Assessment of genetic diversity of a native population of Eplingiella fruticosa: a plant with therapeutic potential
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFS |
Texto Completo: | http://ri.ufs.br/jspui/handle/riufs/7258 |
Resumo: | Eplingiella fruticosa (Salzm. ex Benth.) Harley & J.F.B. Pastore, ex Hyptis fruticosa Salzm. ex Benth. is an aromatic and medicinal plant of the family Lamiaceae, found mainly in regions with intense anthropic activity. Information on the genetic diversity of this species is scarce. However, it can be assessed using molecular markers that identify the level of diversity among phenotypically identical individuals. The present study aimed to characterize the genetic diversity of a native population of E. fruticosa from the State of Sergipe using ISSR molecular markers. Samples of 100 plants were collected in 11 municipalities of the State of Sergipe and analyzed using eight ISSR primers, resulting in 72 informative bands. The cluster analysis obtained using the neighbor joining method resulted in three groups: Group I consisted of 50 plants, mainly from the municipalities of Areia Branca, Estância, Japaratuba, Moita Bonita, Pirambu, and Salgado; Group II was formed by 21 plants, with nine representatives from the municipality of Itaporanga d’Ajuda and 13 representatives from other municipalities; Group III was composed by 29 plants, being represented mainly by the municipalities of Malhada dos Bois and São Cristóvão. The smallest genetic distance occurred between plants EPF94 and EPF96 (0.250), and the greatest distance occurred between plants EPF50 and EPF96 (0.9778). The Shannon index had a mean value of 0.42, and diversity was considered moderate. Heterozygosity had a mean value of 0.267 and was considered low. Polymorphic information content (0.253) was considered moderately informative. Genetic diversity of E. fruticosa plants was intermediate, and the results of the present study can assist in the conservation and use of the genetic resources of this species. |
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Silva, Dennis CrystianDiniz, Leandro Eugenio CardamoneBlank, Arie FitzgeraldNizio, Daniela Aparecida de CastroPinto, Jéssika Andreza OliveiraPereira, Katily Luize GarciaArrigoni-Blank, Maria de Fátima2018-01-29T21:54:30Z2018-01-29T21:54:30Z2017SILVA, D. C. et al. Assessment of genetic diversity of a native population of Eplingiella fruticosa: a plant with therapeutic potential. Genetics and Molecular Research, Ribeirão Preto, v. 16, n. 3, 2017. Disponível em: <https://www.geneticsmr.com/abstract/assessment-of-genetic-diversity-of-a-native-population-of-eplingiella-fruticosa-a-plant-with-therapeutic-potential-7385.html>. Acesso em: 29 jan. 2018.1676-5680http://ri.ufs.br/jspui/handle/riufs/7258Creative Commons Atribuição-Não Comercial-Compartilha Igual 4.0 Internacional (CC BY-NC-SA 4.0)engFundação de Pesquisas Científicas de Ribeirão Preto (FUNPEC-RP)Genetics and Molecular ResearchMedicinal plantsGenetic variabilityConservation programISSRPlantas medicinaisVariabilidade genéticaAssessment of genetic diversity of a native population of Eplingiella fruticosa: a plant with therapeutic potentialinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleEplingiella fruticosa (Salzm. ex Benth.) Harley & J.F.B. Pastore, ex Hyptis fruticosa Salzm. ex Benth. is an aromatic and medicinal plant of the family Lamiaceae, found mainly in regions with intense anthropic activity. Information on the genetic diversity of this species is scarce. However, it can be assessed using molecular markers that identify the level of diversity among phenotypically identical individuals. The present study aimed to characterize the genetic diversity of a native population of E. fruticosa from the State of Sergipe using ISSR molecular markers. Samples of 100 plants were collected in 11 municipalities of the State of Sergipe and analyzed using eight ISSR primers, resulting in 72 informative bands. The cluster