Caracterização por espectrometria de massas dos flavonoides extraídos por técnicas assistidas por ultrassom e micro-ondas das folhas de Croton grewioides Baill.

Detalhes bibliográficos
Autor(a) principal: Nascimento, Pedro Ellison Santos do
Data de Publicação: 2021
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Institucional da UFS
Texto Completo: https://ri.ufs.br/jspui/handle/riufs/14905
Resumo: The complexity of compounds present in plants allows them to be used for different diseases, which is due to the presence of bioactive compounds named as secondary metabolites. Considering the lack of chemical knowledge of Croton grewioides species and the need to establish new methodologies for the extraction of its antioxidante compounds, in this work was carried out the optimization of the extraction processes by ultrasound and microwave using an experimental planning, aided by statistical analysis. Thus, it was possible to determine the best extraction conditions for each of the technique studied, ultrasound-assisted extraction (UAE) and microwave assisted extraction (MAE). From the best conditions of extraction, the extracts of C. grewioides were analyzed by liquid chromatography and their fingerprint chromatograms submitted to chemometric analysis. High resolution mass spectrometry analyzes were also carried out, using the Orbitrap analyzer, with the objective of obtaining a chemical profile of mass spectrums, chemical profile of the extracts, and try to chemically identify the molecular formula and propose the chemical structure to the largest number possible of compounds present in this species. Through the chemometric results from both fingerprint, the identified metabolites and the antioxidant activities obtained, it was possible to assigned that the best extraction technique for antioxidant compounds from C. grewioides is microwave assisted extraction. Additionally, it was possible to propose, for the first time, structures of forty one compounds in the extracts of this species from the analysis of high resolution mass spectrometry, being considered an unprecedented method for the chemical study of this species.
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spelling Nascimento, Pedro Ellison Santos doMoraes, Valéria Regina de SouzaPrado, Vilma Menezes de Jesus2021-12-16T18:57:11Z2021-12-16T18:57:11Z2021-01-29NASCIMENTO, Pedro Ellison Santos do. Caracterização por espectrometria de massas dos flavonoides extraídos por técnicas assistidas por ultrassom e micro-ondas das folhas de Croton grewioides Baill. 2021. 130 f. Dissertação (Mestrado em Química) – Universidade Federal de Sergipe, São Cristóvão, 2021.https://ri.ufs.br/jspui/handle/riufs/14905The complexity of compounds present in plants allows them to be used for different diseases, which is due to the presence of bioactive compounds named as secondary metabolites. Considering the lack of chemical knowledge of Croton grewioides species and the need to establish new methodologies for the extraction of its antioxidante compounds, in this work was carried out the optimization of the extraction processes by ultrasound and microwave using an experimental planning, aided by statistical analysis. Thus, it was possible to determine the best extraction conditions for each of the technique studied, ultrasound-assisted extraction (UAE) and microwave assisted extraction (MAE). From the best conditions of extraction, the extracts of C. grewioides were analyzed by liquid chromatography and their fingerprint chromatograms submitted to chemometric analysis. High resolution mass spectrometry analyzes were also carried out, using the Orbitrap analyzer, with the objective of obtaining a chemical profile of mass spectrums, chemical profile of the extracts, and try to chemically identify the molecular formula and propose the chemical structure to the largest number possible of compounds present in this species. Through the chemometric results from both fingerprint, the identified metabolites and the antioxidant activities obtained, it was possible to assigned that the best extraction technique for antioxidant compounds from C. grewioides is microwave assisted extraction. Additionally, it was possible to propose, for the first time, structures of forty one compounds in the extracts of this species from the analysis of high resolution mass spectrometry, being considered an unprecedented method for the chemical study of this species.A complexidade de compostos presentes nas plantas permite que elas possam ser utilizadas em diferentes enfermidades, o qual só é possível devido a presença de compostos bioativos que constituem seus metabólitos secundários. Considerando a escassez no conhecimento químico da espécie Croton grewioides e a necessidade de se estabelecer novas metodologias de extração de seus compostos antioxidantes, neste trabalho foi realizado a otimização dos processos de