Avalia??o do efeito da superexpress?o do fator de transcri??o OsDof25 sobre o metabolismo e desenvolvimento radicular em arroz
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| Data de Publicação: | 2016 |
| Tipo de documento: | Dissertação |
| Idioma: | por |
| Título da fonte: | Biblioteca Digital de Teses e Dissertações da UFRRJ |
| Texto Completo: | https://tede.ufrrj.br/jspui/handle/jspui/5296 |
Resumo: | One of the main targets in plants of agricultural interest is the Nitrogen Use Efficiency (NUE). The study of regulatory proteins related to gene expression, specifically transcription factors, is a viable alternative for controlling quantitative traits such as NUE. The transcription factor OsDof25 is an ortholog ZmDof1 maize gene identified already controlling the metabolism of carbon (C). This study was conducted to evaluate N metabolism and C in rice lines overexpressing the transcription factor OsDof25 and morphophysiological changes generated by overexpression, aiming characteristics that lead to a better NUE. Lines showed increased activity in of nitrogen metabolism enzymes (Nitrate Reductase, Glutamine synthetase and glutamate synthase) and higher content of primary metabolites, indicating better assimilation efficiency. The lines L#9.6 and L#10.8 showed high development of roots, compared to wild type. The transformed lines also showed greater number of very fine roots, as well as increased volume, surface area and length, important features for the acquisition and search for nutrients and water. |
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Fernandes, Manlio Silvestre002.180.573-34Souza, Sonia Regina de003-927.487-03Ferraz Junior, Altamiro Souza de LimaGarrido, Rodrigo Grazinoli059.359.317-02http://lattes.cnpq.br/5205853825951525Braga, Renan Pinto2021-12-28T00:33:28Z2016-02-26BRAGA, Renan Pinto. Avalia??o do efeito da superexpress?o do fator de transcri??o OsDof25 sobre o metabolismo e desenvolvimento radicular em arroz. 2016. 34 f. Disserta??o (Mestrado em Agronomia, Ci?ncia do Solo) - Instituto de Agronomia, Universidade Federal Rural do Rio de Janeiro, Serop?dica-RJ, 2016.https://tede.ufrrj.br/jspui/handle/jspui/5296One of the main targets in plants of agricultural interest is the Nitrogen Use Efficiency (NUE). The study of regulatory proteins related to gene expression, specifically transcription factors, is a viable alternative for controlling quantitative traits such as NUE. The transcription factor OsDof25 is an ortholog ZmDof1 maize gene identified already controlling the metabolism of carbon (C). This study was conducted to evaluate N metabolism and C in rice lines overexpressing the transcription factor OsDof25 and morphophysiological changes generated by overexpression, aiming characteristics that lead to a better NUE. Lines showed increased activity in of nitrogen metabolism enzymes (Nitrate Reductase, Glutamine synthetase and glutamate synthase) and higher content of primary metabolites, indicating better assimilation efficiency. The lines L#9.6 and L#10.8 showed high development of roots, compared to wild type. The transformed lines also showed greater number of very fine roots, as well as increased volume, surface area and length, important features for the acquisition and search for nutrients and water.Um dos alvos mais visados em plantas de interesse agr?cola ? a Efici?ncia de Uso do Nitrog?nio (EUN). O estudo de prote?nas regulat?rias relacionadas a express?o g?nica, mais especificamente fatores de transcri??o, ? uma alternativa vi?vel para o controle de caracter?sticas quantitativas como a EUN. O fator de transcri??o OsDof25 ? um ort?logo do gene de milho ZmDof1 j? identificado controlando o metabolismo de carbono (C). Neste trabalho foi avaliado o metabolismo de N e C em linhagens de arroz superexpressando o fator de transcri??o OsDof25 e suas as altera??es morfofisiol?gicas, visando identificar caracter?sticas que levem a uma melhor EUN. Houve nas linhagens transformadas maior atividade de enzimas de redu??o e assimila??o de N (Nitrato Redutase, Glutamina Sintetase e Glutamato Sintase) e maior conte?do de metab?litos prim?rios, que indicam maior efici?ncia de assimila??o desse nutriente. As linhagens L#9.6 e L#10.8 apresentaram maior desenvolvimento das ra?zes quando comparadas com o tipo selvagem. As linhagens superexpressando o fator de transcri??o OsDof25 tamb?m apresentaram maior n?mero de ra?zes muito finas, bem como maior volume, ?rea de superf?cie e comprimento, caracter?sticas importantes para a aquisi??o e busca por nutrientes e ?gua.Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2021-12-28T00:33:28Z No. of bitstreams: 1 2016 - Renan Pinto Braga.pdf: 1129152 bytes, checksum: 3b4ced561581fd0390f81a29ab4768c1 (MD5)Made available in DSpace on 2021-12-28T00:33:28Z (GMT). No. of bitstreams: 1 2016 - Renan Pinto Braga.pdf: 1129152 bytes, checksum: 3b4ced561581fd0390f81a29ab4768c1 (MD5) Previous issue date: 2016-02-26CAPES - Coordena??o de Aperfei?oamento de Pessoal de N?vel Superiorapplication/pdfhttps://tede.ufrrj.br/retrieve/67792/2016%20-%20Renan%20Pinto%20Braga.pdf.jpgporUniversidade Federal Rural do Rio de JaneiroPrograma de P?s-Gradua??o em Agronomia - Ci?ncia do SoloUFRRJBrasilInstituto de AgronomiaBAUMANN, K., GUALBERTI, G. The DNA-binding site of the Dof protein NtBBF1 is essential for tissue-specific and auxin-regulated expression of the rolB oncogene in plants. Plant Cell n.11, p.323-333, 1999. BARBER, S. A. Soil nutrient bioavailability: a mechanistic approach. John Wiley & Sons, 1995. BERNARD, S. M., M?LLER, A.L., DIONISIO, G., KICHEY, T., JAHN, T.P., DUBOIS, F., BAUDO, M., LOPES, M.S., TERC?