Identification of immunodominant epitopes of Schistosoma mansoni vaccine candidate antigens using human T cells

Detalhes bibliográficos
Autor(a) principal: Fonseca, Cristina Toscano
Data de Publicação: 2004
Outros Autores: Cunha Neto, Edécio, Kalil, Jorge, Jesus, Amélia Maria Ribeiro de, Oliveira, Rodrigo Correa, Carvalho Filho, Edgar Marcelino de, Oliveira, Sérgio Costa
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFS
Texto Completo: https://ri.ufs.br/handle/riufs/922
Resumo: Paramyosin and Sm14 are two of the six antigens selected by the World Health Organization as candidates to compose a subunit vaccine against schistosomiasis. Both antigens are recognized by individuals naturally resistant to Schistosoma mansoni infection and induced protective immunity in the murine model. Three Sm14 epitopes and eleven paramyosin epitopes were selected by their ability to bind to different HLA-DR molecules using the TEPITOPE computer program, and these peptides were synthetically produced. The cellular recognition of Sm14 and paramyosin epitopes by peripheral blood mononuclear cells of individuals living in endemic area for schistosomiasis was tested by T cell proliferation assay. Among all Sm14 and paramyosin epitopes studied, Sm14-3 was preferentially recognized by individuals naturally resistant to S. mansoni infection while Para-5 was preferentially recognized by individuals resistant to reinfection. These two peptides represent promising antigens to be used in an experimental vaccine against schistosomiasis, since their preferential recognition by resistant individuals suggest their involvement in the induction of protective immunity.
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spelling Fonseca, Cristina ToscanoCunha Neto, EdécioKalil, JorgeJesus, Amélia Maria Ribeiro deOliveira, Rodrigo CorreaCarvalho Filho, Edgar Marcelino deOliveira, Sérgio Costa2014-02-20T00:25:48Z2014-02-20T00:25:48Z2004-08FONSECA, C. T. et al. Identification of immunodominant epitopes of Schistosoma mansoni vaccine candidate antigens using human T cells. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 99, supl. 1, ago. 2004. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762004000900011&lng=en&nrm=iso&tlng=en>. Acesso em: 19 fev. 2014.0074-0276https://ri.ufs.br/handle/riufs/922Creative Commons Attribution LicenseParamyosin and Sm14 are two of the six antigens selected by the World Health Organization as candidates to compose a subunit vaccine against schistosomiasis. Both antigens are recognized by individuals naturally resistant to Schistosoma mansoni infection and induced protective immunity in the murine model. Three Sm14 epitopes and eleven paramyosin epitopes were selected by their ability to bind to different HLA-DR molecules using the TEPITOPE computer program, and these peptides were synthetically produced. The cellular recognition of Sm14 and paramyosin epitopes by peripheral blood mononuclear cells of individuals living in endemic area for schistosomiasis was tested by T cell proliferation assay. Among all Sm14 and paramyosin epitopes studied, Sm14-3 was preferentially recognized by individuals naturally resistant to S. mansoni infection while Para-5 was preferentially recognized by individuals resistant to reinfection. These two peptides represent promising antigens to be used in an experimental vaccine against schistosomiasis, since their preferential recognition by resistant individuals suggest their involvement in the induction of protective immunity.Fundação Oswaldo CruzEsquistossomoSchistosoma mansoniEsquistossomoseAntígenosLinfócito TPeptídeos sintéticosParamiosinaIdentification of immunodominant epitopes of Schistosoma mansoni vaccine candidate antigens using human T cellsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleengreponame:Repositório Institucional da UFSinstname:Universidade Federal de Sergipe (UFS)instacron:UFSinfo:eu-repo/semantics/openAccessTHUMBNAILIdentificationSchistosomaMansoni.pdf.jpgIdentificationSchistosomaMansoni.pdf.jpgGenerated Thumbnailimage/jpeg1704https://ri.ufs.br/jspui/bitstream/riufs/922/4/IdentificationSchistosomaMansoni.pdf.jpgcb084c8b767948dbf8ccdc74457d00b3MD54ORIGINALIdentificationSchistosomaMansoni.pdfIdentificationSchistosomaMansoni.pdfapplication/pdf46024https://ri.ufs.br/jspui/bitstream/riufs/922/1/IdentificationSchistosomaMansoni.pdfef3ce24d1dc712452d4a886d664d5078MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://ri.ufs.br/jspui/bitstream/riufs/922/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52TEXTIdentificationSchistosomaMansoni.pdf.txtIdentificationSchistosomaMansoni.pdf.txtExtracted texttext/plain20770https://ri.ufs.br/jspui/bitstream/riufs/922/3/IdentificationSchistosomaMansoni.pdf.txt238983e896eb0b89289891b4c82f411dMD53riufs/9222014-02-20 02:00:17.106oai:ufs.br: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Repositório InstitucionalPUBhttps://ri.ufs.br/oai/requestrepositorio@academico.ufs.bropendoar:2014-02-20T05:00:17Repositório Institucional da UFS - Universidade Federal de Sergipe (UFS)false
