Alterações motoras, cognitivas e neuroquímicas causadas pela administração repetida da deltametrina em ratos

Detalhes bibliográficos
Autor(a) principal: Souza, Marina Freire de
Data de Publicação: 2015
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Institucional da UFS
Texto Completo: https://ri.ufs.br/handle/riufs/3986
Resumo: Studies have shown that pesticide exposure is a risk factor to Parkinson Disease (PD) development in urban areas and mainly in rural areas. One of these pesticides, Deltamethrin (DM), is used indiscriminately for vector control in crops, veterinary medicine and control of domestic pests. The aim of the study is investigate the motor, cognitive and neurochemical changes caused by repeated administration of DM in rats. 38 Male Wistar rats were used, 9-10 months-old, from the Neurophysiology Laboratory animal house of UFS. The study was divided into two experiments: (1) intranasal (i.n.) and (2) intracerebroventricular (i.c.v.). In the (1), the animals were divided into 2 groups (CTR, 0.9% saline solution, n=9) and DM 0.5 (treated with DM 0.5 mg, n=10), that received 3 infusions administrated one every 7 days. During the treatment, the animal were subjected to behavior test: catalepsy, novel object recognition task, spontaneous alternation and contextual conditioned fear. In i.c.v. experiment, the animals were divided into 3 groups: control (CTR, n=7); DM 0.5 (treated with DM 0.5 μg diluted in 2 μL, n=7) and DM 5 (treated with DM 5 μg diluted in 2 μL, n=5). In the experiment (2), the animals received 3 i.c.v. injections (2 μL/injection), one each 48 hours. During the treatment, the rats were submitted to behavior test: catalepsy, open field test and spontaneous alternation. After the behavior tests of both experiments, the rats were anesthized, perfused transcardially, their brains removed and submitted to Tyrosine Hydroxylase (TH) immunochemistry in Substancia Nigra pars compacta (SNpc), Ventral Tegmental Area (VTA) and dorsal striatum areas. In i.n. experiment, there were motor changes in open field and cognitive changes in novel object recognition task and contextual conditioned fear. The immunohistochemistry shows a reduction of TH+ cells in SNpc and VTA and an increase of Optical Density in dorsal striatum. In i.c.v. experiment, it was observed motor changes in open field and cognitive changes in spontaneous alternation. In immunohistochemical there was a decrease of TH + cells in DM 5ug animals in SNpc and VTA and decreased their levels in DM 0.5 g and DM 5 ug groups in the dorsal striatum. In both experiments, cognitive changes preceded the motor changes. The data presented contribute to understanding of physiological and behavioral changes after exposure to DM and more studies are needed to elucidate the DM as a possible cause of parkinsonism symptoms.
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spelling Souza, Marina Freire deSANTOS, J. R.Santos, Jose Ronaldo doshttp://lattes.cnpq.br/81656074086149562017-09-26T12:31:18Z2017-09-26T12:31:18Z2015-06-28SOUZA, Marina Freire de. Motor, cognitive and neurochemical changes caused by repeated administration of deltamethrin in rats. 2015. 73 f. Dissertação (Mestrado em Ciências Fisiológicas) - Universidade Federal de Sergipe, São Cristóvão, 2015.https://ri.ufs.br/handle/riufs/3986Studies have shown that pesticide exposure is a risk factor to Parkinson Disease (PD) development in urban areas and mainly in rural areas. One of these pesticides, Deltamethrin (DM), is used indiscriminately for vector control in crops, veterinary medicine and control of domestic pests. The aim of the study is investigate the motor, cognitive and neurochemical changes caused by repeated administration of DM in rats. 38 Male Wistar rats were used, 9-10 