-LAFORGUE, T., FOYER, C.H., PARRY, M.A., FORDE, B.G., ARAUS, J.L., HIREL, B., SCHJOERRING, J.K., HABASH, D.Z. Gene expression, cellular localisation and function of glutamine synthetase isozymes in wheat (Triticum aestivum L.). Plant Molecular Biology, v. 67, n. 1-2, p. 89-105, 2008. BRADFORD, M.M. Rapid and sensitive method for quantification of microgram quantities of protein utilizing the principle of protein dye-binding. Analytical Biochemistry, v.72, p.248-254, 1976. CAI, H., ZHOU, Y., XIAO, J., LI, X., ZHANG, Q., LIAN, X., Overexpressed glutamine synthetase gene modifies nitrogen metabolism and abiotic stress responses in rice. Plant Cell Reports, v. 28, n. 3, p. 527-537, 2009. CHASTAIN, C. J., FAILING, C. J., MANANDHAR, L., ZIMMERMAN, M. A., LAKNER, M. M., NGUYEN, TONY. H. Functional evolution of C4 pyruvate, orthophosphate dikinase. Journal of Experimental Botany, v. 62, n. 9, p. 3083?3091, 2011. CHEN, F. L., BENNETT, M. J., CULLIMORE, J. V. Effect of the Nitrogen Supply on the Activities of Isoenzymes of NADH-dependent Glutamate Synthase and Glutamine Synthetase in Root Nodules of Phaseolus vulgaris L. Journal of Experimental Bontany, v. 14, ed. 10, p. 1215-1221, 1990. COOKSON, S. J.; WILLIAMS, L. E.; MILLER, A. J. Light-dark changes in cytosolic nitrate pools depend on nitrate reductase activity in Arabidopsis leaf cells. Plant Physiology, v. 138, n. 2, p. 1097-1105, 2005. COOPER, G. M. The Cell: A Molecular Approach (2? ed.). Cap. 10:.The Chloroplast Genome". ASM Press. 2000. CRAWFORD, N. M. Nitrate: nutrient and signal for plant growth. The plant cell, v. 7, n. 7, p. 859, 1995. DELLEDONNE M, ZEIER J, MAROCCO A, LAMB C. Signal interactions between nitric oxide and reactive oxygen intermediates in the plant hypersensitive disease resistance response. Proceeding of the National Academy of Sciences, USA 98, 13454?13459, 2001 DE NISI, P., G. ZOCCHI. "Phosphoenolpyruvate carboxylase in cucumber (Cucumissativus L.) roots under iron deficiency: activity and kinetic characterization." Journal of Experimental Botany 51.352 : 1903-1909, 2000. DESIKAN R, GRIFFITHS R, HANCOCK J, NEILL S.A new role for an old enzyme: nitrate reductase-mediated nitric oxide generation is required for abscisic acid-induced stomatal closure in Arabidopsis thaliana.Proceedings of the National Academy of Sciences, v. 99, n. 25, p. 16314-16318, 2002. EDWARDS G.E., FRANCESCHI V.R., VOZNESENSKAYA E.V. ?Single-Cell C4 Photosynthesis Versus The Dual-Cell (Kranz) Paradigm?, Annual Review of Plant Biology, v. 55, 173-196, 2004 FARNDEN, K. J. S.; ROBERTSON, J. G. Methods for studying enzyme involved in metabolism related to nitrogen. In: BERGSEN, F. J. Ed. Methods for Evaluating Biological Nitrogen Fixation, 1980. FELKER, P. Micro determination of nitrogen in seed protein extracts. Analitical Chemical, v.49, p.1980, 1977. FERNANDES, M. S. N carriers, light and temperature influences on the free amino acid pool composition of rice plants. Turrialba, v.33, n.3, p.297-301, 1984. FERNANDES, M. S., ROSSIELLO, R. O. P. Mineral Nitrogen in Plant Physiology and Plant Nutrition, Critical Reviews in Plant Sciences, 14:2, 111-148, 1995. GARCIA-MATA, C., GAY, R., SOKOLOVSKI, S., HILLS, A., LAMATTINA, L., BLATT, M. R. Nitric oxide regulates K+ and Cl- channels in guard cells through a subset of abscisic acid-evoked signaling pathways. Proceedings of the National Academy of Sciences of the United States of America, v. 100, n. 19, p. 11116?11121, 2003. GONZ?LEZ M.C.,OSUNA L.,ECHEVARR?A C.,VIDAL J.,CEJUDO F.J. "Expression and localization of phosphoenolpyruvate carboxylase in developing and germinating wheat grains." Plant Physiology 116.4: 1249-1258, 1998. FITZGERALD, M. A.; MCCOUCH, S. R.; HALL, R. D. Not just a grain of rice: the quest for quality. Trends in Plant Science, v. 14, n. 3, p. 133?139, 2009. GE, Z.; RUBIO, G; LYNCH, J. P. The importance of root gravitropism for inter-root competition and phosphorous acquisition efficiency: Results from a geometric simulation model. Plant and Soil, v. 218, p. 159-171, 2000. GOOD, A. G.; SHRAWAT, A. K.; MUENCH, D. G. Can less yield more? Is reducing nutrient input into the environment compatible with maintaining crop production? Trends in Plant Science, v.9, n.12, p.597-605, 2004 GUALBERTI G, PAPI M, BELLUCCI L, RICCI I, BOUCHEZ D, CAMILLERI C, COSTANTINO P, VITTORIOSO P. Mutations in the Dof zinc finger genes DAG2 and DAG1 influence with opposite effects the germination of Arabidopsis seeds. Plant Cell, n. 6, p. 1253-1263, 2002. GUPTA, S., GUPTA, S. M., GUPTA, A. K., GAUR, V. S., KUMAR, A. Fluctuation of Dof1/Dof2 expression ratio under the influence of varying nitrogen and light conditions: 27 involvement in differential regulation of nitrogen metabolism in two genotypes of finger millet (Eleusine coracana L.). Gene, v. 546, n. 2, p. 327?335, 2014. HARPER, C. J., HAYWARD, D., KIDD, M., WIID, I., HELDEN, P. Glutamate dehydrogenase and glutamine synthetase are regulated in response to nitrogen availability in Myobacterium smegmatis. BMC microbiology, v. 10, p. 138, 2010. HIREL, B., GADAL, P. Glutamine Synthetase in Rice. Plant physiology, v. 66, n. 4, p. 619?623, 1980. HIREL, B., VIDAL, J., GADAL, P. Evidence for a cytosolic-dependent light induction of chloroplastic glutamine synthetase during greening of etiolated rice leaves. Planta, v. 