Análises química, antioxidante e e citotoxicidade em linhagens tumorais de germoplasma de Eplingiella fruticosa
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFS |
Texto Completo: | https://ri.ufs.br/jspui/handle/riufs/17970 |
Resumo: | Eplingiella fruticosa is an aromatic plant of the Lamiaceae family known as “alecrim-devaqueiro”. Its popular use has aroused the interest of the scientific community regarding the possible pharmacological properties involved, and the anti-hyperalgesic, antinociceptive, antiinflammatory, vasodilatory and anti-neurodegenerative effects have already been attributed to its essential oil and extracts. The first article investigated the variations in the chemical profiles and biological activities (antioxidant and cytotoxicity) of Eplingiella fruticosa from the State of Sergipe. The essential oils of six populations were extracted by hydrodistillation and analyzed by Gas Chromatography coupled to a Mass Spectrometer and a Flame Ionization Detector (GC/EM-DIC). The antioxidant activity of the essential oils was tested by different assays (DPPH, ABTS, β-carotene and FRAP) and the cytotoxic activity was determined by the SRB assay. The compounds found in greater amounts were: α-pinene, β-pinene, 1,8-cineole, camphor, borneol, δ-elemene, α-cubebene, α-ylangeno, (E)-caryophyllene, germacrene D, bicyclogermacrene, trans -calamenene, spathulenol, caryophyllene oxide and viridiflorol. These compounds defined the formation of two groups. The first group consisted of the populations of São Cristóvão, Itaporanga, Japaratuba and Malhada dos Bois and was characterized by the presence of camphor monoterpene (8.39-11.27%) as the highest content compound in relation to the other municipalities. The second group consisted of the populations of Moita Bonita and Pirambu and was characterized by the majority presence of bicyclogermacrene sesquiterpene (7.45% and 10.98%). The plants showed weak effects in terms of antioxidant activity, but the essential oil showed significant toxicity for the A549 strains (51.00% cell viability) in the population of Japaratuba and B16F10 (64.94.6% of cell viability) in Malhada of the Oxen. The second article analyzed the chemical profile, antioxidant activity and cytotoxicity of aqueous and ethanolic extracts from populations of E. fruticosa. The extracts were analyzed by High Performance Liquid Chromatography (HPLC). Antioxidant activity and cytotoxic activity were tested in the same way as in the first article. The flavonoid quercitrin was identified in the ethanol extract. The aqueous extracts showed higher antioxidant activity in relation to the other extracts. The DPPH and ABTS radicals range from 84.89- 75.62% and 85.21-64.04%, respectively, in the aqueous extracts. The ethanol extracts from the populations of Japaratuba, São Cristóvão and Itaporanga d'Ajuda showed the highest inhibitory activities with 90.63%, 90.53% and 88.47% for the C6 strain and the hydrodistilled ethanol extract from the Japaratuba population showed high growth inhibition at A549 (87.17% cell viability) and C6 (72.83% cell viability). The observations from these studies show that E. fruticosa can be a significant source of medicinally important natural compounds. |
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Pinto, Jéssika Andreza OliveiraArrigoni-Blank, Maria de Fátima2023-07-28T22:28:15Z2023-07-28T22:28:15Z2022-01-28PINTO, Jéssika Andreza Oliveira. Análises química, antioxidante e citotoxicidade em linhagens tumorais de germoplasma de Eplingiella fruticosa. 2022. 66 f. Tese (Doutorado em Agricultura e Biodiversidade) - Universidade Federal de Sergipe, São Cristóvão, SE, 2022.https://ri.ufs.br/jspui/handle/riufs/17970Eplingiella fruticosa is an aromatic plant of the Lamiaceae family known as “alecrim-devaqueiro”. Its popular use has aroused the interest of the scientific community regarding the possible pharmacological properties involved, and the anti-hyperalgesic, antinociceptive, antiinflammatory, vasodilatory and anti-neurodegenerative effects have already been attributed to its essential oil and extracts. The first article investigated the variations in the chemical profiles and biological activities (antioxidant and cytotoxicity) of Eplingiella fruticosa from the State of Sergipe. The essential oils of six populations were extracted by hydrodistillation and analyzed by Gas Chromatography coupled to a Mass Spectrometer and a Flame Ionization Detector (GC/EM-DIC). The antioxidant