months-old, from the Neurophysiology Laboratory animal house of UFS. The study was divided into two experiments: (1) intranasal (i.n.) and (2) intracerebroventricular (i.c.v.). In the (1), the animals were divided into 2 groups (CTR, 0.9% saline solution, n=9) and DM 0.5 (treated with DM 0.5 mg, n=10), that received 3 infusions administrated one every 7 days. During the treatment, the animal were subjected to behavior test: catalepsy, novel object recognition task, spontaneous alternation and contextual conditioned fear. In i.c.v. experiment, the animals were divided into 3 groups: control (CTR, n=7); DM 0.5 (treated with DM 0.5 μg diluted in 2 μL, n=7) and DM 5 (treated with DM 5 μg diluted in 2 μL, n=5). In the experiment (2), the animals received 3 i.c.v. injections (2 μL/injection), one each 48 hours. During the treatment, the rats were submitted to behavior test: catalepsy, open field test and spontaneous alternation. After the behavior tests of both experiments, the rats were anesthized, perfused transcardially, their brains removed and submitted to Tyrosine Hydroxylase (TH) immunochemistry in Substancia Nigra pars compacta (SNpc), Ventral Tegmental Area (VTA) and dorsal striatum areas. In i.n. experiment, there were motor changes in open field and cognitive changes in novel object recognition task and contextual conditioned fear. The immunohistochemistry shows a reduction of TH+ cells in SNpc and VTA and an increase of Optical Density in dorsal striatum. In i.c.v. experiment, it was observed motor changes in open field and cognitive changes in spontaneous alternation. In immunohistochemical there was a decrease of TH + cells in DM 5ug animals in SNpc and VTA and decreased their levels in DM 0.5 g and DM 5 ug groups in the dorsal striatum. In both experiments, cognitive changes preceded the motor changes. The data presented contribute to understanding of physiological and behavioral changes after exposure to DM and more studies are needed to elucidate the DM as a possible cause of parkinsonism symptoms.Estudos tem demonstrado que a exposição a pesticidas é um fator de risco para o desenvolvimento da doença de Parkinson (DP) em áreas urbanas e principalmente em áreas rurais. Um desses pesticidas, a deltametrina (DM), é utilizada indiscriminadamente no controle de vetores na lavoura, na medicina veterinária e no controle de pestes domésticas. Diante disso, o objetivo da pesquisa é avaliar as alterações motoras, cognitivas e neuroquímicas causadas pela administração repetida da DM em ratos. Foram utilizados 38 ratos Wistar machos, com idade entre 9-10 meses, provenientes do Biotério do Laboratório de Neurofisiologia da UFS. O trabalho foi dividido em dois experimentos: (1) administração intranasal (i.n.) e (2) intracerebroventricular (i.c.v.) de DM. No experimento 1 (intranasal), os animais foram divididos em 2 grupos: controle (CTR, solução salina 0,9%, n=9) e DM 0,5 (tratados com DM 0,5 mg, n=10), que receberam 3 infusões intranasais administradas 1 a cada 7 dias. Durante o tratamento, os animais foram submetidos a testes comportamentais: catalepsia, reconhecimento de objeto novo, alternação espontânea e medo condicionado ao contexto. No experimento 2 (i.c.v.), os animais foram divididos em 3 grupos: controle (CTR, n=7), DM 0,5 (tratados com DM 0,5 μg em 2 μL, n=7) e DM 5 (tratados com DM 5 μg em 2 μL, n=5). Nessa etapa, os animais receberam 3 injeções i.c.v. (2 μL por injeção), uma a cada 48h. Ao longo do tratamento, os ratos foram avaliados nos testes da catalepsia, campo aberto e alternação espontânea. Após os testes de comportamento em ambos experimentos, os ratos foram anestesiados, perfundidos, seus cérebros removidos e submetidos a imunohistoquímica para Tirosina Hidroxilase (TH) na substância negra parte compacta, área