analysis obtained using the neighbor joining method resulted in three groups: Group I consisted of 50 plants, mainly from the municipalities of Areia Branca, Estância, Japaratuba, Moita Bonita, Pirambu, and Salgado; Group II was formed by 21 plants, with nine representatives from the municipality of Itaporanga d’Ajuda and 13 representatives from other municipalities; Group III was composed by 29 plants, being represented mainly by the municipalities of Malhada dos Bois and São Cristóvão. The smallest genetic distance occurred between plants EPF94 and EPF96 (0.250), and the greatest distance occurred between plants EPF50 and EPF96 (0.9778). The Shannon index had a mean value of 0.42, and diversity was considered moderate. Heterozygosity had a mean value of 0.267 and was considered low. Polymorphic information content (0.253) was considered moderately informative. Genetic diversity of E. fruticosa plants was intermediate, and the results of the present study can assist in the conservation and use of the genetic resources of this species.reponame:Repositório Institucional da UFSinstname:Universidade Federal de Sergipe (UFS)instacron:UFSinfo:eu-repo/semantics/openAccessORIGINALAssessmentGeneticDiversityEplingiellaFruticosa.pdfAssessmentGeneticDiversityEplingiellaFruticosa.pdfapplication/pdf1947135https://ri.ufs.br/jspui/bitstream/riufs/7258/2/AssessmentGeneticDiversityEplingiellaFruticosa.pdfbefd627095a58c65ea8cdc4388282cdeMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81475https://ri.ufs.br/jspui/bitstream/riufs/7258/1/license.txt098cbbf65c2c15e1fb2e49c5d306a44cMD51TEXTAssessmentGeneticDiversityEplingiellaFruticosa.pdf.txtAssessmentGeneticDiversityEplingiellaFruticosa.pdf.txtExtracted texttext/plain30410https://ri.ufs.br/jspui/bitstream/riufs/7258/3/AssessmentGeneticDiversityEplingiellaFruticosa.pdf.txte6e71fb444ae54576fe015eb59e9c772MD53THUMBNAILAssessmentGeneticDiversityEplingiellaFruticosa.pdf.jpgAssessmentGeneticDiversityEplingiellaFruticosa.pdf.jpgGenerated Thumbnailimage/jpeg1484https://ri.ufs.br/jspui/bitstream/riufs/7258/4/AssessmentGeneticDiversityEplingiellaFruticosa.pdf.jpgbed8a26dcc35839ed92fe5890e15730bMD54riufs/72582018-01-29 18:54:30.29oai:ufs.br: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Repositório InstitucionalPUBhttps://ri.ufs.br/oai/requestrepositorio@academico.ufs.bropendoar:2018-01-29T21:54:30Repositório Institucional da UFS - Universidade Federal de Sergipe (UFS)false |
dc.title.pt_BR.fl_str_mv |
Assessment of genetic diversity of a native population of Eplingiella fruticosa: a plant with therapeutic potential |
title |
Assessment of genetic diversity of a native population of Eplingiella fruticosa: a plant with therapeutic potential |
spellingShingle |
Assessment of genetic diversity of a native population of Eplingiella fruticosa: a plant with therapeutic potential Silva, Dennis Crystian Medicinal plants Genetic variability Conservation program ISSR Plantas medicinais Variabilidade genética |
title_short |
Assessment of genetic diversity of a native population of Eplingiella fruticosa: a plant with therapeutic potential |
title_full |
Assessment of genetic diversity of a native population of Eplingiella fruticosa: a plant with therapeutic potential |
title_fullStr |
Assessment of genetic diversity of a native population of Eplingiella fruticosa: a plant with therapeutic potential |
title_full_unstemmed |
Assessment of genetic diversity of a native population of Eplingiella fruticosa: a plant with therapeutic potential |
title_sort |
Assessment of genetic diversity of a native population of Eplingiella fruticosa: a plant with therapeutic potential |
author |
Silva, Dennis Crystian |
author_facet |
Silva, Dennis Crystian Diniz, Leandro Eugenio Cardamone Blank, Arie Fitzgerald Nizio, Daniela Aparecida de Castro Pinto, Jéssika Andreza Oliveira Pereira, Katily Luize Garcia Arrigoni-Blank, Maria de Fátima |
author_role |
author |
author2 |
