Compatibilidade de Beauveria sp. e Metarhizium sp. com ?leo essencial da esp?cie vegetal Schinus molle L. e efic?cia no controle de larvas de Aedes aegypti

Detalhes bibliográficos
Autor(a) principal: Faria, Fernanda Sousa
Data de Publicação: 2019
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da UFRRJ
Texto Completo: https://tede.ufrrj.br/jspui/handle/jspui/2345
Resumo: The present study evaluated the compatibility between the CG 206 of Beauveria sp. and CG 153 Metarhizium sp. Isolates associated with the essential oil of Schinus molle L. (aroeira-salsa) and its effectiveness in controlling A. aegypti larvae. For compatibility between fungi and oil, they were evaluated as colony forming units (CFU) and colony diameter evaluation. Fifty microliters of oil and non-oil suspensions (at concentration 75 and 25ppm) at 1.1 ? 103 and 1.3 ? 103 conidia mL-1, respectively, spread on five identified plates BDEL + chloramphenicol (0.5%) and incubated (25 ? 1 ?C and RH?80%). After seven days or number of colonies was quantified. To evaluate the colony diameter, ten microliters of oil-free and 25ppm oil suspension were inoculated into the center of Petri dishes containing BDEL + chloramphenicol (0.5%) and incubated (25o ? 1 ?C and RH?80%). Inoculum points were measured for 7 days. In the S. molle efficacy evaluation test, larvae (L2) were exposed to 22.5 mL of EO at ratings 25, 50, 75 and 100ppm and the control group was immersed in sterile chlorinated water +tween 80 where the survival rate was observed for seven days. In the biological assay with fungus and OE, Beauveria sp. CG 206 or Metarhizium sp. CG 153, grown in BDA, was suspended in sterile chlorinated water + tween 80 (0.01%) at a concentration of 1x107 conidial mL-1. For preparation of the oil-added fungal suspensions, each 10 ml fungal suspension received 0.0025% of the EO. Groups containing 15 larvae (N = 45) L2 were immersed in 22.5mL of suspensions. The control group was immersed in sterile chlorinated water + tween 80. A larval survival rate was evaluated daily for seven days. CFU tests and colony diameter were submitted to the Mann-Whitney test. Biological assays were submitted to nonparametric analysis (Kruskal-Wallis), followed by SNK test (Student-Newman-Keuls). Survival curve and survival averaging (S50) was performed using Kaplan-Meier. The significance level was 5% (P?0.05). In CFU tests, 75 ppm and 25 ppm decreases the number of colonies formed; CFU percentages were 72.33% (CG 153 + 75ppm), 43.92% (CG 206 + 75ppm), 67.96% (CG 153 + 25ppm) and 27.55% (CG 206 + 25ppm). In the evaluation of colony growth, there was no difference between oil and non-oil suspensions of both isolates. In no efficacy test of S. molle EO, the oil was effective in the 50, 75 and 100 ppm tests and a 25 ppm was chosen to compose it as suspensions, for the largest percentage of larvae duration (88.26%). In biological assays, as described suspensions, the best results were observed in the treatments with CG 153 Metarhizium sp. 107 conidia mL-1 associated or not with oil, where the larvae S50 was 1 day. It is concluded that both fungi and essential oil are compatible and, although there is no adjuvant action, are good options for controlling A. aegypti larvae.
