Caracterização físico-química e avaliação do efeito de esponjas de alginato-gelatina-ácido úsnico sobre a cicatrização em rato
Autor(a) principal: | |
---|---|
Data de Publicação: | 2012 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFS |
Texto Completo: | https://ri.ufs.br/handle/riufs/3849 |
Resumo: | There is interest in the pharmaceutical area in the use of sponges, produced with biopolymers, as matrices for the controlled release of drugs. The biopolymers for pharmaceutical applications must have physicochemical and appropriate mechanical and biocompatibility. Alginate is a biopolymer, obtained from the alginic acid, which has been largely studied due to its ability to incorporate and promote the controlled release of drugs. Gelatin is a protein obtained by hydrolysis of collagen and has been used in obtaining produce hydrogels and sponges with therapeutic properties. Usnic acid (UA) is the most abundant metabolite of the lichen Cladonia substellata has proven antimicrobial, anti-inflammatory, analgesic and healing activities. In this context, the aim of this work was to physicochemical characterization and evaluate the effect of alginate-gelatin sponges, containing usnic acid on wound healing in rats. The sponges were prepared from gelatin, alginate and incorporated with usnic acid in concentrations of 1 and 5% (m/v) and freeze dried. For material characterization were used thermogravimetry/derivative thermogravimetry (TG/DTG), differential scanning calorimetry (DSC), infrared absorption spectroscopy (FTIR), scanning electron microscopy (SEM) and mechanical properties tests. The biological assay was performed with 36 rats divided into three groups: without treatment (CTR); treated with inert sponge (ALG/GEL); and treated with the sponge containing usnic acid 5% (AU 5%). After 7 and 14 days, six animals from each group were submitted to euthanasia for histological analysis. The average content of usnic acid was 60.03 and 97.38% for sponges in 1 and 5%, respectively. DSC analysis results show the absence of endothermic peak of UA pure, indicating that the drug was incorporated into the polymer system. TG/DTG curves corroborate with the DSC data and showed that the incorporation of usnic acid has not changed the thermal behavior of sponges. The FTIR spectra of sponges showed a sum corresponding to the event of usnic acid, alginate and gelatin bands, indicating the presence of these compounds in the system. The analysis by SEM showed that the incorporation of different concentrations of UA, in sponges promotes changes in microscopic structure, resulting in different mechanical behaviors. The biological assays in 7 days, showed an inflammatory reaction in the marginal and central portions of the wound, but ALG/ GEL and AU 5% groups, promoted an acceleration in the formation of granulation tissue. In 14 days, in the AU 5% group, was a clear reduction of the inflammatory component even in the central portion of the wound. Additionally, there was a marked reduction in vascular component, when compared to the CTR group with a homogeneous pattern of colagenization type III in the entire length of the scar. In conclusion, the incorporation of usnic acid has not changed the thermal stability of sponges and, in addition, the use of alginate-gelatin sponge and promotes the wound healing and the incorporation of the drug eases wound contraction and a homogeneous deposition of collagen, avoiding the formation of hipertrophic scars. |
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Porto Neto, Aldino do Nascimentohttp://lattes.cnpq.br/5393215265663330Silva, Francilene Amaral dahttp://lattes.cnpq.br/78758845958469162017-09-26T12:18:04Z2017-09-26T12:18:04Z2012-08-24PORTO NETO, Aldino do Nascimento. Physicochemical characterization and evaluation of the effect of alginate-gelatin-usnic acid sponges of wound healing in rats. 2012. 105 f. Dissertação (Mestrado em Ciências da Saúde) - Universidade Federal de Sergipe, Aracaju, 2012.https://ri.ufs.br/handle/riufs/3849There is interest in the pharmaceutical area in the use of sponges, produced with biopolymers, as matrices for the controlled release of drugs. The biopolymers for pharmaceutical applications must have physicochemical and appropriate mechanical and biocompatibility. Alginate is a biopolymer, obtained from the alginic acid, which has been largely studied due to its ability to incorporate and promote the controlled release of drugs. Gelatin is a protein obtained by hydrolysis of collagen and has been used in obtaining produce hydrogels and sponges with therapeutic properties. Usnic acid (UA) is the most abundant metabolite of the lichen Cladonia substellata has proven antimicrobial, anti-inflammatory, analgesic and healing activities. In this