Aplicação de corantes alternativos em laminário histológico vegetal

• Autor(a) principal: Moura, Celine Silva
• Data de Publicação: 2022
• Tipo de documento: Trabalho de conclusão de curso
• Idioma: por
• Título da fonte: Repositório Institucional da UFS
• Texto Completo: http://ri.ufs.br/jspui/handle/riufs/16007
• topic: Biologia; Ensino de biologia; Anatomia vegetal; Corantes; Tecido vegetal; Plant anatomy; Coloring; Plant tissue; CIENCIAS BIOLOGICAS::BOTANICA::MORFOLOGIA VEGETAL::ANATOMIA VEGETAL

The use of dyes began in the 17th century, with the discovery of the scientist Antony van Leeuwenhoek in 1673. Natural dyes were used for many years, until in 1856, the English chemist William Henry Perkin, created the first dye. synthetic organic called Malvein. Since then, a revolution in the world of colorants began; natural ones were progressively replaced by synthetic ones, mainly in industries. Dyes are chemical compounds from a synthetic process or of natural origin, which adhere on a molecular scale to a substrate. Dyes have been used to dye fabrics for centuries, and powder dye is one of the affordable and low-cost options on the market. Thus, this work aimed to assemble a plant histological slide using low-cost textile dyes for tissue pigmentation, in order to expand the plant anatomical material in the plant anatomy laboratory (LAVD) collection to be used in a didactic way. The collection of botanical materials for this study was carried out in the municipality of São Cristóvão at two points: at the Federal University of Sergipe, Prof. José Aloísio de Campos; and banks of the Poxim River, close to UFS. Both the fresh material and the material fixed in 70% alcohol were handled to obtain the histological sections, following the freehand sectioning technique. To stain the samples, powder dyes from the brands Tupy and Guarany were used, which were diluted in a 50% hydroalcoholic solution and subsequently filtered. To stain the plant tissues, the histological sections were immersed in the dye in a closed petri dish, in an average time of 40-60min, then the sections were washed and placed in the fixative for 1min. Finally, the slides were mounted with glycerin gelatin produced with the following products: colorless gelatin, glycerin, 5 clove flower buds to replace phenol. As a result, it was possible to prove that the powder dye has the ability to pigment the plant tissues and enhance the visualization of the images, facilitating the observation of the tissues. Thus, it was possible to create a histological slide with 24 slides showing different parts of the plant such as: leaf, stem and root.