Análise da ligação RAGE/HMGB1 no volume ósseo do reparo ósseo alveolar em camundongos

Detalhes bibliográficos
Autor(a) principal: Borges, Thainan de Santana
Data de Publicação: 2018
Tipo de documento: Trabalho de conclusão de curso
Idioma: por
Título da fonte: Repositório Institucional da UFS
Texto Completo: http://ri.ufs.br/jspui/handle/riufs/10111
Resumo: The bone repair development and neoformation processes are essential for tissue homeostasis after trauma or bone loss. Thus, modulation of immune / inflammatory response is a strategy to understand and improve this process. Interesting possibilities emerging to correlate immune system and bone system, after the discovery of inflammatory signaling mediated by the RAGE receptor and the HMGB1 ligand. In this way, this study aimed to elucidate the influence of the RAGE / HMGB1 complex in the immune / inflammatory response after exodontia of the upper incisor of C57Bl/7 mice.40 mice were divided into two groups [Control Group - WT; Group GLY - treated with glycyrrhizin (HMGB1 inhibitor)] and analyzed in 0, 7, 14, 21 days after exodontia. Histomorphometric analysis showed a very similar kinetics between WT and GLY groups in relation to the bone matrix. GLY group presented a lower density in the osteoblast volume and higher amount of osteoclast compared to control group at 7 and 14 days (p <0.05). Similarly, to volume density of osteoblasts, GLY presented lower bone density at day 21, and this density was higher when compared to WT mice. Therefore, inhibition of HMGB1 was not able to interfere with the kinetics of alveolar bone repair in mice, however showed differences in some components involved in the kinetics of alveolar bone repair.
id UFS-2_7f00c9042f37c184478fb8de3f9baf25
oai_identifier_str oai:ufs.br:riufs/10111
network_acronym_str UFS-2
network_name_str Repositório Institucional da UFS
repository_id_str
spelling Borges, Thainan de SantanaRepeke, Carlos Eduardo Palanch2018-12-13T13:17:56Z2018-12-13T13:17:56Z2018-09-24BORGES, Thainan de Santana. Análise da ligação RAGE/HMGB1 no volume ósseo do reparo ósseo alveolar em camundongos. 2018. Trabalho de Conclusão de Curso (Graduação em Odontologia) - Universidade Federal de Sergipe, Lagarto, 2018.http://ri.ufs.br/jspui/handle/riufs/10111The bone repair development and neoformation processes are essential for tissue homeostasis after trauma or bone loss. Thus, modulation of immune / inflammatory response is a strategy to understand and improve this process. Interesting possibilities emerging to correlate immune system and bone system, after the discovery of inflammatory signaling mediated by the RAGE receptor and the HMGB1 ligand. In this way, this study aimed to elucidate the influence of the RAGE / HMGB1 complex in the immune / inflammatory response after exodontia of the upper incisor of C57Bl/7 mice.40 mice were divided into two groups [Control Group - WT; Group GLY - treated with glycyrrhizin (HMGB1 inhibitor)] and analyzed in 0, 7, 14, 21 days after exodontia. Histomorphometric analysis showed a very similar kinetics between WT and GLY groups in relation to the bone matrix. GLY group presented a lower density in the osteoblast volume and higher amount of osteoclast compared to control group at 7 and 14 days (p <0.05). Similarly, to volume density of osteoblasts, GLY presented lower bone density at day 21, and this density was higher when compared to WT mice. Therefore, inhibition of HMGB1 was not able to interfere with the kinetics of alveolar bone repair in mice, however showed differences in some components involved in the kinetics of alveolar bone repair.A modulação da resposta imune/inflamatória é uma estratégia adotada para entender e melhorar o processo de reparo ósseo. Com a descoberta da sinalização inflamatória mediada pelo