155, t?pico 1, p. 17-23 (1982). HOAGLAND, D. R.; ARNON, D. I. The water-culture method for growing plants without soil. California Agricultural of Experimental Station Bull, v.347, p.1-32, 1950. HODGES, M., FLESCH, V., G?LVEZ, S., BISMUTH, E. Higher plant NADP+-dependent isocitrate dehydrogenases, ammonium assimilation and NADPH production. Plant Physiology and Biochemistry, v. 41, n. 6-7, p. 577?585, 2003. HOLSBEEKS I, LAGATIE O, VAN NULAND A, VAN DE VELDE S, THEVELEIN JM. The eukaryotic plasma membrane as a nutrient-sensing device. Trends in Biochemistry Science,v. 29:556?64, 2004. KANG, H. G., FOLEY, R.C., ONATE-SANCHEZ, L., LIN, C., SINGH, K.B. Target genes for OBP3, a Dof transcription factor, include novel basic helix?loop?helix domain proteins. Plant Journal,n. 35 p. 362?372, 2003. KROUK, G., TILLARD, P., GOJON, A. Regulation of the high-affinity NO3? uptake system by NRT1. 1-mediated NO3? demand signaling in Arabidopsis. Plant Physiology, v. 142, n. 3, p. 1075-1086, 2006. KURAI, T., WAKAYAMA, M., ABIKO, T., YANAGISAWA, S., AOKI, N., OHSUGI, R. Introduction of the ZmDof1 gene into rice enhances carbon and nitrogen assimilation under low?nitrogen conditions. Plant biotechnology journal, 9(8), 826-837, 2011. LEA, P. J., AZEVEDO, R. A. NITROGEN USE EFFICIENCY. 1. Uptake of nitrogen from the soil. Annals of Applied Biology, 149(3), 243-247, 2006. LEA, P. J., IRELAND, R., "Nitrogen metabolism in higher plants." Plant amino acids: Biochemistry and biotechnology, 1-47, 1999. LIJAVETZKY, D.; CARBONERO, P.; VICENTE-CARBAJOSA, J. Genome-wide comparative phylogenetic analysis of the rice and Arabidopsis Dof gene families. BMC evolutionary biology, v. 3, n. July 2015, p. 17, 2003. MASCLAUX-DAUBRESSE, C., REISDORF-CREN, M., PAGEAU, K., LELANDAIS, M., GRANDJEAN, O., KRONENBERGER, J., VALADIER, M., FERAUD, M., JOUGLET, T., SUZUKI, A. Glutamine synthetase-glutamate synthase pathway and glutamate dehydrogenase play distinct roles in the sink-source nitrogen cycle in tobacco. Plant Physiology, v. 140, n. 2, p. 444-456, 2006. MIFLIN, B. J., LEA, P.J. Amino acid metabolism. Ann. Rev. Plant Physiology, n. 28 p. 299-329, 1977. MIGGE A., CARRAYOL E., HIREL B.,BECKER T.W.Leaf-specific overexpression of plastidic glutamine synthetase stimulates the growth of transgenic tobacco seedlings. Planta, v. 210, n. 2, p. 252-260, 2000. MILLER, A. J., & CRAMER, M. D., Root nitrogen acquisition and assimilation. In Root Physiology: from Gene to Function, Springer Netherlands p. 1-36, 2005. MIRANDA, K. M.; ESPEY, M. G.; WINK, D. A. A rapid, simple spectrophotometric method for simultaneous detection of nitrate and nitrite. Nitric oxide : biology and chemistry / official journal of the Nitric Oxide Society, v. 5, n. 1, p. 62?71, 2001. MORENO-RISUENOM. A.,MART?NEZM.,VICENTE-CARBAJOSAJ., CARBONERO P. The family of DOF transcription factors: From green unicellular algae to vascular plants. Molecular Genetics and Genomics, v. 277, n. 4, p. 379?390, 2007. NIELSEN, K. L.; MILLER, C. R.; BECK, D.; LYNCH, J. P. Fractal geometry of root system: Field observation of contrasting genotype of common bean (Phaseolus vulgaris L.) grown under different phosphorous regimes. Plant and Soil, v. 206, p.181-190, 1999. NOGUERO M., ATIF R.M., OCHATT S., THOMPSON R.D. "The role of the DNA-binding One Zinc Finger (DOF) transcription factor family in plants, Plant Science, v.209, 32-45, 2013. PAPAVASSILIOU A. G., Transcription Factor-Based Drug Design in Anticancer Drug Development. Molecular Medicine, v.3, n.12, p.799-810, 1997 PEOPLES, M. B.; HERRIDGE, D. F.; LADHA, J. K. Biological nitrogen fixation: An efficient source of nitrogen for sustainable agricultural production? Plant Soil, v.174, p.3?28, 1995. P?REZ-RODRIGUES, P., RIA?O-PANCHON, D. M., CORR?A, L. G. G., RENSING, S. A., KERSTEN, B., MUELLER-ROEBER, B. PlnTFDB: updated content and new features of the plant transcription factor database. Nucleic Acid Research, n. 10, p. 1-6, 2009. PLESCH, G., EHRHARDT, T.,MUELLER-ROEBER, B. Involvement of TAAAG elements suggests a role for Dof transcription factors in guard cell- specific gene expression. Plant Jn. 28, p.455?464, 2001. ROBERTSON, G. P., VITOUSEK, P. M. Nitrogen in Agriculture: Balancing the Cost of an Essential Resource. Annual Review of Environmental Resourses. n. 34, p. 97-125, 2009. RUEDA-L?PEZ, M., GARC?A-GUTI?RREZ, A., C?NOVAS, F. M., ?VILA, C. The family of Dof transcription factors in pine. Trees - Structure and Function, v. 27, p. 1547?1557, 2013. SANTOS, L. A.; DE SOUZA, S. R.; FERNANDES, M. S. OsDof25 expression alters carbon and nitrogen metabolism in Arabidopsis under high N-supply. Plant Biotechnology Reports, v. 6, p. 327?337, 2012. SCHIAVON, M., ERTANI, A., NARDI, S. "Effects of an alfalfa protein hydrolysate on the gene expression and activity of enzymes of the tricarboxylic acid (TCA) cycle and nitrogen metabolism in Zea mays L." Journal of agricultural and food chemistry 56.24: 11800-11808, 2008. SETI?N, I., VEGA-MAS, I., CELESTINO, N., CALLEJA-CERVANTES, M. E., GONZ?LEZ-MURUA, C., ESTAVILLO, J. M., GONZ?LEZ-MORO, M. B. Root phosphoenolpyruvate carboxylase and NAD-malic enzymes activity increase the ammoniumassimilating capacity in tomato.Journal of plant physiology, v. 171, n. 5, p. 49-63, 2014. SILVA, R.A.C.Efeito da superexpress?o do Fator de Transcri??o OsDof25 sobre a Efici?ncia de Absor??o de Nitrog?nio em Oryza sativa L.2012. 