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spelling Angelo, Isabele da Costa090.347.797-18Camargo, Mariana Guedes072.326.816-90Angelo, Isabele da CostaChaves, Douglas Siqueira de AlmeidaBezerra, Simone Quinelato132.955.637-21http://lattes.cnpq.br/8872370455608218Faria, Fernanda Sousa2022-07-12T16:28:20Z2019-08-27FARIA, Fernanda Sousa Compatibilidade de Beauveria sp. e Metarhizium sp. com ?leo essencial da esp?cie vegetal Schinus molle L. e efic?cia no controle de larvas de Aedes aegypti. 2019. 31 f. Disserta??o (Mestrado em Ci?ncias Veterin?rias) - Instituto de Veterin?ria, Universidade Federal Rural do Rio de Janeiro, Serop?dica, 2019.https://tede.ufrrj.br/jspui/handle/jspui/2345The present study evaluated the compatibility between the CG 206 of Beauveria sp. and CG 153 Metarhizium sp. Isolates associated with the essential oil of Schinus molle L. (aroeira-salsa) and its effectiveness in controlling A. aegypti larvae. For compatibility between fungi and oil, they were evaluated as colony forming units (CFU) and colony diameter evaluation. Fifty microliters of oil and non-oil suspensions (at concentration 75 and 25ppm) at 1.1 ? 103 and 1.3 ? 103 conidia mL-1, respectively, spread on five identified plates BDEL + chloramphenicol (0.5%) and incubated (25 ? 1 ?C and RH?80%). After seven days or number of colonies was quantified. To evaluate the colony diameter, ten microliters of oil-free and 25ppm oil suspension were inoculated into the center of Petri dishes containing BDEL + chloramphenicol (0.5%) and incubated (25o ? 1 ?C and RH?80%). Inoculum points were measured for 7 days. In the S. molle efficacy evaluation test, larvae (L2) were exposed to 22.5 mL of EO at ratings 25, 50, 75 and 100ppm and the control group was immersed in sterile chlorinated water +tween 80 where the survival rate was observed for seven days. In the biological assay with fungus and OE, Beauveria sp. CG 206 or Metarhizium sp. CG 153, grown in BDA, was suspended in sterile chlorinated water + tween 80 (0.01%) at a concentration of 1x107 conidial mL-1. For preparation of the oil-added fungal suspensions, each 10 ml fungal suspension received 0.0025% of the EO. Groups containing 15 larvae (N = 45) L2 were immersed in 22.5mL of suspensions. The control group was immersed in sterile chlorinated water + tween 80. A larval survival rate was evaluated daily for seven days. CFU tests and colony diameter were submitted to the Mann-Whitney test. Biological assays were submitted to nonparametric analysis (Kruskal-Wallis), followed by SNK test (Student-Newman-Keuls). Survival curve and survival averaging (S50) was performed using Kaplan-Meier. The significance level was 5% (P?0.05). In CFU tests, 75 ppm and 25 ppm decreases the number of colonies formed; CFU percentages were 72.33% (CG 153 + 75ppm), 43.92% (CG 206 + 75ppm), 67.96% (CG 153 + 25ppm) and 27.55% (CG 206 + 25ppm). In the evaluation of colony growth, there was no difference between oil and non-oil suspensions of both isolates. In no efficacy test of S. molle EO, the oil was effective in the 50, 75 and 100 ppm tests and a 25 ppm was chosen to compose it as suspensions, for the largest percentage of larvae duration (88.26%). In biological assays, as described suspensions, the best results were observed in the treatments with CG 153 Metarhizium sp. 107 conidia mL-1 associated or not with oil, where the larvae S50 was 1 day. It is concluded that both fungi and essential oil are compatible and, although there is no adjuvant action, are good options for controlling A. aegypti larvae.O presente estudo avaliou a compatibilidade entre os isolados CG 206 de Beauveria sp. e CG 153 de Metarhizium sp. associados ao ?leo essencial de Schinus molle L. (aroeira-salsa) e sua efic?cia no controle de larvas de A. aegypti. Para compatibilidade entre fungos e ?leo, foram avaliadas as unidades formadoras de col?nia (UFC) e avalia??o de di?metro de col?nia. Cinquenta microlitros de