Detection of specific IgE antibodies in parasite diseases
Autor(a) principal: | |
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Data de Publicação: | 1999 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFS |
Texto Completo: | https://ri.ufs.br/handle/riufs/928 |
Resumo: | Activation of Th1 or Th2 cells is associated with production of specific immunoglobulin isotypes, offering the opportunity to use antibody measurement for evaluation of T cell function. Schistosomiasis and visceral leishmaniasis are diseases associated with Th2 activation. However, an IgE response is not always detected in these patients. In the present study we evaluated specific IgE antibodies to S. mansoni and L. chagasi antigens by ELISA after depletion of serum IgG with protein G immobilized on Sepharose beads or RF-absorbent (purified sheep IgG antibodies anti-human IgG). In schistosomiasis patients, specific IgE to SWAP antigen was demonstrable in only 10 of 21 patients (48%) (mean absorbance ± SD = 0.102 ± 0.195) when unabsorbed serum was used. Depletion of IgG with protein G increased the number of specific IgE-positive tests to 13 (62%) and the use of RF-absorbent increased the number of positive results to 20 (95%) (mean absorbances ± SD = 0.303 ± 0.455 and 0.374 ± 0.477, respectively). Specific IgE anti-L. chagasi antibodies were not detected in unabsorbed serum from visceral leishmaniasis patients. When IgG was depleted with protein G, IgE antibodies were detected in only 3 (11%) of 27 patients, and the use of RF-absorbent permitted the detection of this isotype in all 27 visceral leishmaniasis sera tested (mean absorbance ± SD = 0.104 ± 0.03). These data show that the presence of IgG antibodies may prevent the detection of a specific IgE response in these parasite diseases. RF-absorbent, a reagent that blocks IgG-binding sites and also removes rheumatoid factor, was more efficient than protein G for the demonstration of specific IgE antibodies. |
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Atta, Maria Luiza Brito de SousaAraújo, Maria IlmaD'Oliveira Júnior, ArgemiroJesus, Amélia Maria Ribeiro deAlmeida, Roque Pacheco deAtta, Ajax MercêsCarvalho Filho, Edgar Marcelino de2014-02-21T20:03:22Z2014-02-21T20:03:22Z1999-09ATTA, M. L. B. S. et al. Detection of specific IgE antibodies in parasite diseases. Brazilian Journal of Medical and Biological Research, Ribeirão Preto, v. 32, n. 9, set. 1999. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000900008>. Acesso em: 21 fev. 2014.1414-431Xhttps://ri.ufs.br/handle/riufs/928Creative Commons Attribution LicenseActivation of Th1 or Th2 cells is associated with production of specific immunoglobulin isotypes, offering the opportunity to use antibody measurement for evaluation of T cell function. Schistosomiasis and visceral leishmaniasis are diseases associated with Th2 activation. However, an IgE response is not always detected in these patients. In the present study we evaluated specific IgE antibodies to S. mansoni and L. chagasi antigens by ELISA after depletion of serum IgG with protein G immobilized on Sepharose beads or RF-absorbent (purified sheep IgG antibodies anti-human IgG). In schistosomiasis patients, specific IgE to SWAP antigen was demonstrable in only 10 of 21 patients (48%) (mean