Anatomia microsc?pica do p?ncreas e detec??o de c?lulas neuroend?crinas imunorreativas a insulina, glucagon, somatostatina, serotonina e polipept?deo pancre?tico de Phrynops geoffroanus (Schweigger, 1812) e Hydromedusa maximiliani (Mikan, 1820) (Testudines, Chelidae)

Lopes, Danielle Alcantara Vieira

Anatomia microsc?pica do p?ncreas e detec??o de c?lulas neuroend?crinas imunorreativas a insulina, glucagon, somatostatina, serotonina e polipept?deo pancre?tico de Phrynops geoffroanus (Schweigger, 1812) e Hydromedusa maximiliani (Mikan, 1820) (Testudines, Chelidae)

Detalhes bibliográficos
Autor(a) principal:	Lopes, Danielle Alcantara Vieira
Data de Publicação:	2017
Tipo de documento:	Tese
Idioma:	por
Título da fonte:	Biblioteca Digital de Teses e Dissertações da UFRRJ
Texto Completo:	https://tede.ufrrj.br/jspui/handle/jspui/4428
Resumo:	Comprehension of the physiological processes of vertebrates is essential to obtain data on the biology of the various species. The pancreas is a mixed gland that in involved in digestive and homeostatic processes. Understanding the morphology and physiology of the pancreas in all classes of vertebrates is fundamental to clarify this gland's function in the digestion of foods, to help shed light on nutritional factors that can improve the management and conditioning of species in their ecosystems. In the case of Brazilian species, this attention is particularly important, since many are classified as vulnerable by the IUCN (2017) due to anthropic alterations of their natural habitats. This article describes the histology and immunohistochemistry of the pancreas of two species of the Chelidae family. For this purpose, the pancreas of four specimens of Phrynops geoffroanus and four of Hydromedusa maximiliani that had been fixed in 10% formol and deposited in the collection of Juiz de Fora Federal University were routinely processed for histology to obtain sections with thickness of 5 ?m. The histology of the pancreas of P. geoffroanus and H. maximiliani is characterized by an endocrine portion dispersed among the pancreatic acini, forming or not forming endocrine groups similar to pancreatic islets. No connective tissue capsule is observed separating the pancreatic islets from the surrounding exocrine portion. The immunohistochemical analysis revealed four types of endocrine cells in P. geoffroanus: cells producing insulin, glucagon, somatostatin and pancreatic polypeptide. In H. maximiliani, only three cell types were found, producing insulin, glucagon and somatostatin. The cellular organization of the endocrine groups similar to pancreatic islets is different in the two species. In P. geoffroanus, the insulin-producing cells are positioned in the center, while those producing glucagon occupy the periphery. In turn, in H. maximiliani, these to cell types are interspersed, generally around the blood vessels. These observations will help understand the digestive physiology of the two species and provide data for comparative analyses with other freshwater turtles

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network_name_str	Biblioteca Digital de Teses e Dissertações da UFRRJ
