Identification of suitable reference genes for real time quantitative polymerase chain reaction assays on pectoralis major muscle in chicken (Gallus gallus)
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFS |
Texto Completo: | http://ri.ufs.br/jspui/handle/riufs/8493 |
Resumo: | Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPq |
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Nascimento, Carlos Souza doBarbosa, Leandro TeixeiraBrito, ClaudsonFernandes, Roberta Pereira MirandaSilva-Mann, RenataPinto, Ana Paula GomesOliveira, Haniel Cedraz deDodson, Mike V.Guimarães, Simone Eliza FacioniDuarte, Marcio de Souza2018-06-26T23:26:52Z2018-06-26T23:26:52Z2015-05NASCIMENTO, C. S. et al. Identification of suitable reference genes for real time quantitative polymerase chain reaction assays on pectoralis major muscle in chicken (Gallus gallus). PLOS One, 28 maio 2015. Disponível em: <https://doi.org/10.1371/journal.pone.0127935>. Acesso em: 26 jun. 2018.1932-6203http://ri.ufs.br/jspui/handle/riufs/8493Creative Commons Atribuição 4.0 Internacional (CC BY 4.0)Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPqCoordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESFundação de Apoio a Pesquisa e à Inovação Tecnológica do Estado de Sergipe - FAPITEC/SEThirteen reference genes were investigated to determine their stability to be used as a housekeeping in gene expression studies in skeletal muscle of chickens. Five different algorithms were used for ranking of reference genes and results suggested that individual rankings of the genes differed among them. The stability of the expression of reference genes were validated using samples obtained from the Pectoralis major muscle in chicken. Samples were obtained from chickens in different development periods post hatch and under different nutritional diets. For gene expression calculation the ΔΔCt approach was applied to compare relative expression of pairs of genes within each of 52 samples when normalized to mitochondrially encoded cytochrome c oxidase II (MT-CO2) target gene. Our findings showed that hydroxymethylbilane synthase (HMBS) and hypoxanthine phosphoribosyl transferase 1 (HPRT1) are the most stable reference genes while transferrin receptor (TFRC) and beta-2-microglobulin (B2M) ranked as the least stable genes in the Pectoralis major muscle of chickens. Moreover, our results revealed that HMBS and HPRT1 gene expression did not change due to dietary variations and thus it is recommended for accurate normalization of RT-qPCR data in chicken Pectoralis major muscle.porPLOSPLOS OneAvesGenesGallus gallusFrangosChickenIdentification of suitable reference genes for real time quantitative polymerase chain reaction assays on pectoralis major muscle in chicken (Gallus gallus)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlereponame:Repositório Institucional da UFSinstname:Universidade Federal de Sergipe (UFS)instacron:UFSinfo:eu-repo/semantics/openAccessTEXTIdentificationSuitableReference.PDF.txtIdentificationSuitableReference.PDF.txtExtracted texttext/plain43480https://ri.ufs.br/jspui/bitstream/riufs/8493/3/IdentificationSuitableReference.PDF.txt2bad0f1be5369397ae6723df49f9c9beMD53THUMBNAILIdentificationSuitableReference.PDF.jpgIdentificationSuitableReference.PDF.jpgGenerated Thumbnailimage/jpeg1767https://ri.ufs.br/jspui/bitstream/riufs/8493/4/IdentificationSuitableReference.PDF.jpg3e79a2c39be0510554e207d186d5b09eMD54LICENSElicense.txtlicense.txttext/plain; charset=utf-81475https://ri.ufs.br/jspui/bitstream/riufs/8493/1/license.txt098cbbf65c2c15e1fb2e49c5d306a44cMD51ORIGINALIdentificationSuitableReference.PDFIdentificationSuitableReference.PDFapplication/pdf1336715https://ri.ufs.br/jspui/bitstream/riufs/8493/2/IdentificationSuitableReference.PDFd6f666205ed6f51c76033b57fec9826dMD52riufs/84932018-06-26 20:27:40.442oai:ufs.br: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Repositório InstitucionalPUBhttps://ri.ufs.br/oai/requestrepositorio@academico.ufs.bropendoar:2018-06-26T23:27:40Repositório Institucional da UFS - Universidade Federal de Sergipe (UFS)false |
dc.title.pt_BR.fl_str_mv |
