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Detalhes bibliográficos
Autor(a) principal: Marques, Rodrigo da Silva
Data de Publicação: 2007
Outros Autores: Vulcano, Mirella, Cazerta, Susana M. M., Miglino, Maria Angélica, Assis Neto, Antônio Chaves de, Pereira, Flávia Thomaz Verechia
Tipo de documento: Artigo
Idioma: por
Título da fonte: Biotemas (Online)
Texto Completo: https://periodicos.ufsc.br/index.php/biotemas/article/view/20677
Resumo: Morphological characterization of the intercaruncular region of pregnant cows and water buffalo cows. Intercaruncular glandular regions were found among the uterine caruncles in ruminant pregnant uteruses. The endometrial glands play an important role in the substance transfer from dam to fetus, including that of iron. Therefore, our objective was to characterize these intercaruncular regions of the pregnant uterus of zebu and buffalo cows. Small portions of the same regions of zebu (n=57) and water buffalo cows (n=30) were fixed in 10% formaldehyde and 4% paraformoldehyde in PBS, processed and stained for light microscopy in order to characterize the morphology, and histochemistry (Perl’s, PAS and acid phosphatase reactions) to confirm iron transfer. The intercaruncular region was located among the uterine caruncles and was composed by an endometrium, a myometrium, and a richly vascularized perimetrium. The endometrial glands were made up of a simple columnar epithelium and they were reactive to ferric iron (Perl's reaction), acid phosphatase and PAS. Collectively, the present results support the hypothesis that endometrial glands are important sites of secretion and substance transfer from the dam and that they are implicated in the nourishment of the ruminant fetus.
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spelling .Caracterização morfológica da região intercaruncular uterina de vacas e búfalas gestantesMorphological characterization of the intercaruncular region of pregnant cows and water buffalo cows. Intercaruncular glandular regions were found among the uterine caruncles in ruminant pregnant uteruses. The endometrial glands play an important role in the substance transfer from dam to fetus, including that of iron. Therefore, our objective was to characterize these intercaruncular regions of the pregnant uterus of zebu and buffalo cows. Small portions of the same regions of zebu (n=57) and water buffalo cows (n=30) were fixed in 10% formaldehyde and 4% paraformoldehyde in PBS, processed and stained for light microscopy in order to characterize the morphology, and histochemistry (Perl’s, PAS and acid phosphatase reactions) to confirm iron transfer. The intercaruncular region was located among the uterine caruncles and was composed by an endometrium, a myometrium, and a richly vascularized perimetrium. The endometrial glands were made up of a simple columnar epithelium and they were reactive to ferric iron (Perl's reaction), acid phosphatase and PAS. Collectively, the present results support the hypothesis that endometrial glands are important sites of secretion and substance transfer from the dam and that they are implicated in the nourishment of the ruminant fetus.As regiões glandulares denominadas intercarunculares foram encontradas entre as carúnculas uterinas no útero gestante de ruminantes. As glândulas endometriais desempenham importante função de transferência de substâncias da mãe para o feto, incluindo o ferro. Sendo assim, tivemos por objetivo caracterizar estas regiões intercarunculares do útero gestante de vacas zebuínas e búfalas. Amostras destas mesmas regiões de vacas zebuínas (n=57) e búfalas (n=30), em vários estágios de gestação, foram fixadas em solução aquosa de formaldeído a 10% e paraformaldeído a 4% em PBS, processadas e coradas para microscopia de luz, para caracterizar a morfologia e histoquímica (reações de Perl's, PAS e fosfatase ácida), para confirmar a transferência de ferro. A região intercaruncular situou-se entre as carúnculas uterinas e foi composta pelo endométrio, miométrio e perimétrio altamente vascularizado. As glândulas endometriais eram constituídas por epitélio prismático simples e reagiram positivamente ao ferro férrico (reação de Perl’s), à enzima fosfatase ácida e à reação do PAS. Analisados conjuntamente, os presentes resultados sustentam a hipótese de que as glândulas endometriais são sítios importantes de secreção e transferência de substâncias da mãe, implicadas na nutrição histiotrófica dos fetos de ruminantes, principalmente as proteínas que agem como enzimas, carreadores de moléculas para hormônios, vitaminas e minerais e como moléculas sinalizadoras do reconhecimento da gestação.Universidade Federal de Santa Catarina2007-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://periodicos.ufsc.br/index.php/biotemas/article/view/20677Biotemas; v. 20 n. 3 (2007); 103-1142175-79250103-1643reponame:Biotemas (Online)instname:Universidade Federal de Santa Catarina (UFSC)instacron:UFSCporhttps://periodicos.ufsc.br/index.php/biotemas/article/view/20677/18833Copyright (c) 2007 Rodrigo da Silva Marques, Mirella Vulcano, Susana M. M. Cazerta, Maria Angélica Miglino, Antônio Chaves de Assis Neto, Flávia Thomaz Verechia Pereirahttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessMarques, Rodrigo da SilvaVulcano, MirellaCazerta, Susana M. M.Miglino, Maria AngélicaAssis Neto, Antônio Chaves dePereira, Flávia Thomaz Verechia2022-11-21T12:22:42Zoai:periodicos.ufsc.br:article/20677Revistahttp://www.biotemas.ufsc.br/index.htmPUBhttps://periodicos.ufsc.br/index.php/biotemas/oai||carlospinto@ccb.ufsc.br2175-79250103-1643opendoar:2022-11-21T12:22:42Biotemas (Online) - Universidade Federal de Santa Catarina (UFSC)false
dc.title.none.fl_str_mv .