absorbance ± SD = 0.102 ± 0.195) when unabsorbed serum was used. Depletion of IgG with protein G increased the number of specific IgE-positive tests to 13 (62%) and the use of RF-absorbent increased the number of positive results to 20 (95%) (mean absorbances ± SD = 0.303 ± 0.455 and 0.374 ± 0.477, respectively). Specific IgE anti-L. chagasi antibodies were not detected in unabsorbed serum from visceral leishmaniasis patients. When IgG was depleted with protein G, IgE antibodies were detected in only 3 (11%) of 27 patients, and the use of RF-absorbent permitted the detection of this isotype in all 27 visceral leishmaniasis sera tested (mean absorbance ± SD = 0.104 ± 0.03). These data show that the presence of IgG antibodies may prevent the detection of a specific IgE response in these parasite diseases. RF-absorbent, a reagent that blocks IgG-binding sites and also removes rheumatoid factor, was more efficient than protein G for the demonstration of specific IgE antibodies.Associação Brasileira de Divulgação CientíficaAnticorposImunoglobulina EEsquistossomoseParasitasDoenças parasitáriasLeishmaniose visceralCalazarDetection of specific IgE antibodies in parasite diseasesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleengreponame:Repositório Institucional da UFSinstname:Universidade Federal de Sergipe (UFS)instacron:UFSinfo:eu-repo/semantics/openAccessTHUMBNAILDetectionIgEantibodies.pdf.jpgDetectionIgEantibodies.pdf.jpgGenerated Thumbnailimage/jpeg1588https://ri.ufs.br/jspui/bitstream/riufs/928/4/DetectionIgEantibodies.pdf.jpgf672dd10d6efc1fd1390bdf0d000c1c5MD54ORIGINALDetectionIgEantibodies.pdfDetectionIgEantibodies.pdfapplication/pdf142712https://ri.ufs.br/jspui/bitstream/riufs/928/1/DetectionIgEantibodies.pdfbcd27ef26d17017807f392a7f6cbbec8MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://ri.ufs.br/jspui/bitstream/riufs/928/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52TEXTDetectionIgEantibodies.pdf.txtDetectionIgEantibodies.pdf.txtExtracted texttext/plain17795https://ri.ufs.br/jspui/bitstream/riufs/928/3/DetectionIgEantibodies.pdf.txtd83e781b2223861bf55d23088cd6700aMD53riufs/9282014-02-22 02:00:21.729oai:ufs.br:riufs/928Tk9URTogUExBQ0UgWU9VUiBPV04gTElDRU5TRSBIRVJFClRoaXMgc2FtcGxlIGxpY2Vuc2UgaXMgcHJvdmlkZWQgZm9yIGluZm9ybWF0aW9uYWwgcHVycG9zZXMgb25seS4KCk5PTi1FWENMVVNJVkUgRElTVFJJQlVUSU9OIExJQ0VOU0UKCkJ5IHNpZ25pbmcgYW5kIHN1Ym1pdHRpbmcgdGhpcyBsaWNlbnNlLCB5b3UgKHRoZSBhdXRob3Iocykgb3IgY29weXJpZ2h0Cm93bmVyKSBncmFudHMgdG8gRFNwYWNlIFVuaXZlcnNpdHkgKERTVSkgdGhlIG5vbi1leGNsdXNpdmUgcmlnaHQgdG8gcmVwcm9kdWNlLAp0cmFuc2xhdGUgKGFzIGRlZmluZWQgYmVsb3cpLCBhbmQvb3IgZGlzdHJpYnV0ZSB5b3VyIHN1Ym1pc3Npb24gKGluY2x1ZGluZwp0aGUgYWJzdHJhY3QpIHdvcmxkd2lkZSBpbiBwcmludCBhbmQgZWxlY3Ryb25pYyBmb3JtYXQgYW5kIGluIGFueSBtZWRpdW0sCmluY2x1ZGluZyBidXQgbm90IGxpbWl0ZWQgdG8gYXVkaW8gb3IgdmlkZW8uCgpZb3UgYWdyZWUgdGhhdCBEU1UgbWF5LCB3aXRob3V0IGNoYW5naW5nIHRoZSBjb250ZW50LCB0cmFuc2xhdGUgdGhlCnN1Ym1pc3Npb24gdG8gYW55IG1lZGl1bSBvciBmb3JtYXQgZm9yIHRoZSBwdXJwb3NlIG9mIHByZXNlcnZhdGlvbi4KCllvdSBhbHNvIGFncmVlIHRoYXQgRFNVIG1heSBrZWVwIG1vcmUgdGhhbiBvbmUgY29weSBvZiB0aGlzIHN1Ym1pc3Npb24gZm9yCnB1cnBvc2VzIG9mIHNlY3VyaXR5LCBiYWNrLXVwIGFuZCBwcmVzZXJ2YXRpb24uCgpZb3UgcmVwcmVzZW50IHRoYXQgdGhlIHN1Ym1pc3Npb24gaXMgeW91ciBvcmlnaW5hbCB3b3JrLCBhbmQgdGhhdCB5b3UgaGF2ZQp0aGUgcmlnaHQgdG8gZ3JhbnQgdGhlIHJpZ2h0cyBjb250YWluZWQgaW4gdGhpcyBsaWNlbnNlLiBZb3UgYWxzbyByZXByZXNlbnQKdGhhdCB5b3VyIHN1Ym1pc3Npb24gZG9lcyBub3QsIHRvIHRoZSBiZXN0IG9mIHlvdXIga25vd2xlZGdlLCBpbmZyaW5nZSB1cG9uCmFueW9uZSdzIGNvcHlyaWdodC4KCklmIHRoZSBzdWJtaXNzaW9uIGNvbnRhaW5zIG1hdGVyaWFsIGZvciB3aGljaCB5b3UgZG8gbm90IGhvbGQgY29weXJpZ2h0LAp5b3UgcmVwcmVzZW50IHRoYXQgeW91IGhhdmUgb2J0YWluZWQgdGhlIHVucmVzdHJpY3RlZCBwZXJtaXNzaW9uIG9mIHRoZQpjb3B5cmlnaHQgb3duZXIgdG8gZ3JhbnQgRFNVIHRoZSByaWdodHMgcmVxdWlyZWQgYnkgdGhpcyBsaWNlbnNlLCBhbmQgdGhhdApzdWNoIHRoaXJkLXBhcnR5IG93bmVkIG1hdGVyaWFsIGlzIGNsZWFybHkgaWRlbnRpZmllZCBhbmQgYWNrbm93bGVkZ2VkCndpdGhpbiB0aGUgdGV4dCBvciBjb250ZW50IG9mIHRoZSBzdWJtaXNzaW9uLgoKSUYgVEhFIFNVQk1JU1NJT04gSVMgQkFTRUQgVVBPTiBXT1JLIFRIQVQgSEFTIEJFRU4gU1BPTlNPUkVEIE9SIFNVUFBPUlRFRApCWSBBTiBBR0VOQ1kgT1IgT1JHQU5JWkFUSU9OIE9USEVSIFRIQU4gRFNVLCBZT1UgUkVQUkVTRU5UIFRIQVQgWU9VIEhBVkUKRlVMRklMTEVEIEFOWSBSSUdIVCBPRiBSRVZJRVcgT1IgT1RIRVIgT0JMSUdBVElPTlMgUkVRVUlSRUQgQlkgU1VDSApDT05UUkFDVCBPUiBBR1JFRU1FTlQuCgpEU1Ugd2lsbCBjbGVhcmx5IGlkZW50aWZ5IHlvdXIgbmFtZShzKSBhcyB0aGUgYXV0aG9yKHMpIG9yIG93bmVyKHMpIG9mIHRoZQpzdWJtaXNzaW9uLCBhbmQgd2lsbCBub3QgbWFrZSBhbnkgYWx0ZXJhdGlvbiwgb3RoZXIgdGhhbiBhcyBhbGxvd2VkIGJ5IHRoaXMKbGljZW5zZSwgdG8geW91ciBzdWJtaXNzaW9uLgo=Repositório InstitucionalPUBhttps://ri.ufs.br/oai/requestrepositorio@academico.ufs.bropendoar:2014-02-22T05:00:21Repositório Institucional da UFS - Universidade Federal de Sergipe (UFS)false |
dc.title.pt_BR.fl_str_mv |
Detection of specific IgE antibodies in parasite diseases |
title |
Detection of specific IgE antibodies in parasite diseases |
spellingShingle |
Detection of specific IgE antibodies in parasite diseases Atta, Maria Luiza Brito de Sousa Anticorpos Imunoglobulina E Esquistossomose Parasitas Doenças parasitárias Leishmaniose visceral Calazar |
title_short |
Detection of specific IgE antibodies in parasite diseases |
title_full |
Detection of specific IgE antibodies in parasite diseases |
title_fullStr |
Detection of specific IgE antibodies in parasite diseases |
title_full_unstemmed |
Detection of specific IgE antibodies in parasite diseases |
title_sort |
Detection of specific IgE antibodies in parasite diseases |
author |
Atta, Maria Luiza Brito de Sousa |
author_facet |
Atta, Maria Luiza Brito de Sousa Araújo, Maria Ilma D'Oliveira Júnior, Argemiro Jesus, Amélia Maria Ribeiro de Almeida, Roque Pacheco de Atta, Ajax Mercês Carvalho Filho, Edgar Marcelino de |
author_role |
author |
author2 |
Araújo, Maria Ilma D'Oliveira Júnior, Argemiro Jesus, Amélia Maria Ribeiro de Almeida, Roque Pacheco de Atta, Ajax Mercês Carvalho Filho, Edgar Marcelino de |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Atta, Maria Luiza Brito de Sousa Araújo, Maria Ilma D'Oliveira Júnior, Argemiro Jesus, Amélia Maria Ribeiro de Almeida, Roque Pacheco de Atta, Ajax Mercês Carvalho Filho, Edgar Marcelino de |
dc.subject.por.fl_str_mv |
Anticorpos Imunoglobulina E Esquistossomose Parasitas Doenças parasitárias Leishmaniose visceral Calazar |
topic |
Anticorpos Imunoglobulina E Esquistossomose Parasitas Doenças parasitárias Leishmaniose visceral Calazar |
description |