Identification of suitable reference genes for real time quantitative polymerase chain reaction assays on pectoralis major muscle in chicken (Gallus gallus) |
title |
Identification of suitable reference genes for real time quantitative polymerase chain reaction assays on pectoralis major muscle in chicken (Gallus gallus) |
spellingShingle |
Identification of suitable reference genes for real time quantitative polymerase chain reaction assays on pectoralis major muscle in chicken (Gallus gallus) Nascimento, Carlos Souza do Aves Genes Gallus gallus Frangos Chicken |
title_short |
Identification of suitable reference genes for real time quantitative polymerase chain reaction assays on pectoralis major muscle in chicken (Gallus gallus) |
title_full |
Identification of suitable reference genes for real time quantitative polymerase chain reaction assays on pectoralis major muscle in chicken (Gallus gallus) |
title_fullStr |
Identification of suitable reference genes for real time quantitative polymerase chain reaction assays on pectoralis major muscle in chicken (Gallus gallus) |
title_full_unstemmed |
Identification of suitable reference genes for real time quantitative polymerase chain reaction assays on pectoralis major muscle in chicken (Gallus gallus) |
title_sort |
Identification of suitable reference genes for real time quantitative polymerase chain reaction assays on pectoralis major muscle in chicken (Gallus gallus) |
author |
Nascimento, Carlos Souza do |
author_facet |
Nascimento, Carlos Souza do Barbosa, Leandro Teixeira Brito, Claudson Fernandes, Roberta Pereira Miranda Silva-Mann, Renata Pinto, Ana Paula Gomes Oliveira, Haniel Cedraz de Dodson, Mike V. Guimarães, Simone Eliza Facioni Duarte, Marcio de Souza |
author_role |
author |
author2 |
Barbosa, Leandro Teixeira Brito, Claudson Fernandes, Roberta Pereira Miranda Silva-Mann, Renata Pinto, Ana Paula Gomes Oliveira, Haniel Cedraz de Dodson, Mike V. Guimarães, Simone Eliza Facioni Duarte, Marcio de Souza |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Nascimento, Carlos Souza do Barbosa, Leandro Teixeira Brito, Claudson Fernandes, Roberta Pereira Miranda Silva-Mann, Renata Pinto, Ana Paula Gomes Oliveira, Haniel Cedraz de Dodson, Mike V. Guimarães, Simone Eliza Facioni Duarte, Marcio de Souza |
dc.subject.por.fl_str_mv |
Aves Genes Gallus gallus Frangos |
topic |
Aves Genes Gallus gallus Frangos Chicken |
dc.subject.eng.fl_str_mv |
Chicken |
description |
Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPq |
publishDate |
2015 |
dc.date.issued.fl_str_mv |
2015-05 |
dc.date.accessioned.fl_str_mv |
2018-06-26T23:26:52Z |
dc.date.available.fl_str_mv |
2018-06-26T23:26:52Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
NASCIMENTO, C. S. et al. Identification of suitable reference genes for real time quantitative polymerase chain reaction assays on pectoralis major muscle in chicken (Gallus gallus). PLOS One, 28 maio 2015. Disponível em: <https://doi.org/10.1371/journal.pone.0127935>. Acesso em: 26 jun. 2018. |
dc.identifier.uri.fl_str_mv |
http://ri.ufs.br/jspui/handle/riufs/8493 |
dc.identifier.issn.none.fl_str_mv |
1932-6203 |
dc.identifier.license.pt_BR.fl_str_mv |
Creative Commons Atribuição 4.0 Internacional (CC BY 4.0) |
identifier_str_mv |
NASCIMENTO, C. S. et al. Identification of suitable reference genes for real time quantitative polymerase chain reaction assays on pectoralis major muscle in chicken (Gallus gallus). PLOS One, 28 maio 2015. Disponível em: <https://doi.org/10.1371/journal.pone.0127935>. Acesso em: 26 jun. 2018. 1932-6203 Creative Commons Atribuição 4.0 Internacional (CC BY 4.0) |
url |
http://ri.ufs.br/jspui/handle/riufs/8493 |
dc.language.iso.fl_str_mv |
por |
language |
por |
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PLOS One |
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info:eu-repo/semantics/openAccess |
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openAccess |
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PLOS |
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PLOS |
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Universidade Federal de Sergipe (UFS) |
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