Modula??o da microbiota col?nica e sanidade de latentes: Fatores prebi?ticos de leite e de virul?nica de microrganismos

Detalhes bibliográficos
Autor(a) principal: Oliveira, Geraldo dos Santos
Data de Publicação: 2011
Tipo de documento: Tese
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da UFRRJ
Texto Completo: https://tede.ufrrj.br/jspui/handle/jspui/3837
Resumo: The gut microbiota plays an important role in providing human health barrier to colonization of pathogens (competitive exclusion), to exert important metabolic functions and stimulate the immune system. Lactobacillus and Bifidobacterium are the two most important genera for the maintenance of health. The intestinal microbiota may be qualitatively and quantitatively altered by the type of food eaten, called nutritional modulation, and the use of antiinflammatory drugs, laxatives and antibiotics. The main objective of this study was to determine the influence of either type of feeding and delivery on both composition and evolution of the gut microbiota of Bifidobacterium spp., Lactobacillus spp. Candida spp. Clostidium spp., Escherichia coli, fungi and total aerobic and anaerobic, in infants. The influence of diet and type of delivery in 68 neonates aged between seven and 21 days was evaluated. It was found that the type of delivery influence counts of lactobacilli, which were higher in those born vaginally. Counts of lactobacilli in infants fed LPM and LM and born vaginally were significantly higher (p <0.05) than those born by caesarean, suggesting a possibility of transference of the micro biota from mother to child. Lactobacilli counts were below the detection level of the method (1x102 CFU/g) in 44% of individuals fed with BLH?s milk, born vaginally and in 81% of those born caesarean section. Yeasts were found in all samples, regardless of type of delivery or feeding in numbers ranging from 1x105 to 1x1011. Candida albicans (30%) and C. parapsilosis (28%) were predominant in both types of delivery, independently of type of feeding with the exception of the micro biota of infants who were born vaginally and were fed with LPM, when prevailed the yeast of the genus Trichosporon sp. There was no significant influence of type of delivery, in counts of anaerobic microbiota. Another objective was to detect the presence and identify the fungal microbiota of human milk and anatomical sites in women and infants. Eighty-one per cent of 64 samples were positive for the presence of fungi, with a prevalence of Candida albicans (73%) followed by Candida parapsilosis (15.4%). Similar profiles to those observed in the total samples were found in milk, breast and oral cavity, suggesting a relationship between the skin infection of the mother and the infant with the milk ingested. The virulence of Candida species isolated was determined by testing the production of proteases and 100% were strongly positive, indicating a high degree of infectivity. Afterwards, it was investigated by means of PCR and PFGE, the occurrence of E.coli enterophatogenic (EPEC) e enteraggregative (EAEC) virulence genes in 39 E. coli isolates from new-born children faecal samples and rectal swabs. The targets selected for EPEC were the genes encoding for intimin adherence protein (eae), adherence factor (EAF) plasmid, fimbriae bundle forming pili (bpf). The genes target for EAEC were the plasmid for aggregative adherence (pAA) and its regulon (AggR). Both EAEC virulence targets were identified in one of the samples (2,6%) in neonates fed with milk formula, prepared by the maternity personnel. It is concluded that besides the type of delivery and breast-feeding, another important determinant of colonization of the intestine of infants are the hygienic conditions in food preparation and in new-born cares. It should be taken corrective actions related to the conditions of manipulation of milk for children, and prioritize the supply of breast milk especially in the first days of life when the secretion of colostrum is rich in antibodies and phagocytes.
