C?lulas derivadas do tecido adiposo: uma possibilidade terap?utica para o infarto do mioc?rdio

• Autor(a) principal: Miranda, Amarildo
• Data de Publicação: 2015
• Tipo de documento: Tese
• Idioma: por
• Título da fonte: Biblioteca Digital de Teses e Dissertações da UFRRJ
• Texto Completo: https://tede.ufrrj.br/jspui/handle/jspui/3224
• topic: infarto do mioc?rdio; Tecido adiposo; Terapia celular; myocardial infarction; Adipose tissue; cell-based therapy; Fisiologia

Cardiovascular diseases are among the leading causes of death and myocardial infarction (IM) as the main one. Although pharmacological and interventional advances have reduced the morbidity and mortality of ischemic heart disease, there is an ongoing need for novel therapeutic strategies that prevent or reverse progressive ventricular remodeling following myocardial infarction, the process that forms the substrate for ventricular failure. The development of cell-based therapy as a strategy to repair or regenerate injured tissue offers extraordinary promise for a powerful anti-remodeling therapy. Various cell types, coming from various tissues may be used as therapy; among them, adipose-derived cells (CDTA). Methods: In this study, female rats were subjected to surgery to obtain the IM by ligation of the anterior coronary artery and treated with CDTA injected directly into the IM edge (Group T1) or injected intravenously into the tail vein (T2 group) or saline (untreated group) at 24 hours after MI to investigate the use of CDTA as cell-based therapy. Echocardiography (ECO) and electrocardiography (ECG) were performed before the IM and on days 1, 7 and 28 after IM. Results: The Q wave was present in D1 in all animals after IM at ECG exams. The QRS index (IQRS) was 1.342 ? 0.186 before the IM and 0.838 ? 0.15 after IM (N = 28, p <0.01). IQRS value increased in T1 group on days 7 and 28 after IM in contrast to day 1 (n = 6, p <0.001) and untreated group (n = 6, p <0.05). There were no improvements in IQRS at T2 group and untreated group. The average angle of the ventricular depolarization (AQRS) was 11.27 ? +60.45 degrees before IM and 26.01 ? +133.63 degrees after IM (N = 28; p <0.001). AQRS decreased in Group T1 on days 7 and 28 after MI as compared to day 1 (n = 6, p <0.01) and untreated group (N = 6, p <0.001). There was no improvement in AQRS in the T2 group and untreated group. The ejection fraction in M mode (FEM) measured by ECO was 92.16 ? 4.17% before IM and 53.24 ? 10.85% after IM (N = 6, p <0.001). The FEM in the T1 group increased on days 7 and 28 after MI compared to day 1 (n = 6, p <0.001) with similar values to those of the prior IM. The FEM in the untreated group was not improved on days 7 and 28 after MI. There was no increase in left ventricular end-diastolic diameter (DDF) in T1 group after MI, while DDF was increased in the untreated group after IM. Ejection fraction by Simpson (FES), shortening fraction (FE) and cardiac output (DC) were improved in group T1 but not in T2 group and untreated group. Conclusion: CDTA injected on the edge of IM improved systolic function of animals and CDTA injected intravenously did not have the same effect.