Adapta??o de metodologia de digest?o in vitro e determina??o da bioacessibilidade in vitro de Beta-caroteno em tr?s variedades de batata-doce de polpa alaranjada
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Data de Publicação: | 2010 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UFRRJ |
Texto Completo: | https://tede.ufrrj.br/jspui/handle/jspui/1186 |
Resumo: | Brazil was adverted by the World Health Organization (WHO) as a sub-clinical area of serious vitamin A deficiency. Thus, the Brazilian Agricultural Research Corporation (EMBRAPA) is selecting and improving varieties of sweet potatoes with higher levels of Beta-carotene, pro-vitamin A. The carotenoids provitamin A amount of in foods does not necessarily correspond the amount that is absorbed and metabolized by the body. For a better determination of these values and knowledge of the mechanisms of its transport and absorption, it is necessary to understand the factors that lead to the food matrix release, until the absorption and the influence on the promotion and maintenance in human health. In order to perform preliminary studies of its absorption, this study aims to determine the efficiency of micellization of Beta -carotene in orange sweet potato (Ipomoea batatas, Lam), by applying a in vitro digestion as a tool for determining the bioaccessibility, which is the first step for bioavailability determination. This approach aims to simulate the oral, gastric and intestinal stages of human. The digestion was performed with 10 g of fresh samples, homozeneided with 5% (w/w) of canola oil. The extraction was performed with acetone and petroleum ether and the micellar fraction with petroleum ether, NaCl 10% (w / v) and NaSO4 2% (w/v) and involves the use of enzymes as -amylase , pepsin, bile, pancreatin, lipase and mucin, and inorganic compounds such as KCl, KSCN, NaH2PO4, Na3PO4, NaOH, NaCl, CaCl2, HCl, NaHCO3. The physiological variations are reproduced by the heating bath shaker with orbital gyrus (37?C) and centrifugation (5000g, 45 min). Quantification and determination of the profile of carotenoids were performed by high performance liquid chromatography (HPLC) with YCM ? C30 Carotenoid S-3 4.6 x 250mm column. Quantification of total carotenoids was performed by UV-VIs. The whole procedure was performed under controlled temperature (25 ? C) and light. The Beta -carotene was present mainly with levels of 86%, 73% and 82% for access 1, 2 and 3 and after digestion, the profile of Beta -carotene has set levels of 96%, 89% and 100%, respectively. The efficiency of micellization was 23.8%, 28% and 25% for 1.2 and 3 hits, indicating Beta -carotene transfer of the food matrix to micelles, corresponding to bioaccessibility of the compound. This methodology proved to be faster and cheaper, since the in vivo studies are costly, complex and require more time. |
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Godoy, Ronoel Luiz de Oliveira507.802.047-00http://lattes.cnpq.br/3671854931659782Moura, Mirian Ribeiro LeiteAscheri, Jos? Luis Ram?rezBorguini, Renata Galhardo091.097.587-03http://lattes.cnpq.br/0043467331583018Giori, Fernanda Peixoto2016-08-30T14:26:59Z2010-02-23GIORI, Fernanda Peixoto. Adapta??o de metodologia de digest?o in vitro e determina??o da bioacessibilidade in vitro de beta-caroteno em tr?s variedades de batata-doce de polpa alaranjada. 2010. 