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spelling Luchese, Rosa Helena270.942.270-00http://lattes.cnpq.br/7341531211426066Baroni, Francisco de AssisOliveira, Rozely Zancop?Franco, Robson MaiaCampos, S?rgio GasparOliveira, Angela de632.187.207-53http://lattes.cnpq.br/7570954124455709Oliveira, Geraldo dos Santos2020-08-21T01:23:03Z2011-08-18OLIVEIRA, Geraldo dos Santos.Modula??o da microbiota col?nica e sanidade de latentes: Fatores prebi?ticos de leite e de virul?nica de microrganismos . 2011. 107 f. Tese (Programa de P?s-Gradua??o em Ci?ncia e Tecnologia de Alimentos) - Universidade Federal Rural do Rio de Janeiro, Serop?dica.https://tede.ufrrj.br/jspui/handle/jspui/3837The gut microbiota plays an important role in providing human health barrier to colonization of pathogens (competitive exclusion), to exert important metabolic functions and stimulate the immune system. Lactobacillus and Bifidobacterium are the two most important genera for the maintenance of health. The intestinal microbiota may be qualitatively and quantitatively altered by the type of food eaten, called nutritional modulation, and the use of antiinflammatory drugs, laxatives and antibiotics. The main objective of this study was to determine the influence of either type of feeding and delivery on both composition and evolution of the gut microbiota of Bifidobacterium spp., Lactobacillus spp. Candida spp. Clostidium spp., Escherichia coli, fungi and total aerobic and anaerobic, in infants. The influence of diet and type of delivery in 68 neonates aged between seven and 21 days was evaluated. It was found that the type of delivery influence counts of lactobacilli, which were higher in those born vaginally. Counts of lactobacilli in infants fed LPM and LM and born vaginally were significantly higher (p <0.05) than those born by caesarean, suggesting a possibility of transference of the micro biota from mother to child. Lactobacilli counts were below the detection level of the method (1x102 CFU/g) in 44% of individuals fed with BLH?s milk, born vaginally and in 81% of those born caesarean section. Yeasts were found in all samples, regardless of type of delivery or feeding in numbers ranging from 1x105 to 1x1011. Candida albicans (30%) and C. parapsilosis (28%) were predominant in both types of delivery, independently of type of feeding with the exception of the micro biota of infants who were born vaginally and were fed with LPM, when prevailed the yeast of the genus Trichosporon sp. There was no significant influence of type of delivery, in counts of anaerobic microbiota. Another objective was to detect the presence and identify the fungal microbiota of human milk and anatomical sites in women and infants. Eighty-one per cent of 64 samples were positive for the presence of fungi, with a prevalence of Candida albicans (73%) followed by Candida parapsilosis (15.4%). Similar profiles to those observed in the total samples were found in milk, breast and oral cavity, suggesting a relationship between the skin infection of the mother and the infant with the milk ingested. The virulence of Candida species isolated was determined by testing the production of proteases and 100% were strongly positive, indicating a high degree of infectivity. Afterwards, it was investigated by means of PCR and PFGE, the occurrence of E.coli enterophatogenic (EPEC) e enteraggregative (EAEC) virulence genes in 39 E. coli isolates from new-born children faecal samples and rectal swabs. The targets selected for EPEC were the genes encoding for intimin adherence protein (eae), adherence factor (EAF) plasmid, fimbriae bundle forming pili (bpf). The genes target for EAEC were the plasmid for aggregative adherence (pAA) and its regulon (AggR). Both EAEC virulence targets were identified in one of the samples (2,6%) in neonates fed with milk formula, prepared by the maternity personnel. It is concluded that besides the type of delivery and breast-feeding, another important determinant of colonization of the intestine of infants are the hygienic conditions in food preparation and in new-born cares. It should be taken corrective actions related to the conditions