context, the aim of this work was to physicochemical characterization and evaluate the effect of alginate-gelatin sponges, containing usnic acid on wound healing in rats. The sponges were prepared from gelatin, alginate and incorporated with usnic acid in concentrations of 1 and 5% (m/v) and freeze dried. For material characterization were used thermogravimetry/derivative thermogravimetry (TG/DTG), differential scanning calorimetry (DSC), infrared absorption spectroscopy (FTIR), scanning electron microscopy (SEM) and mechanical properties tests. The biological assay was performed with 36 rats divided into three groups: without treatment (CTR); treated with inert sponge (ALG/GEL); and treated with the sponge containing usnic acid 5% (AU 5%). After 7 and 14 days, six animals from each group were submitted to euthanasia for histological analysis. The average content of usnic acid was 60.03 and 97.38% for sponges in 1 and 5%, respectively. DSC analysis results show the absence of endothermic peak of UA pure, indicating that the drug was incorporated into the polymer system. TG/DTG curves corroborate with the DSC data and showed that the incorporation of usnic acid has not changed the thermal behavior of sponges. The FTIR spectra of sponges showed a sum corresponding to the event of usnic acid, alginate and gelatin bands, indicating the presence of these compounds in the system. The analysis by SEM showed that the incorporation of different concentrations of UA, in sponges promotes changes in microscopic structure, resulting in different mechanical behaviors. The biological assays in 7 days, showed an inflammatory reaction in the marginal and central portions of the wound, but ALG/ GEL and AU 5% groups, promoted an acceleration in the formation of granulation tissue. In 14 days, in the AU 5% group, was a clear reduction of the inflammatory component even in the central portion of the wound. Additionally, there was a marked reduction in vascular component, when compared to the CTR group with a homogeneous pattern of colagenization type III in the entire length of the scar. In conclusion, the incorporation of usnic acid has not changed the thermal stability of sponges and, in addition, the use of alginate-gelatin sponge and promotes the wound healing and the incorporation of the drug eases wound contraction and a homogeneous deposition of collagen, avoiding the formation of hipertrophic scars.Existe um interesse na área farmacêutica no uso de esponjas, produzidas com biopolímeros, como matrizes para a liberação controlada de fármacos. Os biopolímeros para aplicações farmacêuticas devem possuir propriedades físico-químicas e mecânicas adequadas, além de serem biocompatíveis. O alginato é um biopolímero, obtido a partir do ácido algínico, que tem sido largamente estudado devido a sua capacidade de incorporar compostos e promover a liberação controlada de fármacos. A gelatina é uma proteína obtida pela hidrólise do colágeno e vem sendo empregada na obtenção de hidrogéis e esponjas com propriedades terapêuticas. O ácido úsnico (AU) é o metabólito mais abundante do líquen Cladonia substellata e possui comprovada atividade antimicrobiana, anti-inflamatória, analgésica e cicatrizante. Neste contexto, o objetivo deste trabalho foi caracterizar físico-quimicamente e avaliar o efeito de esponjas de alginato-gelatina, contendo ácido úsnico sob a cicatrização em ratos. As esponjas foram preparadas a partir do alginato e gelatina, incorporadas com ácido úsnico, nas concentrações de 1 e 5% (m/v) e liofilizadas. Para caracterização do material foram utilizadas a análise termogravimétrica/termogravimétrica derivada (TG/DTG), a calorimetria exploratória diferencial (DSC), espectroscopia de absorção na região do infravermelho (FTIR), microscopia eletrônica de varredura (MEV) e testes de propriedades mecânicas. O ensaio biológico foi realizado com 36 ratos divididos em três grupos: sem tratamento (CTR); tratados com esponja inerte (ALG/GEL); e tratados com esponja contendo ácido úsnico a 5% (AU 5%). Após 7 e 14 dias, seis animais de cada grupo foram eutanasiados para análise histológica. O teor médio de ácido úsnico foi de 60,03 e 97,38% nas esponjas de 1 e 5%, respectivamente. Os resultados da análise de DSC mostram a ausência do pico endotérmico referente ao AU puro, indicando que o fármaco foi incorporado ao sistema polimérico. As curvas TG/DTG corroboram com os dados do DSC e mostraram que a incorporação do ácido úsnico não alterou o perfil geral da decomposição térmica das esponjas. Os espectros FTIR das esponjas mostraram um somatório de eventos correspondentes às bandas do ácido úsnico, alginato e gelatina, indicando a presença dos referidos compostos no sistema. A análise por MEV mostrou, que a incorporação de diferentes