dc.title.pt_BR.fl_str_mv Identification of immunodominant epitopes of Schistosoma mansoni vaccine candidate antigens using human T cells
title Identification of immunodominant epitopes of Schistosoma mansoni vaccine candidate antigens using human T cells
spellingShingle Identification of immunodominant epitopes of Schistosoma mansoni vaccine candidate antigens using human T cells
Fonseca, Cristina Toscano
Esquistossomo
Schistosoma mansoni
Esquistossomose
Antígenos
Linfócito T
Peptídeos sintéticos
Paramiosina
title_short Identification of immunodominant epitopes of Schistosoma mansoni vaccine candidate antigens using human T cells
title_full Identification of immunodominant epitopes of Schistosoma mansoni vaccine candidate antigens using human T cells
title_fullStr Identification of immunodominant epitopes of Schistosoma mansoni vaccine candidate antigens using human T cells
title_full_unstemmed Identification of immunodominant epitopes of Schistosoma mansoni vaccine candidate antigens using human T cells
title_sort Identification of immunodominant epitopes of Schistosoma mansoni vaccine candidate antigens using human T cells
author Fonseca, Cristina Toscano
author_facet Fonseca, Cristina Toscano
Cunha Neto, Edécio
Kalil, Jorge
Jesus, Amélia Maria Ribeiro de
Oliveira, Rodrigo Correa
Carvalho Filho, Edgar Marcelino de
Oliveira, Sérgio Costa
author_role author
author2 Cunha Neto, Edécio
Kalil, Jorge
Jesus, Amélia Maria Ribeiro de
Oliveira, Rodrigo Correa
Carvalho Filho, Edgar Marcelino de
Oliveira, Sérgio Costa
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Fonseca, Cristina Toscano
Cunha Neto, Edécio
Kalil, Jorge
Jesus, Amélia Maria Ribeiro de
Oliveira, Rodrigo Correa
Carvalho Filho, Edgar Marcelino de
Oliveira, Sérgio Costa
dc.subject.por.fl_str_mv Esquistossomo
Schistosoma mansoni
Esquistossomose
Antígenos
Linfócito T
Peptídeos sintéticos
Paramiosina
topic Esquistossomo
Schistosoma mansoni
Esquistossomose
Antígenos
Linfócito T
Peptídeos sintéticos
Paramiosina
description Paramyosin and Sm14 are two of the six antigens selected by the World Health Organization as candidates to compose a subunit vaccine against schistosomiasis. Both antigens are recognized by individuals naturally resistant to Schistosoma mansoni infection and induced protective immunity in the murine model. Three Sm14 epitopes and eleven paramyosin epitopes were selected by their ability to bind to different HLA-DR molecules using the TEPITOPE computer program, and these peptides were synthetically produced. The cellular recognition of Sm14 and paramyosin epitopes by peripheral blood mononuclear cells of individuals living in endemic area for schistosomiasis was tested by T cell proliferation assay. Among all Sm14 and paramyosin epitopes studied, Sm14-3 was preferentially recognized by individuals naturally resistant to S. mansoni infection while Para-5 was preferentially recognized by individuals resistant to reinfection. These two peptides represent promising antigens to be used in an experimental vaccine against schistosomiasis, since their preferential recognition by resistant individuals suggest their involvement in the induction of protective immunity.
publishDate 2004
dc.date.issued.fl_str_mv 2004-08
dc.date.accessioned.fl_str_mv 2014-02-20T00:25:48Z
dc.date.available.fl_str_mv 2014-02-20T00:25:48Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv FONSECA, C. T. et al. Identification of immunodominant epitopes of Schistosoma mansoni vaccine candidate antigens using human T cells. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 99, supl. 1, ago. 2004. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762004000900011&lng=en&nrm=iso&tlng=en>. Acesso em: 19 fev. 2014.
dc.identifier.uri.fl_str_mv https://ri.ufs.br/handle/riufs/922
dc.identifier.issn.none.fl_str_mv 0074-0276
dc.identifier.license.pt_BR.fl_str_mv Creative Commons Attribution License
identifier_str_mv FONSECA, C. T. et al. Identification of immunodominant epitopes of Schistosoma mansoni vaccine candidate antigens using human T cells. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 99, supl. 1, ago. 2004. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762004000900011&lng=en&nrm=iso&tlng=en>. Acesso em: 19 fev. 2014.
0074-0276
Creative Commons Attribution License
url https://ri.ufs.br/handle/riufs/922
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz
publisher.none.fl_str_mv Fundação Oswaldo Cruz
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFS
instname:Universidade Federal de Sergipe (UFS)
instacron:UFS
instname_str Universidade Federal de Sergipe (UFS)
instacron_str UFS
institution UFS
reponame_str Repositório Institucional da UFS
collection Repositório Institucional da UFS
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