receptor RAGE e o ligante HMGB1, surgiram novas possibilidades de estudo entre a relação do sistema imune e o sistema ósseo. O objetivo foi elucidar a influencia do complexo RAGE/HMGB1 na densidade óssea do reparo ósseo alveolar após a exodontia do incisivo superior em camundongos. Foram utilizados 40 camundongos divididos em dois grupos [Grupo controle- WT; Grupo GLY – tratado com glicirrizina (inibidor de HMGB1)] e analisados após exodontia. Na análise histomorfométrica, em relação a matriz óssea foi observado uma cinética muito semelhante entre os grupos WT e GLY. Apensar de não demonstrar diferença significativa na formação óssea, o grupo GLY apresentou menores densidade no volume de osteoblastos e maiores de osteoclastos comparado ao grupo controle nos tempos de 7 e 14 dias (p<0,05). De forma similar a densidade de volume de osteoblastos, os camundongos GLY apresentaram menor densidade de tecido ósseo ao 21º dia, sendo que essa densidade foi maior quando comparados com os camundongos WT. Concluí-se que a inibição da proteína HMGB1 resultou em pequenas alterações na cinética de reparo ósseo alveolar em camundongos, apresentando diferenças no volume de osteoblastos e osteoclastos, componentes participantes da cinética do reparo ósseo alveolar.Lagarto, SEporProteínasInflamaçãoImunologiaProteína HMGB1HMGB1InflammationImmunologyOsteoimmunologyAnálise da ligação RAGE/HMGB1 no volume ósseo do reparo ósseo alveolar em camundongosinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/bachelorThesisUFSDOL - Departamento de Odontologia – Lagarto - Presencialreponame:Repositório Institucional da UFSinstname:Universidade Federal de Sergipe (UFS)instacron:UFSinfo:eu-repo/semantics/openAccessTEXTTHAINAN_DE_SANTANA_BORGES.pdf.txtTHAINAN_DE_SANTANA_BORGES.pdf.txtExtracted texttext/plain45512https://ri.ufs.br/jspui/bitstream/riufs/10111/3/THAINAN_DE_SANTANA_BORGES.pdf.txt98ac7e677b89953a070c5ce77d1ab758MD53THUMBNAILTHAINAN_DE_SANTANA_BORGES.pdf.jpgTHAINAN_DE_SANTANA_BORGES.pdf.jpgGenerated Thumbnailimage/jpeg1234https://ri.ufs.br/jspui/bitstream/riufs/10111/4/THAINAN_DE_SANTANA_BORGES.pdf.jpge1915f2b5cadeabdf0c9d35e6efa492bMD54LICENSElicense.txtlicense.txttext/plain; charset=utf-81475https://ri.ufs.br/jspui/bitstream/riufs/10111/1/license.txt098cbbf65c2c15e1fb2e49c5d306a44cMD51ORIGINALTHAINAN_DE_SANTANA_BORGES.pdfTHAINAN_DE_SANTANA_BORGES.pdfapplication/pdf753121https://ri.ufs.br/jspui/bitstream/riufs/10111/2/THAINAN_DE_SANTANA_BORGES.pdfd9773f0367bbb7b1efa9ddb69987c2e9MD52riufs/101112018-12-13 10:17:56.641oai:ufs.br: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Repositório InstitucionalPUBhttps://ri.ufs.br/oai/requestrepositorio@academico.ufs.bropendoar:2018-12-13T13:17:56Repositório Institucional da UFS - Universidade Federal de Sergipe (UFS)false
dc.title.pt_BR.fl_str_mv Análise da ligação RAGE/HMGB1 no volume ósseo do reparo ósseo alveolar em camundongos
title Análise da ligação RAGE/HMGB1 no volume ósseo do reparo ósseo alveolar em camundongos
spellingShingle Análise da ligação RAGE/HMGB1 no volume ósseo do reparo ósseo alveolar em camundongos
Borges, Thainan de Santana
Proteínas
Inflamação
Imunologia
Proteína HMGB1
HMGB1
Inflammation
Immunology
Osteoimmunology
title_short Análise da ligação RAGE/HMGB1 no volume ósseo do reparo ósseo alveolar em camundongos
title_full Análise da ligação RAGE/HMGB1 no volume ósseo do reparo ósseo alveolar em camundongos
title_fullStr Análise da ligação RAGE/HMGB1 no volume ósseo do reparo ósseo alveolar em camundongos
title_full_unstemmed Análise da ligação RAGE/HMGB1 no volume ósseo do reparo ósseo alveolar em camundongos
title_sort Análise da ligação RAGE/HMGB1 no volume ósseo do reparo ósseo alveolar em camundongos
author Borges, Thainan de Santana
author_facet Borges, Thainan de Santana
author_role author
