Extração assistida por ultrassom e micro-ondas dos compostos bioativos do bagaço e cascas de manga (Mangifera indica) : caracterização química e avaliação antioxidante

Detalhes bibliográficos
Autor(a) principal: Oliveira, Julio Manoel Andrade
Data de Publicação: 2023
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Institucional da UFS
Texto Completo: https://ri.ufs.br/jspui/handle/riufs/18830
Resumo: Mango (Mangifera indica) is a fruit worldwide consumed in natura, in juices, jellies and ice cream. However, its processing generates a significant amount of waste, considered an environmental problem. This work aimed to study the reuse of industrial mango waste (bagasse and peel) through the extraction of its bioactive components using extraction techniques with environmentally friendly solvents, as a way of directing this material to other more useful purposes. Ultrasoundassisted extraction (UAE) and Microwave-assisted extraction (MAE) were applied in order to obtain extracts with higher biological activities and yields, being directly compared with maceration extraction. Identification of bioactive compounds was performed using high-resolution mass spectrometry, followed by antioxidant activities. The optimization by EAU was carried out using the response surface methodology (RSM), which resulted in the optimal extraction condition using an ethanol concentration of 58.28%, solvent to solute ratio of 7.929 mL to 0.2 g, time of 5 min, frequency of 37 kHz, temperature of 40 ºC and 30% power. The optimal EAM condition, obtained by RSM, was ethanol concentration of 48.981%, time of 11 minutes and 6 s, solvent to solute ratio of 16 mL to 0.2 g, temperature of 84 ºC and power of 500W. Under these conditions, the yield obtained for the mango bagasse extract by UAE was 38.07% and the total phenolic content (TPC) was 101.63 mg EAG/g dw, values greater than those for the peel extracts of mango (36.07% and 92.95 mg EAG/g dw). The MAE extract from the bagasse resulted in a yield of 48.01% and TPC of 124.96 mg EAG/g dw, and for the peels they were 38.86% and 102.16 mg EAG/g dw, respectively. The extracts obtained by MAE showed higher values of yields, TPC and TFC compared to the UAE extracts. Antioxidant assays demonstrated that MAE extracts showed greater inhibition potentials compared to UAE extracts. In this work it was possible, by using Mass Spectrometry, to propose 39 chemical markers in the bagasse extracts and 28 for the peels extracts.
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spelling Oliveira, Julio Manoel AndradeMoraes, Valéria Regina de Souza2023-12-21T17:07:03Z2023-12-21T17:07:03Z2023-06-28OLIVEIRA, Julio Manoel Andrade. Extração assistida por ultrassom e micro-ondas dos compostos bioativos do bagaço e cascas de manga (Mangifera indica) : caracterização química e avaliação antioxidante. 2023. 152 f. Dissertação (Mestrado em Química) - Universidade Federal de Sergipe, São Cristóvão, 2023.https://ri.ufs.br/jspui/handle/riufs/18830Mango (Mangifera indica) is a fruit worldwide consumed in natura, in juices, jellies and ice cream. However, its processing generates a significant amount of waste, considered an environmental problem. This work aimed to study the reuse of industrial mango waste (bagasse and peel) through the extraction of its bioactive components using extraction techniques with environmentally friendly solvents, as a way of directing this material to other more useful purposes. Ultrasoundassisted extraction (UAE) and Microwave-assisted extraction (MAE) were applied in order to obtain extracts with higher biological activities and yields, being directly compared with maceration extraction. Identification of bioactive compounds was performed using high-resolution mass spectrometry, followed by antioxidant activities. The optimization by EAU was carried out using the response surface methodology (RSM), which resulted in the optimal extraction condition using an ethanol concentration of 58.28%, solvent to solute ratio of 7.929 mL to 0.2 g, time of 5 min, frequency of 37 kHz, temperature of 40 ºC and 30% power. The optimal EAM condition, obtained by RSM, was ethanol concentration of 48.981%, time of 11 minutes and 6 s, solvent to solute ratio of 16 mL to 0.2 g, temperature of 84 ºC and power of 500W. Under these conditions, the yield obtained for the mango bagasse extract by UAE was 38.07% and the total phenolic content (TPC) was 101.63 mg EAG/g dw, values greater than those for the peel extracts of mango (36.07% and 92.95 mg EAG/g dw). The MAE extract from the bagasse resulted in a yield of 48.01% and TPC of 124.96 mg EAG/g dw, and for the peels they were 38.86% and 102.16 mg EAG/g dw, respectively. The extracts obtained by MAE showed higher values of yields, TPC and TFC compared to the UAE extracts. Antioxidant assays demonstrated that MAE extracts showed greater inhibition potentials compared to UAE extracts. In this work it was possible, by using Mass Spectrometry, to propose 39 chemical markers