Identification of immunodominant epitopes of Schistosoma mansoni vaccine candidate antigens using human T cells
Autor(a) principal: | |
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Data de Publicação: | 2004 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFS |
Texto Completo: | https://ri.ufs.br/handle/riufs/922 |
Resumo: | Paramyosin and Sm14 are two of the six antigens selected by the World Health Organization as candidates to compose a subunit vaccine against schistosomiasis. Both antigens are recognized by individuals naturally resistant to Schistosoma mansoni infection and induced protective immunity in the murine model. Three Sm14 epitopes and eleven paramyosin epitopes were selected by their ability to bind to different HLA-DR molecules using the TEPITOPE computer program, and these peptides were synthetically produced. The cellular recognition of Sm14 and paramyosin epitopes by peripheral blood mononuclear cells of individuals living in endemic area for schistosomiasis was tested by T cell proliferation assay. Among all Sm14 and paramyosin epitopes studied, Sm14-3 was preferentially recognized by individuals naturally resistant to S. mansoni infection while Para-5 was preferentially recognized by individuals resistant to reinfection. These two peptides represent promising antigens to be used in an experimental vaccine against schistosomiasis, since their preferential recognition by resistant individuals suggest their involvement in the induction of protective immunity. |
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Fonseca, Cristina ToscanoCunha Neto, EdécioKalil, JorgeJesus, Amélia Maria Ribeiro deOliveira, Rodrigo CorreaCarvalho Filho, Edgar Marcelino deOliveira, Sérgio Costa2014-02-20T00:25:48Z2014-02-20T00:25:48Z2004-08FONSECA, C. T. et al. Identification of immunodominant epitopes of Schistosoma mansoni vaccine candidate antigens using human T cells. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 99, supl. 1, ago. 2004. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762004000900011&lng=en&nrm=iso&tlng=en>. Acesso em: 19 fev. 2014.0074-0276https://ri.ufs.br/handle/riufs/922Creative Commons Attribution LicenseParamyosin and Sm14 are two of the six antigens selected by the World Health Organization as candidates to compose a subunit vaccine against schistosomiasis. Both antigens are recognized by individuals naturally resistant to Schistosoma mansoni infection and induced protective immunity in the murine model. Three Sm14 epitopes and eleven paramyosin epitopes were selected by their ability to bind to different HLA-DR molecules using the TEPITOPE computer program, and these peptides were synthetically produced. The cellular recognition of Sm14 and paramyosin epitopes by peripheral blood mononuclear cells of individuals living in endemic area for schistosomiasis was tested by T cell proliferation assay. Among all Sm14 and paramyosin epitopes studied, Sm14-3 was preferentially recognized by individuals naturally resistant to S. mansoni infection while Para-5 was preferentially recognized by individuals resistant to reinfection. These two peptides represent promising antigens to be used in an experimental vaccine against schistosomiasis, since their preferential recognition by resistant individuals suggest their involvement in the induction of protective immunity.Fundação Oswaldo CruzEsquistossomoSchistosoma mansoniEsquistossomoseAntígenosLinfócito TPeptídeos sintéticosParamiosinaIdentification of immunodominant epitopes of Schistosoma mansoni vaccine candidate antigens using human T cellsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleengreponame:Repositório Institucional da UFSinstname:Universidade Federal de Sergipe (UFS)instacron:UFSinfo:eu-repo/semantics/openAccessTHUMBNAILIdentificationSchistosomaMansoni.pdf.jpgIdentificationSchistosomaMansoni.pdf.jpgGenerated Thumbnailimage/jpeg1704https://ri.ufs.br/jspui/bitstream/riufs/922/4/IdentificationSchistosomaMansoni.pdf.jpgcb084c8b767948dbf8ccdc74457d00b3MD54ORIGINALIdentificationSchistosomaMansoni.pdfIdentificationSchistosomaMansoni.pdfapplication/pdf46024https://ri.ufs.br/jspui/bitstream/riufs/922/1/IdentificationSchistosomaMansoni.pdfef3ce24d1dc712452d4a886d664d5078MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://ri.ufs.br/jspui/bitstream/riufs/922/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52TEXTIdentificationSchistosomaMansoni.pdf.txtIdentificationSchistosomaMansoni.pdf.txtExtracted texttext/plain20770https://ri.ufs.br/jspui/bitstream/riufs/922/3/IdentificationSchistosomaMansoni.pdf.txt238983e896eb0b89289891b4c82f411dMD53riufs/9222014-02-20 02:00:17.106oai:ufs.br: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Repositório InstitucionalPUBhttps://ri.ufs.br/oai/requestrepositorio@academico.ufs.bropendoar:2014-02-20T05:00:17Repositório Institucional da UFS - Universidade Federal de Sergipe (UFS)false |
dc.title.pt_BR.fl_str_mv |