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spelling	Pinheiro, Nadja Lima093.401.577-53http://lattes.cnpq.br/2704840396057478Nascimento, Aparecida Alves dohttp://lattes.cnpq.br/9303894913891155Pinheiro, Nadja LimaMendes, Rosa Maria MarcosSantos, Marcos Antonio Jos? dosSousa, Bernadete Maria deCosta, Valdemar da Silva114.534.167-57http://lattes.cnpq.br/1371072861201386Lopes, Danielle Alcantara Vieira2021-03-04T11:36:35Z2017-03-17LOPES, Danielle Alcantara Vieira. Anatomia microsc?pica do p?ncreas e detec??o de c?lulas neuroend?crinas imunorreativas a insulina, glucagon, somatostatina, serotonina e polipept?deo pancre?tico de Phrynops geoffroanus (Schweigger, 1812) e Hydromedusa maximiliani (Mikan, 1820) (Testudines, Chelidae). 2017. 86 f. Tese (Doutorado em Biologia Animal) - Instituto de Ci?ncias Biol?gicas e da Sa?de, Universidade Federal Rural do Rio de Janeiro, Serop?dica-RJ, 2017.https://tede.ufrrj.br/jspui/handle/jspui/4428Comprehension of the physiological processes of vertebrates is essential to obtain data on the biology of the various species. The pancreas is a mixed gland that in involved in digestive and homeostatic processes. Understanding the morphology and physiology of the pancreas in all classes of vertebrates is fundamental to clarify this gland's function in the digestion of foods, to help shed light on nutritional factors that can improve the management and conditioning of species in their ecosystems. In the case of Brazilian species, this attention is particularly important, since many are classified as vulnerable by the IUCN (2017) due to anthropic alterations of their natural habitats. This article describes the histology and immunohistochemistry of the pancreas of two species of the Chelidae family. For this purpose, the pancreas of four specimens of Phrynops geoffroanus and four of Hydromedusa maximiliani that had been fixed in 10% formol and deposited in the collection of Juiz de Fora Federal University were routinely processed for histology to obtain sections with thickness of 5 ?m. The histology of the pancreas of P. geoffroanus and H. maximiliani is characterized by an endocrine portion dispersed among the pancreatic acini, forming or not forming endocrine groups similar to pancreatic islets. No connective tissue capsule is observed separating the pancreatic islets from the surrounding exocrine portion. The immunohistochemical analysis revealed four types of endocrine cells in P. geoffroanus: cells producing insulin, glucagon, somatostatin and pancreatic polypeptide. In H. maximiliani, only three cell types were found, producing insulin, glucagon and somatostatin. The cellular organization of the endocrine groups similar to pancreatic islets is different in the two species. In P. geoffroanus, the insulin-producing cells are positioned in the center, while those producing glucagon occupy the periphery. In turn, in H. maximiliani, these to cell types are interspersed, generally around the blood vessels. These observations will help understand the digestive physiology of the two species and provide data for comparative analyses with other freshwater turtlesA compreens?o dos processos fisiol?gicos nos vertebrados ? essencial para obten??o de dados sobre a biologia das esp?cies. O p?ncreas ? uma gl?ndula mista que atua em processos digestivos e homeost?ticos. Entender a morfologia e fisiologia do p?ncreas em todas as classes de vertebrados ? fundamental para esclarecer a fun??o desta gl?ndula na digest?o dos alimentos, de modo a contribuir com fatores nutricionais e assim melhorar o manejo e condicionamento das esp?cies em seus ecossistemas. Tratando-se de esp?cies de quel?nios brasileiros, esta aten??o se redobra, uma vez que grande parte ? classificada como esp?cie vulner?vel pela IUCN (2017) devido ?s altera??es antr?picas sofridas por seus h?bitats naturais. Logo, este trabalho descreve a histologia e imuno-histoqu?mica do p?ncreas de duas esp?cies da fam?lia Chelidae. Para isso, o p?ncreas de quatro esp?cimes adultos de Phrynops geoffroanus e quatro esp?cimes de Hydromedusa