Activation of Th1 or Th2 cells is associated with production of specific immunoglobulin isotypes, offering the opportunity to use antibody measurement for evaluation of T cell function. Schistosomiasis and visceral leishmaniasis are diseases associated with Th2 activation. However, an IgE response is not always detected in these patients. In the present study we evaluated specific IgE antibodies to S. mansoni and L. chagasi antigens by ELISA after depletion of serum IgG with protein G immobilized on Sepharose beads or RF-absorbent (purified sheep IgG antibodies anti-human IgG). In schistosomiasis patients, specific IgE to SWAP antigen was demonstrable in only 10 of 21 patients (48%) (mean absorbance ± SD = 0.102 ± 0.195) when unabsorbed serum was used. Depletion of IgG with protein G increased the number of specific IgE-positive tests to 13 (62%) and the use of RF-absorbent increased the number of positive results to 20 (95%) (mean absorbances ± SD = 0.303 ± 0.455 and 0.374 ± 0.477, respectively). Specific IgE anti-L. chagasi antibodies were not detected in unabsorbed serum from visceral leishmaniasis patients. When IgG was depleted with protein G, IgE antibodies were detected in only 3 (11%) of 27 patients, and the use of RF-absorbent permitted the detection of this isotype in all 27 visceral leishmaniasis sera tested (mean absorbance ± SD = 0.104 ± 0.03). These data show that the presence of IgG antibodies may prevent the detection of a specific IgE response in these parasite diseases. RF-absorbent, a reagent that blocks IgG-binding sites and also removes rheumatoid factor, was more efficient than protein G for the demonstration of specific IgE antibodies. |
publishDate |
1999 |
dc.date.issued.fl_str_mv |
1999-09 |
dc.date.accessioned.fl_str_mv |
2014-02-21T20:03:22Z |
dc.date.available.fl_str_mv |
2014-02-21T20:03:22Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
ATTA, M. L. B. S. et al. Detection of specific IgE antibodies in parasite diseases. Brazilian Journal of Medical and Biological Research, Ribeirão Preto, v. 32, n. 9, set. 1999. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000900008>. Acesso em: 21 fev. 2014. |
dc.identifier.uri.fl_str_mv |
https://ri.ufs.br/handle/riufs/928 |
dc.identifier.issn.none.fl_str_mv |
1414-431X |
dc.identifier.license.pt_BR.fl_str_mv |
Creative Commons Attribution License |
identifier_str_mv |
ATTA, M. L. B. S. et al. Detection of specific IgE antibodies in parasite diseases. Brazilian Journal of Medical and Biological Research, Ribeirão Preto, v. 32, n. 9, set. 1999. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000900008>. Acesso em: 21 fev. 2014. 1414-431X Creative Commons Attribution License |
url |
https://ri.ufs.br/handle/riufs/928 |
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eng |
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eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
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Associação Brasileira de Divulgação Científica |
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Associação Brasileira de Divulgação Científica |
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reponame:Repositório Institucional da UFS instname:Universidade Federal de Sergipe (UFS) instacron:UFS |
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UFS |
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Repositório Institucional da UFS |
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