Identification of suitable reference genes for real time quantitative polymerase chain reaction assays on pectoralis major muscle in chicken (Gallus gallus)

Nascimento, Carlos Souza do; Barbosa, Leandro Teixeira; Brito, Claudson; Fernandes, Roberta Pereira Miranda; Silva-Mann, Renata; Pinto, Ana Paula Gomes; Oliveira, Haniel Cedraz de; Dodson, Mike V.; Guimarães, Simone Eliza Facioni; Duarte, Marcio de Souza

Identification of suitable reference genes for real time quantitative polymerase chain reaction assays on pectoralis major muscle in chicken (Gallus gallus)

Detalhes bibliográficos
Autor(a) principal:	Nascimento, Carlos Souza do
Data de Publicação:	2015
Outros Autores:	Barbosa, Leandro Teixeira, Brito, Claudson, Fernandes, Roberta Pereira Miranda, Silva-Mann, Renata, Pinto, Ana Paula Gomes, Oliveira, Haniel Cedraz de, Dodson, Mike V., Guimarães, Simone Eliza Facioni, Duarte, Marcio de Souza
Tipo de documento:	Artigo
Idioma:	por
Título da fonte:	Repositório Institucional da UFS
Texto Completo:	http://ri.ufs.br/jspui/handle/riufs/8493
Resumo:	Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPq

Metadados do item

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spelling	Nascimento, Carlos Souza doBarbosa, Leandro TeixeiraBrito, ClaudsonFernandes, Roberta Pereira MirandaSilva-Mann, RenataPinto, Ana Paula GomesOliveira, Haniel Cedraz deDodson, Mike V.Guimarães, Simone Eliza FacioniDuarte, Marcio de Souza2018-06-26T23:26:52Z2018-06-26T23:26:52Z2015-05NASCIMENTO, C. S. et al. Identification of suitable reference genes for real time quantitative polymerase chain reaction assays on pectoralis major muscle in chicken (Gallus gallus). PLOS One, 28 maio 2015. Disponível em: <https://doi.org/10.1371/journal.pone.0127935>. Acesso em: 26 jun. 2018.1932-6203http://ri.ufs.br/jspui/handle/riufs/8493Creative Commons Atribuição 4.0 Internacional (CC BY 4.0)Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPqCoordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESFundação de Apoio a Pesquisa e à Inovação Tecnológica do Estado de Sergipe - FAPITEC/SEThirteen reference genes were investigated to determine their stability to be used as a housekeeping in gene expression studies in skeletal muscle of chickens. Five different algorithms were used for ranking of reference genes and results suggested that individual rankings of the genes differed among them. The stability of the expression of reference genes were validated using samples obtained from the Pectoralis major muscle in chicken. Samples were obtained from chickens in different development periods post hatch and under different nutritional diets. For gene expression calculation the ΔΔCt approach was applied to compare relative expression of pairs of genes within each of 52 samples when normalized to mitochondrially encoded cytochrome c oxidase II (MT-CO2) target gene. Our findings showed that hydroxymethylbilane synthase (HMBS) and hypoxanthine phosphoribosyl transferase 1 (HPRT1) are the most stable reference genes while transferrin receptor (TFRC) and beta-2-microglobulin (B2M) ranked as the least stable genes in the Pectoralis major muscle of chickens. Moreover, our results revealed that HMBS and HPRT1 gene expression did not change due to dietary variations and thus it is recommended for accurate normalization of RT-qPCR data in chicken Pectoralis major muscle.porPLOSPLOS OneAvesGenesGallus gallusFrangosChickenIdentification of suitable reference genes for real time quantitative polymerase chain reaction assays on pectoralis major muscle in chicken (Gallus gallus)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlereponame:Repositório Institucional da UFSinstname:Universidade Federal de Sergipe (UFS)instacron:UFSinfo:eu-repo/semantics/openAccessTEXTIdentificationSuitableReference.PDF.txtIdentificationSuitableReference.PDF.txtExtracted texttext/plain43480https://ri.ufs.br/jspui/bitstream/riufs/8493/3/IdentificationSuitableReference.PDF.txt2bad0f1be5369397ae6723df49f9c9beMD53THUMBNAILIdentificationSuitableReference.PDF.jpgIdentificationSuitableReference.PDF.jpgGenerated Thumbnailimage/jpeg1767https://ri.ufs.br/jspui/bitstream/riufs/8493/4/IdentificationSuitableReference.PDF.jpg3e79a2c39be0510554e207d186d5b09eMD54LICENSElicense.txtlicense.txttext/plain; charset=utf-81475https://ri.ufs.br/jspui/bitstream/riufs/8493/1/license.txt098cbbf65c2c15e1fb2e49c5d306a44cMD51ORIGINALIdentificationSuitableReference.PDFIdentificationSuitableReference.PDFapplication/pdf1336715https://ri.ufs.br/jspui/bitstream/riufs/8493/2/IdentificationSuitableReference.PDFd6f666205ed6f51c76033b57fec9826dMD52riufs/84932018-06-26 20:27:40.442oai:ufs.br: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Repositório InstitucionalPUBhttps://ri.ufs.br/oai/requestrepositorio@academico.ufs.bropendoar:2018-06-26T23:27:40Repositório Institucional da UFS - Universidade Federal de Sergipe (UFS)false
dc.title.pt_BR.fl_str_mv	Identification of suitable reference genes for real time quantitative polymerase chain reaction assays on pectoralis major muscle in chicken (Gallus gallus)
title	Identification of suitable reference genes for real time quantitative polymerase chain reaction assays on pectoralis major muscle in chicken (Gallus gallus)
spellingShingle	Identification of suitable reference genes for real time quantitative polymerase chain reaction assays on pectoralis major muscle in chicken (Gallus gallus) Nascimento, Carlos Souza do Aves Genes Gallus gallus Frangos Chicken
title_short	Identification of suitable reference genes for real time quantitative polymerase chain reaction assays on pectoralis major muscle in chicken (Gallus gallus)
title_full	Identification of suitable reference genes for real time quantitative polymerase chain reaction assays on pectoralis major muscle in chicken (Gallus gallus)
title_fullStr	Identification of suitable reference genes for real time quantitative polymerase chain reaction assays on pectoralis major muscle in chicken (Gallus gallus)
title_full_unstemmed	Identification of suitable reference genes for real time quantitative polymerase chain reaction assays on pectoralis major muscle in chicken (Gallus gallus)
title_sort	Identification of suitable reference genes for real time quantitative polymerase chain reaction assays on pectoralis major muscle in chicken (Gallus gallus)
author	Nascimento, Carlos Souza do
author_facet	Nascimento, Carlos Souza do Barbosa, Leandro Teixeira Brito, Claudson Fernandes, Roberta Pereira Miranda Silva-Mann, Renata Pinto, Ana Paula Gomes Oliveira, Haniel Cedraz de Dodson, Mike V. Guimarães, Simone Eliza Facioni Duarte, Marcio de Souza
author_role	author
author2	Barbosa, Leandro Teixeira Brito, Claudson Fernandes, Roberta Pereira Miranda Silva-Mann, Renata Pinto, Ana Paula Gomes Oliveira, Haniel Cedraz de Dodson, Mike V. Guimarães, Simone Eliza Facioni Duarte, Marcio de Souza
author2_role	author author author author author author author author author
dc.contributor.author.fl_str_mv	Nascimento, Carlos Souza do Barbosa, Leandro Teixeira Brito, Claudson Fernandes, Roberta Pereira Miranda Silva-Mann, Renata Pinto, Ana Paula Gomes Oliveira, Haniel Cedraz de Dodson, Mike V. Guimarães, Simone Eliza Facioni Duarte, Marcio de Souza
dc.subject.por.fl_str_mv	Aves Genes Gallus gallus Frangos
topic	Aves Genes Gallus gallus Frangos Chicken
dc.subject.eng.fl_str_mv	Chicken
description	Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPq
publishDate	2015
dc.date.issued.fl_str_mv	2015-05
dc.date.accessioned.fl_str_mv	2018-06-26T23:26:52Z
dc.date.available.fl_str_mv	2018-06-26T23:26:52Z
dc.type.status.fl_str_mv	info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv	info:eu-repo/semantics/article
format	article
status_str	publishedVersion
dc.identifier.citation.fl_str_mv	NASCIMENTO, C. S. et al. Identification of suitable reference genes for real time quantitative polymerase chain reaction assays on pectoralis major muscle in chicken (Gallus gallus). PLOS One, 28 maio 2015. Disponível em: <https://doi.org/10.1371/journal.pone.0127935>. Acesso em: 26 jun. 2018.
dc.identifier.uri.fl_str_mv	http://ri.ufs.br/jspui/handle/riufs/8493
dc.identifier.issn.none.fl_str_mv	1932-6203
dc.identifier.license.pt_BR.fl_str_mv	Creative Commons Atribuição 4.0 Internacional (CC BY 4.0)
identifier_str_mv	NASCIMENTO, C. S. et al. Identification of suitable reference genes for real time quantitative polymerase chain reaction assays on pectoralis major muscle in chicken (Gallus gallus). PLOS One, 28 maio 2015. Disponível em: <https://doi.org/10.1371/journal.pone.0127935>. Acesso em: 26 jun. 2018. 1932-6203 Creative Commons Atribuição 4.0 Internacional (CC BY 4.0)
url	http://ri.ufs.br/jspui/handle/riufs/8493
dc.language.iso.fl_str_mv	por
language	por
dc.relation.ispartof.pt_BR.fl_str_mv	PLOS One
dc.rights.driver.fl_str_mv	info:eu-repo/semantics/openAccess
eu_rights_str_mv	openAccess
dc.publisher.none.fl_str_mv	PLOS
publisher.none.fl_str_mv	PLOS
dc.source.none.fl_str_mv	reponame:Repositório Institucional da UFS instname:Universidade Federal de Sergipe (UFS) instacron:UFS
instname_str	Universidade Federal de Sergipe (UFS)
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Identification of suitable reference genes for real time quantitative polymerase chain reaction assays on pectoralis major muscle in chicken (Gallus gallus)

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