Diniz, Leandro Eugenio Cardamone Blank, Arie Fitzgerald Nizio, Daniela Aparecida de Castro Pinto, Jéssika Andreza Oliveira Pereira, Katily Luize Garcia Arrigoni-Blank, Maria de Fátima |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Silva, Dennis Crystian Diniz, Leandro Eugenio Cardamone Blank, Arie Fitzgerald Nizio, Daniela Aparecida de Castro Pinto, Jéssika Andreza Oliveira Pereira, Katily Luize Garcia Arrigoni-Blank, Maria de Fátima |
dc.subject.eng.fl_str_mv |
Medicinal plants Genetic variability Conservation program ISSR |
topic |
Medicinal plants Genetic variability Conservation program ISSR Plantas medicinais Variabilidade genética |
dc.subject.por.fl_str_mv |
Plantas medicinais Variabilidade genética |
description |
Eplingiella fruticosa (Salzm. ex Benth.) Harley & J.F.B. Pastore, ex Hyptis fruticosa Salzm. ex Benth. is an aromatic and medicinal plant of the family Lamiaceae, found mainly in regions with intense anthropic activity. Information on the genetic diversity of this species is scarce. However, it can be assessed using molecular markers that identify the level of diversity among phenotypically identical individuals. The present study aimed to characterize the genetic diversity of a native population of E. fruticosa from the State of Sergipe using ISSR molecular markers. Samples of 100 plants were collected in 11 municipalities of the State of Sergipe and analyzed using eight ISSR primers, resulting in 72 informative bands. The cluster analysis obtained using the neighbor joining method resulted in three groups: Group I consisted of 50 plants, mainly from the municipalities of Areia Branca, Estância, Japaratuba, Moita Bonita, Pirambu, and Salgado; Group II was formed by 21 plants, with nine representatives from the municipality of Itaporanga d’Ajuda and 13 representatives from other municipalities; Group III was composed by 29 plants, being represented mainly by the municipalities of Malhada dos Bois and São Cristóvão. The smallest genetic distance occurred between plants EPF94 and EPF96 (0.250), and the greatest distance occurred between plants EPF50 and EPF96 (0.9778). The Shannon index had a mean value of 0.42, and diversity was considered moderate. Heterozygosity had a mean value of 0.267 and was considered low. Polymorphic information content (0.253) was considered moderately informative. Genetic diversity of E. fruticosa plants was intermediate, and the results of the present study can assist in the conservation and use of the genetic resources of this species. |
publishDate |
2017 |
dc.date.issued.fl_str_mv |
2017 |
dc.date.accessioned.fl_str_mv |
2018-01-29T21:54:30Z |
dc.date.available.fl_str_mv |
2018-01-29T21:54:30Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
SILVA, D. C. et al. Assessment of genetic diversity of a native population of Eplingiella fruticosa: a plant with therapeutic potential. Genetics and Molecular Research, Ribeirão Preto, v. 16, n. 3, 2017. Disponível em: <https://www.geneticsmr.com/abstract/assessment-of-genetic-diversity-of-a-native-population-of-eplingiella-fruticosa-a-plant-with-therapeutic-potential-7385.html>. Acesso em: 29 jan. 2018. |
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http://ri.ufs.br/jspui/handle/riufs/7258 |
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1676-5680 |
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Creative Commons Atribuição-Não Comercial-Compartilha Igual 4.0 Internacional (CC BY-NC-SA 4.0) |
identifier_str_mv |
SILVA, D. C. et al. Assessment of genetic diversity of a native population of Eplingiella fruticosa: a plant with therapeutic potential. Genetics and Molecular Research, Ribeirão Preto, v. 16, n. 3, 2017. Disponível em: <https://www.geneticsmr.com/abstract/assessment-of-genetic-diversity-of-a-native-population-of-eplingiella-fruticosa-a-plant-with-therapeutic-potential-7385.html>. Acesso em: 29 jan. 2018. 1676-5680 Creative Commons Atribuição-Não Comercial-Compartilha Igual 4.0 Internacional (CC BY-NC-SA 4.0) |
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