68f. (Disserta??o de Mestrado em Agronomia ? Ci?ncia do Solo) Serop?dica- RJ; UFRRJ. SHIGYO, M., TABEI, N., YONEYAMA, T., YANAGISAWA S., Evolutionary processes during the formation of the plant-specific Dof transcription factor family. Plant Cell Physyiology, n. 48, p. 179-185, 2007. SHIMOFURUTANI, N., KISU, Y., SUZUKI, M, ESAKA, M. Functional analyses of the Dof domain, a zinc finger DNA-binding domain, in a pumpkin DNA-binding protein AOBP. FEBS Letters, v. 430, n 3, p. 251-256, 1998. SKIRYCZ, A., REICHELT, M., BUROW, M., BIRKEMEYER, C., ROLCIK, J.; DOF transcription factor AtDOF1.1 (OBP2) is part of a regulatory net- work controlling glucosinolate biosynthesis in Arabidopsis. Plant Journal; n. 47, p. 10?24, 2006. SOUZA, S. R., STARK, E. M. L. M., FERNANDES, M. S., MAGALH?ES, J. R., Effect of supplemental nitrogen on nitrogen-assimilation enzymes, free amino nitrogen, soluble sugars, and crude protein of rice. Communications in Soil Science and Plant Analysis, 30:5-6, 711-724, 1999. SOUZA, S. R., FERNANDES, M. S., Nutri??o Mineral de Plantas, Ed. UFV, cap?tulo 9, p. 216-251, 2006. SOUZA, V.M. Caracteriza??o Funcional do OsDof25 e Seus Efeitos Sobre o Metabolismo de Nitrog?nio e Carbono em Arroz. 2014. 135f. (Tese de doutoradoemFitotecnia) Serop?dica-RJ; UFRRJ. SPERANDIO, M. V. L., SANTOS, L. A., ARA?JO, O. J. L., BRAGA, R. P., COELHO, P. C., NOGUEIRA, E. M., FERNANDES, M. S., SOUZA, S. R., Response of nitrate transporters and PM H+-ATPase expression to nitrogen flush on two upland rice varieties contrasting in nitrate uptake kinetics. Australian Journal of Crop Science, v. 8, n. 4, p. 568-576, 2014. SUGIYAMA, T., ISHIDA, T., TABEI, N., SHIGYO, M., KONISHI, M., YONEYAMA, T., YANAGISAWA, S..Involvement of PpDof1 transcriptional repressor in the nutrient condition-dependent growth control of protonemal filaments in Physcomitrella patens.Journal of Experimental Botany, v. 63, n. 8, p. 3185?3197, 2012. SUZUKI, A., VIDAL, J., GADAL, P., Glutamate synthase isoforms in rice. Immunological studies on enzymes in green leaf, etiolated leaf and root tissues. PlantPhysiology, n. 70, p. 827-832, 1982. TAIZ, L.; ZEIGER, E. Plant physiology, Sunderland: Sinauer Associates, 2003. 690p. TAVARES, O. C. H. Efeito dos ?cidos H?micos Sobre as H+-ATPase, Transportadores de N-NO3 - e N- NH4+, e Sobre o Crescimento em Arroz. 2014.100f. Tese (Doutorado em ci?ncias). Instituto de Agronomia, Departamento de Fitotecnia, Universidade Federal Rural do Rio de Janeiro, Serop?dica, RJ, 2014. TURANO F.J.,DASHNER R.,UPADHYAYA A.,CALDWELL C.R."Purification of mitochondrial glutamate dehydrogenase from dark-grown soybean seedlings" Plant Physiology 112.3 (1996): 1357-1364. WANG, Y., FU, B., PAN, L., CHEN, L., FU, X., LI, K. Overexpression of Arabidopsis Dof1, GS1 and GS2 enhanced nitrogen assimilation in transgenic tobacco grown under low-nitrogen conditions. Plant Molecular Biology Reporter, v. 31, n. 4, p. 886-900, 2013. WARD, J. M., CUFR, C. A., DENZEL, M. A., NEFF, M. M. The Dof transcription factor OBP3 modulates phytochrome and cryptochrome signaling in Arabidopsis. Plant Cell, v. 17, n. 2, p. 475?485, 2005. WEI, P.C., TAN, F., GAO, X.Q., ZHANG, X.Q., WANG, G.Q., XU, H., LI, L.J., CHEN, J.,WANG, X.C.; Overexpression of AtDOF4.7, an Arabidopsis DOF family transcription factor, induces floral organ abscission deficiency in Arabidopsis. Plant Physiol n. 153, p. 1031?1045, 2010. XU, G.; FAN, X.; MILLER, A. J. Plant Nitrogen Assimilation and Use Efficiency. Annual Review of Plant Biology, v. 63, n. December, p. 153?182, 2012. YAMASAKI, H., SAKIHAMA, Y.. Simultaneous production of nitric oxide and peroxynitrite by plant nitrate reductase: in vitro evidence for the NR-dependent formation of active nitrogen species.FEBS Letters, n. 468, p.89-92, 2000. YANAGISAWA, S.; SHEEN, J. Involvement of maize Dof zinc finger proteins in tissuespecific and light-regulated gene expression. The Plant cell, v. 10, n. 1, p. 75?89, 1998. YANAGISAWA, S. Dof1 and DOF2 transcription factors are associated with expression of multiple genes involved in carbon metabolism in maize. The Plant Journal, 21(3), 281-288, 2000. YANAGISAWA, S. The DOF family of plant transcription factors. TRENDS in Plant Science, v.7 n.12, p.555-560, 2002. YANAGISAWA, S.; AKIYAMA, A.; KISAKA, H.; UCHIMIYA, H.; MIWA, T. Metabolic engineering with Dof1 transcription factor in plants: Improved nitrogen assimilation and growth under low-nitrogen conditions. Proceedings of the National Academy of Sciences, v.101, n.20, p.7833-7838, 2004a. YANAGISAWA, S. DOF Domain Proteins: Plant-Specific Transcription Factors Associated with Diverse Phenomena Unique to Plants. Plant Cell Physiol., v.45, n.4, p.386-391, 2004b. YANAGISAWA, S. Plant Science Transcription factors involved in controlling the expression of nitrate reductase genes in higher plants. Plant Science, v. 229, p. 167?171, 2014. YANAGISAWA, S., Structure, Function, and Evolutionof the Dof Transcription Factor Family. Plant Transcription Factors ? Evolutionary, Structural and Function Aspects. Academic Press, p.183-197. YANG, X., TUSKAN, G. A., CHENG, Z. -M. Divergence of the Dof gene families in poplar, Arabidopsis, and Rice suggests multiple modes of gene evolution after duplication. Plant Physiology, n. 142, p. 820-830, 2006. YEMM, E.W. & COCKING, E. C. The determination of amino-acid with ninhydrin. Analytical Biochemistry, v.80, p.209-213, 1955. YEMM, E.W. & WILLIS, A. J. The estimation of carbohydrate in plants extracts by anthrone. Biochemistry, v.57, p.508-514, 1954. ZHANG, Y., VERHOEFF, N., CHEN, Z., CHEN, S., WANG, M., ZHU, Z., OUWEKERK, P. B. Functions of OsDof25 in regulation of OsC4PPDK. Plant Molecular Biology, 2015. ZHAO, P., SUN, G.C., PENG, S.L., Ecophysiological research on nitrogen nutrition of plants. Ecol. 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| dc.title.por.fl_str_mv |