activity of the essential oils was tested by different assays (DPPH, ABTS, β-carotene and FRAP) and the cytotoxic activity was determined by the SRB assay. The compounds found in greater amounts were: α-pinene, β-pinene, 1,8-cineole, camphor, borneol, δ-elemene, α-cubebene, α-ylangeno, (E)-caryophyllene, germacrene D, bicyclogermacrene, trans -calamenene, spathulenol, caryophyllene oxide and viridiflorol. These compounds defined the formation of two groups. The first group consisted of the populations of São Cristóvão, Itaporanga, Japaratuba and Malhada dos Bois and was characterized by the presence of camphor monoterpene (8.39-11.27%) as the highest content compound in relation to the other municipalities. The second group consisted of the populations of Moita Bonita and Pirambu and was characterized by the majority presence of bicyclogermacrene sesquiterpene (7.45% and 10.98%). The plants showed weak effects in terms of antioxidant activity, but the essential oil showed significant toxicity for the A549 strains (51.00% cell viability) in the population of Japaratuba and B16F10 (64.94.6% of cell viability) in Malhada of the Oxen. The second article analyzed the chemical profile, antioxidant activity and cytotoxicity of aqueous and ethanolic extracts from populations of E. fruticosa. The extracts were analyzed by High Performance Liquid Chromatography (HPLC). Antioxidant activity and cytotoxic activity were tested in the same way as in the first article. The flavonoid quercitrin was identified in the ethanol extract. The aqueous extracts showed higher antioxidant activity in relation to the other extracts. The DPPH and ABTS radicals range from 84.89- 75.62% and 85.21-64.04%, respectively, in the aqueous extracts. The ethanol extracts from the populations of Japaratuba, São Cristóvão and Itaporanga d'Ajuda showed the highest inhibitory activities with 90.63%, 90.53% and 88.47% for the C6 strain and the hydrodistilled ethanol extract from the Japaratuba population showed high growth inhibition at A549 (87.17% cell viability) and C6 (72.83% cell viability). The observations from these studies show that E. fruticosa can be a significant source of medicinally important natural compounds.A Eplingiella fruticosa é uma planta aromática da família Lamiaceae conhecida como “alecrimde-vaqueiro”. Seu uso popular tem despertado interesse da comunidade científica quanto às possíveis propriedades farmacológicas envolvidas, e os efeitos anti-hiperalgésico, antinociceptivo, anti-inflamatório, vasodilatador e contra doenças neurodegenerativas já foram atribuídos ao seu óleo essencial e extratos. O primeiro artigo investigou as variações nos perfis químicos e atividades biológicas (antioxidante e citotoxicidade) de Eplingiella fruticosa do estado de Sergipe. Os óleos essenciais de seis populações foram extraídos por hidrodestilação e analisados por Cromatografia Gasosa acoplada a um Espectrômetro de Massa e a um Detector de Ionização em Chama (CG/EM-DIC). A atividade antioxidante dos óleos essenciais foi testada por diferentes ensaios (DPPH, ABTS, β-caroteno e FRAP) e a atividade citotóxica foi feita pelo ensaio SRB. Os compostos encontrados em maiores quantidades foram: α-pineno, βpineno, 1,8-cineol, cânfora, borneol, δ-elemeno, α-cubebeno, α-ylangeno, (E)-cariofileno, germacreno D, biciclogermacreno, trans-calameneno, espatulenol, óxido de cariofileno e viridiflorol. Esses compostos definiram a formação de dois grupos. O primeiro grupo foi constituído pelas populações de São Cristóvão, Itaporanga, Japaratuba e Malhada dos Bois e caracterizou-se pela presença do monoterpeno cânfora (8,39-11,27%) como composto de maior teor em relação aos demais municípios. O segundo grupo foi constituído pelas populações de Moita Bonita e Pirambu e se caracterizou pela presença majoritária do sesquiterpeno biciclogermacreno (7,45% e 10,98%). As plantas apresentaram efeitos fracos em termos de atividade antioxidante, porém o óleo essencial mostrou toxicidade significativa para as linhagens A549 (51,00% da viabilidade celular) na população de Japaratuba e B16F10 (64,94,6% da viabilidade celular) em Malhada dos Bois. O segundo artigo analisou o perfil químico, a atividade antioxidante e citotoxicidade de extratos aquosos e etanólicos das populações de E. fruticosa. Os extratos foram analisados por Cromatografia Líquida de Alta Eficiência (CLAE). A atividade antioxidante e a atividade citotóxica foram testadas do mesmo modo do primeiro artigo. O flavonoide quercitrina