tegmental ventral e estriado dorsal. Foi observado no experimento i.n. que houve alterações motoras no campo aberto e cognitivas no reconhecimento de objeto novo e no medo condicionado ao contexto. A imunohistoquímica mostrou redução de células TH+ na SNpc e VTA e aumento de TH+ no estriado dorsal. No experimento i.c.v., foram observadas alterações motoras no campo aberto e cognitivas na alternação espontânea. Na imunohistoquímica houve diminuição de células TH+ nos animais DM 5 μg na SNpc e em VTA e redução de seus níveis nos grupos DM 0,5 μg e DM 5 μg no estriado dorsal. Em ambos os experimentos, alterações cognitivas precederam as alterações motoras. Os dados apresentados contribuem no entendimento sobre alterações fisiológicas e comportamentais após exposição a DM e mais estudos deverão ser realizados para elucidar a DM como um possível causador de sintomas de parkinsonismo.Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorapplication/pdfporUniversidade Federal de SergipePós-Graduação em Ciências FisiológicasUFSBRFisiologiaDoença de ParkinsonPesticidasDopaminaPiretróidesParkinsonismPesticidePyrethroidDopamineCNPQ::CIENCIAS BIOLOGICAS::FISIOLOGIAAlterações motoras, cognitivas e neuroquímicas causadas pela administração repetida da deltametrina em ratosMotor, cognitive and neurochemical changes caused by repeated administration of deltamethrin in ratsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFSinstname:Universidade Federal de Sergipe (UFS)instacron:UFSTEXTMARINA_FREIRE_SOUZA.pdf.txtMARINA_FREIRE_SOUZA.pdf.txtExtracted texttext/plain119712https://ri.ufs.br/jspui/bitstream/riufs/3986/2/MARINA_FREIRE_SOUZA.pdf.txt162d7b3209ab23f994d623a1380c20bbMD52THUMBNAILMARINA_FREIRE_SOUZA.pdf.jpgMARINA_FREIRE_SOUZA.pdf.jpgGenerated Thumbnailimage/jpeg1283https://ri.ufs.br/jspui/bitstream/riufs/3986/3/MARINA_FREIRE_SOUZA.pdf.jpg22da4b22987da7f692c1cd3ea8579c8cMD53ORIGINALMARINA_FREIRE_SOUZA.pdfapplication/pdf1274566https://ri.ufs.br/jspui/bitstream/riufs/3986/1/MARINA_FREIRE_SOUZA.pdf52b7986cc1a624955ebdb010dc77f107MD51riufs/39862017-11-24 21:44:13.757oai:ufs.br:riufs/3986Repositório InstitucionalPUBhttps://ri.ufs.br/oai/requestrepositorio@academico.ufs.bropendoar:2017-11-25T00:44:13Repositório Institucional da UFS - Universidade Federal de Sergipe (UFS)false
dc.title.por.fl_str_mv Alterações motoras, cognitivas e neuroquímicas causadas pela administração repetida da deltametrina em ratos
dc.title.alternative.eng.fl_str_mv Motor, cognitive and neurochemical changes caused by repeated administration of deltamethrin in rats
title Alterações motoras, cognitivas e neuroquímicas causadas pela administração repetida da deltametrina em ratos
spellingShingle Alterações motoras, cognitivas e neuroquímicas causadas pela administração repetida da deltametrina em ratos
Souza, Marina Freire de
Fisiologia
Doença de Parkinson
Pesticidas
Dopamina
Piretróides
Parkinsonism
Pesticide
Pyrethroid
Dopamine
CNPQ::CIENCIAS BIOLOGICAS::FISIOLOGIA
title_short Alterações motoras, cognitivas e neuroquímicas causadas pela administração repetida da deltametrina em ratos
title_full Alterações motoras, cognitivas e neuroquímicas causadas pela administração repetida da deltametrina em ratos
title_fullStr Alterações motoras, cognitivas e neuroquímicas causadas pela administração repetida da deltametrina em ratos
title_full_unstemmed Alterações motoras, cognitivas e neuroquímicas causadas pela administração repetida da deltametrina em ratos
title_sort Alterações motoras, cognitivas e neuroquímicas causadas pela administração repetida da deltametrina em ratos
author Souza, Marina Freire de
author_facet Souza, Marina Freire de
author_role author
dc.contributor.author.fl_str_mv Souza, Marina Freire de
dc.contributor.advisor1Lattes.fl_str_mv SANTOS, J. R.