68 f. Disserta??o (Mestrado em Ci?ncia e Tecnologia de Alimentos) - Instituto de Tecnologia, Universidade Federal Rural do Rio de Janeiro, Serop?dica, 2010.https://tede.ufrrj.br/jspui/handle/jspui/1186Brazil was adverted by the World Health Organization (WHO) as a sub-clinical area of serious vitamin A deficiency. Thus, the Brazilian Agricultural Research Corporation (EMBRAPA) is selecting and improving varieties of sweet potatoes with higher levels of Beta-carotene, pro-vitamin A. The carotenoids provitamin A amount of in foods does not necessarily correspond the amount that is absorbed and metabolized by the body. For a better determination of these values and knowledge of the mechanisms of its transport and absorption, it is necessary to understand the factors that lead to the food matrix release, until the absorption and the influence on the promotion and maintenance in human health. In order to perform preliminary studies of its absorption, this study aims to determine the efficiency of micellization of Beta -carotene in orange sweet potato (Ipomoea batatas, Lam), by applying a in vitro digestion as a tool for determining the bioaccessibility, which is the first step for bioavailability determination. This approach aims to simulate the oral, gastric and intestinal stages of human. The digestion was performed with 10 g of fresh samples, homozeneided with 5% (w/w) of canola oil. The extraction was performed with acetone and petroleum ether and the micellar fraction with petroleum ether, NaCl 10% (w / v) and NaSO4 2% (w/v) and involves the use of enzymes as -amylase , pepsin, bile, pancreatin, lipase and mucin, and inorganic compounds such as KCl, KSCN, NaH2PO4, Na3PO4, NaOH, NaCl, CaCl2, HCl, NaHCO3. The physiological variations are reproduced by the heating bath shaker with orbital gyrus (37?C) and centrifugation (5000g, 45 min). Quantification and determination of the profile of carotenoids were performed by high performance liquid chromatography (HPLC) with YCM ? C30 Carotenoid S-3 4.6 x 250mm column. Quantification of total carotenoids was performed by UV-VIs. The whole procedure was performed under controlled temperature (25 ? C) and light. The Beta -carotene was present mainly with levels of 86%, 73% and 82% for access 1, 2 and 3 and after digestion, the profile of Beta -carotene has set levels of 96%, 89% and 100%, respectively. The efficiency of micellization was 23.8%, 28% and 25% for 1.2 and 3 hits, indicating Beta -carotene transfer of the food matrix to micelles, corresponding to bioaccessibility of the compound. This methodology proved to be faster and cheaper, since the in vivo studies are costly, complex and require more time.O Brasil foi classificado pela Organiza??o Mundial da Sa?de (OMS) como ?rea de car?ncia sub-cl?nica grave de vitamina A. Assim, a Empresa Brasileira de Pesquisa Agropecu?ria (EMBRAPA) v?m selecionando e melhorando variedades de batata-doce com teores maiores de Beta-caroteno, composto pr?-vitamina A. A quantidade de caroten?ides pr?-vitamina A presentes nos alimentos n?o corresponde necessariamente ?quela quantidade absorvida e metabolizada pelo organismo. Para uma melhor determina??o destes valores e conhecimento dos mecanismos de transporte e absor??o deste composto, faz-se necess?rio, o entendimento dos fatores que levam ? sua libera??o da matriz do alimento, at? a extens?o de sua absor??o, bem como a influ?ncia na promo??o e manuten??o da sa?de humana. A fim de realizar estudos preliminares de sua absor??o, este trabalho visa determinar a efici?ncia de miceliza??o de Beta-caroteno de batata-doce de polpa alaranjada (Ipomoea batatas, Lam.), atrav?s da aplica??o de digest?o in vitro, como ferramenta de determina??o da bioacessibilidade, etapa preliminar para a determina??o da biodisponibilidade. Esta metodologia visa