralstonia solanacearum nicotiana tabacum root-Lesion ralstonia solanacearum Parasitologia |
dc.subject.eng.fl_str_mv |
nicotiana tabacum root-Lesion ralstonia solanacearum |
dc.subject.cnpq.fl_str_mv |
Parasitologia |
description |
?The pathogenicity of Pratylenchus brachyurus on tobacco (Nicotiana tabacum) cultivar K326 was evaluated in experiments testing different initial population densities of the nematode (Pi = 0; 1,000; 3,000; 9,000 and 27,000 eggs and larvae per plant), in eight replications. The isolate was monoxenically multiplied on carrot discs. At the end of the experiment (70 days after inoculation), the above ground part of each plant was weighed and measured and, the respective root system was processed. The reproduction rate of Pratylenchus brachyurus was estimated by extracting the nematodes of the roots and of the soil. Necroses on the roots of the tobacco plants were observed. Pratylenchus brachyurus caused well-delimited root lesions on tobacco root. None of the analyzed plants died; however plant growth was reduced. Also, it was observed a decrease of the fresh mass of roots and of the dry top weight, mainly under the highest population density (27,000 nematodes per plant). The reproduction rate was low. Besides the interaction between Pratylenchus brachyurus and Ralstonia solanacearum in two tobacco varieties, Virg?nia K326 and Burley By21 was evaluated. Test plants were inoculated with the nematode in the initial population density of 3,000 individuals (eggs and larvae) by plant and, 15 days later, the plants were inoculated with a sample of soil infested by the bacterium, in estimated population density of 106 CFU.g-1 of soil. Besides the mixed inoculation with Pratylenchus brachyurus and Ralstonia solanacearum, tobacco plants were single inoculated. The plants were monitored daily. Dates of the beginning of symptom expression were registered, as well as symptom evolution. When the plants were inoculated with Pratylenchus brachyurus and Ralstonia solanacearum, symptom of wilt that is caused by the bacterium Ralstonia solanacearum began earlier than in single inoculated plants, and they also evolved quicker, inclusive in the variety Virg?nia K326 that was considered more tolerant. In this case, the presence of Pratylenchus brachyurus, in the root system of these plants, probably favored the infection by Ralstonia solanacearum, even before the symptoms caused by the nematodes could be detected. This study was done in greenhouse and under laboratory conditions. |
publishDate |
2009 |
dc.date.issued.fl_str_mv |
2009-10-29 |
dc.date.available.fl_str_mv |
2010-06-10 |
dc.date.accessioned.fl_str_mv |
2016-04-28T14:57:35Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/masterThesis |
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masterThesis |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
FERNANDES, Ana Rosa de Figueredo. Patogenicidade de Pratylenchus brachyurus e severidade da murcha bacteriana quando associada a este nemat?ide em fumo. 2009. 58 f. Disserta??o (Mestrado em Fitossanidade e Biotecnologia Aplicada) - Instituto de Ci?ncias Biol?gicas e da Sa?de, Universidade Federal Rural do Rio de Janeiro, Serop?dica - RJ, 2009. |
dc.identifier.uri.fl_str_mv |
https://tede.ufrrj.br/jspui/handle/tede/503 |
identifier_str_mv |
FERNANDES, Ana Rosa de Figueredo. Patogenicidade de Pratylenchus brachyurus e severidade da murcha bacteriana quando associada a este nemat?ide em fumo. 2009. 58 f. Disserta??o (Mestrado em Fitossanidade e Biotecnologia Aplicada) - Instituto de Ci?ncias Biol?gicas e da Sa?de, Universidade Federal Rural do Rio de Janeiro, Serop?dica - RJ, 2009. |
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por |
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Universidade Federal Rural do Rio de Janeiro |
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UFRRJ |
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Brasil |
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Instituto de Ci?ncias Biol?gicas e da Sa?de |
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Universidade Federal Rural do Rio de Janeiro |
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