Microencapsulação de suco de laranja (Citrus sinensis) e colágeno hidrolisado por gelificação iônica

Detalhes bibliográficos
Autor(a) principal: Reis, Aline Andrade
Data de Publicação: 2021
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Institucional da UFS
Texto Completo: http://ri.ufs.br/jspui/handle/riufs/16190
Resumo: The growing concern of consumers with quality of life has driven the search for healthy foods and/or ingredients. One of these ingredients is collagen, and Vitamin C is essential for its synthesis as it acts in the hydrolyzing reaction of proline and lysine, amino acids responsible for the structure and function of collagen, contributing to the improvement of the skin's characteristics. Given the above, the aim of this study was to obtain microcapsules of orange juice and collagen hydrolyzed by ionic gelation, aiming at the elaboration of a product with conservation of bioactive compounds and amino acids. To obtain the microcapsules, two ionic gelling processes were performed using two different gelling agents: sodium alginate and agar agar. The microcapsules were prepared in two formulations: orange juice microcapsules with hydrolyzed collagen and sodium alginate gelling agent and orange juice microcapsules with hydrolyzed collagen and agar gelling agent. The microcapsules were stored at 5 ± 2°C for 16 days, analyzing color, TSS, pH, ATT, total proteins, glycine, hydroxyproline, proline, bioactive compounds and antioxidant capacity (DPPH, ABTS and FRAP). The analyzes were performed in triplicate with the data analyzed by ANOVA and the means compared using the Tukey test at a 5% significance level. During storage, the Vitamin C contents of SAL and SAG microcapsules showed significant divergence (p ≤ 0.05) in relation to time and treatments applied. The glycine contents of the SAL and SAG microcapsules statistically disagreed (p ≤ 0.05) with each other and in relation to time, with an increase in the contents until the end of storage. In the quantification of Hydroxyproline, the SAL and SAG samples showed a significant difference (p ≤ 0.05) between them, however in relation to time there was no significant divergence (p ≤ 0.05). In the Proline parameter, a significant difference was observed between treatments and times applied (p ≤ 0.05), and the SAG microcapsule presented much higher contents. With the data shown in this study, it can be concluded that the addition of hydrolyzed collagen and orange juice in the preparation of microcapsules enhances the protein, bioactive and antioxidant properties and enables the reduction in the use of synthetic antioxidants, making them an innovative product.
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spelling Reis, Aline AndradePagani, Alessandra Almeida Castro2022-08-23T22:15:06Z2022-08-23T22:15:06Z2021-05-31REIS, Aline Andrade. Microencapsulação de suco de laranja (Citrus sinensis) e colágeno hidrolisado por gelificação iônica. 2021. 