simular as etapas de digest?o oral, g?strica e intestinal humana. Foram pesados 10g de amostra in natura e adicionados 5% (p/p) de ?leo de canola. A extra??o do alimento foi realizada com acetona e ?ter de petr?leo e a da fra??o micelar, com ?ter de petr?leo, NaCl 10%(p/v) e NaSO4 2%(p/v) . Enzimas como: a-amilase, pepsina, bile, pancreatina, lipase e mucina, bem como compostos inorg?nicos, tais como KCl, KSCN, NaH2PO4, Na3PO4, NaOH, NaCl, CaCl2, HCl, NaHCO3. As varia??es fisiol?gicas foram reproduzidas pelo banho de aquecimento com giro orbital (37?C) e centrifuga??o (5000g;45 min). A quantifica??o e determina??o do perfil de caroten?ides foi realizada por cromatografia l?quida de alta efici?ncia (CLAE), com coluna YCM? Carotenoid C30 S-3 de 4,6 x 250mm. A quantifica??o de caroten?ides totais foi realizada por espectrofotometria UV-VIs. Todo o procedimento foi executado sob temperatura (25?C) e luz controlada. O Beta-caroteno estava presente majoritariamente, com teores de 86%, 73% e 82%, para as variedades 1, 2 e 3 e ap?s a digest?o, o perfil do Beta-caroteno passou a configurar teores de 96%, 89% e 100%, respectivamente. A efici?ncia de miceliza??o foi de 23,8%, 28% e 28,9% para as variedades 1,2 e 3, indicando a transfer?ncia do - caroteno da matriz do alimento para as micelas, correspondendo a bioacessibilidade deste composto. Esta metodologia demonstrou-se mais r?pida e mais barata, quando comparada aos estudos in vivo, que s?o mais onerosos, complexos e demandam mais tempo.Submitted by Sandra Pereira (srpereira@ufrrj.br) on 2016-08-30T14:26:59Z No. of bitstreams: 1 2010 - Fernanda Peixoto Giori.pdf: 2362278 bytes, checksum: a15d423533272c981b15d7f22b714be1 (MD5)Made available in DSpace on 2016-08-30T14:26:59Z (GMT). No. of bitstreams: 1 2010 - Fernanda Peixoto Giori.pdf: 2362278 bytes, checksum: a15d423533272c981b15d7f22b714be1 (MD5) Previous issue date: 2010-02-23CAPES - Coordena??o de Aperfei?oamento de Pessoal de N?vel Superiorapplication/pdfhttps://tede.ufrrj.br/retrieve/4656/2010%20-%20Fernanda%20Peixoto%20Giori.pdf.jpghttps://tede.ufrrj.br/retrieve/19284/2010%20-%20Fernanda%20Peixoto%20Giori.pdf.jpghttps://tede.ufrrj.br/retrieve/25627/2010%20-%20Fernanda%20Peixoto%20Giori.pdf.jpghttps://tede.ufrrj.br/retrieve/32054/2010%20-%20Fernanda%20Peixoto%20Giori.pdf.jpghttps://tede.ufrrj.br/retrieve/38422/2010%20-%20Fernanda%20Peixoto%20Giori.pdf.jpghttps://tede.ufrrj.br/retrieve/44818/2010%20-%20Fernanda%20Peixoto%20Giori.pdf.jpghttps://tede.ufrrj.br/retrieve/51228/2010%20-%20Fernanda%20Peixoto%20Giori.pdf.jpghttps://tede.ufrrj.br/retrieve/57684/2010%20-%20Fernanda%20Peixoto%20Giori.pdf.jpgporUniversidade Federal Rural do Rio de JaneiroPrograma de P?s-Gradua??o em Ci?ncia e Tecnologia de AlimentosUFRRJBrasilInstituto de TecnologiaAIRES, M. 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H.; WEST, C.E; WESTSTRATE, J.A.; HAUTVAST, J.G. Dietary factors that affect the bioavailability of carotenoids. The Journal of Nutrition, v.130, p.503- 506, 2000. WERTZ, K., SILER, U.; GORALCZYK, R. Lycopene: modes of action to promote prostate health. Arch. Biochem. Biophys, v.430, p. 127?134, 2004. WITTSIEPE, J.; SCHEY, P.; HACK, A.; SELENA, F.; WILHELM, M. Comparison of different digestive tract models for estimating bioaccessibility of polychlorinated dibenzo-pdioxans and dibenzofurans (PCPP/F) from red slag ? Kiesselrot?. Int. Journal Hyg. Environment, v. 203, p. 263-273, 2001. WORLD HEALTH ORGANIZATION. Indicators of assessing vitamin A deficiency and their application in monitoring and evaluating intervention programmes. Genova, 1996. Acesso em: 05 de setembro de 2009. Dispon?vel em: http://www. who.int YONEKURA, L.; NAGAO, A. Intestinal absorption of dietary carotenoids. Mol. Nutr. Food Res, v. 51, p.107-115, 2007. ZANATTA, CINTHIA FERNANDA. Determina??o da composi??o de caroten?ides e antocianinas de camu-camu. Disserta??o (mestrado). Universidade Estadual de Campinas. Faculdade de Engenharia de alimentos. 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dc.title.por.fl_str_mv |