Avalia??o do efeito da superexpress?o do fator de transcri??o OsDof25 sobre o metabolismo e desenvolvimento radicular em arroz |
| dc.title.alternative.eng.fl_str_mv |
Effect of overexpression of transcription factor OsDof25 over the metabolism and root development in rice |
| title |
Avalia??o do efeito da superexpress?o do fator de transcri??o OsDof25 sobre o metabolismo e desenvolvimento radicular em arroz |
| spellingShingle |
Avalia??o do efeito da superexpress?o do fator de transcri??o OsDof25 sobre o metabolismo e desenvolvimento radicular em arroz Braga, Renan Pinto Nitrog?nio Glutamina Raiz Nitrogen Glutamine Root Agronomia |
| title_short |
Avalia??o do efeito da superexpress?o do fator de transcri??o OsDof25 sobre o metabolismo e desenvolvimento radicular em arroz |
| title_full |
Avalia??o do efeito da superexpress?o do fator de transcri??o OsDof25 sobre o metabolismo e desenvolvimento radicular em arroz |
| title_fullStr |
Avalia??o do efeito da superexpress?o do fator de transcri??o OsDof25 sobre o metabolismo e desenvolvimento radicular em arroz |
| title_full_unstemmed |
Avalia??o do efeito da superexpress?o do fator de transcri??o OsDof25 sobre o metabolismo e desenvolvimento radicular em arroz |
| title_sort |
Avalia??o do efeito da superexpress?o do fator de transcri??o OsDof25 sobre o metabolismo e desenvolvimento radicular em arroz |
| author |
Braga, Renan Pinto |
| author_facet |
Braga, Renan Pinto |
| author_role |
author |
| dc.contributor.advisor1.fl_str_mv |
Fernandes, Manlio Silvestre |
| dc.contributor.advisor1ID.fl_str_mv |
002.180.573-34 |
| dc.contributor.advisor-co1.fl_str_mv |
Souza, Sonia Regina de |
| dc.contributor.advisor-co1ID.fl_str_mv |
003-927.487-03 |
| dc.contributor.referee1.fl_str_mv |
Ferraz Junior, Altamiro Souza de Lima |
| dc.contributor.referee2.fl_str_mv |
Garrido, Rodrigo Grazinoli |
| dc.contributor.authorID.fl_str_mv |
059.359.317-02 |
| dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/5205853825951525 |
| dc.contributor.author.fl_str_mv |
Braga, Renan Pinto |
| contributor_str_mv |
Fernandes, Manlio Silvestre Souza, Sonia Regina de Ferraz Junior, Altamiro Souza de Lima Garrido, Rodrigo Grazinoli |
| dc.subject.por.fl_str_mv |
Nitrog?nio Glutamina Raiz |
| topic |
Nitrog?nio Glutamina Raiz Nitrogen Glutamine Root Agronomia |
| dc.subject.eng.fl_str_mv |
Nitrogen Glutamine Root |
| dc.subject.cnpq.fl_str_mv |
Agronomia |
| description |
One of the main targets in plants of agricultural interest is the Nitrogen Use Efficiency (NUE). The study of regulatory proteins related to gene expression, specifically transcription factors, is a viable alternative for controlling quantitative traits such as NUE. The transcription factor OsDof25 is an ortholog ZmDof1 maize gene identified already controlling the metabolism of carbon (C). This study was conducted to evaluate N metabolism and C in rice lines overexpressing the transcription factor OsDof25 and morphophysiological changes generated by overexpression, aiming characteristics that lead to a better NUE. Lines showed increased activity in of nitrogen metabolism enzymes (Nitrate Reductase, Glutamine synthetase and glutamate synthase) and higher content of primary metabolites, indicating better assimilation efficiency. The lines L#9.6 and L#10.8 showed high development of roots, compared to wild type. The transformed lines also showed greater number of very fine roots, as well as increased volume, surface area and length, important features for the acquisition and search for nutrients and water. |
| publishDate |
2016 |
| dc.date.issued.fl_str_mv |
2016-02-26 |
| dc.date.accessioned.fl_str_mv |
2021-12-28T00:33:28Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
| format |
masterThesis |
| status_str |
publishedVersion |
| dc.identifier.citation.fl_str_mv |
BRAGA, Renan Pinto. Avalia??o do efeito da superexpress?o do fator de transcri??o OsDof25 sobre o metabolismo e desenvolvimento radicular em arroz. 2016. 34 f. Disserta??o (Mestrado em Agronomia, Ci?ncia do Solo) - Instituto de Agronomia, Universidade Federal Rural do Rio de Janeiro, Serop?dica-RJ, 2016. |
| dc.identifier.uri.fl_str_mv |
https://tede.ufrrj.br/jspui/handle/jspui/5296 |
| identifier_str_mv |
BRAGA, Renan Pinto. Avalia??o do efeito da superexpress?o do fator de transcri??o OsDof25 sobre o metabolismo e desenvolvimento radicular em arroz. 2016. 34 f. Disserta??o (Mestrado em Agronomia, Ci?ncia do Solo) - Instituto de Agronomia, Universidade Federal Rural do Rio de Janeiro, Serop?dica-RJ, 2016. |
| url |
https://tede.ufrrj.br/jspui/handle/jspui/5296 |
| dc.language.iso.fl_str_mv |
por |
| language |
por |
| dc.relation.references.por.fl_str_mv |
