Growth inhibition of sulfate-reducing bacteria in produced water from the petroleum industry using essential oils

• Autor(a) principal: Souza, Pamella Macedo de
• Data de Publicação: 2017
• Outros Autores: Goulart, Fatima Regina de Vasconcelos, Marques, Joana Montezano, Bizzo, Humberto Ribeiro, Blank, Arie Fitzgerald, Silveira, Claudia Julia Groposo, Sousa, Maira Paula de, Santos, Vanessa Vólaro Caminha Mota dos, Alviano, Celuta Sales, Moreno, Daniela Sales Alviano, Seldin, Lucy
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UFS
• Texto Completo: http://ri.ufs.br/jspui/handle/riufs/7244
• topic: Essential oils; Antimicrobial activity; Sulfate-reducing bacteria (SRB); Production water; Oil industry; Bactérias redutoras de sulfato; Óleos essenciais

Strategies for the control of sulfate-reducing bacteria (SRB) in the oil industry involve the use of high concentrations of biocides, but these may induce bacterial resistance and/or be harmful to public health and the environment. Essential oils (EO) produced by plants inhibit the growth of different microorganisms and are a possible alternative for controlling SRB. We aimed to characterize the bacterial community of produced water obtained from a Brazilian petroleum facility using molecular methods, as well as to evaluate the antimicrobial activity of EO from different plants and their major components against Desulfovibrio alaskensis NCIMB 13491 and against SRB growth directly in the produced water. Denaturing gradient gel electrophoresis revealed the presence of the genera Pelobacter and Marinobacterium, Geotoga petraea, and the SRB Desulfoplanes formicivorans in our produced water samples. Sequencing of dsrA insert-containing clones confirmed the presence of sequences related to D. formicivorans. EO obtained from Citrus aurantifolia, Lippia alba LA44 and Cymbopogon citratus, as well as citral, linalool, eugenol and geraniol, greatly inhibited (minimum inhibitory concentration (MIC) = 78 µg/mL) the growth of D. alaskensis in a liquid medium. The same MIC was obtained directly in the produced water with EO from L. alba LA44 (containing 82% citral) and with pure citral. These findings may help to control detrimental bacteria in the oil industry.