in the bagasse extracts and 28 for the peels extracts.A manga (Mangifera indica) é uma fruta mundialmente consumida na forma in natura, em sucos, geleias e sorvetes, entretanto, esse processamento gera uma quantidade significativa de resíduos, considerado um problema ambiental. Este trabalho teve como objetivo o estudo do reaproveitamento dos resíduos industriais da manga (bagaço e cascas) através das extrações de seus componentes bioativos empregando técnicas de extrações com solventes ambientalmente amigáveis, como uma forma de direcionarmos este material para outras finalidades mais úteis. As extrações assistidas por ultrassom (EAU) e por micro-ondas (EAM) foram aplicadas com o objetivo de obter extratos com maiores conteúdos de fenólicos totais e rendimentos, sendo diretamente comparadas com a extração por maceração. A otimização por EAU foi realizada a partir da utilização da metodologia de superfície de resposta (RSM), onde resultou na condição ótima de extração utilizando a concentração de etanol de 58,280%, relação solvente para soluto de 7,929 mL para 0,2 g, tempo de 5 min, frequência de 37 kHz, temperatura de 40 ºC e 30% de potência. A condição ótima da EAM, obtida por RSM, foi concentração de etanol de 48,980%, tempo de 11 minutos e 6 s, relação solvente para soluto de 16 mL para 0,2 g, com temperatura de 84 ºC e potência de 500W. Nestas condições, o rendimento obtido para o extrato do bagaço da manga por EAU foi de 38,07% e o conteúdo de fenólicos totais (TPC) foi de 101,63 mg EAG/g ms (miligrama de Equivalente de Ácido Gálico por grama de material seco), valores maiores do que para os extratos das cascas de manga (36,07% e 92,95 mg EAG/g ms). O extrato EAM do bagaço resultou no rendimento de 48,01% e TPC de 124,96 mg EAG/g ms, e para as cascas foram de 38,86% e 102,16 mg EAG/g ms, respectivamente. Os extratos obtidos por EAM apresentam maiores valores de rendimentos, TPC e TFC em comparação com os extratos EAU. Os ensaios antioxidantes demonstraram que os extratos obtidos por EAM apresentaram maiores potenciais de inibição em relação aos de EAU. Através da Espectrometria de Massas foi possível propor 39 marcadores químicos nos extratos do bagaço e 26 para as cascas.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESSão CristóvãoporMangaExtração (química)Espectrometria de massaExtração assistida por ultrassomExtração assistida por micro-ondasAtividades antioxidantesMangoUltrasound assisted extractionMicrowave assisted extractionMass spectrometryAntioxidant activitiesCIENCIAS EXATAS E DA TERRA::QUIMICAExtração assistida por ultrassom e micro-ondas dos compostos bioativos do bagaço e cascas de manga (Mangifera indica) : caracterização química e avaliação antioxidanteUltrasound and microwave assisted extraction of bioactive compounds from mango bagasse and peels (Mangifera indica) : chemical characterization and antioxidant evaluationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisPós-Graduação em QuímicaUniversidade Federal de Sergipe (UFS)reponame:Repositório Institucional da UFSinstname:Universidade Federal de Sergipe (UFS)instacron:UFSinfo:eu-repo/semantics/openAccessLICENSElicense.txtlicense.txttext/plain; charset=utf-81475https://ri.ufs.br/jspui/bitstream/riufs/18830/1/license.txt098cbbf65c2c15e1fb2e49c5d306a44cMD51ORIGINALJULIO_MANOEL_ANDRADE_OLIVEIRA.pdfJULIO_MANOEL_ANDRADE_OLIVEIRA.pdfapplication/pdf2704612https://ri.ufs.br/jspui/bitstream/riufs/18830/2/JULIO_MANOEL_ANDRADE_OLIVEIRA.pdfc461d79c998089a80fbe307b02478808MD52riufs/188302023-12-21 14:07:09.139oai:ufs.br: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Repositório InstitucionalPUBhttps://ri.ufs.br/oai/requestrepositorio@academico.ufs.bropendoar:2023-12-21T17:07:09Repositório Institucional da UFS - Universidade Federal de Sergipe (UFS)false
dc.title.pt_BR.fl_str_mv Extração assistida por ultrassom e micro-ondas dos compostos bioativos do bagaço e cascas de manga (Mangifera indica) : caracterização química e avaliação antioxidante
dc.title.alternative.eng.fl_str_mv Ultrasound and microwave assisted extraction of bioactive compounds from mango bagasse and peels (Mangifera indica) : chemical characterization and antioxidant evaluation
title Extração assistida por ultrassom e micro-ondas dos compostos bioativos do bagaço e cascas de manga (Mangifera indica) : caracterização química e avaliação antioxidante
spellingShingle Extração assistida por ultrassom e micro-ondas dos compostos bioativos do bagaço e cascas de manga (Mangifera indica) : caracterização química e avaliação antioxidante
Oliveira, Julio Manoel Andrade
Manga
Extração (química)
Espectrometria de massa
Extração assistida por ultrassom
Extração assistida por micro-ondas
Atividades antioxidantes
Mango
Ultrasound assisted extraction
Microwave assisted extraction
Mass spectrometry
Antioxidant activities
CIENCIAS EXATAS E DA TERRA::QUIMICA
title_short Extração assistida por ultrassom e micro-ondas dos compostos bioativos do bagaço e cascas de manga (Mangifera indica) : caracterização química e avaliação antioxidante
