Cytokinin Dehydrogenase activity in primary roots and characterization of primary metabolites from leaves and rootlets of Ricinus Communis
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFS |
Texto Completo: | https://ri.ufs.br/handle/riufs/1601 |
Resumo: | The aim of this study was to determine the activity of cytokinin dehydrogenase (CKX) and to measure other biochemical components in the primary leaves and radicles of castor seedlings (BRS Energia) in the initial phase of growth. The crude protein extract obtained after a 1-h extraction from the root tissues of seedlings showed no detectable CKX enzymatic activity when incubated with the substrate isopentenyl adenine for 1 h. However, after precipitation with ammonium sulfate at 70% saturation, the pellet showed CKX activity. The peroxidase enzyme activity was higher in the leaves than in the radicles. The total and reducing sugar content was 1.5 times higher in the leaves than in the radicles. The amino acid and protein contents were 6.4 and 9.2 times higher in the leaves than in the radicles, respectively. |
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Marques, Maria Elizabeth da CostaFreitas, Beatriz TupinambáGuedes, Tarciana OliveiraFernandes, Roberta Pereira Miranda2016-01-26T15:47:22Z2016-01-26T15:47:22Z2013-12MARQUES, M. E. C. et al. Cytokinin Dehydrogenase activity in primary roots and characterization of primary metabolites from leaves and rootlets of Ricinus Communis. Brazilian Archives of Biology and Technology, Curitiba, v. 56, n. 6, nov./dez. 2013. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000600003&lng=en&nrm=iso>. Acesso em: 26 jan. 2016.1516-8913https://ri.ufs.br/handle/riufs/1601Creative Commons Attribution LicenseThe aim of this study was to determine the activity of cytokinin dehydrogenase (CKX) and to measure other biochemical components in the primary leaves and radicles of castor seedlings (BRS Energia) in the initial phase of growth. The crude protein extract obtained after a 1-h extraction from the root tissues of seedlings showed no detectable CKX enzymatic activity when incubated with the substrate isopentenyl adenine for 1 h. However, after precipitation with ammonium sulfate at 70% saturation, the pellet showed CKX activity. The peroxidase enzyme activity was higher in the leaves than in the radicles. The total and reducing sugar content was 1.5 times higher in the leaves than in the radicles. The amino acid and protein contents were 6.4 and 9.2 times higher in the leaves than in the radicles, respectively.Instituto de Tecnologia do Paraná (Tecpar)Castor plantCytokinin dehydrogenaseBiochemical characterizationPeroxidaseMamonaCitoquinasBioquímicaCytokinin Dehydrogenase activity in primary roots and characterization of primary metabolites from leaves and rootlets of Ricinus Communisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleengreponame:Repositório Institucional da UFSinstname:Universidade Federal de Sergipe (UFS)instacron:UFSinfo:eu-repo/semantics/openAccessTHUMBNAILCytokininDehydrogenaseRicinus.pdf.jpgCytokininDehydrogenaseRicinus.pdf.jpgGenerated Thumbnailimage/jpeg1707https://ri.ufs.br/jspui/bitstream/riufs/1601/4/CytokininDehydrogenaseRicinus.pdf.jpgb461b70692b6a1eda014d458a702500eMD54ORIGINALCytokininDehydrogenaseRicinus.pdfCytokininDehydrogenaseRicinus.pdfapplication/pdf82218https://ri.ufs.br/jspui/bitstream/riufs/1601/1/CytokininDehydrogenaseRicinus.pdfdf2938cdfcfd4f80810029de685c75c2MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://ri.ufs.br/jspui/bitstream/riufs/1601/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52TEXTCytokininDehydrogenaseRicinus.pdf.txtCytokininDehydrogenaseRicinus.pdf.txtExtracted texttext/plain30411https://ri.ufs.br/jspui/bitstream/riufs/1601/3/CytokininDehydrogenaseRicinus.pdf.txte3bc590607ed0f1e6aeda67e0c5e1e8fMD53riufs/16012016-01-28 21:02:22.878oai:ufs.br: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Repositório InstitucionalPUBhttps://ri.ufs.br/oai/requestrepositorio@academico.ufs.bropendoar:2016-01-29T00:02:22Repositório Institucional da UFS - Universidade Federal de Sergipe (UFS)false |
dc.title.pt_BR.fl_str_mv |
Cytokinin Dehydrogenase activity in primary roots and characterization of primary metabolites from leaves and rootlets of Ricinus Communis |
title |