dc.contributor.advisor1.fl_str_mv Santos, Jose Ronaldo dos
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/8165607408614956
contributor_str_mv Santos, Jose Ronaldo dos
dc.subject.por.fl_str_mv Fisiologia
Doença de Parkinson
Pesticidas
Dopamina
Piretróides
topic Fisiologia
Doença de Parkinson
Pesticidas
Dopamina
Piretróides
Parkinsonism
Pesticide
Pyrethroid
Dopamine
CNPQ::CIENCIAS BIOLOGICAS::FISIOLOGIA
dc.subject.eng.fl_str_mv Parkinsonism
Pesticide
Pyrethroid
Dopamine
dc.subject.cnpq.fl_str_mv CNPQ::CIENCIAS BIOLOGICAS::FISIOLOGIA
description Studies have shown that pesticide exposure is a risk factor to Parkinson Disease (PD) development in urban areas and mainly in rural areas. One of these pesticides, Deltamethrin (DM), is used indiscriminately for vector control in crops, veterinary medicine and control of domestic pests. The aim of the study is investigate the motor, cognitive and neurochemical changes caused by repeated administration of DM in rats. 38 Male Wistar rats were used, 9-10 months-old, from the Neurophysiology Laboratory animal house of UFS. The study was divided into two experiments: (1) intranasal (i.n.) and (2) intracerebroventricular (i.c.v.). In the (1), the animals were divided into 2 groups (CTR, 0.9% saline solution, n=9) and DM 0.5 (treated with DM 0.5 mg, n=10), that received 3 infusions administrated one every 7 days. During the treatment, the animal were subjected to behavior test: catalepsy, novel object recognition task, spontaneous alternation and contextual conditioned fear. In i.c.v. experiment, the animals were divided into 3 groups: control (CTR, n=7); DM 0.5 (treated with DM 0.5 μg diluted in 2 μL, n=7) and DM 5 (treated with DM 5 μg diluted in 2 μL, n=5). In the experiment (2), the animals received 3 i.c.v. injections (2 μL/injection), one each 48 hours. During the treatment, the rats were submitted to behavior test: catalepsy, open field test and spontaneous alternation. After the behavior tests of both experiments, the rats were anesthized, perfused transcardially, their brains removed and submitted to Tyrosine Hydroxylase (TH) immunochemistry in Substancia Nigra pars compacta (SNpc), Ventral Tegmental Area (VTA) and dorsal striatum areas. In i.n. experiment, there were motor changes in open field and cognitive changes in novel object recognition task and contextual conditioned fear. The immunohistochemistry shows a reduction of TH+ cells in SNpc and VTA and an increase of Optical Density in dorsal striatum. In i.c.v. experiment, it was observed motor changes in open field and cognitive changes in spontaneous alternation. In immunohistochemical there was a decrease of TH + cells in DM 5ug animals in SNpc and VTA and decreased their levels in DM 0.5 g and DM 5 ug groups in the dorsal striatum. In both experiments, cognitive changes preceded the motor changes. The data presented contribute to understanding of physiological and behavioral changes after exposure to DM and more studies are needed to elucidate the DM as a possible cause of parkinsonism symptoms.
publishDate 2015
dc.date.issued.fl_str_mv 2015-06-28
dc.date.accessioned.fl_str_mv 2017-09-26T12:31:18Z
dc.date.available.fl_str_mv 2017-09-26T12:31:18Z
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dc.identifier.citation.fl_str_mv SOUZA, Marina Freire de. Motor, cognitive and neurochemical changes caused by repeated administration of deltamethrin in rats. 2015. 73 f. Dissertação (Mestrado em Ciências Fisiológicas) - Universidade Federal de Sergipe, São Cristóvão, 2015.
dc.identifier.uri.fl_str_mv https://ri.ufs.br/handle/riufs/3986
identifier_str_mv SOUZA, Marina Freire de. Motor, cognitive and neurochemical changes caused by repeated administration of deltamethrin in rats. 2015. 73 f. Dissertação (Mestrado em Ciências Fisiológicas) - Universidade Federal de Sergipe, São Cristóvão, 2015.
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