100 f. Dissertação (Mestrado em Ciência e Tecnologia de Alimentos) – Universidade Federal de Sergipe, São Cristóvão, 2021.http://ri.ufs.br/jspui/handle/riufs/16190The growing concern of consumers with quality of life has driven the search for healthy foods and/or ingredients. One of these ingredients is collagen, and Vitamin C is essential for its synthesis as it acts in the hydrolyzing reaction of proline and lysine, amino acids responsible for the structure and function of collagen, contributing to the improvement of the skin's characteristics. Given the above, the aim of this study was to obtain microcapsules of orange juice and collagen hydrolyzed by ionic gelation, aiming at the elaboration of a product with conservation of bioactive compounds and amino acids. To obtain the microcapsules, two ionic gelling processes were performed using two different gelling agents: sodium alginate and agar agar. The microcapsules were prepared in two formulations: orange juice microcapsules with hydrolyzed collagen and sodium alginate gelling agent and orange juice microcapsules with hydrolyzed collagen and agar gelling agent. The microcapsules were stored at 5 ± 2°C for 16 days, analyzing color, TSS, pH, ATT, total proteins, glycine, hydroxyproline, proline, bioactive compounds and antioxidant capacity (DPPH, ABTS and FRAP). The analyzes were performed in triplicate with the data analyzed by ANOVA and the means compared using the Tukey test at a 5% significance level. During storage, the Vitamin C contents of SAL and SAG microcapsules showed significant divergence (p ≤ 0.05) in relation to time and treatments applied. The glycine contents of the SAL and SAG microcapsules statistically disagreed (p ≤ 0.05) with each other and in relation to time, with an increase in the contents until the end of storage. In the quantification of Hydroxyproline, the SAL and SAG samples showed a significant difference (p ≤ 0.05) between them, however in relation to time there was no significant divergence (p ≤ 0.05). In the Proline parameter, a significant difference was observed between treatments and times applied (p ≤ 0.05), and the SAG microcapsule presented much higher contents. With the data shown in this study, it can be concluded that the addition of hydrolyzed collagen and orange juice in the preparation of microcapsules enhances the protein, bioactive and antioxidant properties and enables the reduction in the use of synthetic antioxidants, making them an innovative product.A crescente preocupação do consumidor com a qualidade de vida tem impulsionado a busca por alimentos e/ou ingredientes saudáveis. Um desses ingredientes é o colágeno, e a Vitamina C é fundamental para a síntese do mesmo pois atua na reação de hidrolixação da prolina e da lisina, aminoácidos responsáveis pela estrutura e função do colágeno, contribuindo na melhoria das características da pele. Diante do exposto, o objetivo deste estudo foi a obtenção de microcápsulas de suco de laranja e colágeno hidrolisado por gelificação iônica, visando a elaboração de um produto com conservação de compostos bioativos e aminoácidos. Para a obtenção das microcápsulas, foram realizados dois processos de gelificação iônica utilizando dois gelificantes diferentes: alginato de sódio e ágar ágar. As microcápsulas foram elaboradas em duas formulações: microcápsulas de suco de laranja com colágeno hidrolisado e gelificante alginato de sódio e microcápsulas de suco de laranja com colágeno hidrolisado e gelificante ágar. As microcápsulas foram armazenadas a 5 ± 2°C por 16 dias, realizando as análises cor, SST, pH, ATT, proteínas totais, glicina, hidroxiprolina, prolina, compostos