Cytokinin Dehydrogenase activity in primary roots and characterization of primary metabolites from leaves and rootlets of Ricinus Communis |
spellingShingle |
Cytokinin Dehydrogenase activity in primary roots and characterization of primary metabolites from leaves and rootlets of Ricinus Communis Marques, Maria Elizabeth da Costa Castor plant Cytokinin dehydrogenase Biochemical characterization Peroxidase Mamona Citoquinas Bioquímica |
title_short |
Cytokinin Dehydrogenase activity in primary roots and characterization of primary metabolites from leaves and rootlets of Ricinus Communis |
title_full |
Cytokinin Dehydrogenase activity in primary roots and characterization of primary metabolites from leaves and rootlets of Ricinus Communis |
title_fullStr |
Cytokinin Dehydrogenase activity in primary roots and characterization of primary metabolites from leaves and rootlets of Ricinus Communis |
title_full_unstemmed |
Cytokinin Dehydrogenase activity in primary roots and characterization of primary metabolites from leaves and rootlets of Ricinus Communis |
title_sort |
Cytokinin Dehydrogenase activity in primary roots and characterization of primary metabolites from leaves and rootlets of Ricinus Communis |
author |
Marques, Maria Elizabeth da Costa |
author_facet |
Marques, Maria Elizabeth da Costa Freitas, Beatriz Tupinambá Guedes, Tarciana Oliveira Fernandes, Roberta Pereira Miranda |
author_role |
author |
author2 |
Freitas, Beatriz Tupinambá Guedes, Tarciana Oliveira Fernandes, Roberta Pereira Miranda |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Marques, Maria Elizabeth da Costa Freitas, Beatriz Tupinambá Guedes, Tarciana Oliveira Fernandes, Roberta Pereira Miranda |
dc.subject.por.fl_str_mv |
Castor plant Cytokinin dehydrogenase Biochemical characterization Peroxidase Mamona Citoquinas Bioquímica |
topic |
Castor plant Cytokinin dehydrogenase Biochemical characterization Peroxidase Mamona Citoquinas Bioquímica |
description |
The aim of this study was to determine the activity of cytokinin dehydrogenase (CKX) and to measure other biochemical components in the primary leaves and radicles of castor seedlings (BRS Energia) in the initial phase of growth. The crude protein extract obtained after a 1-h extraction from the root tissues of seedlings showed no detectable CKX enzymatic activity when incubated with the substrate isopentenyl adenine for 1 h. However, after precipitation with ammonium sulfate at 70% saturation, the pellet showed CKX activity. The peroxidase enzyme activity was higher in the leaves than in the radicles. The total and reducing sugar content was 1.5 times higher in the leaves than in the radicles. The amino acid and protein contents were 6.4 and 9.2 times higher in the leaves than in the radicles, respectively. |
publishDate |
2013 |
dc.date.issued.fl_str_mv |
2013-12 |
dc.date.accessioned.fl_str_mv |
2016-01-26T15:47:22Z |
dc.date.available.fl_str_mv |
2016-01-26T15:47:22Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
MARQUES, M. E. C. et al. Cytokinin Dehydrogenase activity in primary roots and characterization of primary metabolites from leaves and rootlets of Ricinus Communis. Brazilian Archives of Biology and Technology, Curitiba, v. 56, n. 6, nov./dez. 2013. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000600003&lng=en&nrm=iso>. Acesso em: 26 jan. 2016. |
dc.identifier.uri.fl_str_mv |
https://ri.ufs.br/handle/riufs/1601 |
dc.identifier.issn.none.fl_str_mv |
1516-8913 |
dc.identifier.license.pt_BR.fl_str_mv |
Creative Commons Attribution License |
identifier_str_mv |
MARQUES, M. E. C. et al. Cytokinin Dehydrogenase activity in primary roots and characterization of primary metabolites from leaves and rootlets of Ricinus Communis. Brazilian Archives of Biology and Technology, Curitiba, v. 56, n. 6, nov./dez. 2013. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000600003&lng=en&nrm=iso>. Acesso em: 26 jan. 2016. 1516-8913 Creative Commons Attribution License |
url |
https://ri.ufs.br/handle/riufs/1601 |
dc.language.iso.fl_str_mv |
eng |
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eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
dc.publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná (Tecpar) |
publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná (Tecpar) |
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UFS |
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