maximiliani foram fixados em formol 10% e depositados na Universidade Federal de Juiz de Fora, foram processados por t?cnica histol?gica de rotina para obten??o de cortes de 5 ?m de espessura. A histologia do p?ncreas das esp?cies P. geoffroanus e H. maximiliani ? caracterizada por uma por??o end?crina dispersa entre os acinos pancre?ticos, formando ou n?o grupamentos end?crinos semelhantes a ilhotas pancre?ticas. N?o ? observada nenhuma c?psula de tecido conjuntivo separando as ilhotas pancre?ticas da por??o ex?crina circundante. O estudo imuno-histoqu?mico revelou quatro tipos de c?lulas end?crinas no p?ncreas de P. geoffroanus, c?lulas produtoras de insulina, glucagon, somatostatina e polipept?deo pancre?tico. Na esp?cie H. maximiliani tr?s tipos celulares foram encontrados, c?lulas produtoras de insulina, glucagon e somatostatina. A organiza??o celular dos grupamentos end?crinos semelhantes a ilhotas pancre?ticas ? diferente nas duas esp?cies estudadas. Em P. geoffroanus as c?lulas produtoras de insulina posicionam-se no centro enquanto as c?lulas produtoras de glucagon ocupam a periferia. J? na esp?cie H. maximiliani os dois tipos celulares se intercalam, geralmente ao redor de vasos sangu?neos. Este estudo ir? ajudar no entendimento da fisiologia digestiva da esp?cie investigada e fornecer? dados para an?lises comparativas com outros quel?nios de ?gua doceSubmitted by Celso Magalhaes (celsomagalhaes@ufrrj.br) on 2021-03-04T11:36:34Z No. of bitstreams: 1 2017 - Danielle Alcantara Vieira Lopes.pdf: 4510710 bytes, checksum: 7ad485c41063d049b6c43f6a09bacc31 (MD5)Made available in DSpace on 2021-03-04T11:36:35Z (GMT). No. of bitstreams: 1 2017 - Danielle Alcantara Vieira Lopes.pdf: 4510710 bytes, checksum: 7ad485c41063d049b6c43f6a09bacc31 (MD5) Previous issue date: 2017-03-17Coordena??o e Aperfei?oamento de Pessoal de N?vel Superior - CAPESapplication/pdfhttps://tede.ufrrj.br/retrieve/64190/2017%20-%20Danielle%20Alcantara%20Vieira%20Lopes.pdf.jpgporUniversidade Federal Rural do Rio de JaneiroPrograma de P?s-Gradua??o em Biologia AnimalUFRRJBrasilInstituto de Ci?ncias Biol?gicas e da Sa?deAGID, R.; DUGUY, R. & SAINT GIRONS, H. Variations in the blood sugar, in the liver glycogen and in the histological appearance of the pancreas, in Vipera aspis, during the annual cycle. J Radiol Electrol Med Nucl., v. 53, p. 807?824, 1961. AGULLEIRO, B.; AYALA, A. G. & ABAD, E. An lmmunocytochemical and Ultrastructural Study of the Endocrine Pancreas of Pseudemys scripta elegans (Chelonia). Gen. Comp. Endocrinol., v. 60, p. 95-103, 1985. AHLMAN, H. & NILSSON, O. The gut as the largest endocrine organ in the body. 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dc.title.por.fl_str_mv	Anatomia microsc?pica do p?ncreas e detec??o de c?lulas neuroend?crinas imunorreativas a insulina, glucagon, somatostatina, serotonina e polipept?deo pancre?tico de Phrynops geoffroanus (Schweigger, 1812) e Hydromedusa maximiliani (Mikan, 1820) (Testudines, Chelidae)
dc.title.alternative.eng.fl_str_mv	Microscopic anatomy of the pancreas and detection of immunoreactive neuroendocrine cells to insulin, glucagon, somatostatin, serotonin and pancreatic polypeptide of Phrynops geoffroanus (Schweigger, 1812) and Hydromedusa maximiliani (Mikan, 1825) (Testudines, Chelidae)
title	Anatomia microsc?pica do p?ncreas e detec??o de c?lulas neuroend?crinas imunorreativas a insulina, glucagon, somatostatina, serotonina e polipept?deo pancre?tico de Phrynops geoffroanus (Schweigger, 1812) e Hydromedusa maximiliani (Mikan, 1820) (Testudines, Chelidae)