bioativos e capacidade antioxidante (DPPH, ABTS e FRAP). As análises foram realizadas em triplicata com os dados analisados por ANOVA e as médias comparadas utilizando-se o teste de Tukey ao nível de 5% de significância. No armazenamento, os teores de Vitamina C das microcápsulas SAL e SAG apresentaram divergência significativa (p ≤ 0,05) em relação ao tempo e aos tratamentos aplicados. Os teores de Glicina das microcápsulas SAL e SAG discordaram estatisticamente (p ≤ 0,05) entre si e em relação ao tempo, sendo constatado aumento nos teores até o final do armazenamento. Na quantificação de Hidroxiprolina, as amostras SAL e SAG apresentaram diferença significativa (p ≤ 0,05) entre si, no entanto em relação ao tempo não foi verificada divergência significativa (p ≤ 0,05). No parâmetro Prolina foi observada diferença significativa entre os tratamentos e tempos aplicados (p ≤ 0,05), sendo que a microcápsula SAG apresentou teores bem maiores. Com os dados mostrados neste estudo, pode-se concluir que a adição de colágeno hidrolisado e suco de laranja na elaboração de microcápsulas potencializa as propriedades protéicas, bioativas e antioxidantes e viabiliza a redução de uso de antioxidantes sintéticos, tornando-as um produto inovador.São CristóvãoporColágenoVitamina CAminoácidosColágeno hidrolisadoHydrolyzed collagenVitamin CAmino acidsCIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOSMicroencapsulação de suco de laranja (Citrus sinensis) e colágeno hidrolisado por gelificação iônicaMicroencapsulation of orange juice (Citrus sinensis) and collagen hydrolyzed by ionic gelationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisPós-Graduação em Ciência e Tecnologia de AlimentosUniversidade Federal de Sergipereponame:Repositório Institucional da UFSinstname:Universidade Federal de Sergipe (UFS)instacron:UFSinfo:eu-repo/semantics/openAccessLICENSElicense.txtlicense.txttext/plain; charset=utf-81475https://ri.ufs.br/jspui/bitstream/riufs/16190/1/license.txt098cbbf65c2c15e1fb2e49c5d306a44cMD51ORIGINALALINE_ANDRADE_REIS.pdfALINE_ANDRADE_REIS.pdfapplication/pdf2031377https://ri.ufs.br/jspui/bitstream/riufs/16190/2/ALINE_ANDRADE_REIS.pdf97b4ff47596b95f8899d208c540df30fMD52TEXTALINE_ANDRADE_REIS.pdf.txtALINE_ANDRADE_REIS.pdf.txtExtracted texttext/plain187118https://ri.ufs.br/jspui/bitstream/riufs/16190/3/ALINE_ANDRADE_REIS.pdf.txt5517aced0c83385e8a1f85a096139ed1MD53THUMBNAILALINE_ANDRADE_REIS.pdf.jpgALINE_ANDRADE_REIS.pdf.jpgGenerated Thumbnailimage/jpeg1291https://ri.ufs.br/jspui/bitstream/riufs/16190/4/ALINE_ANDRADE_REIS.pdf.jpg3e939180a5c0e8d291931d0981e165acMD54riufs/161902022-08-23 19:15:26.253oai:ufs.br:riufs/16190TElDRU7Dh0EgREUgRElTVFJJQlVJw4fDg08gTsODTy1FWENMVVNJVkEKCkNvbSBhIGFwcmVzZW50YcOnw6NvIGRlc3RhIGxpY2Vuw6dhLCB2b2PDqiAobyBhdXRvcihlcykgb3UgbyB0aXR1bGFyIGRvcyBkaXJlaXRvcyBkZSBhdXRvcikgY29uY2VkZSDDoCBVbml2ZXJzaWRhZGUgRmVkZXJhbCBkZSBTZXJnaXBlIG8gZGlyZWl0byBuw6NvLWV4Y2x1c2l2byBkZSByZXByb2R1emlyIHNldSB0cmFiYWxobyBubyBmb3JtYXRvIGVsZXRyw7RuaWNvLCBpbmNsdWluZG8gb3MgZm9ybWF0b3Mgw6F1ZGlvIG91IHbDrWRlby4KClZvY8OqIGNvbmNvcmRhIHF1ZSBhIFVuaXZlcnNpZGFkZSBGZWRlcmFsIGRlIFNlcmdpcGUgcG9kZSwgc2VtIGFsdGVyYXIgbyBjb250ZcO6ZG8sIHRyYW5zcG9yIHNldSB0cmFiYWxobyBwYXJhIHF1YWxxdWVyIG1laW8gb3UgZm9ybWF0byBwYXJhIGZpbnMgZGUgcHJlc2VydmHDp8Ojby4KClZvY8OqIHRhbWLDqW0gY29