extração por ultrassom e micro-ondas utilizando um planejamento experimental, auxiliados por tratamentos estatísticos. Foi possível a determinação das melhores condições de extração para cada uma das técnicas estudadas, a extração assistida por ultrassom (EAU) e a extração assistida por micro-ondas (EAM). Determinadas as melhores condições de extração, os extratos de C. grewioides foram analisados por cromatografia líquida e seus cromatogramas fingerprint submetidos a análise quimiométrica. Também foram feitas análises por espectrometria de massas de alta resolução, utilizando o analisador Orbitrap, com o objetivo de se obter os espectros de massas perfil químico dos extratos, atribuir a fórmula molecular e propor a estrutura química ao maior número possível de compostos presentes nesta espécie. Através dos resultados quimiométricos dos dois fingerprints, dos metabólitos identificados e dos dados de atividades antioxidantes obtidos, foi possível atribuir que a melhor técnica de extração para compostos antioxidantes a partir de C. grewioides é a extração assistida por micro-ondas. Adicionalmente, foi possível propor, pela primeira vez, estruturas de quarenta e um compostos nos extratos desta espécie a partir da análise de espectrometria de massas de alta resolução, sendo considerado um método inédito para o estudo químico desta espécie.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESSão CristóvãoporQuímicaUltrassomMicro-ondasAntioxidantesCroton grewioidesPlanejamento experimentalExtração assistida por ultrassomExtração assistida por micro-ondasEspectrometria de massas de alta resoluçãoExperimental designUltrasound-assisted extractionMicrowave-assisted-extractionHigh resolution mass spectrometryAntioxidantsCIENCIAS EXATAS E DA TERRA::QUIMICACaracterização por espectrometria de massas dos flavonoides extraídos por técnicas assistidas por ultrassom e micro-ondas das folhas de Croton grewioides Baill.Mass spectrometry in the characterization of flavonoids extracted by using microwave and ultrasound-assisted of leaves from Croton grewioides Baill.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisPós-Graduação em QuímicaUniversidade Federal de Sergipereponame:Repositório Institucional da UFSinstname:Universidade Federal de Sergipe (UFS)instacron:UFSinfo:eu-repo/semantics/openAccessORIGINALPEDRO_ELLISON_SANTOS_NASCIMENTO.pdfPEDRO_ELLISON_SANTOS_NASCIMENTO.pdfapplication/pdf3827507https://ri.ufs.br/jspui/bitstream/riufs/14905/2/PEDRO_ELLISON_SANTOS_NASCIMENTO.pdf25df50c2032602b726e6750b74156292MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81475https://ri.ufs.br/jspui/bitstream/riufs/14905/1/license.txt098cbbf65c2c15e1fb2e49c5d306a44cMD51TEXTPEDRO_ELLISON_SANTOS_NASCIMENTO.pdf.txtPEDRO_ELLISON_SANTOS_NASCIMENTO.pdf.txtExtracted texttext/plain171810https://ri.ufs.br/jspui/bitstream/riufs/14905/3/PEDRO_ELLISON_SANTOS_NASCIMENTO.pdf.txtefde54fe0d89b3a21a8273b1c88eb9a2MD53THUMBNAILPEDRO_ELLISON_SANTOS_NASCIMENTO.pdf.jpgPEDRO_ELLISON_SANTOS_NASCIMENTO.pdf.jpgGenerated Thumbnailimage/jpeg1460https://ri.ufs.br/jspui/bitstream/riufs/14905/4/PEDRO_ELLISON_SANTOS_NASCIMENTO.pdf.jpg40347add7fae3c609b382553c9630241MD54riufs/149052021-12-16 15:57:11.135oai:ufs.br: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Repositório InstitucionalPUBhttps://ri.ufs.br/oai/requestrepositorio@academico.ufs.bropendoar:2021-12-16T18:57:11Repositório Institucional da UFS - Universidade Federal de Sergipe (UFS)false
dc.title.pt_BR.fl_str_mv Caracterização por espectrometria de massas dos flavonoides extraídos por técnicas assistidas por ultrassom e micro-ondas das folhas de Croton grewioides Baill.
dc.title.alternative.eng.fl_str_mv Mass spectrometry in the characterization of flavonoids extracted by using microwave and ultrasound-assisted of leaves from Croton grewioides Baill.
title Caracterização por espectrometria de massas dos flavonoides extraídos por técnicas assistidas por ultrassom e micro-ondas das folhas de Croton grewioides Baill.
spellingShingle Caracterização por espectrometria de massas dos flavonoides extraídos por técnicas assistidas por ultrassom e micro-ondas das folhas de Croton grewioides Baill.
Nascimento, Pedro Ellison Santos do
Química
Ultrassom
Micro-ondas
Antioxidantes
Croton grewioides
Planejamento experimental
Extração assistida por ultrassom
Extração assistida por micro-ondas
Espectrometria de massas de alta resolução
Experimental design
Ultrasound-assisted extraction
Microwave-assisted-extraction
High resolution mass spectrometry
Antioxidants
CIENCIAS EXATAS E DA TERRA::QUIMICA
title_short Caracterização por espectrometria de massas dos flavonoides extraídos por técnicas assistidas por ultrassom e micro-ondas das folhas de Croton grewioides Baill.
title_full Caracterização por espectrometria de massas dos flavonoides extraídos por técnicas assistidas por ultrassom e micro-ondas das folhas de Croton grewioides Baill.