Caracterização morfológica da região intercaruncular uterina de vacas e búfalas gestantes
title .
spellingShingle .
Marques, Rodrigo da Silva
title_short .
title_full .
title_fullStr .
title_full_unstemmed .
title_sort .
author Marques, Rodrigo da Silva
author_facet Marques, Rodrigo da Silva
Vulcano, Mirella
Cazerta, Susana M. M.
Miglino, Maria Angélica
Assis Neto, Antônio Chaves de
Pereira, Flávia Thomaz Verechia
author_role author
author2 Vulcano, Mirella
Cazerta, Susana M. M.
Miglino, Maria Angélica
Assis Neto, Antônio Chaves de
Pereira, Flávia Thomaz Verechia
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Marques, Rodrigo da Silva
Vulcano, Mirella
Cazerta, Susana M. M.
Miglino, Maria Angélica
Assis Neto, Antônio Chaves de
Pereira, Flávia Thomaz Verechia
description Morphological characterization of the intercaruncular region of pregnant cows and water buffalo cows. Intercaruncular glandular regions were found among the uterine caruncles in ruminant pregnant uteruses. The endometrial glands play an important role in the substance transfer from dam to fetus, including that of iron. Therefore, our objective was to characterize these intercaruncular regions of the pregnant uterus of zebu and buffalo cows. Small portions of the same regions of zebu (n=57) and water buffalo cows (n=30) were fixed in 10% formaldehyde and 4% paraformoldehyde in PBS, processed and stained for light microscopy in order to characterize the morphology, and histochemistry (Perl’s, PAS and acid phosphatase reactions) to confirm iron transfer. The intercaruncular region was located among the uterine caruncles and was composed by an endometrium, a myometrium, and a richly vascularized perimetrium. The endometrial glands were made up of a simple columnar epithelium and they were reactive to ferric iron (Perl's reaction), acid phosphatase and PAS. Collectively, the present results support the hypothesis that endometrial glands are important sites of secretion and substance transfer from the dam and that they are implicated in the nourishment of the ruminant fetus.
publishDate 2007
dc.date.none.fl_str_mv 2007-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
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dc.identifier.uri.fl_str_mv https://periodicos.ufsc.br/index.php/biotemas/article/view/20677
url https://periodicos.ufsc.br/index.php/biotemas/article/view/20677
dc.language.iso.fl_str_mv por
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dc.relation.none.fl_str_mv https://periodicos.ufsc.br/index.php/biotemas/article/view/20677/18833
dc.rights.driver.fl_str_mv http://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Santa Catarina
publisher.none.fl_str_mv Universidade Federal de Santa Catarina
dc.source.none.fl_str_mv Biotemas; v. 20 n. 3 (2007); 103-114
2175-7925
0103-1643
reponame:Biotemas (Online)
instname:Universidade Federal de Santa Catarina (UFSC)
instacron:UFSC
instname_str Universidade Federal de Santa Catarina (UFSC)
instacron_str UFSC
institution UFSC
reponame_str Biotemas (Online)
collection Biotemas (Online)
repository.name.fl_str_mv Biotemas (Online) - Universidade Federal de Santa Catarina (UFSC)
repository.mail.fl_str_mv ||carlospinto@ccb.ufsc.br
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