foi identificado no extrato etanólico. Os extratos aquosos apresentaram maior atividade antioxidante em relação aos demais extratos. Os radicais DPPH e ABTS variaram de 84,89-75,62% e 85,21-64,04%, respectivamente, nos extratos aquosos. Os extratos etanólicos das populações de Japaratuba, São Cristóvão e Itaporanga d’Ajuda apresentaram as maiores atividades inibitórias com 90,63%, 90,53% e 88,47% para a linhagem C6 e o extrato etanólico hidrodestilado da população de Japaratuba apresentou alta inibição de crescimento em A549 (87,17% da viabilidade celular) e C6 (72,83% da viabilidade celular). As observações destes estudos mostram que E. fruticosa pode ser uma fonte significativa de compostos naturais importantes do ponto de vista medicinal.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESporEplingiella fruticosaÓleo essencialExtratosAtividade biológicaAlecrim-de-vaqueiroEssential oilBiological activityExtractsCIENCIAS AGRARIASAnálises química, antioxidante e e citotoxicidade em linhagens tumorais de germoplasma de Eplingiella fruticosainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisPós-Graduação em Agricultura e BiodiversidadeUniversidade Federal de Sergipereponame:Repositório Institucional da UFSinstname:Universidade Federal de Sergipe (UFS)instacron:UFSinfo:eu-repo/semantics/openAccessLICENSElicense.txtlicense.txttext/plain; charset=utf-81475https://ri.ufs.br/jspui/bitstream/riufs/17970/1/license.txt098cbbf65c2c15e1fb2e49c5d306a44cMD51ORIGINALJESSIKA_ANDREZA_OLIVEIRA_PINTO.pdfJESSIKA_ANDREZA_OLIVEIRA_PINTO.pdfapplication/pdf2792719https://ri.ufs.br/jspui/bitstream/riufs/17970/2/JESSIKA_ANDREZA_OLIVEIRA_PINTO.pdf95310d147c96fe87cd28af557acff507MD52TEXTJÉSSIKA_ANDREZA_OLIVEIRA_PINTO.pdf.txtJÉSSIKA_ANDREZA_OLIVEIRA_PINTO.pdf.txtExtracted texttext/plain180764https://ri.ufs.br/jspui/bitstream/riufs/17970/3/J%c3%89SSIKA_ANDREZA_OLIVEIRA_PINTO.pdf.txtc9c5a6efde0d5eb44461cbae81b0201cMD53JESSIKA_ANDREZA_OLIVEIRA_PINTO.pdf.txtJESSIKA_ANDREZA_OLIVEIRA_PINTO.pdf.txtExtracted texttext/plain180764https://ri.ufs.br/jspui/bitstream/riufs/17970/5/JESSIKA_ANDREZA_OLIVEIRA_PINTO.pdf.txtc9c5a6efde0d5eb44461cbae81b0201cMD55THUMBNAILJÉSSIKA_ANDREZA_OLIVEIRA_PINTO.pdf.jpgJÉSSIKA_ANDREZA_OLIVEIRA_PINTO.pdf.jpgGenerated Thumbnailimage/jpeg1355https://ri.ufs.br/jspui/bitstream/riufs/17970/4/J%c3%89SSIKA_ANDREZA_OLIVEIRA_PINTO.pdf.jpga9f77dc89335b5f37d11a025c9ab0c52MD54JESSIKA_ANDREZA_OLIVEIRA_PINTO.pdf.jpgJESSIKA_ANDREZA_OLIVEIRA_PINTO.pdf.jpgGenerated Thumbnailimage/jpeg1355https://ri.ufs.br/jspui/bitstream/riufs/17970/6/JESSIKA_ANDREZA_OLIVEIRA_PINTO.pdf.jpga9f77dc89335b5f37d11a025c9ab0c52MD56riufs/179702023-08-08 18:36:59.502oai:ufs.br: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Repositório InstitucionalPUBhttps://ri.ufs.br/oai/requestrepositorio@academico.ufs.bropendoar:2023-08-08T21:36:59Repositório Institucional da UFS - Universidade Federal de Sergipe (UFS)false |
dc.title.pt_BR.fl_str_mv |
Análises química, antioxidante e e citotoxicidade em linhagens tumorais de germoplasma de Eplingiella fruticosa |
title |
Análises química, antioxidante e e citotoxicidade em linhagens tumorais de germoplasma de Eplingiella fruticosa |
spellingShingle |
Análises química, antioxidante e e citotoxicidade em linhagens tumorais de germoplasma de Eplingiella fruticosa Pinto, Jéssika Andreza Oliveira Eplingiella fruticosa Óleo essencial Extratos Atividade biológica Alecrim-de-vaqueiro Essential oil Biological activity Extracts CIENCIAS AGRARIAS |
title_short |
Análises química, antioxidante e e citotoxicidade em linhagens tumorais de germoplasma de Eplingiella fruticosa |
title_full |
Análises química, antioxidante e e citotoxicidade em linhagens tumorais de germoplasma de Eplingiella fruticosa |
title_fullStr |
Análises química, antioxidante e e citotoxicidade em linhagens tumorais de germoplasma de Eplingiella fruticosa |
title_full_unstemmed |
Análises química, antioxidante e e citotoxicidade em linhagens tumorais de germoplasma de Eplingiella fruticosa |
title_sort |
Análises química, antioxidante e e citotoxicidade em linhagens tumorais de germoplasma de Eplingiella fruticosa |
author |
Pinto, Jéssika Andreza Oliveira |
author_facet |
Pinto, Jéssika Andreza Oliveira |
author_role |
author |
dc.contributor.author.fl_str_mv |
Pinto, Jéssika Andreza Oliveira |
dc.contributor.advisor1.fl_str_mv |
Arrigoni-Blank, Maria de Fátima |
contributor_str_mv |
Arrigoni-Blank, Maria de Fátima |
dc.subject.por.fl_str_mv |