of manipulation of milk for children, and prioritize the supply of breast milk especially in the first days of life when the secretion of colostrum is rich in antibodies and phagocytes.A microbiota do intestino desempenha papel importante na sa?de humana propiciando barreira ? coloniza??o de pat?genos (exclus?o competitiva), por exercer fun??es metab?licas importantes, e por estimular o sistema imune. Bifidobacterium e Lactobacillus s?o os dois g?neros mais importantes para a manuten??o da sa?de. A microbiota intestinal poder? ser qualitativa e quantitativamente alterada pelo tipo de alimento ingerido, chamada modula??o nutricional, e pelo uso de anti-inflamat?rios, laxantes e antimicrobianos. O objetivo do trabalho foi avaliar a composi??o e evolu??o da microbiota intestinal de crian?as rec?m nascidas e relacionar ao tipo de amamenta??o, de parto, com a presen?a de Bifidobacterium spp., Lactobacillus spp., Clostridium spp., Escherichia coli e fungos, al?m da determina??o de aer?bios e anaer?bios totais. Foi avaliada a influencia da alimenta??o e do tipo de parto em 68 neonatos com idade entre sete e 21 dias. Verificou-se influ?ncia do tipo de parto nas contagens de lactobacilos, que foram maiores naqueles nascidos de parto normal. Contagens de lactobacilos em lactentes alimentados com leite em p? modificado (LPM) e leite do peito (LM) e nascidos de parto normal foram significativamente superiores (p<0,05) aquelas dos nascidos de parto cesariano, sugerindo uma possibilidade de transfer?ncia da microbiota da m?e para a crian?a. As contagens de lactobacilos estiveram abaixo do n?vel de detec??o do m?todo (1x102 UFC/g) em 44% dos indiv?duos alimentados com leite do banco de leite humano (BLH) e nascidas de parto normal e em 81% daquelas nascidas de parto ces?reo. Leveduras foram encontradas em todas as amostras, independentemente do tipo de parto ou aleitamento em n?meros que variaram de 1x105 a 1x1011. Candida albicans (30%) e C. parapsilosis (28%) predominaram em ambos os tipos de parto independemente do tipo de aleitamento com excess?o da microbiota de lactentes que receberam LPM nascidos de parto normal, quando predominou a levedura do g?nero Trichosporon sp. N?o foi observada influencia significativa do tipo de parto, nas contagens da microbiota anaer?bica. Outro objetivo do trabalho foi detectar a presen?a e identificar a microbiota f?ngica de leite humano e de s?tios anat?micos em mulheres e lactentes. A virul?ncia das linhagens de levedura encontradas foi avaliada atrav?s de testes de determina??o da atividade proteol?tica. De 64 amostras avaliadas, 81% foram positivas para a presen?a de fungos, com preval?ncia de Candida albicans (73%) seguida de Candida parapsilosis (15,4%). Perfis semelhantes aos verificados no total de amostras foram encontrados no leite, nas mamas e na cavidade oral, concluindo-se que existe a possibilidade da transfer?ncia da infec??o cut?nea da m?e para o lactante com o leite ingerido. A virul?ncia das esp?cies de Candida isoladas, foi determinada pelo teste de produ??o de proteases sendo que 100% foram fortemente positivas, indicando alto grau de infectividade. Posteriormente, investigou-se a presen?a de genes de virul?ncia de E. coli enteropatogenica (EPEC) e enteroagregativa (EAEC) em 39 isolados de amostras fecais e de swabs retais dos rec?m nascidos atrav?s das t?cnicas de polimerase chain reaction (PCR) multiplex e pulsed field gel eletrophoresis (PFGE). Os alvos selecionados para EPEC foram os genes que codificam a adesina intimina (eae), fator de ader?ncia (plasm?deo EAF) e f?mbria ?bundle forming pillus? (bpf). Os genes alvo para EAEC foram o plasm?deo de ader?ncia agregativa (pAA) e regulon (AggR). Foram identificados dois alvos de virul?ncia para EAEC em uma das amostras (2,6%) de neonatos alimentados com leite em p? modificado preparado em lact?rio. Conclui-se que al?m do tipo de parto e aleitamento um outro determinante importante da coloniza??o do intestino de lactentes sejam as condi??es higi?nicas no preparo da alimenta??o e nos cuidados com o neonato devendo ser tomadas medidas corretivas relacionadas ?s condi??es de manipula??o dos leites destinados as