spellingShingle	Anatomia microsc?pica do p?ncreas e detec??o de c?lulas neuroend?crinas imunorreativas a insulina, glucagon, somatostatina, serotonina e polipept?deo pancre?tico de Phrynops geoffroanus (Schweigger, 1812) e Hydromedusa maximiliani (Mikan, 1820) (Testudines, Chelidae) Lopes, Danielle Alcantara Vieira C?gados Histologia Imuno-histoqu?mica P?ncreas freshwater turtles Histology Immunohistochemistry Pancreas Biologia Geral
title_short	Anatomia microsc?pica do p?ncreas e detec??o de c?lulas neuroend?crinas imunorreativas a insulina, glucagon, somatostatina, serotonina e polipept?deo pancre?tico de Phrynops geoffroanus (Schweigger, 1812) e Hydromedusa maximiliani (Mikan, 1820) (Testudines, Chelidae)
title_full	Anatomia microsc?pica do p?ncreas e detec??o de c?lulas neuroend?crinas imunorreativas a insulina, glucagon, somatostatina, serotonina e polipept?deo pancre?tico de Phrynops geoffroanus (Schweigger, 1812) e Hydromedusa maximiliani (Mikan, 1820) (Testudines, Chelidae)
title_fullStr	Anatomia microsc?pica do p?ncreas e detec??o de c?lulas neuroend?crinas imunorreativas a insulina, glucagon, somatostatina, serotonina e polipept?deo pancre?tico de Phrynops geoffroanus (Schweigger, 1812) e Hydromedusa maximiliani (Mikan, 1820) (Testudines, Chelidae)
title_full_unstemmed	Anatomia microsc?pica do p?ncreas e detec??o de c?lulas neuroend?crinas imunorreativas a insulina, glucagon, somatostatina, serotonina e polipept?deo pancre?tico de Phrynops geoffroanus (Schweigger, 1812) e Hydromedusa maximiliani (Mikan, 1820) (Testudines, Chelidae)
title_sort	Anatomia microsc?pica do p?ncreas e detec??o de c?lulas neuroend?crinas imunorreativas a insulina, glucagon, somatostatina, serotonina e polipept?deo pancre?tico de Phrynops geoffroanus (Schweigger, 1812) e Hydromedusa maximiliani (Mikan, 1820) (Testudines, Chelidae)
author	Lopes, Danielle Alcantara Vieira
author_facet	Lopes, Danielle Alcantara Vieira
author_role	author
dc.contributor.advisor1.fl_str_mv	Pinheiro, Nadja Lima
dc.contributor.advisor1ID.fl_str_mv	093.401.577-53
dc.contributor.advisor1Lattes.fl_str_mv	http://lattes.cnpq.br/2704840396057478
dc.contributor.advisor-co1.fl_str_mv	Nascimento, Aparecida Alves do
dc.contributor.advisor-co1Lattes.fl_str_mv	http://lattes.cnpq.br/9303894913891155
dc.contributor.referee1.fl_str_mv	Pinheiro, Nadja Lima
dc.contributor.referee2.fl_str_mv	Mendes, Rosa Maria Marcos
dc.contributor.referee3.fl_str_mv	Santos, Marcos Antonio Jos? dos
dc.contributor.referee4.fl_str_mv	Sousa, Bernadete Maria de
dc.contributor.referee5.fl_str_mv	Costa, Valdemar da Silva
dc.contributor.authorID.fl_str_mv	114.534.167-57
dc.contributor.authorLattes.fl_str_mv	http://lattes.cnpq.br/1371072861201386
dc.contributor.author.fl_str_mv	Lopes, Danielle Alcantara Vieira
contributor_str_mv	Pinheiro, Nadja Lima Nascimento, Aparecida Alves do Pinheiro, Nadja Lima Mendes, Rosa Maria Marcos Santos, Marcos Antonio Jos? dos Sousa, Bernadete Maria de Costa, Valdemar da Silva
dc.subject.por.fl_str_mv	C?gados Histologia Imuno-histoqu?mica P?ncreas
topic	C?gados Histologia Imuno-histoqu?mica P?ncreas freshwater turtles Histology Immunohistochemistry Pancreas Biologia Geral
dc.subject.eng.fl_str_mv	freshwater turtles Histology Immunohistochemistry Pancreas
dc.subject.cnpq.fl_str_mv	Biologia Geral