BAUMANN, K., GUALBERTI, G. The DNA-binding site of the Dof protein NtBBF1 is essential for tissue-specific and auxin-regulated expression of the rolB oncogene in plants. Plant Cell n.11, p.323-333, 1999. BARBER, S. A. Soil nutrient bioavailability: a mechanistic approach. John Wiley & Sons, 1995. BERNARD, S. M., M?LLER, A.L., DIONISIO, G., KICHEY, T., JAHN, T.P., DUBOIS, F., BAUDO, M., LOPES, M.S., TERC?-LAFORGUE, T., FOYER, C.H., PARRY, M.A., FORDE, B.G., ARAUS, J.L., HIREL, B., SCHJOERRING, J.K., HABASH, D.Z. Gene expression, cellular localisation and function of glutamine synthetase isozymes in wheat (Triticum aestivum L.). Plant Molecular Biology, v. 67, n. 1-2, p. 89-105, 2008. BRADFORD, M.M. Rapid and sensitive method for quantification of microgram quantities of protein utilizing the principle of protein dye-binding. Analytical Biochemistry, v.72, p.248-254, 1976. CAI, H., ZHOU, Y., XIAO, J., LI, X., ZHANG, Q., LIAN, X., Overexpressed glutamine synthetase gene modifies nitrogen metabolism and abiotic stress responses in rice. Plant Cell Reports, v. 28, n. 3, p. 527-537, 2009. CHASTAIN, C. J., FAILING, C. J., MANANDHAR, L., ZIMMERMAN, M. A., LAKNER, M. M., NGUYEN, TONY. H. Functional evolution of C4 pyruvate, orthophosphate dikinase. Journal of Experimental Botany, v. 62, n. 9, p. 3083?3091, 2011. CHEN, F. L., BENNETT, M. J., CULLIMORE, J. V. Effect of the Nitrogen Supply on the Activities of Isoenzymes of NADH-dependent Glutamate Synthase and Glutamine Synthetase in Root Nodules of Phaseolus vulgaris L. Journal of Experimental Bontany, v. 14, ed. 10, p. 1215-1221, 1990. COOKSON, S. J.; WILLIAMS, L. E.; MILLER, A. J. Light-dark changes in cytosolic nitrate pools depend on nitrate reductase activity in Arabidopsis leaf cells. Plant Physiology, v. 138, n. 2, p. 1097-1105, 2005. COOPER, G. M. The Cell: A Molecular Approach (2? ed.). Cap. 10:.The Chloroplast Genome". ASM Press. 2000. CRAWFORD, N. M. Nitrate: nutrient and signal for plant growth. The plant cell, v. 7, n. 7, p. 859, 1995. DELLEDONNE M, ZEIER J, MAROCCO A, LAMB C. Signal interactions between nitric oxide and reactive oxygen intermediates in the plant hypersensitive disease resistance response. Proceeding of the National Academy of Sciences, USA 98, 13454?13459, 2001 DE NISI, P., G. ZOCCHI. "Phosphoenolpyruvate carboxylase in cucumber (Cucumissativus L.) roots under iron deficiency: activity and kinetic characterization." Journal of Experimental Botany 51.352 : 1903-1909, 2000. DESIKAN R, GRIFFITHS R, HANCOCK J, NEILL S.A new role for an old enzyme: nitrate reductase-mediated nitric oxide generation is required for abscisic acid-induced stomatal closure in Arabidopsis thaliana.Proceedings of the National Academy of Sciences, v. 99, n. 25, p. 16314-16318, 2002. EDWARDS G.E., FRANCESCHI V.R., VOZNESENSKAYA E.V. ?Single-Cell C4 Photosynthesis Versus The Dual-Cell (Kranz) Paradigm?, Annual Review of Plant Biology, v. 55, 173-196, 2004 FARNDEN, K. J. S.; ROBERTSON, J. G. Methods for studying enzyme involved in metabolism related to nitrogen. In: BERGSEN, F. J. Ed. Methods for Evaluating Biological Nitrogen Fixation, 1980. FELKER, P. Micro determination of nitrogen in seed protein extracts. Analitical Chemical, v.49, p.1980, 1977. FERNANDES, M. S. N carriers, light and temperature influences on the free amino acid pool composition of rice plants. Turrialba, v.33, n.3, p.297-301, 1984. FERNANDES, M. S., ROSSIELLO, R. O. P. Mineral Nitrogen in Plant Physiology and Plant Nutrition, Critical Reviews in Plant Sciences, 14:2, 111-148, 1995. GARCIA-MATA, C., GAY, R., SOKOLOVSKI, S., HILLS, A., LAMATTINA, L., BLATT, M. R. Nitric oxide regulates K+ and Cl- channels in guard cells through a subset of abscisic acid-evoked signaling pathways. Proceedings of the National Academy of Sciences of the United States of America, v. 100, n. 19, p. 11116?11121, 2003. GONZ?LEZ M.C.,OSUNA L.,ECHEVARR?A C.,VIDAL J.,CEJUDO F.J. "Expression and localization of phosphoenolpyruvate carboxylase in developing and germinating wheat grains." Plant Physiology 116.4: 1249-1258, 1998. FITZGERALD, M. A.; MCCOUCH, S. R.; HALL, R. D. Not just a grain of rice: the quest for quality. Trends in Plant Science, v. 14, n. 3, p. 133?139, 2009. GE, Z.; RUBIO, G; LYNCH, J. P. The importance of root gravitropism for inter-root competition and phosphorous acquisition efficiency: Results from a geometric simulation model. Plant and Soil, v. 218, p. 159-171, 2000. GOOD, A. G.; SHRAWAT, A. K.; MUENCH, D. G. Can less yield more? Is reducing nutrient input into the environment compatible with maintaining crop production? Trends in Plant Science, v.9, n.12, p.597-605, 2004 GUALBERTI G, PAPI M, BELLUCCI L, RICCI I, BOUCHEZ D, CAMILLERI C, COSTANTINO P, VITTORIOSO P. Mutations in the Dof zinc finger genes DAG2 and DAG1 influence with opposite effects the germination of Arabidopsis seeds. Plant Cell, n. 6, p. 1253-1263, 2002. GUPTA, S., GUPTA, S. M., GUPTA, A. K., GAUR, V. S., KUMAR, A. Fluctuation of Dof1/Dof2 expression ratio under the influence of varying nitrogen and light conditions: 27 involvement in differential regulation of nitrogen metabolism in two genotypes of finger millet (Eleusine coracana L.). Gene, v. 546, n. 2, p. 327?335, 2014. HARPER, C. J., HAYWARD, D., KIDD, M., WIID, I., HELDEN, P. Glutamate dehydrogenase and glutamine synthetase are regulated in response to nitrogen availability in Myobacterium smegmatis. BMC microbiology, v. 10, p. 138, 2010. HIREL, B., GADAL, P. Glutamine Synthetase in Rice. Plant physiology, v. 66, n. 4, p. 619?623, 1980. HIREL, B., VIDAL, J., GADAL, P. Evidence for a cytosolic-dependent light induction of chloroplastic glutamine synthetase during greening of etiolated rice leaves. Planta, v. 155, t?pico 1, p. 17-23 (1982). HOAGLAND, D. R.; ARNON, D. I. The water-culture method for growing plants without soil. California Agricultural of Experimental Station Bull, v.347, p.1-32, 1950. HODGES, M., FLESCH, V., G?LVEZ, S., BISMUTH, E. Higher plant NADP+-dependent isocitrate dehydrogenases, ammonium assimilation and NADPH production. Plant Physiology and Biochemistry, v. 41, n. 6-7, p. 577?585, 2003. HOLSBEEKS I, LAGATIE O, VAN NULAND A, VAN DE VELDE S, THEVELEIN JM. The eukaryotic plasma membrane as a nutrient-sensing device. Trends in Biochemistry Science,v. 29:556?64, 2004. KANG, H. G., FOLEY, R.C., ONATE-SANCHEZ, L., LIN, C., SINGH, K.B. Target genes for OBP3, a Dof transcription factor, include novel basic helix?loop?helix domain proteins. Plant Journal,n. 35 p. 362?372, 2003. KROUK, G., TILLARD, P., GOJON, A. Regulation of the high-affinity NO3? uptake system by NRT1. 