title_full Extração assistida por ultrassom e micro-ondas dos compostos bioativos do bagaço e cascas de manga (Mangifera indica) : caracterização química e avaliação antioxidante
title_fullStr Extração assistida por ultrassom e micro-ondas dos compostos bioativos do bagaço e cascas de manga (Mangifera indica) : caracterização química e avaliação antioxidante
title_full_unstemmed Extração assistida por ultrassom e micro-ondas dos compostos bioativos do bagaço e cascas de manga (Mangifera indica) : caracterização química e avaliação antioxidante
title_sort Extração assistida por ultrassom e micro-ondas dos compostos bioativos do bagaço e cascas de manga (Mangifera indica) : caracterização química e avaliação antioxidante
author Oliveira, Julio Manoel Andrade
author_facet Oliveira, Julio Manoel Andrade
author_role author
dc.contributor.author.fl_str_mv Oliveira, Julio Manoel Andrade
dc.contributor.advisor1.fl_str_mv Moraes, Valéria Regina de Souza
contributor_str_mv Moraes, Valéria Regina de Souza
dc.subject.por.fl_str_mv Manga
Extração (química)
Espectrometria de massa
Extração assistida por ultrassom
Extração assistida por micro-ondas
Atividades antioxidantes
topic Manga
Extração (química)
Espectrometria de massa
Extração assistida por ultrassom
Extração assistida por micro-ondas
Atividades antioxidantes
Mango
Ultrasound assisted extraction
Microwave assisted extraction
Mass spectrometry
Antioxidant activities
CIENCIAS EXATAS E DA TERRA::QUIMICA
dc.subject.eng.fl_str_mv Mango
Ultrasound assisted extraction
Microwave assisted extraction
Mass spectrometry
Antioxidant activities
dc.subject.cnpq.fl_str_mv CIENCIAS EXATAS E DA TERRA::QUIMICA
description Mango (Mangifera indica) is a fruit worldwide consumed in natura, in juices, jellies and ice cream. However, its processing generates a significant amount of waste, considered an environmental problem. This work aimed to study the reuse of industrial mango waste (bagasse and peel) through the extraction of its bioactive components using extraction techniques with environmentally friendly solvents, as a way of directing this material to other more useful purposes. Ultrasoundassisted extraction (UAE) and Microwave-assisted extraction (MAE) were applied in order to obtain extracts with higher biological activities and yields, being directly compared with maceration extraction. Identification of bioactive compounds was performed using high-resolution mass spectrometry, followed by antioxidant activities. The optimization by EAU was carried out using the response surface methodology (RSM), which resulted in the optimal extraction condition using an ethanol concentration of 58.28%, solvent to solute ratio of 7.929 mL to 0.2 g, time of 5 min, frequency of 37 kHz, temperature of 40 ºC and 30% power. The optimal EAM condition, obtained by RSM, was ethanol concentration of 48.981%, time of 11 minutes and 6 s, solvent to solute ratio of 16 mL to 0.2 g, temperature of 84 ºC and power of 500W. Under these conditions, the yield obtained for the mango bagasse extract by UAE was 38.07% and the total phenolic content (TPC) was 101.63 mg EAG/g dw, values greater than those for the peel extracts of mango (36.07% and 92.95 mg EAG/g dw). The MAE extract from the bagasse resulted in a yield of 48.01% and TPC of 124.96 mg EAG/g dw, and for the peels they were 38.86% and 102.16 mg EAG/g dw, respectively. The extracts obtained by MAE showed higher values of yields, TPC and TFC compared to the UAE extracts. Antioxidant assays demonstrated that MAE extracts showed greater inhibition potentials compared to UAE extracts. In this work it was possible, by using Mass Spectrometry, to propose 39 chemical markers in the bagasse extracts and 28 for the peels extracts.
publishDate 2023
dc.date.accessioned.fl_str_mv 2023-12-21T17:07:03Z
dc.date.available.fl_str_mv 2023-12-21T17:07:03Z
dc.date.issued.fl_str_mv 2023-06-28
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
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dc.identifier.citation.fl_str_mv OLIVEIRA, Julio Manoel Andrade. Extração assistida por ultrassom e micro-ondas dos compostos bioativos do bagaço e cascas de manga (Mangifera indica) : caracterização química e avaliação antioxidante. 2023. 152 f. Dissertação (Mestrado em Química) - Universidade Federal de Sergipe, São Cristóvão, 2023.
dc.identifier.uri.fl_str_mv https://ri.ufs.br/jspui/handle/riufs/18830
identifier_str_mv OLIVEIRA, Julio Manoel Andrade. Extração assistida por ultrassom e micro-ondas dos compostos bioativos do bagaço e cascas de manga (Mangifera indica) : caracterização química e avaliação antioxidante. 2023. 152 f. Dissertação (Mestrado em Química) - Universidade Federal de Sergipe, São Cristóvão, 2023.
url https://ri.ufs.br/jspui/handle/riufs/18830
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dc.publisher.initials.fl_str_mv Universidade Federal de Sergipe (UFS)
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