uY29yZGEgcXVlIGEgVW5pdmVyc2lkYWRlIEZlZGVyYWwgZGUgU2VyZ2lwZSBwb2RlIG1hbnRlciBtYWlzIGRlIHVtYSBjw7NwaWEgZGUgc2V1IHRyYWJhbGhvIHBhcmEgZmlucyBkZSBzZWd1cmFuw6dhLCBiYWNrLXVwIGUgcHJlc2VydmHDp8Ojby4KClZvY8OqIGRlY2xhcmEgcXVlIHNldSB0cmFiYWxobyDDqSBvcmlnaW5hbCBlIHF1ZSB2b2PDqiB0ZW0gbyBwb2RlciBkZSBjb25jZWRlciBvcyBkaXJlaXRvcyBjb250aWRvcyBuZXN0YSBsaWNlbsOnYS4gVm9jw6ogdGFtYsOpbSBkZWNsYXJhIHF1ZSBvIGRlcMOzc2l0bywgcXVlIHNlamEgZGUgc2V1IGNvbmhlY2ltZW50bywgbsOjbyBpbmZyaW5nZSBkaXJlaXRvcyBhdXRvcmFpcyBkZSBuaW5ndcOpbS4KCkNhc28gbyB0cmFiYWxobyBjb250ZW5oYSBtYXRlcmlhbCBxdWUgdm9jw6ogbsOjbyBwb3NzdWkgYSB0aXR1bGFyaWRhZGUgZG9zIGRpcmVpdG9zIGF1dG9yYWlzLCB2b2PDqiBkZWNsYXJhIHF1ZSBvYnRldmUgYSBwZXJtaXNzw6NvIGlycmVzdHJpdGEgZG8gZGV0ZW50b3IgZG9zIGRpcmVpdG9zIGF1dG9yYWlzIHBhcmEgY29uY2VkZXIgw6AgVW5pdmVyc2lkYWRlIEZlZGVyYWwgZGUgU2VyZ2lwZSBvcyBkaXJlaXRvcyBhcHJlc2VudGFkb3MgbmVzdGEgbGljZW7Dp2EsIGUgcXVlIGVzc2UgbWF0ZXJpYWwgZGUgcHJvcHJpZWRhZGUgZGUgdGVyY2Vpcm9zIGVzdMOhIGNsYXJhbWVudGUgaWRlbnRpZmljYWRvIGUgcmVjb25oZWNpZG8gbm8gdGV4dG8gb3Ugbm8gY29udGXDumRvLgoKQSBVbml2ZXJzaWRhZGUgRmVkZXJhbCBkZSBTZXJnaXBlIHNlIGNvbXByb21ldGUgYSBpZGVudGlmaWNhciBjbGFyYW1lbnRlIG8gc2V1IG5vbWUocykgb3UgbyhzKSBub21lKHMpIGRvKHMpIApkZXRlbnRvcihlcykgZG9zIGRpcmVpdG9zIGF1dG9yYWlzIGRvIHRyYWJhbGhvLCBlIG7Do28gZmFyw6EgcXVhbHF1ZXIgYWx0ZXJhw6fDo28sIGFsw6ltIGRhcXVlbGFzIGNvbmNlZGlkYXMgcG9yIGVzdGEgbGljZW7Dp2EuIAo=Repositório InstitucionalPUBhttps://ri.ufs.br/oai/requestrepositorio@academico.ufs.bropendoar:2022-08-23T22:15:26Repositório Institucional da UFS - Universidade Federal de Sergipe (UFS)false
dc.title.pt_BR.fl_str_mv Microencapsulação de suco de laranja (Citrus sinensis) e colágeno hidrolisado por gelificação iônica
dc.title.alternative.eng.fl_str_mv Microencapsulation of orange juice (Citrus sinensis) and collagen hydrolyzed by ionic gelation
title Microencapsulação de suco de laranja (Citrus sinensis) e colágeno hidrolisado por gelificação iônica
spellingShingle Microencapsulação de suco de laranja (Citrus sinensis) e colágeno hidrolisado por gelificação iônica
Reis, Aline Andrade
Colágeno
Vitamina C
Aminoácidos
Colágeno hidrolisado
Hydrolyzed collagen
Vitamin C
Amino acids
CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS
title_short Microencapsulação de suco de laranja (Citrus sinensis) e colágeno hidrolisado por gelificação iônica
title_full Microencapsulação de suco de laranja (Citrus sinensis) e colágeno hidrolisado por gelificação iônica
title_fullStr Microencapsulação de suco de laranja (Citrus sinensis) e colágeno hidrolisado por gelificação iônica
title_full_unstemmed Microencapsulação de suco de laranja (Citrus sinensis) e colágeno hidrolisado por gelificação iônica
title_sort Microencapsulação de suco de laranja (Citrus sinensis) e colágeno hidrolisado por gelificação iônica
author Reis, Aline Andrade
author_facet Reis, Aline Andrade
author_role author
dc.contributor.author.fl_str_mv Reis, Aline Andrade
dc.contributor.advisor1.fl_str_mv Pagani, Alessandra Almeida Castro
contributor_str_mv Pagani, Alessandra Almeida Castro
dc.subject.por.fl_str_mv Colágeno
Vitamina C
Aminoácidos
Colágeno hidrolisado
topic Colágeno
Vitamina C
Aminoácidos
Colágeno hidrolisado
Hydrolyzed collagen
Vitamin C
Amino acids
CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS
dc.subject.eng.fl_str_mv Hydrolyzed collagen
Vitamin C
Amino acids