Adapta??o de metodologia de digest?o in vitro e determina??o da bioacessibilidade in vitro de Beta-caroteno em tr?s variedades de batata-doce de polpa alaranjada |
dc.title.alternative.eng.fl_str_mv |
Adaptation of in vitro digestion methodology and determination of in vitro Beta-carotene bioacessibility of three orange sweet potato varieties |
title |
Adapta??o de metodologia de digest?o in vitro e determina??o da bioacessibilidade in vitro de Beta-caroteno em tr?s variedades de batata-doce de polpa alaranjada |
spellingShingle |
Adapta??o de metodologia de digest?o in vitro e determina??o da bioacessibilidade in vitro de Beta-caroteno em tr?s variedades de batata-doce de polpa alaranjada Giori, Fernanda Peixoto In vitro digestion HPLC Carotenoids Digest?o in vitro CLAE Caroten?ides Ci?ncia e Tecnologia de Alimentos |
title_short |
Adapta??o de metodologia de digest?o in vitro e determina??o da bioacessibilidade in vitro de Beta-caroteno em tr?s variedades de batata-doce de polpa alaranjada |
title_full |
Adapta??o de metodologia de digest?o in vitro e determina??o da bioacessibilidade in vitro de Beta-caroteno em tr?s variedades de batata-doce de polpa alaranjada |
title_fullStr |
Adapta??o de metodologia de digest?o in vitro e determina??o da bioacessibilidade in vitro de Beta-caroteno em tr?s variedades de batata-doce de polpa alaranjada |
title_full_unstemmed |
Adapta??o de metodologia de digest?o in vitro e determina??o da bioacessibilidade in vitro de Beta-caroteno em tr?s variedades de batata-doce de polpa alaranjada |
title_sort |
Adapta??o de metodologia de digest?o in vitro e determina??o da bioacessibilidade in vitro de Beta-caroteno em tr?s variedades de batata-doce de polpa alaranjada |
author |
Giori, Fernanda Peixoto |
author_facet |
Giori, Fernanda Peixoto |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Godoy, Ronoel Luiz de Oliveira |
dc.contributor.advisor1ID.fl_str_mv |
507.802.047-00 |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/3671854931659782 |
dc.contributor.referee1.fl_str_mv |
Moura, Mirian Ribeiro Leite |
dc.contributor.referee2.fl_str_mv |
Ascheri, Jos? Luis Ram?rez |
dc.contributor.referee3.fl_str_mv |
Borguini, Renata Galhardo |
dc.contributor.authorID.fl_str_mv |
091.097.587-03 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/0043467331583018 |
dc.contributor.author.fl_str_mv |
Giori, Fernanda Peixoto |
contributor_str_mv |
Godoy, Ronoel Luiz de Oliveira Moura, Mirian Ribeiro Leite Ascheri, Jos? Luis Ram?rez Borguini, Renata Galhardo |
dc.subject.eng.fl_str_mv |
In vitro digestion HPLC Carotenoids |
topic |
In vitro digestion HPLC Carotenoids Digest?o in vitro CLAE Caroten?ides Ci?ncia e Tecnologia de Alimentos |
dc.subject.por.fl_str_mv |
Digest?o in vitro CLAE Caroten?ides |
dc.subject.cnpq.fl_str_mv |
Ci?ncia e Tecnologia de Alimentos |
description |