Identification of immunodominant epitopes of Schistosoma mansoni vaccine candidate antigens using human T cells |
title |
Identification of immunodominant epitopes of Schistosoma mansoni vaccine candidate antigens using human T cells |
spellingShingle |
Identification of immunodominant epitopes of Schistosoma mansoni vaccine candidate antigens using human T cells Fonseca, Cristina Toscano Esquistossomo Schistosoma mansoni Esquistossomose Antígenos Linfócito T Peptídeos sintéticos Paramiosina |
title_short |
Identification of immunodominant epitopes of Schistosoma mansoni vaccine candidate antigens using human T cells |
title_full |
Identification of immunodominant epitopes of Schistosoma mansoni vaccine candidate antigens using human T cells |
title_fullStr |
Identification of immunodominant epitopes of Schistosoma mansoni vaccine candidate antigens using human T cells |
title_full_unstemmed |
Identification of immunodominant epitopes of Schistosoma mansoni vaccine candidate antigens using human T cells |
title_sort |
Identification of immunodominant epitopes of Schistosoma mansoni vaccine candidate antigens using human T cells |
author |
Fonseca, Cristina Toscano |
author_facet |
Fonseca, Cristina Toscano Cunha Neto, Edécio Kalil, Jorge Jesus, Amélia Maria Ribeiro de Oliveira, Rodrigo Correa Carvalho Filho, Edgar Marcelino de Oliveira, Sérgio Costa |
author_role |
author |
author2 |
Cunha Neto, Edécio Kalil, Jorge Jesus, Amélia Maria Ribeiro de Oliveira, Rodrigo Correa Carvalho Filho, Edgar Marcelino de Oliveira, Sérgio Costa |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Fonseca, Cristina Toscano Cunha Neto, Edécio Kalil, Jorge Jesus, Amélia Maria Ribeiro de Oliveira, Rodrigo Correa Carvalho Filho, Edgar Marcelino de Oliveira, Sérgio Costa |
dc.subject.por.fl_str_mv |
Esquistossomo Schistosoma mansoni Esquistossomose Antígenos Linfócito T Peptídeos sintéticos Paramiosina |
topic |
Esquistossomo Schistosoma mansoni Esquistossomose Antígenos Linfócito T Peptídeos sintéticos Paramiosina |
description |
Paramyosin and Sm14 are two of the six antigens selected by the World Health Organization as candidates to compose a subunit vaccine against schistosomiasis. Both antigens are recognized by individuals naturally resistant to Schistosoma mansoni infection and induced protective immunity in the murine model. Three Sm14 epitopes and eleven paramyosin epitopes were selected by their ability to bind to different HLA-DR molecules using the TEPITOPE computer program, and these peptides were synthetically produced. The cellular recognition of Sm14 and paramyosin epitopes by peripheral blood mononuclear cells of individuals living in endemic area for schistosomiasis was tested by T cell proliferation assay. Among all Sm14 and paramyosin epitopes studied, Sm14-3 was preferentially recognized by individuals naturally resistant to S. mansoni infection while Para-5 was preferentially recognized by individuals resistant to reinfection. These two peptides represent promising antigens to be used in an experimental vaccine against schistosomiasis, since their preferential recognition by resistant individuals suggest their involvement in the induction of protective immunity. |
publishDate |
2004 |
dc.date.issued.fl_str_mv |
2004-08 |
dc.date.accessioned.fl_str_mv |
2014-02-20T00:25:48Z |
dc.date.available.fl_str_mv |
2014-02-20T00:25:48Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
FONSECA, C. T. et al. Identification of immunodominant epitopes of Schistosoma mansoni vaccine candidate antigens using human T cells. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 99, supl. 1, ago. 2004. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762004000900011&lng=en&nrm=iso&tlng=en>. Acesso em: 19 fev. 2014. |
dc.identifier.uri.fl_str_mv |
https://ri.ufs.br/handle/riufs/922 |
dc.identifier.issn.none.fl_str_mv |
0074-0276 |
dc.identifier.license.pt_BR.fl_str_mv |
Creative Commons Attribution License |
identifier_str_mv |
FONSECA, C. T. et al. Identification of immunodominant epitopes of Schistosoma mansoni vaccine candidate antigens using human T cells. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 99, supl. 1, ago. 2004. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762004000900011&lng=en&nrm=iso&tlng=en>. Acesso em: 19 fev. 2014. 0074-0276 Creative Commons Attribution License |
url |
https://ri.ufs.br/handle/riufs/922 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
dc.publisher.none.fl_str_mv |
Fundação Oswaldo Cruz |
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Fundação Oswaldo Cruz |
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reponame:Repositório Institucional da UFS instname:Universidade Federal de Sergipe (UFS) instacron:UFS |
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Universidade Federal de Sergipe (UFS) |
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UFS |
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