suspens?es com e sem ?leo (nas concentra??es de 75 e 25ppm) a 1,1?103 e 1,3?103 con?dios mL-1 respectivamente foram espalhados em cinco placas contendo BDEL+ cloranfenicol (0,5%) e incubado (25o?1?C e UR?80%). Ap?s sete dias o n?mero de col?nias foi quantificado. Para avalia??o de di?metro de col?nia, dez microlitros das suspens?es sem e com ?leo a 25ppm foram inoculados no centro de placas de Petri contendo BDEL+cloranfenicol (0,5%) e incubados (25o?1?C e UR?80%). Os pontos de in?culo, foram mensurados durante 7 dias. No teste de avalia??o de efic?cia de S. molle as larvas (L2) foram expostas a 22,5 mL do OE nas concentra??es 25, 50, 75 e 100ppm e o grupo controle foi imerso em em ?gua desclorada est?ril + tween 80, onde observou-se a taxa de sobreviv?ncia durante sete dias. No ensaio biol?gico com fungo e OE, con?dios de Beauveria sp. CG 206 ou Metarhizium sp. CG 153, cultivados em BDA, foram suspensos em ?gua desclorada est?ril + tween 80 (0,01%) na concentra??o de 1x107 con?dios mL-1. Para prepara??o das suspens?es f?ngicas acrescidas de ?leo, cada 10 mL de suspens?o f?ngica recebeu 0,0025% do OE. Grupos contendo 15 larvas (N=45) L2 foram imersas em 22,5mL das suspens?es. O grupo controle foi imerso em ?gua desclorada est?ril+tween 80. A taxa de sobreviv?ncia das larvas foi avaliada diariamente por sete dias. Os testes de UFC e di?metro de col?nia foram submetidos ao teste de Mann-Whitney. Os ensaios biol?gicos foram submetidos an?lise n?o param?trica (Kruskal-Wallis), seguido do teste SNK (Student-Newman-Keuls). A curva de sobreviv?ncia e o c?lculo da m?dia de sobreviv?ncia (S50) foi realizada utilizando Kaplan-Meier. O n?vel de signific?ncia foi de 5% (P?0,05). Nos testes da UFC observou-se que o OE a 75 ppm e 25 ppm diminu?ram o n?mero de col?nias formadas; a queda nos percentuais de UFC foram de 72,33% (CG 153+75ppm), 43,92% (CG 206+75ppm), 67,96% (CG 153+25ppm) e 27,55% (CG 206+25ppm). Na avalia??o do crescimento de col?nia n?o houve diferen?a entre suspens?es com e sem ?leo de ambos os isolados. No teste de efic?cia do OE de S. molle, o ?leo foi eficaz nas concentra??es de 50, 75 e 100 ppm e, a 25ppm foi escolhido para compor as suspens?es, pelo alto percentual de sobreviv?ncia dar larvas (88,26%). Nos ensaios biol?gicos, as suspens?es apresentaram efic?cia, sendo os melhores resultados observados nos tratamentos com o isolado CG 153 de Metarhizium sp. 107 con?dios mL-1 associado ou n?o ao ?leo, onde obteve-se o S50 das larvas de 1 dia. Conclui-se que ambos fungos e o ?leo essencial s?o compat?veis e, apesar de n?o haver a??o adjuvante, s?o boas op??es para controle de larvas de A. aegypti.Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2022-07-12T16:28:20Z No. of bitstreams: 1 2019 - Fernanda Sousa Faria.pdfMade available in DSpace on 2022-07-12T16:28:20Z (GMT). No. of bitstreams: 1 2019 - Fernanda Sousa Faria.pdfapplication/pdfhttps://tede.ufrrj.br/retrieve/69888/2019%20-%20Fernanda%20Sousa%20Faria.pdf.jpgporUniversidade Federal Rural do Rio de JaneiroPrograma de P?s-Gradua??o em Ci?ncias Veterin?riasUFRRJBrasilInstituto de Veterin?riaALKHAIBARI, A. M.; CAROLINO A. T.; J. C.; SAMUELS R. I.; BUTT T. M.; Differential Pathogenicity of Metarhizium blastospores and conidia against larvae of three mosquito species. J Med Entomol. v.54 n.3 p 696?704 mai. 2017. ALVES, S. B.; Fungos entomopatog?nicos. In: Alves, S.B. (Ed.), Controle microbiano de insetos. FEALQ: Piracicaba,289?382p. 1998. ARA?JO JR, J. M.; MARQUES, E. 