concentrações de AU, nas esponjas promove alterações na estrutura microscópica, resultando em diferentes comportamentos mecânicos. Os ensaios biológicos em 7 dias, mostram uma reação inflamatória nas porções centrais e marginais da ferida, porém os grupos ALG/GEL e AU 5%, promoveram uma aceleração na formação de tecido de granulação mais maduro. Em 14 dias, no grupo AU 5%, foi nítida a redução do componente inflamatório mesmo na porção central da ferida. Adicionalmente, observou-se uma marcante redução na rede vascular, quando comparado ao grupo CTR, acompanhada de um padrão homogêneo de colagenização tipo III em toda a extensão da cicatriz. Em conclusão, a incorporação do ácido úsnico não alterou a estabilidade térmica das esponjas e, além disso, o uso de esponja de alginato e gelatina favorece a cicatrização de feridas e a incorporação do fármaco facilita a contração da ferida e uma deposição homogênea de colágeno, evitando a formação de cicatrizes antiestéticas.application/pdfporUniversidade Federal de SergipePós-Graduação em Ciências da SaúdeUFSBRBiomateriaisEsponjasÁcido úsnicoCaracterização físico-químicaCicatrizaçãoBiomaterialsSpongesUsnic acidPhysicochemical characterizationWound healingCNPQ::CIENCIAS DA SAUDE::MEDICINACaracterização físico-química e avaliação do efeito de esponjas de alginato-gelatina-ácido úsnico sobre a cicatrização em ratoPhysicochemical characterization and evaluation of the effect of alginate-gelatin-usnic acid sponges of wound healing in ratsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFSinstname:Universidade Federal de Sergipe (UFS)instacron:UFSTEXTALDINO_NASCIMENTO_PORTO_NETO.pdf.txtALDINO_NASCIMENTO_PORTO_NETO.pdf.txtExtracted texttext/plain184928https://ri.ufs.br/jspui/bitstream/riufs/3849/2/ALDINO_NASCIMENTO_PORTO_NETO.pdf.txtdb0c14f63507a778ecc36447bf57adc7MD52THUMBNAILALDINO_NASCIMENTO_PORTO_NETO.pdf.jpgALDINO_NASCIMENTO_PORTO_NETO.pdf.jpgGenerated Thumbnailimage/jpeg1399https://ri.ufs.br/jspui/bitstream/riufs/3849/3/ALDINO_NASCIMENTO_PORTO_NETO.pdf.jpg767eb1c62cb30335e14f06b361dbdcf0MD53ORIGINALALDINO_NASCIMENTO_PORTO_NETO.pdfapplication/pdf6498485https://ri.ufs.br/jspui/bitstream/riufs/3849/1/ALDINO_NASCIMENTO_PORTO_NETO.pdf8c698d55ca8d65ce04c86c8acf7458f5MD51riufs/38492017-11-28 16:21:02.102oai:ufs.br:riufs/3849Repositório InstitucionalPUBhttps://ri.ufs.br/oai/requestrepositorio@academico.ufs.bropendoar:2017-11-28T19:21:02Repositório Institucional da UFS - Universidade Federal de Sergipe (UFS)false |
dc.title.por.fl_str_mv |
Caracterização físico-química e avaliação do efeito de esponjas de alginato-gelatina-ácido úsnico sobre a cicatrização em rato |
dc.title.alternative.eng.fl_str_mv |
Physicochemical characterization and evaluation of the effect of alginate-gelatin-usnic acid sponges of wound healing in rats |
title |
Caracterização físico-química e avaliação do efeito de esponjas de alginato-gelatina-ácido úsnico sobre a cicatrização em rato |
spellingShingle |
Caracterização físico-química e avaliação do efeito de esponjas de alginato-gelatina-ácido úsnico sobre a cicatrização em rato Porto Neto, Aldino do Nascimento Biomateriais Esponjas Ácido úsnico Caracterização físico-química Cicatrização Biomaterials Sponges Usnic acid Physicochemical characterization Wound healing CNPQ::CIENCIAS DA SAUDE::MEDICINA |
title_short |
Caracterização físico-química e avaliação do efeito de esponjas de alginato-gelatina-ácido úsnico sobre a cicatrização em rato |
title_full |
Caracterização físico-química e avaliação do efeito de esponjas de alginato-gelatina-ácido úsnico sobre a cicatrização em rato |
title_fullStr |
Caracterização físico-química e avaliação do efeito de esponjas de alginato-gelatina-ácido úsnico sobre a cicatrização em rato |
title_full_unstemmed |
Caracterização físico-química e avaliação do efeito de esponjas de alginato-gelatina-ácido úsnico sobre a cicatrização em rato |
title_sort |
Caracterização físico-química e avaliação do efeito de esponjas de alginato-gelatina-ácido úsnico sobre a cicatrização em rato |
author |
Porto Neto, Aldino do Nascimento |
author_facet |
Porto Neto, Aldino do Nascimento |
author_role |
author |
dc.contributor.author.fl_str_mv |
Porto Neto, Aldino do Nascimento |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/5393215265663330 |
dc.contributor.advisor1.fl_str_mv |
Silva, Francilene Amaral da |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/7875884595846916 |
contributor_str_mv |
Silva, Francilene Amaral da |
dc.subject.por.fl_str_mv |
Biomateriais Esponjas Ácido úsnico Caracterização físico-química Cicatrização |
topic |
Biomateriais Esponjas Ácido úsnico Caracterização físico-química Cicatrização Biomaterials Sponges Usnic acid Physicochemical characterization Wound healing CNPQ::CIENCIAS DA SAUDE::MEDICINA |