crian?as, al?m de priorizar a alimenta??o com leite do peito especialmente nos primeiros dias de vida quando ocorre a secre??o do colostro rico em anticorpos e fag?citos.Submitted by Sandra Pereira (srpereira@ufrrj.br) on 2020-08-21T01:23:03Z No. of bitstreams: 1 2011 - Geraldo dos Santos Oliveira.pdf: 4177922 bytes, checksum: 66a534f6b4f728d26ff56769cac9e981 (MD5)Made available in DSpace on 2020-08-21T01:23:03Z (GMT). No. of bitstreams: 1 2011 - Geraldo dos Santos Oliveira.pdf: 4177922 bytes, checksum: 66a534f6b4f728d26ff56769cac9e981 (MD5) Previous issue date: 2011-08-18application/pdfhttps://tede.ufrrj.br/retrieve/61696/2011%20-%20Geraldo%20dos%20Santos%20Oliveira.pdf.jpgporUniversidade Federal Rural do Rio de JaneiroPrograma de P?s-Gradua??o em Ci?ncia e Tecnologia de AlimentosUFRRJBrasilInstituto de TecnologiaALMEIDA, J.A.G. Qualidade do leite Humano coletado e processado em banco de leite. 1986. Disserta??o - Universidade Federal de Vi?osa, MG, 1986. ALVARES, C.; SUIDZZINSKI, T.E.; LOPES, M.E. Candidiese Vulvovaginal: Factores Predisponentes do Hospedeiro e virulencia. Rev. Bras.Patol. Med. Lab., 2007. 43(5) 19-327. AMIR, L.H.; PAKULA, S. Nipple pain, mastalgia and candid?asis in the lactating breast. Aus NZ ju obstet Gynaecol, v.31, n.4, p.378-380; 1991. AMIR, L.H. et al. Candida albicans: is it associated wit nipple pain in lacting women? Gynecol obstet Invest, v.41, n. 1, p. 30-34, 1996. 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C., GOMES, T. A., LIBERATORE, A. M., DE CASTRO, A. F. e ELIAS, W. P. Enteroaggregative Escherichia coli from humans and animals differ in major phenotypical traits and virulence genes. FEMS Microbiol Lett, v.256, n.2, Mar, p.251-7, 2006. UENO,M; JORGE,A.O.C.: Compara??o de T?cnicas Moleculares de An?lise de DNA Cromossomial destaphylococcus aureus resistentes a meticilina.Rev. 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dc.title.por.fl_str_mv Modula??o da microbiota col?nica e sanidade de latentes: Fatores prebi?ticos de leite e de virul?nica de microrganismos
dc.title.alternative.eng.fl_str_mv Potential prebiotic of human milk compared to milk powder modified in the modulation of colonic microbiota and sanity in infants.
title Modula??o da microbiota col?nica e sanidade de latentes: Fatores prebi?ticos de leite e de virul?nica de microrganismos
spellingShingle Modula??o da microbiota col?nica e sanidade de latentes: Fatores prebi?ticos de leite e de virul?nica de microrganismos
Oliveira, Geraldo dos Santos
Leite Humano
Leite Modificado
Prebi?tico
Bifidobacterium
Lactobacillus
Human Milk
Modified Milk Prebiotic
Bifidobacterium
Lactobacillus
Ci?ncia e Tecnologia de Alimentos
title_short Modula??o da microbiota col?nica e sanidade de latentes: Fatores prebi?ticos de leite e de virul?nica de microrganismos
title_full Modula??o da microbiota col?nica e sanidade de latentes: Fatores prebi?ticos de leite e de virul?nica de microrganismos
title_fullStr Modula??o da microbiota col?nica e sanidade de latentes: Fatores prebi?ticos de leite e de virul?nica de microrganismos
title_full_unstemmed Modula??o da microbiota col?nica e sanidade de latentes: Fatores prebi?ticos de leite e de virul?nica de microrganismos
title_sort Modula??o da microbiota col?nica e sanidade de latentes: Fatores prebi?ticos de leite e de virul?nica de microrganismos
author Oliveira, Geraldo dos Santos
author_facet Oliveira, Geraldo dos Santos
author_role author
dc.contributor.advisor1.fl_str_mv Luchese, Rosa Helena
dc.contributor.advisor1ID.fl_str_mv 270.942.270-00
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/7341531211426066
dc.contributor.advisor-co1.fl_str_mv Baroni, Francisco de Assis
dc.contributor.referee1.fl_str_mv Oliveira, Rozely Zancop?
dc.contributor.referee2.fl_str_mv Franco, Robson Maia
dc.contributor.referee3.fl_str_mv Campos, S?rgio Gaspar
dc.contributor.referee4.fl_str_mv Oliveira, Angela de
dc.contributor.authorID.fl_str_mv 632.187.207-53
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/7570954124455709
dc.contributor.author.fl_str_mv Oliveira, Geraldo dos Santos
contributor_str_mv Luchese, Rosa Helena
Baroni, Francisco de Assis
Oliveira, Rozely Zancop?