1-mediated NO3? demand signaling in Arabidopsis. Plant Physiology, v. 142, n. 3, p. 1075-1086, 2006. KURAI, T., WAKAYAMA, M., ABIKO, T., YANAGISAWA, S., AOKI, N., OHSUGI, R. Introduction of the ZmDof1 gene into rice enhances carbon and nitrogen assimilation under low?nitrogen conditions. Plant biotechnology journal, 9(8), 826-837, 2011. LEA, P. J., AZEVEDO, R. A. NITROGEN USE EFFICIENCY. 1. Uptake of nitrogen from the soil. Annals of Applied Biology, 149(3), 243-247, 2006. LEA, P. J., IRELAND, R., "Nitrogen metabolism in higher plants." Plant amino acids: Biochemistry and biotechnology, 1-47, 1999. LIJAVETZKY, D.; CARBONERO, P.; VICENTE-CARBAJOSA, J. Genome-wide comparative phylogenetic analysis of the rice and Arabidopsis Dof gene families. BMC evolutionary biology, v. 3, n. July 2015, p. 17, 2003. MASCLAUX-DAUBRESSE, C., REISDORF-CREN, M., PAGEAU, K., LELANDAIS, M., GRANDJEAN, O., KRONENBERGER, J., VALADIER, M., FERAUD, M., JOUGLET, T., SUZUKI, A. Glutamine synthetase-glutamate synthase pathway and glutamate dehydrogenase play distinct roles in the sink-source nitrogen cycle in tobacco. Plant Physiology, v. 140, n. 2, p. 444-456, 2006. MIFLIN, B. J., LEA, P.J. Amino acid metabolism. Ann. Rev. Plant Physiology, n. 28 p. 299-329, 1977. MIGGE A., CARRAYOL E., HIREL B.,BECKER T.W.Leaf-specific overexpression of plastidic glutamine synthetase stimulates the growth of transgenic tobacco seedlings. Planta, v. 210, n. 2, p. 252-260, 2000. MILLER, A. J., & CRAMER, M. D., Root nitrogen acquisition and assimilation. In Root Physiology: from Gene to Function, Springer Netherlands p. 1-36, 2005. MIRANDA, K. M.; ESPEY, M. G.; WINK, D. A. A rapid, simple spectrophotometric method for simultaneous detection of nitrate and nitrite. Nitric oxide : biology and chemistry / official journal of the Nitric Oxide Society, v. 5, n. 1, p. 62?71, 2001. MORENO-RISUENOM. A.,MART?NEZM.,VICENTE-CARBAJOSAJ., CARBONERO P. The family of DOF transcription factors: From green unicellular algae to vascular plants. Molecular Genetics and Genomics, v. 277, n. 4, p. 379?390, 2007. NIELSEN, K. L.; MILLER, C. R.; BECK, D.; LYNCH, J. P. Fractal geometry of root system: Field observation of contrasting genotype of common bean (Phaseolus vulgaris L.) grown under different phosphorous regimes. Plant and Soil, v. 206, p.181-190, 1999. NOGUERO M., ATIF R.M., OCHATT S., THOMPSON R.D. "The role of the DNA-binding One Zinc Finger (DOF) transcription factor family in plants, Plant Science, v.209, 32-45, 2013. PAPAVASSILIOU A. G., Transcription Factor-Based Drug Design in Anticancer Drug Development. Molecular Medicine, v.3, n.12, p.799-810, 1997 PEOPLES, M. B.; HERRIDGE, D. F.; LADHA, J. K. Biological nitrogen fixation: An efficient source of nitrogen for sustainable agricultural production? Plant Soil, v.174, p.3?28, 1995. P?REZ-RODRIGUES, P., RIA?O-PANCHON, D. M., CORR?A, L. G. G., RENSING, S. A., KERSTEN, B., MUELLER-ROEBER, B. PlnTFDB: updated content and new features of the plant transcription factor database. Nucleic Acid Research, n. 10, p. 1-6, 2009. PLESCH, G., EHRHARDT, T.,MUELLER-ROEBER, B. Involvement of TAAAG elements suggests a role for Dof transcription factors in guard cell- specific gene expression. Plant Jn. 28, p.455?464, 2001. ROBERTSON, G. P., VITOUSEK, P. M. Nitrogen in Agriculture: Balancing the Cost of an Essential Resource. Annual Review of Environmental Resourses. n. 34, p. 97-125, 2009. RUEDA-L?PEZ, M., GARC?A-GUTI?RREZ, A., C?NOVAS, F. M., ?VILA, C. The family of Dof transcription factors in pine. Trees - Structure and Function, v. 27, p. 1547?1557, 2013. SANTOS, L. A.; DE SOUZA, S. R.; FERNANDES, M. S. OsDof25 expression alters carbon and nitrogen metabolism in Arabidopsis under high N-supply. Plant Biotechnology Reports, v. 6, p. 327?337, 2012. SCHIAVON, M., ERTANI, A., NARDI, S. "Effects of an alfalfa protein hydrolysate on the gene expression and activity of enzymes of the tricarboxylic acid (TCA) cycle and nitrogen metabolism in Zea mays L." Journal of agricultural and food chemistry 56.24: 11800-11808, 2008. SETI?N, I., VEGA-MAS, I., CELESTINO, N., CALLEJA-CERVANTES, M. E., GONZ?LEZ-MURUA, C., ESTAVILLO, J. M., GONZ?LEZ-MORO, M. B. Root phosphoenolpyruvate carboxylase and NAD-malic enzymes activity increase the ammoniumassimilating capacity in tomato.Journal of plant physiology, v. 171, n. 5, p. 49-63, 2014. SILVA, R.A.C.Efeito da superexpress?o do Fator de Transcri??o OsDof25 sobre a Efici?ncia de Absor??o de Nitrog?nio em Oryza sativa L.2012. 