dc.subject.eng.fl_str_mv |
Biomaterials Sponges Usnic acid Physicochemical characterization Wound healing |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS DA SAUDE::MEDICINA |
description |
There is interest in the pharmaceutical area in the use of sponges, produced with biopolymers, as matrices for the controlled release of drugs. The biopolymers for pharmaceutical applications must have physicochemical and appropriate mechanical and biocompatibility. Alginate is a biopolymer, obtained from the alginic acid, which has been largely studied due to its ability to incorporate and promote the controlled release of drugs. Gelatin is a protein obtained by hydrolysis of collagen and has been used in obtaining produce hydrogels and sponges with therapeutic properties. Usnic acid (UA) is the most abundant metabolite of the lichen Cladonia substellata has proven antimicrobial, anti-inflammatory, analgesic and healing activities. In this context, the aim of this work was to physicochemical characterization and evaluate the effect of alginate-gelatin sponges, containing usnic acid on wound healing in rats. The sponges were prepared from gelatin, alginate and incorporated with usnic acid in concentrations of 1 and 5% (m/v) and freeze dried. For material characterization were used thermogravimetry/derivative thermogravimetry (TG/DTG), differential scanning calorimetry (DSC), infrared absorption spectroscopy (FTIR), scanning electron microscopy (SEM) and mechanical properties tests. The biological assay was performed with 36 rats divided into three groups: without treatment (CTR); treated with inert sponge (ALG/GEL); and treated with the sponge containing usnic acid 5% (AU 5%). After 7 and 14 days, six animals from each group were submitted to euthanasia for histological analysis. The average content of usnic acid was 60.03 and 97.38% for sponges in 1 and 5%, respectively. DSC analysis results show the absence of endothermic peak of UA pure, indicating that the drug was incorporated into the polymer system. TG/DTG curves corroborate with the DSC data and showed that the incorporation of usnic acid has not changed the thermal behavior of sponges. The FTIR spectra of sponges showed a sum corresponding to the event of usnic acid, alginate and gelatin bands, indicating the presence of these compounds in the system. The analysis by SEM showed that the incorporation of different concentrations of UA, in sponges promotes changes in microscopic structure, resulting in different mechanical behaviors. The biological assays in 7 days, showed an inflammatory reaction in the marginal and central portions of the wound, but ALG/ GEL and AU 5% groups, promoted an acceleration in the formation of granulation tissue. In 14 days, in the AU 5% group, was a clear reduction of the inflammatory component even in the central portion of the wound. Additionally, there was a marked reduction in vascular component, when compared to the CTR group with a homogeneous pattern of colagenization type III in the entire length of the scar. In conclusion, the incorporation of usnic acid has not changed the thermal stability of sponges and, in addition, the use of alginate-gelatin sponge and promotes the wound healing and the incorporation of the drug eases wound contraction and a homogeneous deposition of collagen, avoiding the formation of hipertrophic scars. |
publishDate |
2012 |
dc.date.issued.fl_str_mv |
2012-08-24 |
dc.date.accessioned.fl_str_mv |
2017-09-26T12:18:04Z |
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2017-09-26T12:18:04Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
PORTO NETO, Aldino do Nascimento. Physicochemical characterization and evaluation of the effect of alginate-gelatin-usnic acid sponges of wound healing in rats. 2012. 105 f. Dissertação (Mestrado em Ciências da Saúde) - Universidade Federal de Sergipe, Aracaju, 2012. |
dc.identifier.uri.fl_str_mv |
https://ri.ufs.br/handle/riufs/3849 |
identifier_str_mv |
PORTO NETO, Aldino do Nascimento. Physicochemical characterization and evaluation of the effect of alginate-gelatin-usnic acid sponges of wound healing in rats. 2012. 105 f. Dissertação (Mestrado em Ciências da Saúde) - Universidade Federal de Sergipe, Aracaju, 2012. |
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https://ri.ufs.br/handle/riufs/3849 |
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Universidade Federal de Sergipe |
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Pós-Graduação em Ciências da Saúde |
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UFS |
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BR |
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Universidade Federal de Sergipe |
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