description	Comprehension of the physiological processes of vertebrates is essential to obtain data on the biology of the various species. The pancreas is a mixed gland that in involved in digestive and homeostatic processes. Understanding the morphology and physiology of the pancreas in all classes of vertebrates is fundamental to clarify this gland's function in the digestion of foods, to help shed light on nutritional factors that can improve the management and conditioning of species in their ecosystems. In the case of Brazilian species, this attention is particularly important, since many are classified as vulnerable by the IUCN (2017) due to anthropic alterations of their natural habitats. This article describes the histology and immunohistochemistry of the pancreas of two species of the Chelidae family. For this purpose, the pancreas of four specimens of Phrynops geoffroanus and four of Hydromedusa maximiliani that had been fixed in 10% formol and deposited in the collection of Juiz de Fora Federal University were routinely processed for histology to obtain sections with thickness of 5 ?m. The histology of the pancreas of P. geoffroanus and H. maximiliani is characterized by an endocrine portion dispersed among the pancreatic acini, forming or not forming endocrine groups similar to pancreatic islets. No connective tissue capsule is observed separating the pancreatic islets from the surrounding exocrine portion. The immunohistochemical analysis revealed four types of endocrine cells in P. geoffroanus: cells producing insulin, glucagon, somatostatin and pancreatic polypeptide. In H. maximiliani, only three cell types were found, producing insulin, glucagon and somatostatin. The cellular organization of the endocrine groups similar to pancreatic islets is different in the two species. In P. geoffroanus, the insulin-producing cells are positioned in the center, while those producing glucagon occupy the periphery. In turn, in H. maximiliani, these to cell types are interspersed, generally around the blood vessels. These observations will help understand the digestive physiology of the two species and provide data for comparative analyses with other freshwater turtles
publishDate	2017
dc.date.issued.fl_str_mv	2017-03-17
dc.date.accessioned.fl_str_mv	2021-03-04T11:36:35Z
dc.type.status.fl_str_mv	info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv	info:eu-repo/semantics/doctoralThesis
format	doctoralThesis
status_str	publishedVersion
dc.identifier.citation.fl_str_mv	LOPES, Danielle Alcantara Vieira. Anatomia microsc?pica do p?ncreas e detec??o de c?lulas neuroend?crinas imunorreativas a insulina, glucagon, somatostatina, serotonina e polipept?deo pancre?tico de Phrynops geoffroanus (Schweigger, 1812) e Hydromedusa maximiliani (Mikan, 1820) (Testudines, Chelidae). 2017. 86 f. Tese (Doutorado em Biologia Animal) - Instituto de Ci?ncias Biol?gicas e da Sa?de, Universidade Federal Rural do Rio de Janeiro, Serop?dica-RJ, 2017.
dc.identifier.uri.fl_str_mv	https://tede.ufrrj.br/jspui/handle/jspui/4428
identifier_str_mv	LOPES, Danielle Alcantara Vieira. Anatomia microsc?pica do p?ncreas e detec??o de c?lulas neuroend?crinas imunorreativas a insulina, glucagon, somatostatina, serotonina e polipept?deo pancre?tico de Phrynops geoffroanus (Schweigger, 1812) e Hydromedusa maximiliani (Mikan, 1820) (Testudines, Chelidae). 2017. 86 f. Tese (Doutorado em Biologia Animal) - Instituto de Ci?ncias Biol?gicas e da Sa?de, Universidade Federal Rural do Rio de Janeiro, Serop?dica-RJ, 2017.
url	https://tede.ufrrj.br/jspui/handle/jspui/4428
dc.language.iso.fl_str_mv	por
language	por
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Anatomia microsc?pica do p?ncreas e detec??o de c?lulas neuroend?crinas imunorreativas a insulina, glucagon, somatostatina, serotonina e polipept?deo pancre?tico de Phrynops geoffroanus (Schweigger, 1812) e Hydromedusa maximiliani (Mikan, 1820) (Testudines, Chelidae)

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