title_fullStr Caracterização por espectrometria de massas dos flavonoides extraídos por técnicas assistidas por ultrassom e micro-ondas das folhas de Croton grewioides Baill.
title_full_unstemmed Caracterização por espectrometria de massas dos flavonoides extraídos por técnicas assistidas por ultrassom e micro-ondas das folhas de Croton grewioides Baill.
title_sort Caracterização por espectrometria de massas dos flavonoides extraídos por técnicas assistidas por ultrassom e micro-ondas das folhas de Croton grewioides Baill.
author Nascimento, Pedro Ellison Santos do
author_facet Nascimento, Pedro Ellison Santos do
author_role author
dc.contributor.author.fl_str_mv Nascimento, Pedro Ellison Santos do
dc.contributor.advisor1.fl_str_mv Moraes, Valéria Regina de Souza
dc.contributor.advisor-co1.fl_str_mv Prado, Vilma Menezes de Jesus
contributor_str_mv Moraes, Valéria Regina de Souza
Prado, Vilma Menezes de Jesus
dc.subject.por.fl_str_mv Química
Ultrassom
Micro-ondas
Antioxidantes
Croton grewioides
Planejamento experimental
Extração assistida por ultrassom
Extração assistida por micro-ondas
Espectrometria de massas de alta resolução
topic Química
Ultrassom
Micro-ondas
Antioxidantes
Croton grewioides
Planejamento experimental
Extração assistida por ultrassom
Extração assistida por micro-ondas
Espectrometria de massas de alta resolução
Experimental design
Ultrasound-assisted extraction
Microwave-assisted-extraction
High resolution mass spectrometry
Antioxidants
CIENCIAS EXATAS E DA TERRA::QUIMICA
dc.subject.eng.fl_str_mv Experimental design
Ultrasound-assisted extraction
Microwave-assisted-extraction
High resolution mass spectrometry
Antioxidants
dc.subject.cnpq.fl_str_mv CIENCIAS EXATAS E DA TERRA::QUIMICA
description The complexity of compounds present in plants allows them to be used for different diseases, which is due to the presence of bioactive compounds named as secondary metabolites. Considering the lack of chemical knowledge of Croton grewioides species and the need to establish new methodologies for the extraction of its antioxidante compounds, in this work was carried out the optimization of the extraction processes by ultrasound and microwave using an experimental planning, aided by statistical analysis. Thus, it was possible to determine the best extraction conditions for each of the technique studied, ultrasound-assisted extraction (UAE) and microwave assisted extraction (MAE). From the best conditions of extraction, the extracts of C. grewioides were analyzed by liquid chromatography and their fingerprint chromatograms submitted to chemometric analysis. High resolution mass spectrometry analyzes were also carried out, using the Orbitrap analyzer, with the objective of obtaining a chemical profile of mass spectrums, chemical profile of the extracts, and try to chemically identify the molecular formula and propose the chemical structure to the largest number possible of compounds present in this species. Through the chemometric results from both fingerprint, the identified metabolites and the antioxidant activities obtained, it was possible to assigned that the best extraction technique for antioxidant compounds from C. grewioides is microwave assisted extraction. Additionally, it was possible to propose, for the first time, structures of forty one compounds in the extracts of this species from the analysis of high resolution mass spectrometry, being considered an unprecedented method for the chemical study of this species.
publishDate 2021
dc.date.accessioned.fl_str_mv 2021-12-16T18:57:11Z
dc.date.available.fl_str_mv 2021-12-16T18:57:11Z
dc.date.issued.fl_str_mv 2021-01-29
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv NASCIMENTO, Pedro Ellison Santos do. Caracterização por espectrometria de massas dos flavonoides extraídos por técnicas assistidas por ultrassom e micro-ondas das folhas de Croton grewioides Baill. 2021. 130 f. Dissertação (Mestrado em Química) – Universidade Federal de Sergipe, São Cristóvão, 2021.
dc.identifier.uri.fl_str_mv https://ri.ufs.br/jspui/handle/riufs/14905
identifier_str_mv NASCIMENTO, Pedro Ellison Santos do. Caracterização por espectrometria de massas dos flavonoides extraídos por técnicas assistidas por ultrassom e micro-ondas das folhas de Croton grewioides Baill. 2021. 130 f. Dissertação (Mestrado em Química) – Universidade Federal de Sergipe, São Cristóvão, 2021.
url https://ri.ufs.br/jspui/handle/riufs/14905
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dc.publisher.program.fl_str_mv Pós-Graduação em Química
dc.publisher.initials.fl_str_mv Universidade Federal de Sergipe
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFS
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reponame_str Repositório Institucional da UFS
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