dc.subject.cnpq.fl_str_mv CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS
description The growing concern of consumers with quality of life has driven the search for healthy foods and/or ingredients. One of these ingredients is collagen, and Vitamin C is essential for its synthesis as it acts in the hydrolyzing reaction of proline and lysine, amino acids responsible for the structure and function of collagen, contributing to the improvement of the skin's characteristics. Given the above, the aim of this study was to obtain microcapsules of orange juice and collagen hydrolyzed by ionic gelation, aiming at the elaboration of a product with conservation of bioactive compounds and amino acids. To obtain the microcapsules, two ionic gelling processes were performed using two different gelling agents: sodium alginate and agar agar. The microcapsules were prepared in two formulations: orange juice microcapsules with hydrolyzed collagen and sodium alginate gelling agent and orange juice microcapsules with hydrolyzed collagen and agar gelling agent. The microcapsules were stored at 5 ± 2°C for 16 days, analyzing color, TSS, pH, ATT, total proteins, glycine, hydroxyproline, proline, bioactive compounds and antioxidant capacity (DPPH, ABTS and FRAP). The analyzes were performed in triplicate with the data analyzed by ANOVA and the means compared using the Tukey test at a 5% significance level. During storage, the Vitamin C contents of SAL and SAG microcapsules showed significant divergence (p ≤ 0.05) in relation to time and treatments applied. The glycine contents of the SAL and SAG microcapsules statistically disagreed (p ≤ 0.05) with each other and in relation to time, with an increase in the contents until the end of storage. In the quantification of Hydroxyproline, the SAL and SAG samples showed a significant difference (p ≤ 0.05) between them, however in relation to time there was no significant divergence (p ≤ 0.05). In the Proline parameter, a significant difference was observed between treatments and times applied (p ≤ 0.05), and the SAG microcapsule presented much higher contents. With the data shown in this study, it can be concluded that the addition of hydrolyzed collagen and orange juice in the preparation of microcapsules enhances the protein, bioactive and antioxidant properties and enables the reduction in the use of synthetic antioxidants, making them an innovative product.
publishDate 2021
dc.date.issued.fl_str_mv 2021-05-31
dc.date.accessioned.fl_str_mv 2022-08-23T22:15:06Z
dc.date.available.fl_str_mv 2022-08-23T22:15:06Z
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dc.identifier.citation.fl_str_mv REIS, Aline Andrade. Microencapsulação de suco de laranja (Citrus sinensis) e colágeno hidrolisado por gelificação iônica. 2021. 100 f. Dissertação (Mestrado em Ciência e Tecnologia de Alimentos) – Universidade Federal de Sergipe, São Cristóvão, 2021.
dc.identifier.uri.fl_str_mv http://ri.ufs.br/jspui/handle/riufs/16190
identifier_str_mv REIS, Aline Andrade. Microencapsulação de suco de laranja (Citrus sinensis) e colágeno hidrolisado por gelificação iônica. 2021. 100 f. Dissertação (Mestrado em Ciência e Tecnologia de Alimentos) – Universidade Federal de Sergipe, São Cristóvão, 2021.
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