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Oswaldo Cruz, v. 110, n. 4, p. 569-572, jun. 2015.mosquitosfungos entomopatogenicos?leo essencialmosquitoesentomopathogenic fungiessential oilMedicina Veterin?riaCompatibilidade de Beauveria sp. e Metarhizium sp. com ?leo essencial da esp?cie vegetal Schinus molle L. e efic?cia no controle de larvas de Aedes aegyptiCompatibility of Beauveria sp. and Metarhizium sp. with essential oil of the plant species Schinus molle L. and efficiency in the control of Aedes aegypti larvaeinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UFRRJinstname:Universidade Federal Rural do Rio de Janeiro (UFRRJ)instacron:UFRRJTHUMBNAIL2019 - Fernanda Sousa Faria.pdf.jpg2019 - Fernanda Sousa Faria.pdf.jpgimage/jpeg1943http://localhost:8080/tede/bitstream/jspui/2345/3/2019+-+Fernanda+Sousa+Faria.pdf.jpgcc73c4c239a4c332d642ba1e7c7a9fb2MD53TEXT2019 - Fernanda Sousa Faria.pdf.txt2019 - Fernanda Sousa Faria.pdf.txttext/plain95953http://localhost:8080/tede/bitstream/jspui/2345/2/2019+-+Fernanda+Sousa+Faria.pdf.txt0800cf94798740d7880da85a9edf64ccMD52ORIGINAL2019 - Fernanda Sousa Faria.pdf2019 - Fernanda Sousa Faria.pdfapplication/pdf1757490http://localhost:8080/tede/bitstream/jspui/2345/1/2019+-+Fernanda+Sousa+Faria.pdf76bb4c4d670abad9958daec72dc32310MD51jspui/23452022-07-13 01:00:11.925oai:localhost:jspui/2345Biblioteca Digital de Teses e Dissertaçõeshttps://tede.ufrrj.br/PUBhttps://tede.ufrrj.br/oai/requestbibliot@ufrrj.br||bibliot@ufrrj.bropendoar:2022-07-13T04:00:11Biblioteca Digital de Teses e Dissertações da UFRRJ - Universidade Federal Rural do Rio de Janeiro (UFRRJ)false
dc.title.por.fl_str_mv Compatibilidade de Beauveria sp. e Metarhizium sp. com ?leo essencial da esp?cie vegetal Schinus molle L. e efic?cia no controle de larvas de Aedes aegypti
dc.title.alternative.eng.fl_str_mv Compatibility of Beauveria sp. and Metarhizium sp. with essential oil of the plant species Schinus molle L. and efficiency in the control of Aedes aegypti larvae
title Compatibilidade de Beauveria sp. e Metarhizium sp. com ?leo essencial da esp?cie vegetal Schinus molle L. e efic?cia no controle de larvas de Aedes aegypti
spellingShingle Compatibilidade de Beauveria sp. e Metarhizium sp. com ?leo essencial da esp?cie vegetal Schinus molle L. e efic?cia no controle de larvas de Aedes aegypti
Faria, Fernanda Sousa
mosquitos
fungos entomopatogenicos
?leo essencial
mosquitoes
entomopathogenic fungi
essential oil
Medicina Veterin?ria
title_short Compatibilidade de Beauveria sp. e Metarhizium sp. com ?leo essencial da esp?cie vegetal Schinus molle L. e efic?cia no controle de larvas de Aedes aegypti
title_full Compatibilidade de Beauveria sp. e Metarhizium sp. com ?leo essencial da esp?cie vegetal Schinus molle L. e efic?cia no controle de larvas de Aedes aegypti
title_fullStr Compatibilidade de Beauveria sp. e Metarhizium sp. com ?leo essencial da esp?cie vegetal Schinus molle L. e efic?cia no controle de larvas de Aedes aegypti
title_full_unstemmed Compatibilidade de Beauveria sp. e Metarhizium sp. com ?leo essencial da esp?cie vegetal Schinus molle L. e efic?cia no controle de larvas de Aedes aegypti
title_sort Compatibilidade de Beauveria sp. e Metarhizium sp. com ?leo essencial da esp?cie vegetal Schinus molle L. e efic?cia no controle de larvas de Aedes aegypti
author Faria, Fernanda Sousa
author_facet Faria, Fernanda Sousa
author_role author
dc.contributor.advisor1.fl_str_mv Angelo, Isabele da Costa
dc.contributor.advisor1ID.fl_str_mv 090.347.797-18
dc.contributor.advisor-co1.fl_str_mv Camargo, Mariana Guedes
dc.contributor.advisor-co1ID.fl_str_mv 072.326.816-90
dc.contributor.referee1.fl_str_mv Angelo, Isabele da Costa
dc.contributor.referee2.fl_str_mv Chaves, Douglas Siqueira de Almeida
dc.contributor.referee3.fl_str_mv Bezerra, Simone Quinelato
dc.contributor.authorID.fl_str_mv 132.955.637-21
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/8872370455608218
dc.contributor.author.fl_str_mv Faria, Fernanda Sousa
contributor_str_mv Angelo, Isabele da Costa
Camargo, Mariana Guedes
Angelo, Isabele da Costa
Chaves, Douglas Siqueira de Almeida
Bezerra, Simone Quinelato
dc.subject.por.fl_str_mv mosquitos
fungos entomopatogenicos
?leo essencial
topic mosquitos
fungos entomopatogenicos
?leo essencial
mosquitoes
entomopathogenic fungi
essential oil
Medicina Veterin?ria
dc.subject.eng.fl_str_mv mosquitoes
entomopathogenic fungi
essential oil