Eplingiella fruticosa Óleo essencial Extratos Atividade biológica Alecrim-de-vaqueiro |
topic |
Eplingiella fruticosa Óleo essencial Extratos Atividade biológica Alecrim-de-vaqueiro Essential oil Biological activity Extracts CIENCIAS AGRARIAS |
dc.subject.eng.fl_str_mv |
Essential oil Biological activity Extracts |
dc.subject.cnpq.fl_str_mv |
CIENCIAS AGRARIAS |
description |
Eplingiella fruticosa is an aromatic plant of the Lamiaceae family known as “alecrim-devaqueiro”. Its popular use has aroused the interest of the scientific community regarding the possible pharmacological properties involved, and the anti-hyperalgesic, antinociceptive, antiinflammatory, vasodilatory and anti-neurodegenerative effects have already been attributed to its essential oil and extracts. The first article investigated the variations in the chemical profiles and biological activities (antioxidant and cytotoxicity) of Eplingiella fruticosa from the State of Sergipe. The essential oils of six populations were extracted by hydrodistillation and analyzed by Gas Chromatography coupled to a Mass Spectrometer and a Flame Ionization Detector (GC/EM-DIC). The antioxidant activity of the essential oils was tested by different assays (DPPH, ABTS, β-carotene and FRAP) and the cytotoxic activity was determined by the SRB assay. The compounds found in greater amounts were: α-pinene, β-pinene, 1,8-cineole, camphor, borneol, δ-elemene, α-cubebene, α-ylangeno, (E)-caryophyllene, germacrene D, bicyclogermacrene, trans -calamenene, spathulenol, caryophyllene oxide and viridiflorol. These compounds defined the formation of two groups. The first group consisted of the populations of São Cristóvão, Itaporanga, Japaratuba and Malhada dos Bois and was characterized by the presence of camphor monoterpene (8.39-11.27%) as the highest content compound in relation to the other municipalities. The second group consisted of the populations of Moita Bonita and Pirambu and was characterized by the majority presence of bicyclogermacrene sesquiterpene (7.45% and 10.98%). The plants showed weak effects in terms of antioxidant activity, but the essential oil showed significant toxicity for the A549 strains (51.00% cell viability) in the population of Japaratuba and B16F10 (64.94.6% of cell viability) in Malhada of the Oxen. The second article analyzed the chemical profile, antioxidant activity and cytotoxicity of aqueous and ethanolic extracts from populations of E. fruticosa. The extracts were analyzed by High Performance Liquid Chromatography (HPLC). Antioxidant activity and cytotoxic activity were tested in the same way as in the first article. The flavonoid quercitrin was identified in the ethanol extract. The aqueous extracts showed higher antioxidant activity in relation to the other extracts. The DPPH and ABTS radicals range from 84.89- 75.62% and 85.21-64.04%, respectively, in the aqueous extracts. The ethanol extracts from the populations of Japaratuba, São Cristóvão and Itaporanga d'Ajuda showed the highest inhibitory activities with 90.63%, 90.53% and 88.47% for the C6 strain and the hydrodistilled ethanol extract from the Japaratuba population showed high growth inhibition at A549 (87.17% cell viability) and C6 (72.83% cell viability). The observations from these studies show that E. fruticosa can be a significant source of medicinally important natural compounds. |
publishDate |
2022 |
dc.date.issued.fl_str_mv |
2022-01-28 |
dc.date.accessioned.fl_str_mv |
2023-07-28T22:28:15Z |
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2023-07-28T22:28:15Z |
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info:eu-repo/semantics/doctoralThesis |
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dc.identifier.citation.fl_str_mv |
PINTO, Jéssika Andreza Oliveira. Análises química, antioxidante e citotoxicidade em linhagens tumorais de germoplasma de Eplingiella fruticosa. 2022. 66 f. Tese (Doutorado em Agricultura e Biodiversidade) - Universidade Federal de Sergipe, São Cristóvão, SE, 2022. |
dc.identifier.uri.fl_str_mv |
https://ri.ufs.br/jspui/handle/riufs/17970 |
identifier_str_mv |
PINTO, Jéssika Andreza Oliveira. Análises química, antioxidante e citotoxicidade em linhagens tumorais de germoplasma de Eplingiella fruticosa. 2022. 66 f. Tese (Doutorado em Agricultura e Biodiversidade) - Universidade Federal de Sergipe, São Cristóvão, SE, 2022. |
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Universidade Federal de Sergipe |
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