dc.contributor.author.fl_str_mv Borges, Thainan de Santana
dc.contributor.advisor1.fl_str_mv Repeke, Carlos Eduardo Palanch
contributor_str_mv Repeke, Carlos Eduardo Palanch
dc.subject.por.fl_str_mv Proteínas
Inflamação
Imunologia
Proteína HMGB1
topic Proteínas
Inflamação
Imunologia
Proteína HMGB1
HMGB1
Inflammation
Immunology
Osteoimmunology
dc.subject.eng.fl_str_mv HMGB1
Inflammation
Immunology
Osteoimmunology
description The bone repair development and neoformation processes are essential for tissue homeostasis after trauma or bone loss. Thus, modulation of immune / inflammatory response is a strategy to understand and improve this process. Interesting possibilities emerging to correlate immune system and bone system, after the discovery of inflammatory signaling mediated by the RAGE receptor and the HMGB1 ligand. In this way, this study aimed to elucidate the influence of the RAGE / HMGB1 complex in the immune / inflammatory response after exodontia of the upper incisor of C57Bl/7 mice.40 mice were divided into two groups [Control Group - WT; Group GLY - treated with glycyrrhizin (HMGB1 inhibitor)] and analyzed in 0, 7, 14, 21 days after exodontia. Histomorphometric analysis showed a very similar kinetics between WT and GLY groups in relation to the bone matrix. GLY group presented a lower density in the osteoblast volume and higher amount of osteoclast compared to control group at 7 and 14 days (p <0.05). Similarly, to volume density of osteoblasts, GLY presented lower bone density at day 21, and this density was higher when compared to WT mice. Therefore, inhibition of HMGB1 was not able to interfere with the kinetics of alveolar bone repair in mice, however showed differences in some components involved in the kinetics of alveolar bone repair.
publishDate 2018
dc.date.accessioned.fl_str_mv 2018-12-13T13:17:56Z
dc.date.available.fl_str_mv 2018-12-13T13:17:56Z
dc.date.issued.fl_str_mv 2018-09-24
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/bachelorThesis
format bachelorThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv BORGES, Thainan de Santana. Análise da ligação RAGE/HMGB1 no volume ósseo do reparo ósseo alveolar em camundongos. 2018. Trabalho de Conclusão de Curso (Graduação em Odontologia) - Universidade Federal de Sergipe, Lagarto, 2018.
dc.identifier.uri.fl_str_mv http://ri.ufs.br/jspui/handle/riufs/10111
identifier_str_mv BORGES, Thainan de Santana. Análise da ligação RAGE/HMGB1 no volume ósseo do reparo ósseo alveolar em camundongos. 2018. Trabalho de Conclusão de Curso (Graduação em Odontologia) - Universidade Federal de Sergipe, Lagarto, 2018.
url http://ri.ufs.br/jspui/handle/riufs/10111
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.initials.fl_str_mv UFS
dc.publisher.department.fl_str_mv DOL - Departamento de Odontologia – Lagarto - Presencial
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFS
instname:Universidade Federal de Sergipe (UFS)
instacron:UFS
instname_str Universidade Federal de Sergipe (UFS)
instacron_str UFS
institution UFS
reponame_str Repositório Institucional da UFS
collection Repositório Institucional da UFS
bitstream.url.fl_str_mv https://ri.ufs.br/jspui/bitstream/riufs/10111/3/THAINAN_DE_SANTANA_BORGES.pdf.txt
https://ri.ufs.br/jspui/bitstream/riufs/10111/4/THAINAN_DE_SANTANA_BORGES.pdf.jpg
https://ri.ufs.br/jspui/bitstream/riufs/10111/1/license.txt
https://ri.ufs.br/jspui/bitstream/riufs/10111/2/THAINAN_DE_SANTANA_BORGES.pdf
bitstream.checksum.fl_str_mv 98ac7e677b89953a070c5ce77d1ab758
e1915f2b5cadeabdf0c9d35e6efa492b
098cbbf65c2c15e1fb2e49c5d306a44c
d9773f0367bbb7b1efa9ddb69987c2e9
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da UFS - Universidade Federal de Sergipe (UFS)
repository.mail.fl_str_mv repositorio@academico.ufs.br
_version_ 1802110818325626880