Franco, Robson Maia
Campos, S?rgio Gaspar
Oliveira, Angela de
dc.subject.por.fl_str_mv Leite Humano
Leite Modificado
Prebi?tico
Bifidobacterium
Lactobacillus
topic Leite Humano
Leite Modificado
Prebi?tico
Bifidobacterium
Lactobacillus
Human Milk
Modified Milk Prebiotic
Bifidobacterium
Lactobacillus
Ci?ncia e Tecnologia de Alimentos
dc.subject.eng.fl_str_mv Human Milk
Modified Milk Prebiotic
Bifidobacterium
Lactobacillus
dc.subject.cnpq.fl_str_mv Ci?ncia e Tecnologia de Alimentos
description The gut microbiota plays an important role in providing human health barrier to colonization of pathogens (competitive exclusion), to exert important metabolic functions and stimulate the immune system. Lactobacillus and Bifidobacterium are the two most important genera for the maintenance of health. The intestinal microbiota may be qualitatively and quantitatively altered by the type of food eaten, called nutritional modulation, and the use of antiinflammatory drugs, laxatives and antibiotics. The main objective of this study was to determine the influence of either type of feeding and delivery on both composition and evolution of the gut microbiota of Bifidobacterium spp., Lactobacillus spp. Candida spp. Clostidium spp., Escherichia coli, fungi and total aerobic and anaerobic, in infants. The influence of diet and type of delivery in 68 neonates aged between seven and 21 days was evaluated. It was found that the type of delivery influence counts of lactobacilli, which were higher in those born vaginally. Counts of lactobacilli in infants fed LPM and LM and born vaginally were significantly higher (p <0.05) than those born by caesarean, suggesting a possibility of transference of the micro biota from mother to child. Lactobacilli counts were below the detection level of the method (1x102 CFU/g) in 44% of individuals fed with BLH?s milk, born vaginally and in 81% of those born caesarean section. Yeasts were found in all samples, regardless of type of delivery or feeding in numbers ranging from 1x105 to 1x1011. Candida albicans (30%) and C. parapsilosis (28%) were predominant in both types of delivery, independently of type of feeding with the exception of the micro biota of infants who were born vaginally and were fed with LPM, when prevailed the yeast of the genus Trichosporon sp. There was no significant influence of type of delivery, in counts of anaerobic microbiota. Another objective was to detect the presence and identify the fungal microbiota of human milk and anatomical sites in women and infants. Eighty-one per cent of 64 samples were positive for the presence of fungi, with a prevalence of Candida albicans (73%) followed by Candida parapsilosis (15.4%). Similar profiles to those observed in the total samples were found in milk, breast and oral cavity, suggesting a relationship between the skin infection of the mother and the infant with the milk ingested. The virulence of Candida species isolated was determined by testing the production of proteases and 100% were strongly positive, indicating a high degree of infectivity. Afterwards, it was investigated by means of PCR and PFGE, the occurrence of E.coli enterophatogenic (EPEC) e enteraggregative (EAEC) virulence genes in 39 E. coli isolates from new-born children faecal samples and rectal swabs. The targets selected for EPEC were the genes encoding for intimin adherence protein (eae), adherence factor (EAF) plasmid, fimbriae bundle forming pili (bpf). The genes target for EAEC were the plasmid for aggregative adherence (pAA) and its regulon (AggR). Both EAEC virulence targets were identified in one of the samples (2,6%) in neonates fed with milk formula, prepared by the maternity personnel. It is concluded that besides the type of delivery and breast-feeding, another important determinant of colonization of the intestine of infants are the hygienic conditions in food preparation and in new-born cares. It should be taken corrective actions related to the conditions of manipulation of milk for children, and prioritize the supply of breast milk especially in the first days of life when the secretion of colostrum is rich in antibodies and phagocytes.
publishDate 2011
dc.date.issued.fl_str_mv 2011-08-18
dc.date.accessioned.fl_str_mv 2020-08-21T01:23:03Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv OLIVEIRA, Geraldo dos Santos.Modula??o da microbiota col?nica e sanidade de latentes: Fatores prebi?ticos de leite e de virul?nica de microrganismos . 2011. 107 f. Tese (Programa de P?s-Gradua??o em Ci?ncia e Tecnologia de Alimentos) - Universidade Federal Rural do Rio de Janeiro, Serop?dica.
dc.identifier.uri.fl_str_mv https://tede.ufrrj.br/jspui/handle/jspui/3837
identifier_str_mv OLIVEIRA, Geraldo dos Santos.Modula??o da microbiota col?nica e sanidade de latentes: Fatores prebi?ticos de leite e de virul?nica de microrganismos . 2011. 107 f. Tese (Programa de P?s-Gradua??o em Ci?ncia e Tecnologia de Alimentos) - Universidade Federal Rural do Rio de Janeiro, Serop?dica.
url https://tede.ufrrj.br/jspui/handle/jspui/3837
dc.language.iso.fl_str_mv por
language por
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