dc.subject.cnpq.fl_str_mv Medicina Veterin?ria
description The present study evaluated the compatibility between the CG 206 of Beauveria sp. and CG 153 Metarhizium sp. Isolates associated with the essential oil of Schinus molle L. (aroeira-salsa) and its effectiveness in controlling A. aegypti larvae. For compatibility between fungi and oil, they were evaluated as colony forming units (CFU) and colony diameter evaluation. Fifty microliters of oil and non-oil suspensions (at concentration 75 and 25ppm) at 1.1 ? 103 and 1.3 ? 103 conidia mL-1, respectively, spread on five identified plates BDEL + chloramphenicol (0.5%) and incubated (25 ? 1 ?C and RH?80%). After seven days or number of colonies was quantified. To evaluate the colony diameter, ten microliters of oil-free and 25ppm oil suspension were inoculated into the center of Petri dishes containing BDEL + chloramphenicol (0.5%) and incubated (25o ? 1 ?C and RH?80%). Inoculum points were measured for 7 days. In the S. molle efficacy evaluation test, larvae (L2) were exposed to 22.5 mL of EO at ratings 25, 50, 75 and 100ppm and the control group was immersed in sterile chlorinated water +tween 80 where the survival rate was observed for seven days. In the biological assay with fungus and OE, Beauveria sp. CG 206 or Metarhizium sp. CG 153, grown in BDA, was suspended in sterile chlorinated water + tween 80 (0.01%) at a concentration of 1x107 conidial mL-1. For preparation of the oil-added fungal suspensions, each 10 ml fungal suspension received 0.0025% of the EO. Groups containing 15 larvae (N = 45) L2 were immersed in 22.5mL of suspensions. The control group was immersed in sterile chlorinated water + tween 80. A larval survival rate was evaluated daily for seven days. CFU tests and colony diameter were submitted to the Mann-Whitney test. Biological assays were submitted to nonparametric analysis (Kruskal-Wallis), followed by SNK test (Student-Newman-Keuls). Survival curve and survival averaging (S50) was performed using Kaplan-Meier. The significance level was 5% (P?0.05). In CFU tests, 75 ppm and 25 ppm decreases the number of colonies formed; CFU percentages were 72.33% (CG 153 + 75ppm), 43.92% (CG 206 + 75ppm), 67.96% (CG 153 + 25ppm) and 27.55% (CG 206 + 25ppm). In the evaluation of colony growth, there was no difference between oil and non-oil suspensions of both isolates. In no efficacy test of S. molle EO, the oil was effective in the 50, 75 and 100 ppm tests and a 25 ppm was chosen to compose it as suspensions, for the largest percentage of larvae duration (88.26%). In biological assays, as described suspensions, the best results were observed in the treatments with CG 153 Metarhizium sp. 107 conidia mL-1 associated or not with oil, where the larvae S50 was 1 day. It is concluded that both fungi and essential oil are compatible and, although there is no adjuvant action, are good options for controlling A. aegypti larvae.
publishDate 2019
dc.date.issued.fl_str_mv 2019-08-27
dc.date.accessioned.fl_str_mv 2022-07-12T16:28:20Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv FARIA, Fernanda Sousa Compatibilidade de Beauveria sp. e Metarhizium sp. com ?leo essencial da esp?cie vegetal Schinus molle L. e efic?cia no controle de larvas de Aedes aegypti. 2019. 31 f. Disserta??o (Mestrado em Ci?ncias Veterin?rias) - Instituto de Veterin?ria, Universidade Federal Rural do Rio de Janeiro, Serop?dica, 2019.
dc.identifier.uri.fl_str_mv https://tede.ufrrj.br/jspui/handle/jspui/2345
identifier_str_mv FARIA, Fernanda Sousa Compatibilidade de Beauveria sp. e Metarhizium sp. com ?leo essencial da esp?cie vegetal Schinus molle L. e efic?cia no controle de larvas de Aedes aegypti. 2019. 31 f. Disserta??o (Mestrado em Ci?ncias Veterin?rias) - Instituto de Veterin?ria, Universidade Federal Rural do Rio de Janeiro, Serop?dica, 2019.
url https://tede.ufrrj.br/jspui/handle/jspui/2345
dc.language.iso.fl_str_mv por
language por
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