Brazil was adverted by the World Health Organization (WHO) as a sub-clinical area of serious vitamin A deficiency. Thus, the Brazilian Agricultural Research Corporation (EMBRAPA) is selecting and improving varieties of sweet potatoes with higher levels of Beta-carotene, pro-vitamin A. The carotenoids provitamin A amount of in foods does not necessarily correspond the amount that is absorbed and metabolized by the body. For a better determination of these values and knowledge of the mechanisms of its transport and absorption, it is necessary to understand the factors that lead to the food matrix release, until the absorption and the influence on the promotion and maintenance in human health. In order to perform preliminary studies of its absorption, this study aims to determine the efficiency of micellization of Beta -carotene in orange sweet potato (Ipomoea batatas, Lam), by applying a in vitro digestion as a tool for determining the bioaccessibility, which is the first step for bioavailability determination. This approach aims to simulate the oral, gastric and intestinal stages of human. The digestion was performed with 10 g of fresh samples, homozeneided with 5% (w/w) of canola oil. The extraction was performed with acetone and petroleum ether and the micellar fraction with petroleum ether, NaCl 10% (w / v) and NaSO4 2% (w/v) and involves the use of enzymes as -amylase , pepsin, bile, pancreatin, lipase and mucin, and inorganic compounds such as KCl, KSCN, NaH2PO4, Na3PO4, NaOH, NaCl, CaCl2, HCl, NaHCO3. The physiological variations are reproduced by the heating bath shaker with orbital gyrus (37?C) and centrifugation (5000g, 45 min). Quantification and determination of the profile of carotenoids were performed by high performance liquid chromatography (HPLC) with YCM ? C30 Carotenoid S-3 4.6 x 250mm column. Quantification of total carotenoids was performed by UV-VIs. The whole procedure was performed under controlled temperature (25 ? C) and light. The Beta -carotene was present mainly with levels of 86%, 73% and 82% for access 1, 2 and 3 and after digestion, the profile of Beta -carotene has set levels of 96%, 89% and 100%, respectively. The efficiency of micellization was 23.8%, 28% and 25% for 1.2 and 3 hits, indicating Beta -carotene transfer of the food matrix to micelles, corresponding to bioaccessibility of the compound. This methodology proved to be faster and cheaper, since the in vivo studies are costly, complex and require more time. |
publishDate |
2010 |
dc.date.issued.fl_str_mv |
2010-02-23 |
dc.date.accessioned.fl_str_mv |
2016-08-30T14:26:59Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
GIORI, Fernanda Peixoto. Adapta??o de metodologia de digest?o in vitro e determina??o da bioacessibilidade in vitro de beta-caroteno em tr?s variedades de batata-doce de polpa alaranjada. 2010. 68 f. Disserta??o (Mestrado em Ci?ncia e Tecnologia de Alimentos) - Instituto de Tecnologia, Universidade Federal Rural do Rio de Janeiro, Serop?dica, 2010. |
dc.identifier.uri.fl_str_mv |
https://tede.ufrrj.br/jspui/handle/jspui/1186 |
identifier_str_mv |
GIORI, Fernanda Peixoto. Adapta??o de metodologia de digest?o in vitro e determina??o da bioacessibilidade in vitro de beta-caroteno em tr?s variedades de batata-doce de polpa alaranjada. 2010. 68 f. Disserta??o (Mestrado em Ci?ncia e Tecnologia de Alimentos) - Instituto de Tecnologia, Universidade Federal Rural do Rio de Janeiro, Serop?dica, 2010. |
url |
https://tede.ufrrj.br/jspui/handle/jspui/1186 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.references.por.fl_str_mv |
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Determina??o da composi??o de caroten?ides e antocianinas de camu-camu. Disserta??o (mestrado). Universidade Estadual de Campinas. Faculdade de Engenharia de alimentos. Campinas, S?o Paulo, 2004. |
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