68f. (Disserta??o de Mestrado em Agronomia ? Ci?ncia do Solo) Serop?dica- RJ; UFRRJ. SHIGYO, M., TABEI, N., YONEYAMA, T., YANAGISAWA S., Evolutionary processes during the formation of the plant-specific Dof transcription factor family. Plant Cell Physyiology, n. 48, p. 179-185, 2007. SHIMOFURUTANI, N., KISU, Y., SUZUKI, M, ESAKA, M. Functional analyses of the Dof domain, a zinc finger DNA-binding domain, in a pumpkin DNA-binding protein AOBP. FEBS Letters, v. 430, n 3, p. 251-256, 1998. SKIRYCZ, A., REICHELT, M., BUROW, M., BIRKEMEYER, C., ROLCIK, J.; DOF transcription factor AtDOF1.1 (OBP2) is part of a regulatory net- work controlling glucosinolate biosynthesis in Arabidopsis. Plant Journal; n. 47, p. 10?24, 2006. SOUZA, S. R., STARK, E. M. L. M., FERNANDES, M. S., MAGALH?ES, J. R., Effect of supplemental nitrogen on nitrogen-assimilation enzymes, free amino nitrogen, soluble sugars, and crude protein of rice. Communications in Soil Science and Plant Analysis, 30:5-6, 711-724, 1999. SOUZA, S. R., FERNANDES, M. S., Nutri??o Mineral de Plantas, Ed. UFV, cap?tulo 9, p. 216-251, 2006. SOUZA, V.M. Caracteriza??o Funcional do OsDof25 e Seus Efeitos Sobre o Metabolismo de Nitrog?nio e Carbono em Arroz. 2014. 135f. (Tese de doutoradoemFitotecnia) Serop?dica-RJ; UFRRJ. SPERANDIO, M. V. L., SANTOS, L. A., ARA?JO, O. J. L., BRAGA, R. P., COELHO, P. C., NOGUEIRA, E. M., FERNANDES, M. S., SOUZA, S. R., Response of nitrate transporters and PM H+-ATPase expression to nitrogen flush on two upland rice varieties contrasting in nitrate uptake kinetics. Australian Journal of Crop Science, v. 8, n. 4, p. 568-576, 2014. SUGIYAMA, T., ISHIDA, T., TABEI, N., SHIGYO, M., KONISHI, M., YONEYAMA, T., YANAGISAWA, S..Involvement of PpDof1 transcriptional repressor in the nutrient condition-dependent growth control of protonemal filaments in Physcomitrella patens.Journal of Experimental Botany, v. 63, n. 8, p. 3185?3197, 2012. SUZUKI, A., VIDAL, J., GADAL, P., Glutamate synthase isoforms in rice. Immunological studies on enzymes in green leaf, etiolated leaf and root tissues. PlantPhysiology, n. 70, p. 827-832, 1982. TAIZ, L.; ZEIGER, E. Plant physiology, Sunderland: Sinauer Associates, 2003. 690p. TAVARES, O. C. H. Efeito dos ?cidos H?micos Sobre as H+-ATPase, Transportadores de N-NO3 - e N- NH4+, e Sobre o Crescimento em Arroz. 2014.100f. Tese (Doutorado em ci?ncias). Instituto de Agronomia, Departamento de Fitotecnia, Universidade Federal Rural do Rio de Janeiro, Serop?dica, RJ, 2014. TURANO F.J.,DASHNER R.,UPADHYAYA A.,CALDWELL C.R."Purification of mitochondrial glutamate dehydrogenase from dark-grown soybean seedlings" Plant Physiology 112.3 (1996): 1357-1364. WANG, Y., FU, B., PAN, L., CHEN, L., FU, X., LI, K. Overexpression of Arabidopsis Dof1, GS1 and GS2 enhanced nitrogen assimilation in transgenic tobacco grown under low-nitrogen conditions. Plant Molecular Biology Reporter, v. 31, n. 4, p. 886-900, 2013. WARD, J. M., CUFR, C. A., DENZEL, M. A., NEFF, M. M. The Dof transcription factor OBP3 modulates phytochrome and cryptochrome signaling in Arabidopsis. Plant Cell, v. 17, n. 2, p. 475?485, 2005. WEI, P.C., TAN, F., GAO, X.Q., ZHANG, X.Q., WANG, G.Q., XU, H., LI, L.J., CHEN, J.,WANG, X.C.; Overexpression of AtDOF4.7, an Arabidopsis DOF family transcription factor, induces floral organ abscission deficiency in Arabidopsis. Plant Physiol n. 153, p. 1031?1045, 2010. XU, G.; FAN, X.; MILLER, A. J. Plant Nitrogen Assimilation and Use Efficiency. Annual Review of Plant Biology, v. 63, n. December, p. 153?182, 2012. YAMASAKI, H., SAKIHAMA, Y.. Simultaneous production of nitric oxide and peroxynitrite by plant nitrate reductase: in vitro evidence for the NR-dependent formation of active nitrogen species.FEBS Letters, n. 468, p.89-92, 2000. YANAGISAWA, S.; SHEEN, J. Involvement of maize Dof zinc finger proteins in tissuespecific and light-regulated gene expression. The Plant cell, v. 10, n. 1, p. 75?89, 1998. YANAGISAWA, S. Dof1 and DOF2 transcription factors are associated with expression of multiple genes involved in carbon metabolism in maize. The Plant Journal, 21(3), 281-288, 2000. YANAGISAWA, S. The DOF family of plant transcription factors. TRENDS in Plant Science, v.7 n.12, p.555-560, 2002. YANAGISAWA, S.; AKIYAMA, A.; KISAKA, H.; UCHIMIYA, H.; MIWA, T. Metabolic engineering with Dof1 transcription factor in plants: Improved nitrogen assimilation and growth under low-nitrogen conditions. Proceedings of the National Academy of Sciences, v.101, n.20, p.7833-7838, 2004a. YANAGISAWA, S. DOF Domain Proteins: Plant-Specific Transcription Factors Associated with Diverse Phenomena Unique to Plants. Plant Cell Physiol., v.45, n.4, p.386-391, 2004b. YANAGISAWA, S. Plant Science Transcription factors involved in controlling the expression of nitrate reductase genes in higher plants. Plant Science, v. 229, p. 167?171, 2014. YANAGISAWA, S., Structure, Function, and Evolutionof the Dof Transcription Factor Family. Plant Transcription Factors ? Evolutionary, Structural and Function Aspects. Academic Press, p.183-197. YANG, X., TUSKAN, G. A., CHENG, Z. -M. Divergence of the Dof gene families in poplar, Arabidopsis, and Rice suggests multiple modes of gene evolution after duplication. Plant Physiology, n. 142, p. 820-830, 2006. YEMM, E.W. & COCKING, E. C. The determination of amino-acid with ninhydrin. Analytical Biochemistry, v.80, p.209-213, 1955. YEMM, E.W. & WILLIS, A. J. The estimation of carbohydrate in plants extracts by anthrone. Biochemistry, v.57, p.508-514, 1954. ZHANG, Y., VERHOEFF, N., CHEN, Z., CHEN, S., WANG, M., ZHU, Z., OUWEKERK, P. B. Functions of OsDof25 in regulation of OsC4PPDK. Plant Molecular Biology, 2015. ZHAO, P., SUN, G.C., PENG, S.L., Ecophysiological research on nitrogen nutrition of plants. Ecol. Sci. 17, 36?42, 1998. |
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